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Citations for Thermo Fisher :
2351 - 2400 of 10000+ citations for 5 Bromo 2 Tert Butyldimethylsilyl 4 Methylthiazole since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2023Quote: ... the cell nuclei were stained with 4′,6-diamidino-2-phenylindole (DAPI, 1 μg/mL, Thermo Fisher Scientific, Waltham, MA, USA) in PBS for 15 minutes at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... then incubated for 2 hours at 4°C in a 1:1000 solution of AF-568 donkey anti-mouse secondary antibody (Invitrogen #A10037) in blocking solution ...
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bioRxiv - Biochemistry 2023Quote: ... 10 µL was loaded onto an IonPac AS-11 HC strong anion-exchange analytical column (2 mm × 250 mm, 4 µm particle diameter, Thermo Scientific) with AG-11 HC guard column (2 mm × 50 mm ...
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bioRxiv - Microbiology 2023Quote: ... Cell lysates were then mixed with 2 x of the NuPAGE™ LDS Sample Buffer 4 x (Novex, Life Technologies, USA) with addition of 10% β-mercapto-ethanol and incubated for 5 min at 98 °C ...
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Mitochondrial Apolipoprotein MIC26 is a metabolic rheostat regulating central cellular fuel pathwaysbioRxiv - Cell Biology 2023Quote: ... Anion exchange chromatography was conducted on a Dionex IonPac AS11-HC column (2 mm X 250 mm, 4 µm particle size, Thermo Scientific) equipped with a Dionex IonPac AG11-HC guard column (2 mm X 50 mm ...
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bioRxiv - Cell Biology 2023Quote: ... additionally this was equipped with a Dionex IonPac AG11-HC guard column (2 mm × 50 mm, 4 μm, Thermo Fisher Scientific). The column temperature was held at 30°C and the auto sampler was set to a temperature of 6°C ...
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bioRxiv - Microbiology 2023Quote: ... sections were counterstained with 1 µg/ml of 4’,6-diamidino-2-phenylindole (DAPI, #D1306, Thermo Fisher Scientific, Waltham, MA, USA) then ...
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bioRxiv - Neuroscience 2024Quote: The pipette solution (EGTA omitted) was supplemented with 100 μM calcium-sensitive dye Fluo-4 or Fura-2 (Thermo Fisher Scientific) and 50 μM calcium-insensitive dye Alexa Fluor 568 (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were washed once with PBS and a mixture of 1:1:2 (w/w) of pcDNA5-αSyn (4 μg): pOG44 Flp-recombinase expression vector (pOG44, Invitrogen #V600520):PEI in 2 mL of DMEM was added for 8 h ...
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bioRxiv - Molecular Biology 2024Quote: ... coverslips were mounted on glass slides using ProLong Gold antifade (4′,6-diamidino-2-phenylindole) DAPI to counterstain the nuclei (Thermofisher, #P36935).
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bioRxiv - Biochemistry 2024Quote: ... The remaining 45 μL were added to 2 μL of settled anti-Flag magnetic beads (4 μL of 50% slurry) (Thermo Fisher) that had been equilibrated in Wash Buffer ...
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bioRxiv - Physiology 2024Quote: ... Cells were loaded at 37°C for 60 minutes with 4 μM Fura-2-AM in the presence of 1X Power Load permeabilizing reagent (Thermo Fisher). Injection of AngII (10-8M) ...
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bioRxiv - Cell Biology 2024Quote: ... washed with PBS and stained with a combination of Alexa Fluor secondary antibodies (Thermo) and 4’,6-Diamidino-2-Phenylindole (DAPI; Molecular Probes) for 30 min at RT ...
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bioRxiv - Molecular Biology 2024Quote: ... the media was removed and cells were stained with 4’,6-diamidino-2-phenylindole (DAPI) at a concentration of 1 µg/mL (ThermoFisher Scientific) in Phosphate-Buffered Saline (PBS ...
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bioRxiv - Microbiology 2024Quote: ... They were then pelleted on a tabletop centrifuge at 12.000g for 2 min at room temperature and resuspended to OD 10 with LB containing 4 μg/mL FM4-64 (T13320, ThermoFisher Scientific) and incubated for 10 min at room temperature in the dark ...
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bioRxiv - Immunology 2024Quote: ... with 2 ceramic beads (2.8mm; Fisherbrand™ Bulk beads, 15-340-160) and 500 µl cold (4°C) Trizol reagent (Invitrogen, 15596018). Samples were then homogenized (Fisherbrand™ Bead Mill 24 Homogenizer ...
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bioRxiv - Immunology 2024Quote: ... the reaction mixture was dialyzed against deionized water at 4 °C for 2 days using Slide-A-LyzerTM Dialysis Cassette (10K MWCO, 3 mL, Thermo Scientific) and stored at −70 °C.
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Novel RNA-binding protein YebC enhances translation of proline-rich amino acid stretches in bacteriabioRxiv - Molecular Biology 2024Quote: ... 2 µL of the reactions were resolved on a NuPAGE 4-12% Bis-Tris Protein Gel with MES buffer (Thermo Scientific) and transferred to a 0.2 µm nitrocellulose membrane (Cytiva ...
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bioRxiv - Cancer Biology 2024Quote: ... RIPA Lysis and Extraction Buffer (CAT NO. 89901), and DAPI (4’,6-Diamidino-2-Phenylindole, Dilactate) (CAT NO. D3571) were purchased from Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... further supplemented with 10% (v/v) heat-inactivated fetal bovine serum (FBS) and 25 mM 4-(2-hydroxyethyl)-1- piperazineethanesulfonic acid (HEPEs) buffer (Gibco, US), 2.5g/l Glucose (Merck ...
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bioRxiv - Microbiology 2024Quote: ... unpermeabilised cells were blocked with 2% FBS/PBS for one hour with immunostaining performed as above with the inclusion of 4′,6-diamidino-2-phenylindole (DAPI, Thermo Fisher) [1:1000].
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bioRxiv - Biophysics 2024Quote: ... Surplus sample was removed by blotting for 2 to 4 s using a blot force of 10 in a Vitrobot Mark IV (Thermo Fisher Scientific ...
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bioRxiv - Physiology 2024Quote: ... Analytical separation was then performed at a flow rate of 250 nl/min over a 95 min gradient of 4-25% acetonitrile using a 2 µm EASY-Spray PepMap RSLC C18 75 µm x 250 mm column (ES802A, Thermo Fisher) with a column temperature of 45°C ...
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bioRxiv - Immunology 2024Quote: ... were coated with 1 µg/mL (full dose, or diluted to 1/2, 1/4, 1/8) of anti-CD3 antibody (16-0032-81, Thermo Fisher) in PBS at 4 °C overnight ...
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bioRxiv - Immunology 2024Quote: ... new aliquots of 2×106 or 4×106 conidia per mL are prepared with RPMI-1640 (Gibco® Cat. 11875-093) and incubated at 37°C +5% CO2 for 3 h ...
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bioRxiv - Systems Biology 2024Quote: ... and incubated in total of 150 µL SH2 binding buffer containing 20 µM biotinylated SH2 domain on a rotator and 4 °C for 2-3 hours in low protein binding microcentrifuge tubes (1.5 mL, Thermo Scientific™). The functionalized magnetic beads are then washed twice in 1 mL of SH2 binding buffer ...
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bioRxiv - Bioengineering 2024Quote: ... Nuclei and F-actin were stained by incubation with 4′,6-diamidino-2-phenylindole (DAPI, 1 μg/mL, Thermo Fisher Scientific) and phalloidin–tetramethylrhodamine B isothiocyanate (phalloidin-TRITC ...
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bioRxiv - Bioengineering 2024Quote: ... A total of 4 μg of protein were extracted from each lysate and reduced with 1:1 dilution of 2-mercaptoethanol (Thermo Scientific) and 2× Laemilli Buffer (Biorad) ...
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bioRxiv - Bioengineering 2024Quote: Samples were washed 3 times with DPBS before being incubated with secondary antibodies and 4′,6-diamidino-2-phenylindole (DAPI, Invitrogen, 10125092) at 1:500 in the blocking solution ...
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bioRxiv - Cell Biology 2024Quote: ... The PA gel surface was functionalized with 1 mg/ml of Sulfo-SANPAH (sulfosuccinimidyl-6-(4-azido-2-nitrophenylamino) hexanoate) in warm 50 mM HEPES buffer (Life Technologies) for immobilization of 100 μg/ml collagen type I (PureCol ...
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bioRxiv - Cell Biology 2024Quote: ... the slides were washed in 1 x PBS and covered with 4’,6-diamidino-2-phenylindole (DAPI) containing the antifade reagent ProlongGold (Life Technologies). Images for Protein A fusion proteins were obtained using camera Olympus DP73 (Axioplan 2 imaging) ...
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bioRxiv - Cell Biology 2020Quote: ... supplemented with 4 μM Mag-Fluo-4 (ThermoFisher Scientific) and 1 μM F-127 (ThermoFisher Scientific ...
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bioRxiv - Cancer Biology 2021Quote: ... Peptides were trapped at 20 μL/min in loading solvent (2% acetonitrile, 0.05% TFA) on a 5 mm × 300 μm C18 PepMap cartridge pre-column (Thermo Fisher Scientific / Dionex Softron GmbH ...
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bioRxiv - Systems Biology 2020Quote: ... fixed and blocked HeLa CCL-2 cells were incubated with 5 μg/mL rabbit anti-Ki67 (Thermo Fisher Scientific; RB1510P1ABX) in 1X blocking buffer for 45 min ...
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bioRxiv - Systems Biology 2020Quote: ... fixed and blocked HeLa CCL-2 cells were incubated with 5 μg/mL rabbit anti-Ki67 (Thermo Fisher Scientific; RB1510P1ABX) for 45 min in 1X blocking buffer ...
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bioRxiv - Cell Biology 2020Quote: We seeded 2×105 cells / well into 6 well dishes and replaced media with 5% KSR (Knockout Serum Replacement) (Gibco) containing media the next day ...
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bioRxiv - Cell Biology 2020Quote: ... 5 μm particle size) and an analytical column (PepMap C18, 500 × 0.075 μm, 2 μm particle size, Thermo Fisher Scientific), coupled to a Linear Trap Quadrupole Orbitrap Velos (with CID ...
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bioRxiv - Cell Biology 2020Quote: ... Samples were washed three times with PBS 0.1% Triton X-100 for 5 minutes and nuclei were counterstained with 2 μg/μl Hoechst 33342 (Invitrogen H3570) in PBS 0.1% Triton X-100 for 5 minutes at RT ...
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bioRxiv - Evolutionary Biology 2021Quote: ... freshly cultured hESC colonies were dissociated into clumps (2-5 cells) with Versene buffer (with EDTA, Gibco, Cat. No. 15040066). The dissociated cells were seeded on Geltrex-coated 6-well tissue culture plates at 25,000 cells/cm2 (0.25M per well in the 6-well plates ...
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bioRxiv - Immunology 2022Quote: ... The cells were individually collected using an automated micro-manipulating system (CellCelector, ALS) and deposited in nuclease-free microtubes containing 5 μL of 2× CellsDirect buffer and RNase Inhibitor (Invitrogen). Single cell RT-qPCR was then performed using the protocol ADP41 developed by Fluidigm ...
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bioRxiv - Molecular Biology 2021Quote: ... in Minimum essential medium (MEM), glutamine-free based Neuronal growth medium (5% fetal bovine serum, 2% B27, 1% Glutamax (100×, Invitrogen), 2 % 1 mol/L d-glucose ...
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bioRxiv - Molecular Biology 2021Quote: ... We labeled the purified RLC with 10 molar excess of 5-((((2-Iodoacetyl)amino)ethyl)amino)naphthalene-1-sulfonic acid (IAEDANS, Invitrogen) or dabcyl C2 maleimide (AnaSpec ...
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bioRxiv - Biochemistry 2020Quote: ... 5 μm) and a C18 analytical column (PepMapAcclaim C18, 50 cm x 075 mm, 2 μm, Dionex-Thermo-Fisher Scientific), applying a linear gradient from 2 to 35 % solvent B (80 % acetonitrile ...
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bioRxiv - Genetics 2020Quote: ... and 2 μg of plasmid encoding tethered NHA-protein using 5 μl of Lipofectamine 2000 and OPTI-MEM media (Invitrogen) according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... neurons facing down, onto dishes containing the feeder layer of glia and 5 ml of culturing medium (94% Neurobasal, 2% Glutamax (Invitrogen), 2% B27 ...
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bioRxiv - Microbiology 2021Quote: Plasmodium falciparum blood stage parasites were maintained at 2% hematocrit in 1640 RPMI-HEPES supplemented with 5% AlbuMAX II (GIBCO) and 0.25% gentamycin ...
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bioRxiv - Bioengineering 2020Quote: ... The cells were passaged at a density of 1:2 to 1:8 after reaching ~80% confluency by detaching with 5 mM EDTA in PBS (Invitrogen 15575-038 diluted in sterile 1X PBS ...
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bioRxiv - Bioengineering 2020Quote: ... Primary fibroblast passages 2–6 were achieved in culture in 37°C in a 5% CO2 incubator with DMEM High Glucose (Gibco) with 10% FBS to 80% confluence in 100 mm Petri dishes ...
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bioRxiv - Neuroscience 2021Quote: ... samples were washed 5-6 times over 2 hours in PBT at room temperature and incubated with secondary antibodies (Invitrogen) diluted in NGS-PBT for 24 hours at 4°C ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were centrifuged at 130 × g for 5 min and the pellet was resuspended in culture medium consisting of Neurobasal medium with 2% B27 (Invitrogen), 1% L-Glutamine (Invitrogen ...