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Citations for Thermo Fisher :
2151 - 2200 of 10000+ citations for Human Procollagen II C Terminal Propeptide PIICP CLIA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2019Quote: ... 2002) containing the 35S Cauliflower Mosaic virus promoter and eGFP gene using the Gateway® LR Clonase™ II kit (Invitrogen Corp., Carlsbad, CA). A single colony was used to amplify the [Gene-pK7FW2/pK7WGF2] plasmid and it was sequenced before transforming Agrobacterium (strain GV3101 ...
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bioRxiv - Cell Biology 2021Quote: ... The NFE2L2 ORF was cloned into the pLenti-CMV-Puro vector using a Gateway LR Clonase II kit (Cat# 11791-020, Thermo Fisher Scientfic, Waltham, MA). The cytosolic GPX4 (cGPX4 ...
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bioRxiv - Cancer Biology 2020Quote: ... The MCL1 ORF was cloned into the pLenti CMV vector using a Gateway LR Clonase II kit (Cat# 11791-020, Thermo Fisher Scientfic, Waltham, MA). The resulting plasmid is missing a stop codon ...
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bioRxiv - Microbiology 2021Quote: Viral RNA in the apical wash was isolated using the Cells-to-cDNA II cell lysis buffer kit (Thermo Fisher Scientific, catalogue no. AM8723). Briefly ...
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bioRxiv - Developmental Biology 2020Quote: ... The 5’ and 3’ homology arm PCRs were cloned into pCR-Blunt II-TOPO using a Zero Blunt™ TOPO™ PCR Cloning Kit (Invitrogen, catalog #450245) to generate pJW1776 and pJW1781 ...
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bioRxiv - Cell Biology 2020Quote: ... eGFP mRNA was transcribed in vitro from a linearized pBluescript II SK+ construct using the T7 mMessage Ultra kit (Ambion/ThermoFisher Scientific, Waltham, MA, USA). All synthesized RNA was treated with DNase I (NEB ...
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bioRxiv - Microbiology 2021Quote: ... For hybridisation experiments radioactively labeled PCR probes against the selected targets were generated using DECAprime II DNA labeling kit and [α-32P]dCTP (Life Technologies, Thermo Fisher Scientific). Membranes were subsequently incubated with the labeled PCR fragments against the mRNA of the cluster HVO_B0192-HVO_B0193 and against the mRNA of HVO_0739.
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bioRxiv - Microbiology 2021Quote: ... For hybridisation experiments radioactively labeled PCR probes against the desired targets were generated using DECAprime II DNA labeling kit and [α-32P]dCTP (Life Technologies, Thermo Fisher Scientific). Membranes were subsequently incubated with the labeled PCR fragments.
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bioRxiv - Microbiology 2022Quote: Six libraries were constructed by first cloning DNA fragments into the pCR™Blunt II-TOPO™ vector using Zero Blunt™ TOPO™ PCR Cloning Kit (Invitrogen), followed by PCR amplification of the inserts and Gibson assembly into barcoded pNBU2_repA1 ...
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Kynurenic acid promotes A1 to A2 astrocyte conversion against HIV-associated neurocognitive disorderbioRxiv - Microbiology 2022Quote: ... cDNA was run for quantitative PCR using TB Green® Premix Ex Taq™ II (Tli RNaseH Plus) kit on QuantStudio 6 and 7 Flex real-time PCR systems (Thermo Fisher, USA). Reagents mentioned above were obtained from Takara (Japan) ...
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bioRxiv - Microbiology 2022Quote: ... The gene block was then moved into the pCR™-Blunt II-TOPO™ vector using the Zero Blunt™ TOPO™ PCR Cloning Kit (ThermoFisher) and using EcoRI and NheI to move the gene block into IFN-Beta_pGL3 ...
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bioRxiv - Molecular Biology 2024Quote: ... DNA was extracted for nanopore sequencing using the automated KingFisher Flex platform using the MagMax™ Viral/Pathogen II Nucleic Acid Isolation Kit (Thermo Fisher Scientific, UK). DNA was sequenced on an ONT GridION using the rapid barcoding kit (SQK-RBK004 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... the generated DNA fragment was purified from agarose gel and subcloned into a pCR™Blunt II-TOPO™ vector using Zero Blunt TOPO PCR cloning kit (ThermoFisher Scientific, USA). Afterwards ...
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bioRxiv - Plant Biology 2023Quote: ... and subse-quently ligated into the vector system pCR-Blunt II-TOPO via the Zero Blunt™ TOPO™ PCR cloning kit (Thermo Fisher Scientific). The lncRNA sequences were confirmed by Sanger chain-termination sequencing provided by eurofins Genomics (Ebersberg ...
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bioRxiv - Genomics 2024Quote: Total genomic DNA was extracted using Bioline ISOLATE II Plant DNA Kit and quantified using a Qubit fluorometer 2.0 (Thermo Fisher Scientific, Waltham, MA, USA). The RADseq library preparation followed the general steps from (Balao et al. ...
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bioRxiv - Cancer Biology 2022Quote: ... Antibodies used for human cells are mouse anti-human CD3 conjugated to APC (Thermo Fisher, 47-0036-42), mouse anti-human CD8 conjugated to FITC (BD Biosciences ...
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bioRxiv - Developmental Biology 2021Quote: ... where EGF and noggin were replaced with human forms with 10 ng ml−1 human EGF (Invitrogen, PHG0311), 100 ng ml−1 human noggin (Peprotech ...
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bioRxiv - Cell Biology 2022Quote: Human cerebral organoids are generated from human induced pluripotent stem cells (iPSCs) which were purchased from GibcoTM (Gibco by Thermofisher ...
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bioRxiv - Cancer Biology 2020Quote: ... anti-human TNF-α (hTNF-α)-PE and anti-human IFN-ɣ (hIFN-ɣ)-PECy7 were from ThermoFisher. Anti-human CD4 (hCD4)-BV711 was from Biolegend.
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bioRxiv - Immunology 2020Quote: ... using a secondary antibody recognizing all human immunoglobulin isotypes (goat anti-human IgG H+L-HRP, Invitrogen/ThermoFisher) [12,13] ...
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bioRxiv - Immunology 2020Quote: ... mouse anti-human IgG3 (HP6050) or mouse anti-human IgM (clone HP6083) at 1/1000 (Thermo Fisher Scientific) for 1 hour at RT ...
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bioRxiv - Immunology 2021Quote: ... which contains the gene expression profile of human microglia using a human gene ST 2.0 Microarray Chip (Affymetrix). The human microglia were polarized to either pro-inflammatory (M1 ...
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bioRxiv - Immunology 2020Quote: ... using a secondary antibody recognizing all human immunoglobulin isotypes (goat anti-human IgG H+L-HRP, Invitrogen/ThermoFisher) [12,13] ...
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bioRxiv - Neuroscience 2024Quote: ... The concentrations of human α-synuclein were measured using a human α-syn-specific ELISA (ThermoFisher Scientific #KHB0061) according to the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2024Quote: ... Human full-coding KATNA1 cDNA was isolated from a human fetal brain Marathon-ready cDNA collection (Clontech, Invitrogen) with the following forward and reverse primers ...
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bioRxiv - Bioengineering 2023Quote: ... primary human T cells were thawed and activated with anti-CD3/CD28 Human T-Expander Dynabeads (Thermo Fisher) at a 3:1 bead to cell ratio ...
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bioRxiv - Synthetic Biology 2023Quote: ... primary human T cells were thawed and activated with anti-CD3/CD28 Human T-Expander Dynabeads (Thermo Fisher) at a 3:1 bead to cell ratio ...
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bioRxiv - Immunology 2024Quote: ... mouse anti-human IgG3 (HP6050) or mouse anti-human IgM (clone HP6083) at 1/1000 (Thermo Fisher Scientific) for 1 hour at room temperature ...
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bioRxiv - Immunology 2021Quote: ... and on the surface of H1568 EVs (with IFN-γ stimulation) were quantified using a PD-L1 Human ELISA kit (#BMS2212, ThermoFisher Scientific). For the cells ...
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bioRxiv - Neuroscience 2021Quote: ... cRNA transcripts encoding human KCNQ1-5 were generated by in vitro transcription using the T7 polymerase mMessage mMachine kit (Thermo Fisher Scientific) or SP6 polymerase mMessage mMachine kit (Thermo Fisher Scientific) ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Colorimetric protein assays were conducted using commercial human IL-8 ELISA and mouse IL-6 ELISA kits (Invitrogen; 88-8086, 88-7064) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2019Quote: Culture medium from 7 div OBSCs was assayed for human Aβ1-40 and Aβ1-42 using commercially available ELISA kits (Life Technologies: KHB3441 and KHB3481). Briefly ...
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bioRxiv - Biochemistry 2022Quote: ... Resting CD4+ T cells were purified from bulk PBMCs by using Dynabeads™ Untouched™ Human CD4 T Cells Kit (ThermoFisher Scientific).
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bioRxiv - Bioengineering 2020Quote: ... Primary human T cells were isolated from PBMCs using Pan T Cell Isolation Kit (Miltenyi, 130-096-535) and activated with Dynabeads® Human T-Expander CD3/CD28 (Gibco, 11141D). Three days later ...
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bioRxiv - Synthetic Biology 2021Quote: ... Supernatant was harvested at the indicated timepoints and IL-2 levels in the supernatant were measured via IL-2 Human Instant ELISA kit (Thermo Fisher #BMS221INST). T-cell proliferation was also measured at the indicated timepoints using a BD FACSymphony Fortessa X-50.
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bioRxiv - Biochemistry 2021Quote: Resting CD4+ T cells were purified from bulk PBMCs by using Dynabeads™ Untouched™ Human CD4 T Cells Kit (ThermoFisher Scientific). Selected CD4+ T cells were activated by Dynabeads™ Human T-Activator CD3/CD28 kit (ThermoFisher Scientific ...
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bioRxiv - Cancer Biology 2022Quote: ... These plasma samples were diluted at least 2x or more (as required) and then were used to detect PTN by ELISA using commercial human PTN ELISA kits (ThermoFisher Scientific, EH370RB). CXCL5 ELISA from tumor lysates was performed using commercial kit (R&D Systems ...
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bioRxiv - Genetics 2020Quote: RNA was extracted from pulverized left or right ventricle human cardiac tissue using the mixVana miRNA isolation kit (Thermo Fisher Scientific, #AM1560), according to the manufacturer’s specifications ...
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bioRxiv - Immunology 2021Quote: ... Insertion of single base pair changes for the generation of human and mouse FOXN1 variants was achieved by site-directed mutagenesis using the Phusion site-directed mutagenesis kit (Thermo Fisher Scientific).Specific primers for the introduction of single base pair deletions or single base pair changes were designed using the following software www.agilent.com/store/primerDesignProgram.jsp ...
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bioRxiv - Cell Biology 2022Quote: ... media was collected at the end of 48 h of lipid loading and albumin concentration was measured with a commercially available human albumin ELISA kit (Invitrogen, Waltham, MA).
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bioRxiv - Immunology 2022Quote: ... restimulated as well as an unstimulated Mel15-PBMC sample from the same timepoint using the Dynabeads™ Untouched™ Human CD8 T Cells Kit (Invitrogen). Single ...
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bioRxiv - Evolutionary Biology 2024Quote: ... from Illumina and the Axiom™ Genome-Wide Human Origins (HO) and the Axiom™ Precision Medicine Diversity Array Plus Kit (PMDA) from ThermoFisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... the first-strand human cDNA was obtained using oligo(dT) primers and Moloney murine leukemia virus-reverse transcriptase (MMLV-RT kit; Life Technologies, USA), and The SuperScript™ VILO™ cDNA Synthesis Kit ...
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bioRxiv - Neuroscience 2023Quote: ... Samples were then measured by using human Aβ1-42 enzyme-linked immunoassay (ELISA) kit (Catalog No. KHB3441, Invitrogen by Thermo Fisher Scientific).
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bioRxiv - Microbiology 2023Quote: Levels of IFN-λ in the culture supernatants were determined with ELISA using the human IL-29 ELISA kit (Invitrogen, cat. BMS2049) following the instructions of the manufacturers ...
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bioRxiv - Cancer Biology 2023Quote: ... These tissues were stained with Abs against human CD4 pre-labeled with Alexa Fluor 488 using an Ab labeling kit (Molecular Probes, A20187), human/mouse Foxp3 (clone 1054c ...
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bioRxiv - Microbiology 2023Quote: ... The level of IL-8 in cell culture supernatants was determined using a Human IL-8 Uncoated ELISA kit (Invitrogen, ThermoFisher Scientific Inc.).
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bioRxiv - Cancer Biology 2023Quote: ... the sections were stained with an anti-human CD4 Ab that was pre-labeled with Alexa Fluor 488 using an Ab labeling kit (Molecular Probes, A20187), anti-human/mouse Foxp3 Ab pre-labeled with Alexa Fluor 555 (Molecular Probes ...
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bioRxiv - Physiology 2023Quote: ... 0.5-1 µg of total RNA was isolated from human islets and reverse transcribed using an M-MLV RT kit (Invitrogen, MA, USA). TaqMan primers (Applied Biosystems ...
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bioRxiv - Cell Biology 2023Quote: ... and C-X-C motif chemokine 11 (CXCL11) ELISAs were performed using basal supernatants collected 48 hours after cytokine treatments using IL-8 Human ELISA Kit (ThermoFisher, Cat# KHC0081) and Human CXCL11/I-TAC Quantikine ELISA Kit (R&D Systems ...