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Citations for Thermo Fisher :
2101 - 2150 of 10000+ citations for TIM 3 Human HEK 293 Fc His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2019Quote: HEK-293T and MDBK cells were maintained in Dulbecco's modified Eagle's medium (DMEM; Gibco-Invitrogen) containing 10% fetal bovine serum ...
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bioRxiv - Immunology 2021Quote: ... The HEK-293T cells were cultured with Dulbecco’s Modified Eagle Medium (DMEM, Gibco, 11965-092) and 10% fetal bovine serum (Gibco ...
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bioRxiv - Immunology 2021Quote: ... HEK-293T cells were transfected with the FRG construct using Lipofectamine™ 3000 (Invitrogen, # L3000015). Supernatant was collected for 7 days and the antibody was purified using a protein A column (ThermoFisher scientific ...
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bioRxiv - Neuroscience 2020Quote: ... RRID:CVCL_0063) and were maintained in standard HEK Media: DMEM (DMEM High glucose, pyruvate; Gibco 11995081) + 10% FBS (Qualified US Origin ...
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bioRxiv - Genomics 2021Quote: ... Detachment of HEK cells for passaging was performed using the TrypLE reagent (Thermo Fisher, #12605010). All cell lines were cultured at 37°C ...
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bioRxiv - Genomics 2022Quote: ... HEK- 293T cells were maintained in high glucose DMEM medium 1X with L-Glutamine (Invitrogen), supplemented with 100 U/mL penicillin and 100 mg/mL streptomycin (Gibco ...
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bioRxiv - Microbiology 2022Quote: ... Transfection of HEK-293T cells was carried out using Lipofectamine 2000 (Thermo Fisher Scientific - Invitrogen). Transfection conditions used a ratio of 1 µg total DNA (including all three plasmids ...
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bioRxiv - Microbiology 2022Quote: ... Transfection of HEK-293T cells was carried out using Lipofectamine 2000 (Thermo Fisher Scientific - Invitrogen). Transfection conditions used a ratio of 1 µg total DNA (including all three plasmids ...
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bioRxiv - Biophysics 2022Quote: HEK 293T/17 cells (ATCC) were maintained in high glucose Dulbecco’s Minimum Essential Media (Gibco) supplemented with 10% fetal bovine serum (Atlanta Biologicals) ...
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bioRxiv - Neuroscience 2024Quote: HEK-293T cells were transfected after reaching 70% confluency via Lipofectamine 3000 (Thermo Fisher Scientific) (2.5 μg total cDNA ...
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bioRxiv - Molecular Biology 2024Quote: ... 20,000 HEK-293T cells were seeded in each well of a LabTek chamberslide (#055071, Nunc). Cells were co-transfected using FuGENE 6 with 100 ng of pCMV6-NR2F2-WT or p.Arg246His and 100 ng of pCMX-NR5A1 ...
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bioRxiv - Neuroscience 2024Quote: HEK-293T and HeLa cells were maintained in Dulbecco’s modified Eagle medium (DMEM) (Gibco, 1056910) supplemented with 10% heat inactivated Fetal Bovine Serum (FBS ...
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bioRxiv - Microbiology 2024Quote: Expression of the full-length LUJV GPC was carried out in HEK 293F cells (Invitrogen) using FreeStyle Medium (Life Technologies) ...
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bioRxiv - Immunology 2024Quote: ... and HEK-293T cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM; Life Technologies, USA), while AsPC-1 ...
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bioRxiv - Microbiology 2023Quote: HEK 293T and Cos7 cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM) (ThermoFisher 11965118) supplemented with 10% heat inactivated fetal bovine serum (FBS ...
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bioRxiv - Cancer Biology 2023Quote: ... pLP2 and pLP/VSV-G into HEK 293T cells using TurboFect (#R0531, Thermo Fisher Scientific). Lenti-virions were collected after 3 days and concentrated by ultracentrifugation at 4 °C ...
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bioRxiv - Bioengineering 2022Quote: Lenti-X HEK 293T cells were transfected using Lipofectamine™ 3000 transfection reagent (ThermoFisher Scientific) following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... Culturing media for HEK-Orai1YFP cells was additionally supplemented with 4 mg/ml G418 (ThermoFisher). For biotinylation experiments ...
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bioRxiv - Molecular Biology 2024Quote: ... UHRR or HEK-293T RNAs (5 μg) were DNased with 2 U TURBO DNase (Invitrogen) and purified using an RNA Clean & Concentrator kit (Zymo Research ...
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bioRxiv - Cancer Biology 2024Quote: ... lentivirus containing shRNA was transfected into HEK 293T cells using Lipofectamine 3000 (Invitrogen cat. L3000015) reagents per manufacturer’s manual ...
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bioRxiv - Molecular Biology 2024Quote: HEK 293T and NIH3T3 cells were cultured in Dulbecco’s modified Eagle’s medium (ThermoFisher Scientific, 61965) supplemented with 10% fetal calf serum (FCS ...
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bioRxiv - Microbiology 2020Quote: ... The plasmids were transiently transfected into Expi293F cells using ExpiFectamine™ 293 Reagent (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2020Quote: 293 cells or HeLa cells were cultured in Dulbecco’s-modified Eagle’s medium (Invitrogen, cat. no. C11995500BT) added with 10% fetal bovine serum (FBS ...
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bioRxiv - Immunology 2022Quote: FreeStyle 293-F (cat# R79007) and Expi293F cells (cat# A14528; RRID: CVCL_D615) were purchased from ThermoFisher Scientific Inc ...
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bioRxiv - Molecular Biology 2021Quote: ... and recombinant expression was undertaken using the FreeStyle™ MAX 293 Expression System (Thermo Fisher Scientific) according to the manufacturer’s guidelines ...
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bioRxiv - Immunology 2019Quote: ... 293F cells (1 × 106 cells per ml) grown in FreeStyle 293 Media supplemented with GlutaMax (GIBCO) were transfected with the SLC19A1 expression construct (1µg plasmid DNA per ml of cells ...
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bioRxiv - Bioengineering 2019Quote: ... Cells were washed with a FACS buffer containing FreeStyle™ 293 Expression Medium (Thermo Fisher Scientific) with 2% BSA and incubated with 15 µg/mL primary antibody ...
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bioRxiv - Immunology 2021Quote: ... and were expressed in Expi 293F mammalian cells using Expifectamine 293 transfection reagent (Thermo Fisher Scientific) cultured for 4-5 days ...
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bioRxiv - Cell Biology 2021Quote: ... Stable transfected HEK293T cells were generated with the Flp-In T-REx 293 cell system (Invitrogen) following the manual ...
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bioRxiv - Microbiology 2020Quote: ... VLP-WT and VLP-cvD were expressed using ExpiFectamine™ 293 Transfection Kit (Thermo Fisher Scientific) following manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: Expi293F cells were transfected with the construct of interest using the ExpiFectamine 293 Transfection Kit (Gibco) following the manufacturer’s protocol for a 50 mL culture ...
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bioRxiv - Microbiology 2022Quote: ... then re-suspended in an appropriate volume of FreeStyle 293 Expression Medium (no. 12338018; Thermo Fisher) to obtain a concentration of 1e8 cells/ml ...
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bioRxiv - Microbiology 2021Quote: ... The plasmids were transiently transfected into Expi293F cells using ExpiFectamine™ 293 Reagent (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... HEK293 cells (Flp-In 293 T-REx cell lines) were obtained from Life Technologies (R780-07) (Invitrogen ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... generated from parental Flp-In™ T-REx™ 293 cells (Thermo Fisher Scientific, MA, USA) by Flp-mediated genomic integration of an insert encoding human GLP-1R with an N-terminal SNAPf-tag and C-terminal SmBiT tag (custom lingen ...
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bioRxiv - Microbiology 2020Quote: ... The plasmids were transiently transfected into Expi293F cells using ExpiFectamine™ 293 Reagent (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... The plasmid was transiently transfected into Expi293F cells using ExpiFectamine™ 293 Reagent (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... trypsinized the cells were transferred to 250 mL flask in 100 mL 293 Freestyle medium (Gibco) supplemented with G418 and cultured in shaking incubator at 37°C with 5% CO2 ...
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bioRxiv - Immunology 2021Quote: ... 80 ml HyClone SFM4HEK293 medium (Cytiva) and 80 ml FreeStyle 293 Expression Medium (Thermo Fisher Scientific) were added to each 0.8 liter of cells ...
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bioRxiv - Genomics 2020Quote: ... Flp-In T-REx 293 cells were transduced and selected with 1 mg/mL Geneticin (Invitrogen) for six days ...
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bioRxiv - Immunology 2020Quote: ... 2.5×106 cells/ml were transfected using ExpiFectamine™ 293 transfection reagent (ThermoFisher, cat. no. A14524) as per manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: HEK293 cells (Flp-In 293 T-REx cell lines) were obtained from Life Technologies (R780-07) (Invitrogen) ...
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bioRxiv - Immunology 2021Quote: ... Plasmids encoding Spike-2P and HexaPro 61 were transiently transfected in FreeStyle 293 cells (Thermo Fisher) using Turbo293 (SpeedBiosystems ...
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bioRxiv - Immunology 2020Quote: ... and grown at 37°C in Freestyle™ 293 Expression Medium (Thermo Fisher Scientific, catalog #12338002) in baffle-bottomed flasks orbiting at 135 rpm ...
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bioRxiv - Molecular Biology 2022Quote: ... and 293 Flp-In cells were grown in Dulbecco’s Modified Eagle Medium (DMEM; Gibco/Thermo Fisher) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Molecular Biology 2022Quote: ... and 293 Flp-In cells were grown in Dulbecco’s Modified Eagle Medium (DMEM; Gibco/Thermo Fisher) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Biochemistry 2022Quote: ... The stable cells were grown in suspension at 37 °C in FreeStyle 293 Expression Medium (ThermoFisher). When the cells reached a density of 2.0–2.5 × 106 cells per ml ...
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bioRxiv - Immunology 2022Quote: ... and 2.7 μL/mL of cells Expifectamine 293 reagent (in Thermo Fisher Scientific kit, cat. # A14524) mixing 3 to 5 times by pipetting and incubating at RT for 20 to 30 min ...
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bioRxiv - Microbiology 2022Quote: ... The plasmid was transiently transfected into Expi293F cells using ExpiFectamine™ 293 Reagent (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... The transfection mix was replaced after 6 hours by 293 SFM II suspension medium (Invitrogen, 11686029), supplemented with sodium bicarbonate (3.7 g/L) ...