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Citations for Thermo Fisher :
1951 - 2000 of 10000+ citations for TIM 3 Human HEK 293 Fc His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... supplemented with 10% heat-inactivated (HI)-FBS (Gibco, 10438-026), 100 ng/mL IL21 (Gibco ...
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bioRxiv - Cell Biology 2019Quote: ... All PCR reactions were performed using Hi-Fi Taq (Invitrogen) or Herculase II Fusion (Agilent ...
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bioRxiv - Cell Biology 2020Quote: ... All PCR reactions were performed using Hi-Fi Taq (Invitrogen) with appropriate primers.
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bioRxiv - Microbiology 2021Quote: ... 10% heat inactivated fetal bovine serum (HI-FBS; Life Technologies) and 1X penicillin/streptomycin (pen/strep ...
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bioRxiv - Immunology 2020Quote: ... 1 mg of magnetic bead (Dynabeads™ His-Tag, Invitrogen) were coated with 70 μg of histidine tagged RBD ...
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Rac1, Rac3 GTPases and TPC2 are required for axonal outgrowth and migration of cortical interneuronsbioRxiv - Neuroscience 2022Quote: ... The Ion PI Hi-Q Sequencing 200 kit (Life Technologies) was used for all sequencing reactions and Torrent Suite™ Software 5.0 (Life Technologies ...
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bioRxiv - Plant Biology 2022Quote: ... secondary anti-6x-His tag monoclonal antibody (Invitrogen, # MA1-21315) at 1:175 dilutions ...
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bioRxiv - Microbiology 2023Quote: ... The PCR products were cloned into pcDNA3.1/Myc-His(+) (Invitrogen). Full-length sequences of DBA and 129 IFI207 were obtained from the cloned cDNAs ...
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bioRxiv - Cancer Biology 2022Quote: ... Blue Mix Hi-ROX (PCR Biosystems) and StepOnePlus (Applied Biosystems). The data expressed as the ratio between the expression level of IL-6 mRNA and that of Actin ...
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bioRxiv - Cell Biology 2024Quote: ... The anti-HIS antibody (MA1 21315) was purchased from Invitrogen.
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bioRxiv - Bioengineering 2024Quote: Nitrocellulose transfers were stained with anti-His (Invitrogen PA1-983B), anti-SARS-CoV-S (Absolute Antibodies CR3022) ...
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bioRxiv - Biophysics 2024Quote: 6x-His epitope tag antibody (His.H8) was purchased from Invitrogen. Monoclonal Flag M2 antibody (F1804-50UG) ...
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bioRxiv - Biophysics 2024Quote: ... 7 mL of His-Pur Ni-NTA resin (Thermo Scientific) was equilibrated in Buffer A containing 40 mM imidazole ...
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bioRxiv - Molecular Biology 2024Quote: ... and incubated with a 6x-His-Tag monoclonal antibody (Invitrogen) diluted 2500-fold in PBS-Tween overnight ...
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bioRxiv - Molecular Biology 2022Quote: ... and cloned it in the pcDNA3.1/myc-His(-) plasmid (Invitrogen) in frame with a Myc-His tag to obtain the wild-type (WT ...
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bioRxiv - Neuroscience 2023Quote: ... 6x-His Tag Monoclonal Antibody (HIS.H8) (MA1-21315, Thermo Scientific) and HRP-conjugated goat anti-mouse secondary antibody (Abcam ab6789-1 MG ...
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bioRxiv - Molecular Biology 2023Quote: ... with 5% heat inactivated Fetal bovine serum (HI-FBS) (Gibco). 1 million cells per 100 μl per sample were stained with anti-Flag antibody (Abcam ...
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bioRxiv - Microbiology 2023Quote: ... protein expression from plasmid (derivates of pBAD-His/B (Invitrogen) in all cases except expression of VirF from pCL5 [71] ...
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bioRxiv - Biochemistry 2022Quote: ... 1 nM LanthaScreen Elite Tb-anti-His antibody (ThermoFisher #PV5895), and 400 nM FITC-labeled PGC1α peptide in a buffer containing 20 mM potassium phosphate (pH 7.4) ...
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bioRxiv - Biophysics 2023Quote: ... The medium was supplemented with 10% HI-FBS (Gibco, USA), 2 mM L-glutamine ...
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bioRxiv - Biochemistry 2023Quote: ... bio-His-PADI4 was incubated with magnetic streptavidin beads (Invitrogen) (4 °C ...
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bioRxiv - Neuroscience 2024Quote: ... and 10% fetal bovine serum (Gibco FBS HI, Cat# A5256701) in a CO2 incubation at 37 °C ...
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bioRxiv - Immunology 2024Quote: ... The PCR products were cloned into pcDNA3.1/Myc-His(+) (Invitrogen). Full-length sequences of DBA and 129 IFI207 were obtained from the cloned cDNAs ...
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bioRxiv - Cell Biology 2023Quote: Monkey kidney epithelial cells Vero E6 (clone CCL81) and human lung epithelial cells Calu-3 were respectively cultured in DMEM medium and EMEM medium (ThermoFisher) supplemented with 10% heat-inactivated fetal bovine serum (FBS) ...
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bioRxiv - Cell Biology 2023Quote: ... RPE-1 cells or MiniBAR-GFP stable cell lines were transfected with 25 nM of siRNAs targeting either luciferase or human Mini-BAR (5’-CUGCAAAUUUUACGGAUCA-3’) using Lipo-fectamine RNAiMAX (Invitrogen), following the manufac-turer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: HEK293T cells (human female in origin) were cultured every 3-4 days using 0.05% Trypsin EDTA and maintained in DMEM (Gibco, 11965118) supplemented with 10% heat inactivated fetal bovine serum (iFBS ...
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bioRxiv - Immunology 2023Quote: ... Purified T cells were activated for 1 to 3 days with Human T-Activator anti-CD3/CD28 dynabeads at a 1:2 bead-to-cell ratio (Gibco, Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2024Quote: ... and human (goat anti-human IgG, ThermoFisher) were also used ...
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bioRxiv - Microbiology 2020Quote: ... All constructs were transfected into Expi293 cells using ExpiFectamine(tm) 293 Transfection Kit (Thermo Fisher). Antibodies were purified by immobilized metal chelate affinity chromatography using nickel-nitrilotriacetic acid (Ni-NTA ...
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bioRxiv - Molecular Biology 2020Quote: ... and stable FlpIn 293 T-REx cell lines were maintained with hygromycin (Life Technologies, 10687010) at 2ug/ml ...
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bioRxiv - Bioengineering 2022Quote: ... Suspension-adapted HEK293F cells were maintained in serum-free Free Style 293 Expression Medium (Gibco). On the day of transfection ...
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bioRxiv - Synthetic Biology 2019Quote: Gene circuits were introduced into Flp-In 293 cells (Thermo Fisher Scientific catalog number R75007) using lipofectamine 3000 (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2020Quote: ... suspension adapted HEK293GnTI- cells growing in freestyle 293 expression media supplemented with 2 % FBS (Gibco), 2 mM Glutamine (Gibco ...
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bioRxiv - Synthetic Biology 2021Quote: FreeStyle 293-F and FreeStyle CHO-S cell lines were obtained from Invitrogen (Carlsbad, CA) and cultured in FreeStyle 293 Expression Medium and complete FreeStyle CHO Expression Medium (Invitrogen) ...
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bioRxiv - Synthetic Biology 2021Quote: ... CA) and cultured in FreeStyle 293 Expression Medium and complete FreeStyle CHO Expression Medium (Invitrogen), respectively ...
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bioRxiv - Biochemistry 2021Quote: ... The recombinant IgG (XBA14 and XBA18) was produced in FreeStyle 293 cells (Thermo Fisher Scientific), and purified by Protein A-Sepharose (Cytiva ...
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bioRxiv - Molecular Biology 2022Quote: ... and 100 μg/ml streptomycin or the GripTite 293 MSR Cell Line (Thermo Fisher Scientific) (HEK-GT ...
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bioRxiv - Bioengineering 2022Quote: ... was expressed in an Expi293F cell system with an ExpiFectamine 293 Transfection Kit (ThermoFisher, USA) according to the manufacturer’s protocol and purified as described previously 45.The ACE2 or DnbALFA proteins were labeled by CF®640R succinimidyl ester dye (Biotium ...
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bioRxiv - Microbiology 2021Quote: ... was transfected into 293 LTV cells for lentivirus production using Lipofectamine 2000 (Thermo Fisher Scientific). The supernatant was collected 48 hours post transfection and clarified by centrifugation (3500 rcf ...
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bioRxiv - Molecular Biology 2019Quote: ... All experiments were performed in the Flp-In T-REx 293 cell line (Thermo Scientific). The mCherry and mEGFP versions of RFP and GFP are used throughout this manuscript ...
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bioRxiv - Cancer Biology 2019Quote: The Flp-In™ T-REx™ 293 Cell Line was procured from Thermo Fisher Scientific (R78007 ...
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bioRxiv - Biochemistry 2021Quote: ... The SARS-CoV-2 spike glycoprotein RBD was expressed in Freestyle 293 cells (ThermoFisher, MA) grown in Freestyle 293 media at 37 °C with 8% CO2 and shaking at 135 rpm in a humidified incubator ...
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bioRxiv - Neuroscience 2021Quote: ... cells were incubated with 25µL of odorant solution diluted in CD-293 (Gibco 11913-019) containing 30 µM CuCl2 (Sigma-Aldrich C-6641 ...
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bioRxiv - Genetics 2021Quote: ... Flp-In T-REx 293 cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM) (Gibco) and supplemented with 10% Fetalgro® Bovine Growth Serum (BGS) ...
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bioRxiv - Genomics 2020Quote: ... Flp-In T-REx 293 cells were also supplemented with 100 μg/ml Zeocin (Invitrogen). Cells were passaged every 2-4 days ...
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bioRxiv - Immunology 2020Quote: ... Cells were maintained at 37°C and 6% CO2 FreeStyle 293 Expression Medium (Thermo Fisher) and 0.2% Penicillin/Streptomycin 7 days post transfection ...
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bioRxiv - Microbiology 2022Quote: ... The plasmids were transiently transfected into Expi293F cells using ExpiFectamine 293 Reagent (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... The transfection mix was replaced after 6 hours by 293 SFM II suspension medium (Invitrogen, 11686029 ...
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bioRxiv - Bioengineering 2024Quote: ... according to the manufacturer’s instructions and cultured in FreeStyle 293 Expression Medium (Thermo Fisher Scientific) in a humidified incubator with 8% CO2 at 37°C and 125 rpm ...
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bioRxiv - Immunology 2023Quote: ... All adenovirus vectors were produced in the T-REx-293 cell line (Thermo Fisher Scientific), which allows for transcriptional repression of the vaccine antigens during vector production ...