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Citations for Thermo Fisher :
2051 - 2100 of 10000+ citations for 6 Propyl 2 thioxo 2 3 dihydropyrimidin 4 1H one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2023Quote: ... 3-5 million cells or approximately 15 ug of DNA crosslinked with 2% PFA (Fisher Scientific F79-500) were used as input per reaction ...
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bioRxiv - Cell Biology 2023Quote: ... cells were treated with DMNB-caged cAMP (4,5-Dimethoxy-2-Nitrobenzyl Adenosine 3’,5’-Cyclicmonophosphate, Molecular Probes, D1037) for at least 30 min prior to imaging at a final concentration of 1 mM ...
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bioRxiv - Cancer Biology 2023Quote: Cell proliferation was assayed by reduction of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT; Invitrogen, M6494). MTT was freshly dissolved into PBS at a stock concentration of 12 mM and diluted into phenol red-free DMEM with 10% FBS for a final MTT concentration of 2 mM ...
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bioRxiv - Biochemistry 2022Quote: ... 2-3 µL of 5 µM protein solution was introduced directly into Q-Exactive UHMR mass spectrometer (ThermoFisher) through gold coated capillary needles that were prepared in-house30 ...
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bioRxiv - Microbiology 2023Quote: ... Proteins were migrated for 2-3 h at 120 V in NuPAGE MOPS SDS running buffer (#NP0001, Invitrogen) and transferred onto a PVDF membrane (#1704156 ...
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bioRxiv - Molecular Biology 2023Quote: ... Mix 1 contains 3 siRNAs (CliniSciences, CRH7929) and Mix 2 contains two siRNAs (ThermoFisher Scientific, 1299001 and 4392420). As a negative control ...
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bioRxiv - Plant Biology 2023Quote: ... 3 µg RNA from each sample was used to mix with 2×RNA loading dye (Thermo Fisher Scientific), denatured at 65℃ for 10 min ...
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bioRxiv - Genomics 2024Quote: ... Fresh medium was added every 2-3 days and cells were passaged using 1X TrypLE Express (Life Technologies).
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bioRxiv - Cancer Biology 2024Quote: ... or using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) reagent (Fisher Scientific, AC158992500, Waltham, MA, USA) at 570 nm ...
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bioRxiv - Microbiology 2024Quote: ... The filtrate was acidified to a pH of 2-3 with OmniTrace HCl (ThermoFisher Scientific, Waltham, MA, USA) and extracted via solid phase extraction with styrene-divinylbenzene polymer columns (Bond Elut PPL ...
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bioRxiv - Immunology 2020Quote: ... before staining with 3 μM fluo-4 (Invitrogen) and 4 μM Fura Red (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2019Quote: ... Total serum F(ab’)2 was then applied to a one ml custom made affinity column comprised of IFX-F(ab’)2 coupled to NHS-activated agarose beads (Thermo Fisher Scientific). The purified serum F(ab’)2 were recycled three times over the affinity column ...
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bioRxiv - Microbiology 2019Quote: ... 1 mM DTT supplemented with Pierce Protease and Phosphatase Inhibitor Mini Tablets (40 μL/mL of stock solution – one tablet / 2 mL H2O, Thermo Scientific, 88668) and 25 U/mL of benzonase (Merck-Millipore ...
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bioRxiv - Microbiology 2019Quote: ... supplemented with Pierce Protease and Phosphatase Inhibitor Mini Tablets (40 μL/mL of stock solution – one tablet / 2 mL H2O, Thermo Scientific, 88668). Samples were kept on ice for 30 minutes and centrifuged for 15 minutes ...
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bioRxiv - Immunology 2022Quote: ... with one notable difference being that sodium azide was excluded from FACS buffer (2% FBS and 1mM EDTA [Fisher Scientific] in PBS) to ensure optimal CD138 staining (Wilmore et al. ...
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bioRxiv - Cancer Biology 2019Quote: ... cells were transfected with 4.5 μg of either pSpCas9(BB)-2A-GFP control plasmid DNA (Ran et al, 2013) or one of 2 different TBK1 targeting plasmids using Lipofectamine® LTX with Plus™ Reagent (Invitrogen). Briefly ...
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bioRxiv - Microbiology 2021Quote: ... 2 µL of one-tenth diluted RT products were amplified in 2X ABsolute qPCR SYBR Green Mix (Thermo Fisher Scientific, Illkirch, France) with 1.6 µM of specific primers with an QuantStudio™ 6 Flex system (Applied ...
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bioRxiv - Immunology 2022Quote: Preparation of CD5L-coated pHrodo particles: one vial containing 2 mg of particles (pHrodo™ Red E. coli BioParticles™ Conjugate for Phagocytosis, ThermoFisher) were reconstituted with 500 μL of TBS with 5 mM CaCL2 and sonicated for 5 min ...
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bioRxiv - Immunology 2023Quote: ... 2,2′-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid solution (Invitrogen, 002024) was added to the wells as the coloring substate for HRP ...
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bioRxiv - Neuroscience 2021Quote: ... in Tris-buffered PBS/ 0.1 % Tween 20 for 1 h at room temperature they were incubated overnight at 4 °C with the following antibodies: rabbit polyclonal anti-2′,3′-cyclic nucleotide 3′-phosphodiesterase (CNPase; 49 kDa; 1:1000; Thermo Fisher Scientific, Waltham, MA, USA), mouse monoclonal anti-myelin-associated glycoprotein (MAG ...
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bioRxiv - Biophysics 2022Quote: ... and 2-3 μL of the freshly phase-separated sample was placed into a chamber made on a glass slide (Fisher Scientific 3” × 1” × 1 mm). The chamber made by using double-sided tape was then sealed with a square coverslip to avoid evaporation of the sample ...
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bioRxiv - Cell Biology 2020Quote: ... Cells in one well of 6 well plates were collected in ice-cold PBS (Gibco) and lysed in 200 µL lysis buffer (50 mM Tris-HCl pH 7.0 ...
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bioRxiv - Cancer Biology 2020Quote: ... One ng/mL recombinant human interleukin (IL)-6 (Gibco, Thermo Fisher Scientific, Waltham, MA, USA), was added to the media of the IL-6 dependent myeloma cell lines INA-6 ...
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bioRxiv - Microbiology 2023Quote: Vero E6 were seeded in 6-well plates (Falcon) with one glass coverslip (Fisher Scientific) per well ...
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bioRxiv - Microbiology 2020Quote: ... containing 2% heat-inactivated autologous plasma and 2 mM L-glutamine (Life Technologies), unless otherwise stated ...
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bioRxiv - Biophysics 2020Quote: ... and stimulated with 50 U/mL recombinant interleukin-2 (IL-2, Thermo Fisher) and 5 μg/mL phytohaemagglutinin (PHA ...
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bioRxiv - Neuroscience 2022Quote: ... GlutaMAX and FGF-2 (2 ng/ml) (all reagents from Thermo Fisher Scientific) in 24-well plates for 2 weeks on coverslips coated with poly-D-lysine (100 µg/ml) ...
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bioRxiv - Microbiology 2021Quote: ... 2 min) to nitrocellulose membranes using an iBlot 2 Dry Blotting System (Invitrogen). Membranes were briefly washed in PBS-T (0.1% Tween-20/PBS) ...
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bioRxiv - Synthetic Biology 2020Quote: ... at 2 mA cm−2 in a semi-dry electroblotter (Thermo Fisher Scientific) for 45 min using 1 × TBE buffer ...
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bioRxiv - Microbiology 2021Quote: ... Gibco Hepes (N-2-hydroxyethylpiperazine-N-2-ethane sulfonic acid, 0.05 mM; Invitrogen), ROCK Inhibitor Y-27632 (1 mM ...
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bioRxiv - Cell Biology 2020Quote: ... and 30 U/ml of interleukin-2 (IL-2; Thermo Fisher Scientific, PHC0026) at 37°C ...
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bioRxiv - Immunology 2021Quote: ... EBM-2/EGM-2 medium was supplemented with puromycin (1 mg/ml, Gibco) for 3 d ...
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bioRxiv - Immunology 2021Quote: ... IL-2 ELISA was performed using IL-2 Mouse Uncoated ELISA Kit (Invitrogen) following manufacturer’s protocol.
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bioRxiv - Immunology 2022Quote: ... 2 h goat anti-rabbit IgG F(ab’)2-AF488 secondary antibody (Invitrogen), 15 min DAPI (1:1000) ...
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bioRxiv - Systems Biology 2023Quote: ... 2 µL Invitrogen TURBO DNase and 2 µL SUPERase•In RNase Inhibitor (Invitrogen). After digest ...
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bioRxiv - Microbiology 2023Quote: ... for adipose fat or 2 mg/mL collagenase type 2 (ThermoFisher Scientific, 17101015) for lung samples ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Myocytes were then loaded with Fura-2 acetoxymethyl ester (Fura-2 AM; Invitrogen) as described previously (Batiste et al. ...
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bioRxiv - Neuroscience 2023Quote: ... 5mM N-2-hydroxyethyl piperazine-N-2-ethane sulfonic acid (HEPES; Gibco, 15630080), and cOmplete ...
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bioRxiv - Immunology 2023Quote: ... 5x10-5 M 2-mercaptoethanol (2-ME) and 5 mM HEPES (all Invitrogen).
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bioRxiv - Cancer Biology 2023Quote: ... cells were incubated with 2 μM Fura-2 AM (Thermo Fisher Scientific, F1225) for 30 min at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... they were loaded with 2 µM Fura-2-AM (Invitrogen/Thermo Fisher Scientific) dissolved in normal Ca2+-buffer (150 mM NaCl ...
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bioRxiv - Neuroscience 2024Quote: ... they were loaded with 2 µM Fura-2-AM (Invitrogen/Thermo Fisher Scientific) dissolved in normal Ca2+-buffer (150 mM NaCl ...
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bioRxiv - Neuroscience 2024Quote: ... 150uL of Tyrode’s containing 2 uM Fura 2-AM (Invitrogen F1221, Lot 2559176)/ Pluronic F-127 (Invitrogen P3000MP ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... 7-Diethylamino-3-(4'-Maleimidylphenyl)-4-Methylcoumarin (CPM) was purchased from Thermo Scientific Life Technologies (Grand Island ...
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bioRxiv - Neuroscience 2019Quote: Plated DRG cultures were loaded with fura-2 by 1-hour incubation with a mixture of 2 μM fura-2 AM (Thermo Fisher Scientific) and 0.01% pluronic acid (Sigma-Aldrich ...
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bioRxiv - Microbiology 2020Quote: ... The non-encapsidated DNA was then removed by a 1h incubation at 37°C with 4 U TURBO DNase enzyme (Invitrogen), 1X TURBO DNase Buffer ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were then incubated with primary antibodies in 10% NGS/NDS in PBST over night at 4°C following 1h incubation at 24°C with appropriate Alexa488/568 coupled secondary antibodies (1:1000, Invitrogen). Cell nuclei were stained with DAPI and final images were acquired using Leica TCS SP8 confocal microscope (Leica Microsystems ...
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bioRxiv - Biophysics 2020Quote: ... fixed cells are incubated 1h at room temperature with Myosin 4 Monoclonal Antibody conjugated with Alexa Fluor 488 (MF20, Thermofisher) at 5μg/mL (1:100 dilution ...
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bioRxiv - Plant Biology 2022Quote: ... and immunocomplexes were next incubated for 1h at 4°C with 40 μL of Dynabeads Protein A (Thermo Fisher Scientific). The beads were washed 2 × 5 min in ChIP Wash Buffer 1 (0.1% SDS ...
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bioRxiv - Neuroscience 2023Quote: ... Dissected retinas were either fixed in 4% formaldehyde (MilliporeSigma, Cat.# FX0415-4) in PBS for 2 hours at room temperature or lysed with RIPA buffer (Thermo Fisher Scientific, Cat.# 89900) containing protease and phosphatase inhibitors (Thermo Fisher Scientific ...