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Citations for Thermo Fisher :
2051 - 2100 of 10000+ citations for 5 Bromo 2 Tert Butyldimethylsilyl 4 Methylthiazole since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2024Quote: ... CHX-treated cells were harvested at different time points (0, 2, 4 and 6 hours) and processed for immunoblotting with anti-LSD1 (Invitrogen) and anti-RCOR1 (Abcam ...
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bioRxiv - Cancer Biology 2024Quote: ... and A-253 cells were serially diluted and seeded at three different dilutions (2, 4 and 128 cells/well) with 50 µL serum-free DMEM/F12 medium (Gibco) supplemented with 10 ng/ml human EGF (R & D systems) ...
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bioRxiv - Cell Biology 2024Quote: ... No mycoplasma were detected in cultures by 4′,6-diamidino-2-phenylindole (DAPI) or TO-PRO-3 (Thermo Fisher Scientific) staining ...
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bioRxiv - Microbiology 2024Quote: ... Transfectants were selected with either 4 nM WR99210 (Jacobus Pharmaceuticals; pSLI) or 2 μg/μl blasticidin S (Life Technologies; pSLI2). SLI for selection of parasites with the plasmid integrated into the genome was done as described (Birnbaum et al. ...
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bioRxiv - Plant Biology 2024Quote: ... CrGAP1-4 and CrGDI1-2 were PCR amplified from leaf cDNAs using Phusion high-fidelity DNA polymerase (Thermo Fisher Scientific) and gene-specific oligonucleotides ...
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bioRxiv - Molecular Biology 2024Quote: ... Gradients were ultracentrifuged at 36,000 rpm for 2 hours at 4°C using a TH-641 rotor (Thermo Fisher Scientific) and fractionated on a Piston Fractionator (BioComp).
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bioRxiv - Neuroscience 2024Quote: ... retinas were incubated with secondary antibodies at 4°C for another 2-3 days: goat anti-chicken antibody conjugated to Alexa Fluor 488 (1:1000; A11039, Invitrogen), goat anti-rabbit 555 (1:1000 ...
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bioRxiv - Microbiology 2024Quote: ... For buffered growth mDMEM-DMP was supplemented with 100 mM of 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES, Gibco 15630056).
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bioRxiv - Systems Biology 2024Quote: ... A subset of APCs was fixed for 30 minutes in 4% PFA and stained with 2 μM BODIPY (Thermo Fisher) and 20 μM Hoechst (Thermo Fisher) ...
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bioRxiv - Immunology 2024Quote: ... and 3 µg/mL (full dose, or diluted to 1/2, 1/4, 1/8) anti-CD28 (MA110172, Thermo Fisher), and cultured in the pre-coated plate for 3 days ...
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bioRxiv - Immunology 2024Quote: ... 2 to 4×106 CD8+ T cells were lysed in the lysis buffer provided and passed through a homogenizer (Invitrogen) before RNA purification following manufacturer instructions ...
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bioRxiv - Developmental Biology 2024Quote: ... for 2 hrs at RT and incubated overnight at 4°C in primary antibody solution (1:100 rabbit anti-GFP (ThermoFisher) in blocking reagent) ...
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bioRxiv - Bioengineering 2024Quote: ... the sections were rinsed in PBS and the nuclei were counterstained and mounted with Slowfade gold antifade mountant containing 4’,6-diamidino-2-phenylindole (DAPI; #S36939, Invitrogen). The slides were imaged at a 4X magnification using EVOS M7000 (ThermoFisher Scientific™ ...
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bioRxiv - Immunology 2024Quote: ... filtered through a 0.45 µm pore-sized filter to remove cell debris and concentrated by centrifugation at 100000 g for 2 h at 4°C (Thermofisher WX Ultra80 centrifuge ...
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bioRxiv - Microbiology 2024Quote: ... blocked with 2% BSA in a PBS buffer for 10 min and incubated with 0.1–1 μg/ml DAPI (4’, 6-diamidyno-2-fenyloindol, Molecular Probes) and WGA-Texas Red (Wheat Germ Agglutinin-Texas Red ...
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bioRxiv - Molecular Biology 2024Quote: ... 25 μg of protein (or all of the sample for the MDF multiplexing experiment across 2 gels) was loaded into a NuPAGE 4∼12% Bis-Tris 1.5 mm gel (Invitrogen #NP0335) and gel electrophoresis performed ...
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bioRxiv - Neuroscience 2024Quote: ... before incubation with DAPI (4’,6-diamidino-2-phenylindole, dilactate, 0.1 µg ml−1 final concentration in PB, Invitrogen D3571) in 0.1 M PB for 10–20 mins at RT ...
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bioRxiv - Neuroscience 2024Quote: ... microglia were plated on 25 mm coverslips coated with PLL (0.01%) and Collagen IV (4 µg/ml) and loaded with 10 µM Fura-2 AM (Thermo Fisher) with 0.02% Pluronic F-127 (Thermo Fisher ...
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bioRxiv - Biophysics 2024Quote: ... This was done by placing 0.2 mg/ml sulfo-succinimidyl-6-(4-azido-2-nitrophenyl-amino) hexanoate (sulfo-SANPAH) (Thermo Fisher) in 0.5 mM pH 8.5 HEPES buffer onto the hydrogel surface and irradiating with ultraviolet (UV ...
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bioRxiv - Synthetic Biology 2024Quote: ... DNA labeling was performed using PBS containing 1 µg/ml 4′,6-diamidino-2-phenylindole (DAPI) (1:10000) (Invitrogen, D1306). After 3 additional washes with PBS ...
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bioRxiv - Biochemistry 2024Quote: ... that was pre-conjugated to Protein A/G beads (Pierce, 88802) for 2 h 4 °C (note: pre-conjugation crosslinked using 20 mM dimethyl pimelimidate (DMP, ThermoFisher) in borate buffer (40 mM boric acid ...
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bioRxiv - Bioengineering 2024Quote: ... the cells were washed three times with permeabilization buffer and counterstained with 4′,6-diamidino-2-phenylindole (DAPI) (Invitrogen #D1306). The primary antibodies used included rabbit Anti-Brachyury (Abcam ...
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bioRxiv - Bioengineering 2024Quote: ... samples were washed again with PBST and incubated with 4′,6-diamidino-2-phenylindole (DAPI, Molecular Probes, 1:2000 dilution) and phalloidin-tetramethyl rhodamine B isothiocyanate (phalloidin-TRITC ...
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bioRxiv - Biochemistry 2024Quote: ... that was pre-conjugated to Protein A/G beads (Pierce, 88802) for 2 h 4 °C (note: pre-conjugation crosslinked using 20 mM dimethyl pimelimidate (DMP, ThermoFisher) in borate buffer (40 mM boric acid ...
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bioRxiv - Cell Biology 2024Quote: ... and 2x the concentration of all the other supplements used in R10 medium (see above) and either 2% or 4% of UltraPure Agarose (Invitrogen) dissolved in one part water to achieve different agarose stiffnesses ...
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bioRxiv - Cancer Biology 2022Quote: ... 10 μL of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT, concentration 5 mg/mL, Invitrogen, cat. M6494) in PBS was added to each well containing culture medium and incubated for 2.5 h at 37 °C ...
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bioRxiv - Biophysics 2021Quote: ... One liter of Sf9 cells (2 × 106/mL confluence) was infected with 5 mL P3 baculoviral stock obtained following the manufacturer’s instructions (Invitrogen). The medium containing secreted protein was collected on the 4th day (about 72-hour post-infection ...
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bioRxiv - Bioengineering 2021Quote: Each sample (n=5) was placed in a 2 mL centrifuge tube containing 320 μL of collagenase II (Gibco, Thermo Fisher Scientific Inc. ...
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bioRxiv - Bioengineering 2021Quote: ... #2) Sterilize the oxygen sensor by submerging the sensor tip in 70% ethanol (Thermo Fisher Scientific, 64-17-5) in an eppendorf tube for 10 sec ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Three tadpoles per stage (1, 2, 5, 7, and 8 weeks after hatching) were fixed in an RNAlater (Ambion) solution ...
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bioRxiv - Biochemistry 2020Quote: StNFeoB proteins (2-5 µM each) were incubated with a range of substrate (GTP for molecular biology, Thermo Scientific) concentrations (25 µM to 5000 µM) ...
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bioRxiv - Microbiology 2021Quote: ... A trap column (5 μm, 100 Å, 100 μm × 2 cm, Acclaim PepMap 100 nanoViper C18; Thermo Fisher Scientific) and an analytical column (2 μm ...
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bioRxiv - Neuroscience 2020Quote: Time plug-mated Pbx3−/- mice received a single intraperitoneal injection of 5-ethynyl-2’-deoxyuridine (EdU; 0,1mg/g animal; Invitrogen) at E15.5 ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were incubated for 5 min with 2 μM LysoSensor Yellow/Blue DND-160 (L7545, Invitrogen/Thermo Fisher Scientific) in extracellular Ringer’s solution at 37°C and 5% CO2 ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were incubated for 5 min with 2 μM LysoSensor Yellow/Blue DND-160 (L7545, Invitrogen/Thermo Fisher Scientific) in extracellular Ringer’s solution at 37°C and 5% CO2 ...
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bioRxiv - Neuroscience 2022Quote: ... Cortical astrocytes were plated at a density of 2.5 × 104 to 5 × 104 cells.cm−2 in Minimum Essential Media (Gibco, MEM) supplemented with 10% Horse Serum (Gibco) ...
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bioRxiv - Biochemistry 2022Quote: ... 2 µL of 20 µM siRNA and 5 µL RNAiMAX were diluted in 400 µL OptiMEM (Thermo Fisher Scientific), mixed gently and incubated for 20 min at room temperature ...
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bioRxiv - Molecular Biology 2022Quote: Cells were then counterstained with DAPI (4,6-diamidino-2-2phenylindole) in 5% BSA with 0.1% Triton X-100 and mounted with Prolong Gold Antifade mounting medium (Invitrogen). Images were then acquired with Olympus Fluoview FV1000 confocal microscope system with PLAPON 60X O SC NA 1.40 ...
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bioRxiv - Immunology 2022Quote: ... IgG was eluted from the resin in 5 × 2 mL fractions using IgG elution buffer (Pierce ThermoFisher, catalog #21004). Fractions were buffer exchanged into PBS ...
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bioRxiv - Cell Biology 2022Quote: Isolated colonic crypts or organoids were loaded at RT with Fura-2/AM (5 μm, 2h; Thermo Fisher Scientific) in HBS ...
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bioRxiv - Cell Biology 2022Quote: ... non-IR and IR animals were systemically injected with 0.9% saline containing 0.25 mg per 25 g of body weight of 5-ethynyl-2’-deoxyuridine (EdU, Thermofisher Scientific) followed by a 2 hour or 7 days chase before sacrifice ...
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bioRxiv - Microbiology 2022Quote: ... A total of 5×105 naïve HEp-2 cells were stained with Vybran DIO Cell-Labeling Solution (0.005 μg/ml, Thermo fisher) for 20 min at 37 °C and washed with PBS ...
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bioRxiv - Biophysics 2022Quote: ... Blocking was performed for 2 hours at room temperature with 1X PBS supplemented with 5% Fetal Bovine Serum (Invitrogen) and 0.25% Tween-20 (Invitrogen ...
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bioRxiv - Neuroscience 2021Quote: ... tissue was rinsed in PBS (2 × 5 min) and placed in a 9 mm wide × 0.5 mm deep gasket (Invitrogen) on a glass slide that was previously coated with Poly-L-Lysine (1 µl ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were plated at 1 x 10^6 cells per well of a 6 well plate or 5 x 10^6 cells per T25 flask in stage 2 media consisting of 47.5% IMDM (Gibco), 47.5% F12 (Gibco) ...
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bioRxiv - Microbiology 2021Quote: ... and an Acclaim PepMap 100 trap column (100 μm x 2 cm, nanoViper, C18, 5 μm, 100Å; Thermo Scientific), the tryptic peptides were separated by increasing concentrations of 80% ACN / 0.1% formic acid at a flow of 250 nl/min for 120 min and analyzed with a QExactive Plus mass spectrometer (Thermo Scientific) ...
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bioRxiv - Neuroscience 2021Quote: ... The culture medium was changed every 2 to 3 days and the cells were split every 5 to 7 days using 0.25% Trypsin-EDTA (Gibco), up to 20 times ...
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bioRxiv - Biochemistry 2021Quote: ... and an Acclaim PepMap 100 trap column (100 μm × 2 cm, nanoViper, C18, 5 μm, 100 Å; Thermo Scientific) were used to separate tryptic peptides by increasing concentrations of 80 % (v/v ...
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bioRxiv - Microbiology 2021Quote: ... and an Acclaim PepMap 100 trap column (100 μm x 2 cm, nanoViper, C18, 5 μm, 100Å; Thermo Scientific), the tryptic peptides were separated by increasing concentrations of 80% acetonitrile / 0.1% formic acid at a flow of 250 nL/min for 120 minutes and analyzed with a QExactive Plus mass spectrometer (Thermo Scientific ...
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bioRxiv - Microbiology 2020Quote: ... Samples of 5 μL were loaded onto a trapping column (Acclaim PepMap100 C18, 75 μm × 2 cm, Thermo Scientific) using 96% eluent A (0.1% formic acid ...