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Citations for Thermo Fisher :
2001 - 2050 of 10000+ citations for 6 Propyl 2 thioxo 2 3 dihydropyrimidin 4 1H one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: We seeded 2×105 cells / well into 6 well dishes and replaced media with 5% KSR (Knockout Serum Replacement) (Gibco) containing media the next day ...
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bioRxiv - Genetics 2021Quote: Live myofibers in the 6-well plate were stained with 2 µL of 5mg/mL of Hoechst (Molecular Probes, H1399) in 2 mL of 1X PBS for 5 minutes in a 37 °C with 5% CO2 incubator ...
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bioRxiv - Neuroscience 2019Quote: ... proliferating NSCs were plated on matrigel-coated 6- or 12-well plates and cultured in Neurobasal medium supplemented with B27 without vitamin A (2%, ThermoFisher), PenStrep (1% ...
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bioRxiv - Bioengineering 2020Quote: ... Primary fibroblast passages 2–6 were achieved in culture in 37°C in a 5% CO2 incubator with DMEM High Glucose (Gibco) with 10% FBS to 80% confluence in 100 mm Petri dishes ...
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bioRxiv - Neuroscience 2021Quote: ... samples were washed 5-6 times over 2 hours in PBT at room temperature and incubated with secondary antibodies (Invitrogen) diluted in NGS-PBT for 24 hours at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... on 12 or 15 mm coverslips in a 6-well plate and 10 nM siRNA-1 or -2 was transfected using RNAi MAX reagents (Invitrogen) according the manufacturer protocol ...
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bioRxiv - Cancer Biology 2019Quote: ... for IL-6) for 2 hours at RT and followed by incubating in 1:1000 DAPI (62248, Thermo Fisher Scientific) in PBS for 1min ...
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bioRxiv - Microbiology 2021Quote: ... for 6 min and seeding cells at a density of 2×106 cells/T75 flask (Nunc; Thermo Fisher Scientific, Singapore).
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bioRxiv - Microbiology 2021Quote: ... for 6 min and seeding cells at a density of 2×106 cells/T75 flask (Nunc; Thermo Fisher Scientific, Singapore).
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bioRxiv - Cell Biology 2021Quote: ... 2 µg DNA was mixed with 6 µL of Fugene HD in 100 µL of Opti-MEM (Thermo Fisher Scientific) and incubated at room temperature for 30 min ...
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bioRxiv - Biophysics 2022Quote: ... Purified Syb2 (29–96, S61C) was labeled with 6× molar excess BODIPY FL N-(2-aminoethyl)-maleimide (BDPY) (Molecular Probes) and SN25 FL (1–206 ...
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bioRxiv - Molecular Biology 2022Quote: ... Staining of nuclei with 4ʹ,6-diamidino-2-phenylindole (DAPI) was performed with mounting solution (ProLong Diamond Antifade Mountant with DAPI (ThermoFisher)) or 50% glycerol in 1×PBS.
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bioRxiv - Biochemistry 2021Quote: ... the cells were seeded on a 6-well plate with 2 ml of DMEM medium supplemented with 5% horse serum (Gibco). At 24 hours after transfection ...
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bioRxiv - Molecular Biology 2019Quote: ... Total RNA from HEK 293T cells (N = 2 biological replicates) or whole mouse brain (N = 6 biological replicates) was extracted using TRIzol (ThermoFisher) and treated with RNase-free DNase I (Promega) ...
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bioRxiv - Cancer Biology 2019Quote: The human myeloma cell line KJON (19) was cultured in RPMI with 5 % heat inactivated human serum (HS) and 2 ng/mL interleukin (IL)-6 (Gibco, Thermo Fisher Scientific ...
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bioRxiv - Immunology 2019Quote: ... Transient transfections of wild-type or mutated MCM10-expressing plasmids were performed by transfecting 2×106 cells with 6 μg of DNA using Lipofectamine 2000 according to manufacturers’ instructions (Invitrogen).
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bioRxiv - Genomics 2020Quote: ... cells were harvested and grown for 5-6 passages in NPC expansion medium (selection medium with the addition of 2% B27 [GIBCO] ...
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bioRxiv - Systems Biology 2019Quote: ... for 30 minutes at 37C and plated with 2 million C57Bl/6 mouse embryonic fibroblasts as feeder cells (Gibco A34960) and with serum+LIF media composing of ...
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bioRxiv - Biophysics 2022Quote: ... Fractions containing S15 were consolidated and concentrated to ∼2 mg/mL using a 6 mL 3K molecular weight cutoff centrifugal concentrator (ThermoFisher), before being aliquoted and flash-frozen in liquid nitrogen for long-term storage at -80°C.
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bioRxiv - Neuroscience 2022Quote: ... CHO cells were plated at 1 x 106 cells/well in a 6-well plate and cultured for 24 hr in 2 ml of F12 medium (Gibco) supplemented with 10% FBS and 1% Glutamax (Gibco ...
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bioRxiv - Biophysics 2023Quote: ... Adherent semiconfluent HEK293SG cells cultured in DMEM:F12 medium supplemented with 10% FBS were transfected in 6-well plates with 2 μg/well of plasmid DNA complexed with Lipofectamine 2000 reagent (Invitrogen). The cells were incubated for 48 hrs at 37 °C under 7% CO2 ...
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bioRxiv - Plant Biology 2023Quote: ... Protein pellets were resuspended in urea buffer (6 M urea, 2 M thiourea, 100 mM ammonium bicarbonate, pH 8.0) and quantified using EZQ (Invitrogen/Life Technologies).
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bioRxiv - Cell Biology 2023Quote: The ROS dye assay was performed using Di(Acetoxymethyl Ester) (6-Carboxy-2’,7’-Dichlorodihydrofluorescein Diacetate) ROS dye (Invitrogen, D23844), a fluorogenic dye that is converted to 6-CarboxyFluorescein ...
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bioRxiv - Cell Biology 2023Quote: ... HeLa cells at ∼50% confluency in 6-well plates were incubated with 2 mL Opti-MEM-I medium (Gibco, 31985062) supplied with 6 µL Lipofectamine 2000 transfection reagent (Invitrogen ...
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bioRxiv - Immunology 2023Quote: ... Cells were transfected in 6-well plates with 2 µg of the indicated constructs in the presence of Lipofectamine 2000 (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... 2 µg DNA was mixed with 6 µL of FuGENE HD in 100 µL of Opti-MEM (Thermo Fisher Scientific) and incubated at RT for 10 min before being added to HeLa cells grown in 35-mm glass-bottomed dishes (AGC Technology Solutions ...
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bioRxiv - Biochemistry 2023Quote: Doxycycline-inducible C2C12 cells were seeded for most treatments at 0.1X106 cells per well in 6-well plates with 2 ml of DMEM [high glucose DMEM with pyruvate (Gibco, 12800), 3.7 g/L NaHCO3 ...
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bioRxiv - Bioengineering 2019Quote: DSS-treated mice received 3 cycles of 2% (weight/volume) DSS (40,000 MW, Alfa Aesar, Thermo Fisher, France) in drinking water for 5 days ...
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bioRxiv - Cell Biology 2019Quote: ... and induced to differentiate in myotubes for 2-3 days in differentiation media (DM: IMDM (Gibco, 21980-032) + 2% of horse serum (Gibco ...
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bioRxiv - Neuroscience 2021Quote: ... Cultured neurons were incubated with 3 μg/mL of the ratiometric calcium indicator Fura-2 AM (Life Technologies) in external recording solution for 30 min at 37°C and 5% CO2 ...
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bioRxiv - Neuroscience 2020Quote: ... was aspirated and the fixed cells treated with 2-3 drops of ProLong Gold Antifade Mountant (Invitrogen #P36930) before imaging ...
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bioRxiv - Plant Biology 2020Quote: ... seedlings were washed for 2-3 min in water and transferred to a chambered cover glass (Thermo Scientific), and imaged either as described above or using a Leica DM5500 wide field microscope (GFP filtercube ex ...
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bioRxiv - Molecular Biology 2020Quote: ... RNA was reverse-transcribed by addition of 3 μl of RT mix (2 μl 5x SSIV buffer [Invitrogen] ...
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bioRxiv - Microbiology 2020Quote: ... Myoblat and myotubes viability was determined by 3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide (MTT; Life Technologies) metabolization.
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bioRxiv - Cancer Biology 2020Quote: ... then assayed in a standard MTT assay: 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (ThermoFisher, Waltham, MA) [39] ...
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bioRxiv - Cancer Biology 2021Quote: ... After 3 and 7 days of treatment cells were stained with 2% crystal violet (CV) (40583100, Acros Organics, Fisher Scientific ...
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bioRxiv - Biochemistry 2022Quote: ... equipped with an Acclaim Pepmap C18 trap column (2 cm * 75 µm, particle size: 3 µm; Thermo Fisher) and a C18 analytical column (50 cm * 75 µm ...
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bioRxiv - Genetics 2021Quote: ... Gels were run at 120V for 2-3 hours in Bolt MES SDS Running Buffer (Thermo Fisher Scientific) prior to protein transfer to Amersham Protran nitrocellulose blotting membrane (GE Healthcare ...
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bioRxiv - Neuroscience 2021Quote: ... supplemented with NeuroCult™ SM1 neuronal supplement (STEMCELL), L-glutamine (2 mM, PAA) and 3% horse serum (Invitrogen) at 37°C in 5% CO2 ...
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bioRxiv - Cancer Biology 2022Quote: Tail and ear fragments from C57/B6 or mT/mG mice were dissected and incubated in 2-3 hours at 37 °C in 950 µL of Dulbecco’s modified Eagle’s medium (DMEM, Invitrogen), supplemented with 10% inactivated fetal bovine serum (FBSi ...
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bioRxiv - Bioengineering 2019Quote: ... Lysis was done in 2% SDS for 3 min at 95 °C in a thermomixer (Thermo Fisher Scientific), followed by digestion of DNA with Benzonase at 37 °C for 1.5 h ...
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bioRxiv - Cell Biology 2020Quote: ... and induced to differentiate in myotubes for 2-3 days in differentiation media (DM: IMDM (Gibco, 21980-032) + 2% of horse serum (Gibco ...
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bioRxiv - Cell Biology 2021Quote: ... cells were treated with DMNB-caged cAMP (4,5-Dimethoxy-2-Nitrobenzyl Adenosine 3’,5’-Cyclicmonophosphate, Molecular Probes, D1037) for at least 30 minutes prior to imaging at a final concentration of 1 mM ...
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bioRxiv - Immunology 2020Quote: Cell viability was estimated by a quantitative colorimetric assay described for human granulocytes which were based on metabolic reduction of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT, Invitrogen) into coloured product formazan (Oez ...
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bioRxiv - Developmental Biology 2022Quote: ... They were then immobilized on glass slides using 2% agarose and injected with 1,1’-dioctadecyl-3,3,3′,3′-tetramethylindocarbocyanine perchlorate (DiI, Molecular Probes) or 3,3′-dioctadecyloxacarbocyanine perchlorate (DiO ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were washed twice with PBS and loaded with 3 µM Fura-2 AM (Thermo Fisher, Waltham, MA) diluted in a modified Krebs-Ringer buffer solution [KRBH ...
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bioRxiv - Plant Biology 2022Quote: ... equipped with an Acclaim Pepmap C18 trap column (2 cm · 75 µm, particle size: 3 µm; Thermo Fisher) and a C18 analytical column (50 cm · 75 µm ...
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bioRxiv - Immunology 2022Quote: ... Primary B cells were plated at 2-3×106 cells/ml in primary B cell medium (DMEM (Gibco) containing 10% FBS (Sigma) ...
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bioRxiv - Microbiology 2023Quote: ... the cells were loaded with 2 µM CellEvent™ Caspase-3/7 Green Detection Reagent (Thermo Fisher Scientific), according to the manufacturer’s instructions for kinetic assays and fluorescence in the live cells ...
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bioRxiv - Pathology 2023Quote: Tail and ear fragments from mT/mG mice were dissected and incubated in 2-3 hours at 37 °C in 950 µL of Dulbecco’s modified Eagle’s medium (DMEM, Invitrogen), supplemented with 10% inactivated fetal bovine serum (FBSi ...