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Citations for Thermo Fisher :
1951 - 2000 of 10000+ citations for Human Procollagen II C Terminal Propeptide PIICP CLIA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... Fresh primary human T cells were isolated and cultured with anti-human CD3/CD28 beads (Dynabeads, ThermoFisher), 500U/mL IL-2 (Proleukin ...
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bioRxiv - Molecular Biology 2021Quote: Human adipose-derived stem cells (ASCs) and human microvascular endothelial cells (HMVEC) were purchased from Thermo Fisher Scientific (Carlsbad ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: Primary human epidermal melanocytes (MC) and primary human epidermal keratinocytes (KC) were obtained from Invitrogen (NY, USA). MC was cultured in Medium
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bioRxiv - Biochemistry 2023Quote: ... 1.0 mg/mL recombinant human insulin, 0.55 mg/mL human transferrin, 0.67 μg/mL sodium selenite, Gibco), and 0.04 μg/mL dexamethasone (Sigma Aldrich) ...
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bioRxiv - Neuroscience 2023Quote: ... Human and non-human primate OBs were sectioned following embedding in optimal cutting temperature (O.C.T.) compound (ThermoFisher). One human non-synucleinopathy case was obtained from banner health embedded in paraffin wax (Specimen details see Table 1 and SI Appendix Table S1) ...
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bioRxiv - Immunology 2023Quote: Primary human T cells were pre-activated with DynabeadsTM human T-Activator CD3/CD28 beads (Gibco, #11131D) at a 1:1 ratio of cells:beads with 100 IU/ml IL-2 (Chiron #53905-991-01 ...
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bioRxiv - Neuroscience 2023Quote: ... Human fibroblasts and Human embryonic kidney (HEK) 293T cells were cultured in DMEM (1g/L glucose) (GIBCO) supplemented with 10% Fetal Calf Serum (FCS) ...
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bioRxiv - Molecular Biology 2021Quote: ... the isolated RNA samples were reverse transcribed to cDNA using superscript II (Transcriptase inverse SuperScript™ II, Invitrogen™ Cat#18064022) following manufacturer’s instruction ...
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bioRxiv - Biophysics 2020Quote: ... Myosin II filaments are prepolymerized by adding monomeric myosin II (20 nM, chicken skeletal muscle, fluorescently labeled with Alexa 647, Life Technologies) to an actin free sample solution and allowing to incubate for 10 min.
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bioRxiv - Physiology 2019Quote: ... was digested in Collagenase II and DNase 1 (600 U/ml Collagenase II; CLS-2 Worthington, 60 U/ml DNAse 1; Ambion, Warrington) in Hank’s Balanced Salt Solution (HBSS ...
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bioRxiv - Cancer Biology 2021Quote: ... The PCR product was cloned using Gateway BP Clonase II Enzyme mix and Gateway LR Clonase II Enyzme Mix (ThermoFisher Scientific) via donor vector pDonR211 into the final Doxycycline inducible expression vector pInducer20 [20] according to manufacturer’s instructions ...
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bioRxiv - Plant Biology 2019Quote: ... the pENTR/D PPC1 RNAi clone was recombined into intron-containing hairpin RNAi binary vector pK7GWIWG2(II) (Karimi et al., 2002) using LR Clonase II enzyme mix (Life Technologies). Agrobacterium-mediated stable transformation of K ...
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bioRxiv - Biophysics 2020Quote: Madin Darby canine kidney II (MDCK-II) (ECACC, Cat. No. 00062107) and MDCK Myr-Palm-GFP H2B-mCherry cells were cultured in DMEM (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2022Quote: ... chloride hemipentahydrate (Cd2+), lead (II) nitrate (Pb2+), mercury (II) chloride (Hg2+), and hydrogen peroxide (H2O2, 30% in water) were purchased from Fisher Scientific. Buthionine sulfoximine (BSO) ...
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bioRxiv - Genetics 2021Quote: The coding sequence for the pH insensitive Citrine fluorescent protein was inserted in the coding sequence of the BK2L3 protein (Zm00001d034049) immediately after the N-terminal secretory peptide domain and synthesized in the pDONOR221 vector (Life Technologies, USA) (Tian et al. ...
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bioRxiv - Cell Biology 2020Quote: S1R was expressed with an N-terminal 6*His-tag fusion in Sf9 cells using Bac-to-Bac baculoviral expression system (Thermo Fisher Scientific) according to manufacturer’s recommendations ...
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bioRxiv - Neuroscience 2019Quote: To isolate prefrontal cortex synaptic terminals synaptosomes were freshly prepared using Syn-PER™ Synaptic Protein Extraction Reagent (catalog no. 87793, ThermoFisher Scientific) exactly as recommended by the manufacturer ...
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bioRxiv - Plant Biology 2021Quote: ... Final constructs were obtained by transferring all candidate genes subsequently into the plant binary destination vector pAlligator2 (N-terminal triple HA fusions=HA3) (Bensmihen et al., 2004) via LR reactions (Thermo Fisher Scientific). Constructs were transformed into Agrobacteria (Rhizobium radiobacter ...
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bioRxiv - Biophysics 2021Quote: ... was cloned into a modified pEZT-BM vector with an N-terminal Flag tag (Morales-Perez et al., 2016) and heterologously expressed in HEK293F cells using the BacMam system (Thermo Fisher Scientific). Bacmids were synthesized using E ...
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bioRxiv - Biophysics 2022Quote: ... and apoA1 were purified using established protocols for MSP44 with the following modifications: A synthetic construct of apoA1 bearing a 3C protease cleavable N-terminal deca-histidine tag (GeneArt/Thermo Fisher Scientific) was cloned into a pET28a vector (Addgene ...
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bioRxiv - Biophysics 2022Quote: ... a pET21a vector including hTS with an N-terminal 6x histidine tag was transformed into BL21 (DE3) cells lacking the rne131 gene (Invitrogen, Carlsbad, CA). After an adaptation period (Bonin & Lee ...
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bioRxiv - Biophysics 2022Quote: The gene encoding N-terminal His-tagged V2HeR3 was cloned into the EcoRI and XbaI site of pPICZB vector (Thermo Fisher Scientific). The recombinant protein was expressed in the Pichia pastoris strain SMD1168H (Philosof et al. ...
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bioRxiv - Biophysics 2022Quote: ... the gene encoding N-terminal His-tagged V2HeR3 was cloned into the EcoRI and XbaI site of pPICZB vector (Thermo Fisher Scientific). For determination of λmax of V2HeR3 WT and mutants ...
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bioRxiv - Immunology 2020Quote: ... FcMBL was biotinylated at the N terminus of the Fc protein using an N-terminal amino-oxy reaction and then coupled to streptavidin superparamagnetic beads (1µM MyOne Dynabead (Thermo Fisher Scientific, USA). Samples were screened using 5 µg of the FcMBL beads ...
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bioRxiv - Microbiology 2021Quote: ... with C-terminal HRV3C-cleavable 8xHis tag) or SARS-CoV-2 RBD (residue 319-537) were transfected into Expi293F GnTI-Cells (Thermo Fisher Scientific) using PEI ...
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bioRxiv - Cell Biology 2022Quote: ... transgenes were recombined with the Drosophila Gateway Vector Collection (DGVC) destination vectors expressing the N terminal tag fused target proteins under Actin5C promoter (Invitrogen, Carlsbad, CA). In addition ...
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bioRxiv - Cancer Biology 2020Quote: ... with C-terminal twin-Strep-10x His dual tag followed by a stop codon was synthesized using GeneArt Gene Synthesis service (Invitrogen, Carlsbad, CA) with SgfI and MluI restriction sites at the beginning and the end of the ORF sequence ...
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bioRxiv - Developmental Biology 2020Quote: N-terminal fusions of MtHDTs with GFP under the control of their own promoters were constructed using MultiSite Gateway Technology (Thermo Fisher Scientific). The coding sequence (CDS ...
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bioRxiv - Developmental Biology 2021Quote: ... control and morphant embryos was carried out as described in [77] with the following reagents: terminal deoxynucleotidyl transferase (TdT) (15 U/μl and 5x buffer, Thermofisher Ref. 10533065), digoxigenin-11-dUTP (25 nmol/25μl ...
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bioRxiv - Molecular Biology 2021Quote: ... Genes were cloned into modified pRSF1-Duet expression vector with a TEV-cleavable N-terminal 14-histidine tag (39) using New England Biolabs and Anza (Thermo Fisher Scientific) enzymes ...
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bioRxiv - Biochemistry 2022Quote: ... The flow-through was ethanol precipitated and the cDNA pellet was dissolved in 19 μl of Terminal deoxynucleotidyl transferase (TdT) tailing mixture containing 1x TdT buffer (Thermo Fisher Scientific) and CTP (1.25 mM) ...
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bioRxiv - Biochemistry 2022Quote: N-terminal amino acid sequencing for the eOD-GT8 60mer was performed using the Procise Protein Sequencing System (Applied Biosystems, CA, USA). The protein band corresponding to the eOD-GT8 60mer (~50 kDa ...
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bioRxiv - Microbiology 2023Quote: ... Blood was collected by terminal cardiac puncture immediately after CO2 asphyxiation and transferred to serum gel blood tubes (Thermo Fisher Scientific, MA). After a minimum of 20 minutes at room temperature ...
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bioRxiv - Biochemistry 2023Quote: ... Full-length genes of human RNGTT and CMTR1 were cloned into pMlink vector containing a 4×ProteinA tag at the N-terminal and were transfected to Expi293F cells (Thermo Fisher Scientific) when cell density is 2.5× 106 /ml ...
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bioRxiv - Cancer Biology 2023Quote: Wild-type and mutant (C139A) USP39 with a Flag-tag at its N-terminal were cloned into the pLenti6/V5 lentiviral vector (Invitrogen, California, USA). Specific shRNA oligonucleotides targeting USP39 were cloned into the pLL3.7 lentiviral vector (Addgene) ...
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bioRxiv - Biochemistry 2024Quote: ... hamster (Syrian Hamster Isoform X1 – UniProt: A0A1U8BWQ2) and macaque (Macaque – UniProt: F6SVR2) with an N-terminal cMYC-tag were ordered to GeneArt (Thermo Fisher Scientific) and cloned into a phCMV backbone (GeneBank ...
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bioRxiv - Biochemistry 2024Quote: ... with a replacement in the N-terminal domain loop 8 (139CQKRDGPH146 to 139AEAG142, designated as rA3GR8) was constructed in the pET-sumo vector (Thermo Fisher Scientific). This construct generated a SUMO fusion with an N-terminal 6xHis tag and a PreScission protease cleavage site ...
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bioRxiv - Immunology 2021Quote: ... 4°C) and incubated overnight at 4°C with gentle rotation with 40 μl Pierce Anti-c-Myc Magnetic Beads (ThermoFisher Scientific Cat# 88842) or anti-Flag® M2 Magnetic Beads (Merck Cat# M8823 ...
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bioRxiv - Neuroscience 2022Quote: ... sh-c-Jun and c-Jun over-expressed (pCMV-c-Jun) plasmid were transfected into cells using lipofectamine 3000 reagent (Invitrogen; Carlsbad, CA, USA) according to the manufacturer’s recommendation ...
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bioRxiv - Systems Biology 2021Quote: ... The Feed C regime used commercial CD EfficientFeed™ C AGT™ (Invitrogen, UK), whereas the U and U40 feeds aimed to provide growth-limiting nutrients (glucose and amino acids ...
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bioRxiv - Molecular Biology 2021Quote: ... Add 100 µL Pol II antibody-conjugated beads (10 µg Pol II 8WG16 antibody conjugated with Dynabeads™ Protein G for Immunoprecipitation (ThermoFisher, #1004D)) and incubate on a tube rotator for 1 hour in the 4 °C room ...
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Single-cell analysis of skeletal muscle macrophages reveals age-associated functional subpopulationsbioRxiv - Cell Biology 2022Quote: ... Pellet 1 was subjected to a second round of digestion in 1 mL of 1000 U/mL Collagenase type II and 1 mL of 11 U/mL Dispase II (Thermofisher, Cat# 17105041) along with the 8 mL of the remaining cell suspension ...
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Bidirectional neuronal migration coordinates retinal morphogenesis by preventing spatial competitionbioRxiv - Developmental Biology 2021Quote: ... zebrafish embryos were incubated at 4°C for 1 h in E3 supplemented with 500 μm of EdU (ClickiT-Alexa 488 fluorophore kit, Invitrogen) while human retinal organoids were incubated at 37°C for 1h in organoid medium supplemented with 10 μm of EdU ...
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bioRxiv - Molecular Biology 2020Quote: ... was incorporated into cells on glass coverslips during 15 min at 37°C and revealed using the Click-It EdU Alexa Fluor 647 Imaging kit (Invitrogen) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... reverse-transcribed at 50°C for 1 h ourwith SuperScript III and 1 mM of each dNTP (SuperScript III cDNA synthesis kit, Invitrogen) followed by 18-cycle PCR amplification.
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bioRxiv - Molecular Biology 2021Quote: ... and frozen at −80°C until DNA extraction with a magnetic-based method (MagMAX CORE Nucleic Acid Purification Kit – ThermoFisher). Quantitative PCR reactions and data analysis were performed as described above ...
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bioRxiv - Molecular Biology 2021Quote: J1 mES cells were grown on glass coverslips and incubated for 1h (37°C, 5% CO2) with EU from the Click-iT RNA Alexa Fluor 594 imaging kit (C10330, Invitrogen). Cells were fixed with 3,7% formaldehyde in PBS 1× for 15min at room temperature and permeabilized with 0.5% Triton X-100 in PBS 1× for 15min at room temperature ...
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bioRxiv - Molecular Biology 2021Quote: ... in RNAse HI buffer for 30 min at 37°C and then nucleic acids were purified with GeneJET Gel Extraction and DNA cleanup micro kit (General cleanup protocol, ThermoFisher). IP for treated aliquot using S9.6 antibodies and nucleic acid purification were performed as described for the untreated aliquot.
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bioRxiv - Pathology 2020Quote: ... The samples were kept at -80°C until protein extraction using the PARIS™ kit (Thermo Fisher, Waltham, MA, USA). Protein concentration was quantified by BCA assay (Thermo Fisher) ...
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bioRxiv - Cell Biology 2021Quote: ... TDP-43 CRISPR KO HeLa cells were lysed for N/C fractionation and SDS-PAGE with the NE-PER kit (ThermoFisher) according to the manufacturer’s instructions ...