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Citations for Thermo Fisher :
2151 - 2200 of 10000+ citations for 6 Propyl 2 thioxo 2 3 dihydropyrimidin 4 1H one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... and incubated for 2 h at 4°C with 50 µl of IgG magnetic PanMouse Dynabeads (Thermo Fisher) equilibrated with IP buffer ...
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bioRxiv - Molecular Biology 2023Quote: ... were blocked for 2 hr at 4 °C in lysis buffer containing 1 mg/ml yeast tRNA (Ambion) and washed twice with 1 ml lysis buffer ...
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bioRxiv - Cancer Biology 2023Quote: ... and 5% 2-mercaptoethanol] and was resolved by SDS-PAGE on 4% to 12% Bis-Tris gels (Invitrogen). Proteins were transferred to a polyvinylidene difluoride immobilon TM-P membrane (Millipore ...
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bioRxiv - Immunology 2023Quote: ... Expression of GOI protein (Figure 2) was determined by SDS–PAGE using 4–12% gradient polyacrylamide gels (Invitrogen) and Coomassie staining and by Western blotting using antigen-specific sera.
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bioRxiv - Cell Biology 2022Quote: ... equipped with a Dionex IonPac AG11-HC guard column (2 mm × 50 mm, 4 μm, Thermo Fisher Scientific). The column temperature was held at 30 °C ...
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bioRxiv - Cell Biology 2022Quote: ... onto a Dionex IonPac AS11-HC column (2 mm × 250 mm, 4 μm particle size, Thermo Fisher Scientific) equipped with a Dionex IonPac AG11-HC guard column (2 mm × 50 mm ...
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bioRxiv - Neuroscience 2023Quote: ... NPC were passaged every 2-4 days by trypsinization in 0.05% trypsin EDTA (obtained from 0.25% Trypsin EDTA Gibco #25200056 ...
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bioRxiv - Physiology 2023Quote: ... onto a Dionex IonPac AS11-HC column (2 mm × 250 mm, 4 μm particle size, Thermo Fisher Scientific) equipped with a Dionex IonPac AG11-HC guard column (2 mm × 50 mm ...
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bioRxiv - Cell Biology 2024Quote: ... 5% v/v 2-mercaptoethanol) and fractionated by 4–12% Bis-Tris gel (Thermo Fisher Scientific Cat#NP0335BOX) using MES running buffer (Thermo Fisher Scientific Cat#NP000202) ...
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bioRxiv - Molecular Biology 2023Quote: ... cDNA was diluted 1:4 and 2 μL was used in a 20 μL qPCR Taqman assay (Invitrogen). Assay probes were used to detect cDNA from Nkx2.9 (Mm00435145_m1) ...
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bioRxiv - Physiology 2023Quote: ... equipped with a Dionex IonPac AG11-HC guard column (2 mm × 50 mm, 4 μm, Thermo Fisher Scientific). The column temperature was held at 30 °C ...
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bioRxiv - Immunology 2023Quote: ... we injected the mice intraperitoneally with 4 mg/ml of 5-ethynyl-2′-deoxyuridine (EdU) (Invitrogen, Waltham, MA) with the goal of labeling circulating monocytes to assess macrophage retention among groups ...
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bioRxiv - Immunology 2023Quote: ... or 2 µg H4K20me1 (Invitrogen) (were incubated overnight at 4 with rotation. The following day, Pierce beads (Invitrogen) were washed 3x in ChIP buffer ...
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bioRxiv - Microbiology 2023Quote: ... supplemented with 25 mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) and 10% Hi-FBS (Thermo Fisher Scientific). BSC-1 cells (ATTC ...
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bioRxiv - Developmental Biology 2023Quote: ... cells were cultured from day 2 to day 4 in advanced RPMI 1640 (A-RPMI) (Gibco™ 12633012) supplemented with GlutaMax and 7.5 μM CHIR99021 ...
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Mitochondrial Apolipoprotein MIC26 is a metabolic rheostat regulating central cellular fuel pathwaysbioRxiv - Cell Biology 2023Quote: ... equipped with a Dionex IonPac AG11-HC guard column (2 mm X 50 mm, 4 µm, Thermo Scientific) at 30°C ...
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bioRxiv - Neuroscience 2024Quote: ... onto a Dionex IonPac AS11-HC column (2 mm × 250 mm, 4 μm particle size, Thermo Fisher Scientific) equipped with a Dionex IonPac AG11-HC guard column (2 mm × 50 mm ...
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bioRxiv - Neuroscience 2024Quote: ... equipped with a Dionex IonPac AG11-HC guard column (2 mm × 50 mm, 4 μm, Thermo Fisher Scientific). The column temperature was held at 30°C ...
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bioRxiv - Neuroscience 2024Quote: ... Neurons were washed 4 times with 2× SSC and stained with 0.5 µg/ml DAPI (Thermo Scientific, 62248) in 2× SSC for 1 min ...
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bioRxiv - Microbiology 2021Quote: ... optic density at 600 nm (OD600) was determined every 2 h during 36 h using a NanoDrop™ ONE spectrophotometer (ThermoFisher Scientific, USA).
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bioRxiv - Pharmacology and Toxicology 2021Quote: Mouse primary PTECs were plated onto a chambered cover glass and incubated in phenol-red-free low glucose (5.5mM) medium for one day and stained with 200nM MitoSOXTM Red and 2 μg/ml Hoechst 33343 (Thermo Fisher Scientific, Waltham, MA) for 30 min at 37°C ...
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bioRxiv - Physiology 2023Quote: ... incubated at room temperature for one hour with Alexa Fluor 568-conjugated goat anti-mouse IgG (2 μg/ml, Molecular Probes, Cat #A11004) and Alexa Fluor 647-conjugated donkey anti-rabbit IgG (2 μg/ml ...
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bioRxiv - Physiology 2023Quote: ... incubated at room temperature for one hour with Alexa Fluor 647-conjugated donkey anti-mouse IgG diluted in blocking buffer (2 μg/ml, Molecular Probes, Cat #A31571) followed by washes in PBS ...
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bioRxiv - Immunology 2019Quote: ... and IL-2 concentration was measured using a mouse IL-2 ELISA kit (Invitrogen). For CFSE dilution assays ...
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bioRxiv - Neuroscience 2020Quote: ... and pMD2G—at a 2:2:1 ratio using Lipofectamine 2000 (Thermo-Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: ... Candidate drugs were diluted 2-fold seriously by medium supplemented with 2% FBS (GIBCO), penicillin (100 IU/mL) ...
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bioRxiv - Microbiology 2021Quote: ... (2) cells were incubated with 2 μg/mL BODIPY FL Vancomycin (Thermo Scientific, V34850); (3 ...
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bioRxiv - Immunology 2020Quote: ... Washing medium consisted of 2% FCS and 2% P/S in RPMI 1640 (Gibco). FACS buffer contained 2% FCS and 2mM EDTA in PBS ...
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bioRxiv - Cell Biology 2020Quote: ... 2 μg/ml goat anti-rabbit F(ab’)2 fragment (Life Technologies A-21246) and goat anti-mouse F(ab’)2 fragment secondary antibodies were used ...
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bioRxiv - Developmental Biology 2022Quote: Osteoblasts on glass coverslips were loaded with 2 µM Fura-2 AM-ester (Invitrogen) for 30 minutes at 25 °C in 107 mM NaCl ...
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bioRxiv - Neuroscience 2021Quote: ... samples were washed three times with 2× saline sodium citrate (2×SSC, ThermoFisher AM9765), and equilibrated with encoding-probe wash buffer (30% formamide (ThermoFisher AM9342 ...
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bioRxiv - Cell Biology 2019Quote: ... pH 7.4) supplemented with 2 μM fura-2-AM (F1201, Molecular Probes, Eugene, OR) and 0.01 % pluronic acid ...
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bioRxiv - Immunology 2020Quote: ... 2 mM L-Glutamine and 50 µm 2-Mercaptoethanol) with 10% FCS (all Gibco). Colon and caecal tissue were digested in an enzyme cocktail of 0.45 mg/ml collagenase V (Sigma-Aldrich) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: Caco-2 cells were seeded into LabTekII 2-well chambered coverglass (#155380, ThermoFisher Scientific) pre-coated with 0.01% Poly-L-lysine (#P4707 ...
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bioRxiv - Microbiology 2020Quote: ... with 2 Units of S1 enzyme (ThermoFisher, 2 uL of a 1:100 dilution) and 1X S1 Buffer ...
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bioRxiv - Microbiology 2021Quote: ... 2% fetal bovine serum (S182H-500, Eurobio) and N-2 Supplement (17502048, Thermo Fisher) at 37°C and 5% CO2.
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bioRxiv - Microbiology 2022Quote: ... (2) cells were incubated with 2 μg/mL BODIPY FL Vancomycin (Thermo Scientific, V34850) for 30 min ...
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bioRxiv - Neuroscience 2023Quote: ... and 5ng/mL bovine fibroblast growth factor-2 (bFGF-2, #13256-029, Gibco™)) (proliferative conditions) ...
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bioRxiv - Cancer Biology 2023Quote: ... animals were subjected to intracardiac perfusion with 2% PFA-2% glutaraldehyde (A17876, ThermoFisher Scientific) in 0.1 M phosphate buffer (PB ...
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bioRxiv - Genomics 2023Quote: ... and 10 mM HEPES (N-2-hydroxyethylpiperazine-N-2-ethane sulfonic acid, Gibco 15630080), and incubated at 37°C ...
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bioRxiv - Cancer Biology 2024Quote: ... 2% SDS) and 2 µL 20 mg/mL proteinase K (25530049, Thermo Fisher Scientific). Samples were incubated overnight at 65 °C and 800 rpm ...
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bioRxiv - Cancer Biology 2024Quote: ... 2 µl 10% SDS plus 2 µl proteinase K (Thermo Fisher Scientific, Cat# EO0492) were added and incubated for 60 min 37°C ...
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bioRxiv - Cell Biology 2024Quote: Fura-2 acetoxymethyl ester (fura-2/AM) was from Molecular Probes (Leiden, The Netherlands). High-glucose Dulbecco’s modified Eagle’s medium (DMEM) ...
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bioRxiv - Bioengineering 2024Quote: ... and 5 ng/ml of fibroblast growth factor-2 (FGF-2) (all from Gibco). Cells were seeded at a density of 3200 cells/cm2 until they reached 90% confluency ...
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bioRxiv - Cancer Biology 2021Quote: Relative cell proliferation rates were assayed using an MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay kit (Vybrant™ MTT Cell Proliferation Assay Kit, Invitrogen™ ...
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bioRxiv - Cancer Biology 2022Quote: ... 10 μL of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT, concentration 5 mg/mL, Invitrogen, cat. M6494) in PBS was added to each well containing culture medium and incubated for 2.5 h at 37 °C ...
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bioRxiv - Biochemistry 2019Quote: ... Peptides were desalted (Acclaim PepMap 100, 75µm × 2 cm, nano viper, C18, 3 µm, 100 Å, ThermoFisher, Waltham, MA) and separated (Acclaim ...
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bioRxiv - Cell Biology 2019Quote: ... cells were loaded with 2 µM CellEvent™ Caspase-3/7 Green Detection Reagent (Life Technologies, Carlsbad, CA, USA) according to the manufacturer’s protocol and visualized using FITC 488 nm filter ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... plates were treated with 2 μM of the CellEvent™Caspase-3/7 green detection reagent (Life Technologies, UK) for 60 minutes at 37 °C in the dark ...
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bioRxiv - Bioengineering 2021Quote: ... Medium was changed every 2-3 days and cell passages were carried out using a TrypLE Express solution (Gibco). Human TERT-immortalized gingival fibroblasts (hTERT-HGF ...