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Citations for Thermo Fisher :
2001 - 2050 of 10000+ citations for 6 Chloro 9 fluorobenz 9 isoquinoline 5 10 dione since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... and insoluble samples and were denatured in reducing Laemmli buffer at 100°C for 5-10 min prior to loading onto a 4-20%Tris-Gly SDS-PAGE gel (Life Technologies). Proteins were then transferred to a nitrocellulose membrane using an iBlot2 (Life Technologies) ...
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bioRxiv - Pathology 2021Quote: Small volumes (5-10 µL) of blood were collected using tail-prick and heparinized microhematocrit tubes (Fisher Scientific cat. # 22-362566), placed on ice in 15 mL sample tubes ...
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bioRxiv - Cell Biology 2020Quote: ... were grown in a humidified incubator at 37°C and 5% CO2 in DMEM/F12 medium supplemented with 10% fetal bovine serum and 1% penicillin/streptomycin (GIBCO/Life technologies). Cells were plated at approximately 15000 cells/ml on patterned polyacrylamide gels and left to spread for 3 to 4 hours before imaging.
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bioRxiv - Microbiology 2020Quote: ... and HepG2 (ATCC HB-8065) were cultured at 37°C under 5% CO2 in DMEM supplemented with 10% fetal calf serum (10500064, Life Technologies), L-glutamine 20 μM (25030024 ...
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bioRxiv - Microbiology 2021Quote: ... HepG2/CD8140 and Hepa1-6 cells (ATCC CRL-1830) were cultured at 37°C under 5% CO2 in DMEM supplemented with 10% fetal calf serum and antibiotics (Life Technologies), as described7 ...
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bioRxiv - Immunology 2020Quote: ... tumor cells were plated at 1 × 104 cells per well and incubated overnight at 37°C/5% CO2 in RPMI-1640 medium supplemented with 10% heat-inactivated FBS and GlutaMAX (Life Technologies) in a 96-well flat-bottom plate ...
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bioRxiv - Genetics 2020Quote: ... The PCR analysis was carried out with 1 μl of cDNA as a template in a reaction volume of 10 μl containing 5 μl of Power SYBR Green PCR Master Mix (Thermo-Fisher Scientific) and 200 nM of each primer ...
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bioRxiv - Genetics 2020Quote: Samples of feline cerebral cortex (5 – 10 mg) were homogenized in 400 µl of RIPA buffer (PIERCE, ThermoFisher, Waltham, MA, USA) with a hand-held micro-pestle for 30 sec ...
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bioRxiv - Neuroscience 2022Quote: ... Each fraction was then loaded onto a homemade trap column (200 μm × 5 cm bed volume) packed with POROS 10R2 10 μm resin (Applied Biosystems), followed by a homemade analytical column (50 μm × 50 cm bed volume ...
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bioRxiv - Molecular Biology 2022Quote: ... were cultured in DMEM medium supplemented with 10% heat-inactivated FBS and 1% penicillin-streptomycin at 37°C in 5% CO2 (ThermoFisher Scientific). HEK293T cells were cultured in DMEM medium supplemented with 10% FCS and 1% penicillin-streptomycin at 37°C in 5% CO2.
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bioRxiv - Molecular Biology 2022Quote: ... were cultured in Medium 106 (Cascade Biologics) supplemented with 10% Low Serum Growth Supplement (LSGS) and 1% penicillin/ streptomycin at 37°C in 5% CO2 (ThermoFisher Scientific).
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bioRxiv - Biochemistry 2022Quote: HeLa cells were cultured at 37°C and 5% CO2 in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% FBS (10270, Fisher Scientific™, USA), 1x penicillin-streptomycin (P0781-100ML ...
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bioRxiv - Bioengineering 2022Quote: ... Skin samples embedded in paraffin blocks were sectioned into 5-10 μm thick sections using a microtome (Microm HM355S, Thermo Scientific). Tissue sections were collected on glass slides (Fisherbrand™ Superfrost plus ...
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bioRxiv - Cell Biology 2022Quote: ... in a reaction volume of 10 μL that contained the following reagents: 5 μL “TaqMan Fast Advanced Master Mix (4444557; Applied Biosystems), 0.5 μL TaqMan Gene expression assay 20× (ThermoFisher Scientific) ...
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bioRxiv - Cell Biology 2022Quote: YAP–HaloTag CRISPR knock-in U-2 OS cells were cultured at 37°C and 5% CO2 in DMEM supplemented with 10% fetal bovine serum (FBS; Gibco, 26140079), 100 U/ml (1% ...
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bioRxiv - Genomics 2022Quote: ... Purified RNA was eluted with 10 µl of pre-heated Elution Buffer (10 mM Tris-HCl pH 7,5) for 5 min at 80°C and quantified with a Qubit Fluorometer (Thermo Fisher Scientific) using the RNA HS Assay Kit (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2022Quote: ... were seeded at 5 X 103 cells/cm2 in a Matrigel-coated plate and cultured with DMEM medium supplemented with 10% FBS (Life Technologies) and 2 mM L-glutamine ...
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bioRxiv - Plant Biology 2022Quote: ... For RT-PCR and RT-qPCR beads were washed in 20 μl 1 x SSIV buffer before resuspension in 12 μl sterile H2O with 1 μl dNTP 10 mM each mix and incubated for 5 min at 50 °C in a Proflex PCR machine (Applied Biosystems). Then the following were added 4 μl 10 x SSIV buffer ...
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bioRxiv - Physiology 2023Quote: ... and then radiolabeled with 5 μCi/mL 3H-cholesterol and cholesterol enriched with 10 μg/mL of acetylated LDL (AcLDL, Invitrogen, MA) for 48 hr ...
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bioRxiv - Neuroscience 2022Quote: ... cultures were incubated at 37⁰C and 5% CO2 in a medium containing 10% Fetal Bovine Serum (FBS) in Neurobasal-A supplemented with GlutaMAX and gentamicin (Fisher Scientific). After 1 hour ...
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bioRxiv - Synthetic Biology 2022Quote: ... a 1:10 dilution was performed and 2 μl of diluted DNA was amplified by means of a QuantStudio 5 (Applied Biosystems) thermal cycler using the SYgreen Blue Mix (PCRbio ...
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bioRxiv - Molecular Biology 2022Quote: IP samples were eluted with 40 µl 1xLDS buffer adjusted to 10 mM DTT (5 min, 90°C) and separated on 4-12% Bis-Tris NuPAGE gels (Thermo Fisher) followed by colloidal coomassie staining (Instant Blue ...
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bioRxiv - Neuroscience 2022Quote: ... quiescence was induced over 5-10 days by replacing “complete activated media” with “complete quiescent media”: Neurobasal-A (Gibco, 10888-022) supplemented with 2% B27 minus vitamin A (Gibco ...
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bioRxiv - Biophysics 2022Quote: ... Frogs are euthanized by immersion for 10 min in 1 L water + 5 g Tricaine-S (Pentair via Fisher Scientific #NC0342409) + 10 g sodium bicarbonate (Fisher Scientific # MK-7396-500) ...
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bioRxiv - Microbiology 2023Quote: ... BHK-21 (ATCC, CRL-3216) cells were cultured in 10% FBS/DMEM supplemented with 5% tryptose phosphate broth (Thermo Fisher Scientific) at 37°C and 5% CO2.
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bioRxiv - Molecular Biology 2023Quote: ... Samples were washed three times with PME buffer for 10 minutes on ice and mounted on glass slides in PME buffer plus 5 µg/L Hoechst 33342 (ThermoFisher Scientific) and squashed with a cover slip before sealing.
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bioRxiv - Neuroscience 2023Quote: ... reactions were performed in triplicates in a final volume of 10 μL with 5 ng of RNA as template using the TaqMan Scn1a (Mm00450580_m1) gene expression assay (Applied Biosystems, Thermo Fisher Scientific ...
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bioRxiv - Immunology 2023Quote: ... 20 ng ml-1 GM-CSF (Biozym) or X38-Ag8 supernatant (1%) and from day 5 on additionally with 10 ng ml-1 IL-4 (Life technologies). At day 7 ...
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bioRxiv - Immunology 2023Quote: ... using 75 µm inner diameter nanocapillaries with PicoTip emitters (NewObjective # PF360-75-10-CE-5) packed with C18 resin (2.6 µm, 150Å, Thermo Fisher Scientific). Fractionated peptides were separated over 3- and 4-hour gradients with increasing concentrations of acetonitrile from 0% to 95% in 0.125% formic acid ...
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bioRxiv - Biochemistry 2023Quote: ... washed twice in DMEM with 10% CCS and suspended in Williams E medium supplemented with 5 % CCS and 1 mM glutamine (Invitrogen, CA). To separate live from dead cells ...
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bioRxiv - Microbiology 2023Quote: ... and 1 µL of template DNA (5-10 ng) were run on 96-well plates on a StepOne Plus Real-Time PCR system (ThermoFisher Scientific). A melting curve analysis was carried out to confirm the amplification of a single product in each reaction ...
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bioRxiv - Cancer Biology 2023Quote: ... and siRNA non-targeting control siRNA pools (Dharmacon D-001810-10-05) were mixed with Lipofectamine RNAiMax (5 µL, ThermoFisher 13778075) in a total of 200 µL Optimem (Life Technologies 31985-062 ...
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bioRxiv - Cell Biology 2023Quote: ... the nucleus was counterstained with 0.5 µg/mL 4’6-diamidino-2-phenylindole (DAPI) for 10 minutes and slides were mounted with Prolong® Gold (Life Technologies).
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bioRxiv - Molecular Biology 2023Quote: ... Serial 10-fold dilutions of gene fragments were performed in sheared salmon sperm DNA 5 µg mL-1 (m/v) (Thermofisher, LT) diluted in Tris-EDTA (TE ...
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bioRxiv - Biochemistry 2023Quote: ... DNA migration was detected by a 5 min incubation with SYBR Gold Nucleic Acid Gel Stain (Invitrogen #S11494, 1/10, 000) and revealed using a Chemidoc MP Immaging System (BIO-RAD) ...
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bioRxiv - Bioengineering 2023Quote: ... 95% humidity and 5% CO2 for 10 min before being washed three times with phosphate buffered saline (PBS, 10010023, Gibco™).
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bioRxiv - Neuroscience 2023Quote: ... Slides were immersed in distilled water for 5 minutes to remove OCT before immersion in chilled 10% neutral buffered formalin (STL286001, Fisher Scientific) for 15 minutes.
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bioRxiv - Molecular Biology 2023Quote: ... 5 μg of the corresponding plasmids supplemented with 10 μl of P3000 in 125 μl Opti-MEM (31985062, Thermo Fisher Scientific), was mixed with 7.5 μl Lipofectamine 3000 reagent in 125 μl Opti-MEM ...
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bioRxiv - Developmental Biology 2023Quote: ... coated dishes and grown at 37ºC in a HERAcell 150i or 160i 10% CO2 and 5% O2 incubator (Thermo Fisher Scientific). Cells were passaged every 4-5 days according to confluence as in (Wahlin et al ...
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bioRxiv - Evolutionary Biology 2023Quote: ... and an aliquot of 5-10% was analysed on a nanoAcquity UPLC (Waters Inc.) connected to a Q Exactive mass spectrometer (Thermo Scientific) equipped with a Digital PicoView source (New Objective) ...
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bioRxiv - Cell Biology 2023Quote: ... ESCs (5 × 10^4 cells/well) were dissociated into single cells with 0.5mM EDTA (15575020, Gibco; Thermo Fisher Scientific, MA, USA) after exposure to ROCK inhibitor ...
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bioRxiv - Cell Biology 2023Quote: ... ESCs (5 × 10^4 cells/well) were dissociated into single cells with 0.5mM EDTA (15575020, Gibco; Thermo Fisher Scientific, MA, USA) after exposure to ROCK inhibitor ...
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bioRxiv - Plant Biology 2023Quote: Real-time PCR was performed using a 10 μl reaction mixture containing 5 μl 2x TaqMan Universal PCR Master Mix (Applied Biosystems), 500 nM of each primer ...
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Machine Learning Ensemble Directed Engineering of Genetically Encoded Fluorescent Calcium IndicatorsbioRxiv - Bioengineering 2023Quote: ... Cells were washed 2x by spinning at 0.7 rcf for 5 minutes at room temperature and removing supernatant + resuspending in 10 mLs of Neuronal Basal Media (Invitrogen; 10888022) supplemented with B27 (Invitrogen ...
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bioRxiv - Microbiology 2023Quote: ... and ATTO647N (Atto-Tec) were dissolved in DMSO (10 and 5 mg.mL-1, respectively) while octadecyl rhodamine B chloride (R18, Thermo Fisher Scientific) was dissolved in ethanol (10 mM).
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bioRxiv - Bioengineering 2022Quote: All vectors were denatured at 100°C for 5 min using Laemmli sample buffer and separated by SDS-PAGE on 10% Tris-glycine polyacrylamide gels (Life Technologies). Precision Plus Protein All Blue Standards (BioRad ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were released from the beads by heating at 85℃ for 10 minutes in 20 µL 5 mM DTT and 1x LDS (ThermoFisher Scientific) and 1 mM free biotin (ThermoFisher Scientific) ...
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bioRxiv - Neuroscience 2023Quote: ... Fractions were tested for protein concentration using 5 μL of protein sample mixed with 10 μL Coomassie Protein Assay reagent (ThermoFisher Scientific). Pooled fractions were concentrated to 4 mL using 10 kDa molecular weight cut-off spin columns (Millipore ...
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bioRxiv - Cell Biology 2023Quote: ... Cells obtained from fractions 2–5 were combined according to the genotype and expanded in α-MEM containing 10% FBS (Gibco), 100 U/ml penicillin ...
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bioRxiv - Microbiology 2023Quote: ... cDNA was synthesized from 50 to 200 ηg (5–10 μl) of RNA using the Verso cDNA synthesis kit (Thermo scientific).