Labshake search
Citations for Thermo Fisher :
2001 - 2050 of 10000+ citations for Human Procollagen II C Terminal Propeptide PIICP CLIA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... The 5 µg of Std-OmRV (508-684)/pMA plasmid was linearised with an overnight digestion of XhoI at 37°C and was then transcribed to RNA using mMESSAGE mMACHINE High-Yield Capped RNA Transcription SP6 Kit (Invitrogen) for the following RT-qPCR ...
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bioRxiv - Molecular Biology 2022Quote: ... The membrane was washed 3 x for 15 minutes with 50 ml of pre-warmed 2 x SSC buffer containing 0.5 % SDS at 37°C and the biotin signal was detected with Chemiluminiscent Nucleic Acid Detection Module kit (ThermoFisher Scientific) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... Cell debris were pelleted by centrifugation (14,000g, 10min, 4°C) and whole cell protein extract concentration was measured using Qubit Protein Assay Kit (Thermo Fisher). Protein extracts were immediately used for EnzyChrom Catalase Assay Kit and EnzyChrom Glutathione Reductase Assay Kit (BioAssay Systems) ...
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bioRxiv - Biochemistry 2022Quote: ... Lysates were clarified by centrifugation at 20,800 g at 4°C for 10 min and supernatant protein concentrations quantified using the Bradford assay kit (ThermoFisher, #23236)
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bioRxiv - Microbiology 2019Quote: ... prepared minipreps from 2 ml of O/N cultivation at 37 °C using the GeneJET Plasmid Miniprep Kit (ThermoFisher Scientific), checked for the presence of insert using restriction digest and agarose gel electrophoresis ...
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bioRxiv - Microbiology 2021Quote: ... the transcription process was performed at 37°C overnight using the T7 High Yield Transcription Kit (Thermo Fisher Scientific, USA). Finally ...
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bioRxiv - Biochemistry 2021Quote: ... Samples were subjected to centrifugation at 14,000 × g and the supernatant was stored at −80 °C or collected for protein quantification using a BCA assay kit according to the manufacturer’s instructions (ThermoFisher 23227). Equal volumes of whole-cell protein extract were subjected to electrophoresis through denaturing 10% bis-Tris polyacrylamide gels and transferred to PVDF membrane ...
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bioRxiv - Synthetic Biology 2021Quote: ... Cells were isolated by centrifugation (4,000 × g at 4°C for 15 min) and plasmid DNA isolated using a GeneJet mini-prep kit (ThermoFisher, UK) following manufacturer’s recommendations ...
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bioRxiv - Neuroscience 2021Quote: ... and p.Arg288Ter iPSCs (from ST3GAL5 c.862C>T (p.Arg288Ter) patient fibroblasts) was performed using the CytoTune-iPS Sendai Reprogramming Kit (Invitrogen, # A13780) and the CytoTune-iPS 2.0 Sendai Reprogramming Kit (ThermoFisher ...
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bioRxiv - Immunology 2020Quote: ... supernatants were discarded and cells were frozen at -80°C before addition of reagents from the CyQUANT Cell Proliferation Assay kit (ThermoFisher) as indicated by the manufacturer.
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bioRxiv - Microbiology 2020Quote: ... Cells were then grown overnight in a 50 mL liquid culture at 33°C and DNA purified using the PureLink HiPure plasmid midiprep kit (Invitrogen). Transformation efficiency was estimated by plating serial dilutions of the transformation on agar plates ...
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bioRxiv - Immunology 2021Quote: ... following the manufacturer’s protocol and complementary (c)DNA synthesized using the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems™) per the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... These isolates were grown in LB broth overnight at 37°C and genomic DNA was extracted using the PureLink Genomic DNA Mini kit (Invitrogen). Illumina sequencing was performed using Nextera XT DNA Library Preparation Kit and Miseq System yielding 251-bp paired-end reads ...
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bioRxiv - Molecular Biology 2023Quote: ... Lysates were centrifuged at 16000g for 20 min at 4°C and Pierce BCA Protein Assay Kit (Thermo Fisher Scientific) was used to determine cleared lysate concentration ...
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bioRxiv - Developmental Biology 2022Quote: ... 4°C and supernatant was collected and the protein content was quantified with a BCA kit (Thermo Fisher Scientific, 23225). Equal amount of protein was denatured by mixing with 4x Laemmli buffer (Bio-Rad ...
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bioRxiv - Developmental Biology 2022Quote: ... 48 hpf embryos were incubated at 4°C for 1 h in E3 supplemented with 500 μm of EdU (ClickiT-Alexa 488 fluorophore kit, Invitrogen) in 10% DMSO ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... 4 °C) and the total protein concentration determined by BCA protein assay (Pierce BCA Protein Assay Kit, Thermo Scientific, 23225) following manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2023Quote: ... in −20°C until total RNA was extracted using the PureLink RNA Mini Kit (Thermo Fisher Scientific; Cat no. 12183018A) according to the manufacturer’s instructions and quantified using NanoDrop (Thermo Scientific) ...
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bioRxiv - Molecular Biology 2023Quote: ... The purified RNA (1 μg) was converted to cDNA by random hexamers using Verso c-DNA Kit (Thermo Fisher Scientific) following manufacturers’ protocol ...
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bioRxiv - Developmental Biology 2023Quote: ... 5‘-AAA AAA AGC ACC GAC TCG GTG CCA C-3’) and in vitro transcribe using the MAXIscript T7 kit (Invitrogen) and purified with a miRNeasy mini kit (QIAGEN ...
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bioRxiv - Immunology 2024Quote: ... Full differentiation into mature MCs was confirmed by expression of lineage-specific c-KIT (rat anti-CD117 FITC-conjugated, 1:200, Invitrogen) and FcεRI (armenian hamster anti-FcεRIα Alexa Fluor 647-conjugated ...
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bioRxiv - Bioengineering 2023Quote: ... The sample’s DNA was extracted using phenol-chloroform and kept at −20°C until applying the PicoGreen dsDNA assay kit (Invitrogen). Total sGAGs of the samples were obtained via papain (Sigma-Aldrich ...
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bioRxiv - Systems Biology 2024Quote: DNA for SureSelect assays and SNP arrays was purified from fresh frozen samples stored at -80°C using a PureLink Genomic DNA Kit (Invitrogen) according to the manufacturer’s instructions for mammalian tissue ...
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bioRxiv - Microbiology 2024Quote: ... The final purified DNA was stored at -80 °C and the DNA concentration was determined using Qubit HS Assay Kit (Invitrogen) on the Qubit 4 Fluorometric Quantification device (Invitrogen) ...
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bioRxiv - Cancer Biology 2022Quote: ... Antibodies used for human cells are mouse anti-human CD3 conjugated to APC (Thermo Fisher, 47-0036-42), mouse anti-human CD8 conjugated to FITC (BD Biosciences ...
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bioRxiv - Developmental Biology 2021Quote: ... where EGF and noggin were replaced with human forms with 10 ng ml−1 human EGF (Invitrogen, PHG0311), 100 ng ml−1 human noggin (Peprotech ...
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bioRxiv - Cell Biology 2022Quote: Human cerebral organoids are generated from human induced pluripotent stem cells (iPSCs) which were purchased from GibcoTM (Gibco by Thermofisher ...
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bioRxiv - Cancer Biology 2020Quote: ... anti-human TNF-α (hTNF-α)-PE and anti-human IFN-ɣ (hIFN-ɣ)-PECy7 were from ThermoFisher. Anti-human CD4 (hCD4)-BV711 was from Biolegend.
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bioRxiv - Immunology 2020Quote: ... using a secondary antibody recognizing all human immunoglobulin isotypes (goat anti-human IgG H+L-HRP, Invitrogen/ThermoFisher) [12,13] ...
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bioRxiv - Immunology 2020Quote: ... mouse anti-human IgG3 (HP6050) or mouse anti-human IgM (clone HP6083) at 1/1000 (Thermo Fisher Scientific) for 1 hour at RT ...
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bioRxiv - Immunology 2021Quote: ... which contains the gene expression profile of human microglia using a human gene ST 2.0 Microarray Chip (Affymetrix). The human microglia were polarized to either pro-inflammatory (M1 ...
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bioRxiv - Immunology 2020Quote: ... using a secondary antibody recognizing all human immunoglobulin isotypes (goat anti-human IgG H+L-HRP, Invitrogen/ThermoFisher) [12,13] ...
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bioRxiv - Bioengineering 2023Quote: ... primary human T cells were thawed and activated with anti-CD3/CD28 Human T-Expander Dynabeads (Thermo Fisher) at a 3:1 bead to cell ratio ...
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bioRxiv - Synthetic Biology 2023Quote: ... primary human T cells were thawed and activated with anti-CD3/CD28 Human T-Expander Dynabeads (Thermo Fisher) at a 3:1 bead to cell ratio ...
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bioRxiv - Neuroscience 2024Quote: ... The concentrations of human α-synuclein were measured using a human α-syn-specific ELISA (ThermoFisher Scientific #KHB0061) according to the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2024Quote: ... Human full-coding KATNA1 cDNA was isolated from a human fetal brain Marathon-ready cDNA collection (Clontech, Invitrogen) with the following forward and reverse primers ...
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bioRxiv - Biophysics 2021Quote: ... PCR products were either cloned into the pCR II-Blunt-TOPO vector using the Zero Blunt® TOPO® PCR cloning kit (ThermoFisher Scientific #K2875J10) for subsequent sequencing or sequenced directly ...
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bioRxiv - Plant Biology 2019Quote: ... 2002) containing the 35S Cauliflower Mosaic virus promoter and eGFP gene using the Gateway® LR Clonase™ II kit (Invitrogen Corp., Carlsbad, CA). A single colony was used to amplify the [Gene-pK7FW2/pK7WGF2] plasmid and it was sequenced before transforming Agrobacterium (strain GV3101 ...
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bioRxiv - Cell Biology 2021Quote: ... The NFE2L2 ORF was cloned into the pLenti-CMV-Puro vector using a Gateway LR Clonase II kit (Cat# 11791-020, Thermo Fisher Scientfic, Waltham, MA). The cytosolic GPX4 (cGPX4 ...
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bioRxiv - Cancer Biology 2020Quote: ... The MCL1 ORF was cloned into the pLenti CMV vector using a Gateway LR Clonase II kit (Cat# 11791-020, Thermo Fisher Scientfic, Waltham, MA). The resulting plasmid is missing a stop codon ...
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bioRxiv - Microbiology 2021Quote: Viral RNA in the apical wash was isolated using the Cells-to-cDNA II cell lysis buffer kit (Thermo Fisher Scientific, catalogue no. AM8723). Briefly ...
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bioRxiv - Developmental Biology 2020Quote: ... The 5’ and 3’ homology arm PCRs were cloned into pCR-Blunt II-TOPO using a Zero Blunt™ TOPO™ PCR Cloning Kit (Invitrogen, catalog #450245) to generate pJW1776 and pJW1781 ...
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bioRxiv - Cell Biology 2020Quote: ... eGFP mRNA was transcribed in vitro from a linearized pBluescript II SK+ construct using the T7 mMessage Ultra kit (Ambion/ThermoFisher Scientific, Waltham, MA, USA). All synthesized RNA was treated with DNase I (NEB ...
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bioRxiv - Microbiology 2021Quote: ... For hybridisation experiments radioactively labeled PCR probes against the selected targets were generated using DECAprime II DNA labeling kit and [α-32P]dCTP (Life Technologies, Thermo Fisher Scientific). Membranes were subsequently incubated with the labeled PCR fragments against the mRNA of the cluster HVO_B0192-HVO_B0193 and against the mRNA of HVO_0739.
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bioRxiv - Microbiology 2021Quote: ... For hybridisation experiments radioactively labeled PCR probes against the desired targets were generated using DECAprime II DNA labeling kit and [α-32P]dCTP (Life Technologies, Thermo Fisher Scientific). Membranes were subsequently incubated with the labeled PCR fragments.
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bioRxiv - Microbiology 2022Quote: Six libraries were constructed by first cloning DNA fragments into the pCR™Blunt II-TOPO™ vector using Zero Blunt™ TOPO™ PCR Cloning Kit (Invitrogen), followed by PCR amplification of the inserts and Gibson assembly into barcoded pNBU2_repA1 ...
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Kynurenic acid promotes A1 to A2 astrocyte conversion against HIV-associated neurocognitive disorderbioRxiv - Microbiology 2022Quote: ... cDNA was run for quantitative PCR using TB Green® Premix Ex Taq™ II (Tli RNaseH Plus) kit on QuantStudio 6 and 7 Flex real-time PCR systems (Thermo Fisher, USA). Reagents mentioned above were obtained from Takara (Japan) ...
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bioRxiv - Microbiology 2022Quote: ... The gene block was then moved into the pCR™-Blunt II-TOPO™ vector using the Zero Blunt™ TOPO™ PCR Cloning Kit (ThermoFisher) and using EcoRI and NheI to move the gene block into IFN-Beta_pGL3 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... the generated DNA fragment was purified from agarose gel and subcloned into a pCR™Blunt II-TOPO™ vector using Zero Blunt TOPO PCR cloning kit (ThermoFisher Scientific, USA). Afterwards ...
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bioRxiv - Plant Biology 2023Quote: ... and subse-quently ligated into the vector system pCR-Blunt II-TOPO via the Zero Blunt™ TOPO™ PCR cloning kit (Thermo Fisher Scientific). The lncRNA sequences were confirmed by Sanger chain-termination sequencing provided by eurofins Genomics (Ebersberg ...