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Citations for Thermo Fisher :
2251 - 2300 of 5010 citations for Scyliorhinin II amide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2023Quote: ... CDS were then integrated into the pDONR207 plasmid using BP clonase II mix (Thermofisher). pDONR207 plasmids containing PpSMXL CDS were submitted to PCR-mediated mutagenesis to obtain ΔRGK/RT versions.
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Autophagy suppression in DNA damaged cells occurs through a newly identified p53-proteasome-LC3 axisbioRxiv - Cell Biology 2024Quote: 8-well cell culture chamber slides (Lab-Tek II; #154534, Thermo Fisher Scientific, USA) were analyzed using a DeltaVision RT microscope (GE Life Sciences ...
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bioRxiv - Cell Biology 2024Quote: ... cDNA was prepared using RnaseOUT and Superscript II according to the manufacturer’s protocol (Invitrogen). qPCRs were performed on the qTower 3 from Analytik Jena ...
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bioRxiv - Developmental Biology 2024Quote: ... Purified RNA was reverse-transcribed into cDNAs with SuperScript® II reverse transcriptase (Invitrogen) according to the manufacturer’s protocols ...
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bioRxiv - Molecular Biology 2024Quote: ... Sf9 cells were cultured in Sf-900™ II SFM (Thermo Fisher Scientific/Gibco) at 28°C on an orbital shaker set at 135 rpm ...
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bioRxiv - Biophysics 2024Quote: Sf9 cells for baculovirus generation were cultured in Sf-900 II SFM medium (GIBCO) supplemented with 5% FBS and 1% Antibiotic-Antimycotic ...
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bioRxiv - Bioengineering 2024Quote: ... PCR was conducted with Phire II Hot-Start PCR 2X mastermix (Thermo Scientific; F125L) using 100 ng of gDNA template ...
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bioRxiv - Bioengineering 2024Quote: ... Cell samples were counted using a Countess II Haemocytometer (Life Technologies, Carlsbad, CA, USA), and plated onto single wells of a 6-well tissue culture plate at a density of 50 cells/cm2 with 2 mL of cloning media ...
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bioRxiv - Cancer Biology 2024Quote: ... BM was digested for 30 minutes in HBSS containing 2mg/mL Dispase II (Gibco), 1mg/mL Collagenase Type IV (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2024Quote: ... together with the Phusion Hot Start II High-fidelity DNA Polymerase (Thermo Fisher Scientific) following the manufacturer’s recommendations ...
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bioRxiv - Developmental Biology 2024Quote: ... RNA was reverse transcribed to cDNA using the SuperScript II Reverse Transcriptase kit (Invitrogen). qPCRs were conducted using the SensiFAST SYBR No-ROX kit (Bioline ...
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bioRxiv - Microbiology 2024Quote: ... PCR reactions were performed using Platinum™ SuperFi II DNA Polymerase (ThermoFisher Scientific: 12361010) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... Sf9 cells were cultured in Sf-900™ II SFM (Thermo Fisher Scientific/Gibco) at 28°C on an orbital shaker set at 135 rpm ...
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bioRxiv - Molecular Biology 2024Quote: ... Purified amplicons were cloned into pDONR/Zeo via Gateway BP Clonase II (Thermo Fisher) and transformed into E ...
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bioRxiv - Biochemistry 2024Quote: ... while shuttling into destination vectors was performed with Gateway LR Clonase II (ThermoFisher Scientific) and positive clones were confirmed by colony PCR.
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... The PCR reactions were conducted using TaqMan Universal Master Mix II (4440040, Applied Biosystems) and 20× TaqMan microRNA Assays (4427975 and 4440886 ...
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bioRxiv - Genetics 2024Quote: ... Monocyte number and viability was assessed using Countess II Automated Cell counter (Thermo Fisher). After sorting ...
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Tuning the performance of a TphR-based terephthalate biosensor with a design of experiments approachbioRxiv - Synthetic Biology 2024Quote: ... and DNA amplification for colony PCR was performed using Phire II Green (Thermo Fisher). The pSEVA131 plasmid was used as the backbone for the generation of all biosensor constructs ...
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bioRxiv - Immunology 2024Quote: ... qPCR reactions were performed with TaqMan™ Universal Master Mix II (Applied Biosystems™). Gene expression was quantified by QuantStudio™ 3 or 12K Flex Real-Time PCR Systems (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were counted on Countess II automated cell counter (Thermo Fisher, Waltham, Massachusetts, USA), and up to 12,000 cells were loaded per lane on 10x Chromium microfluidic chips (10x Genomics ...
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bioRxiv - Developmental Biology 2024Quote: ... was employed to infect Sf9 cells cultured in Sf-900TM II SFM medium (Gibco) and at 27 °C ...
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bioRxiv - Bioengineering 2020Quote: ... PCR templates were amplified with Phusion Hot Start II High-Fidelity DNA Polymerase (Thermo Scientific) and amplified products were purified with PureLink Quick PCR Purification Kit (Invitrogen ...
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bioRxiv - Microbiology 2020Quote: ... all PCR products were ligated into the pCR®-Blunt II-TOPO® Vector (Invitrogen) according to the manufacturer’s recommendation.
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bioRxiv - Cancer Biology 2021Quote: ... were used for construct generation by an LR reaction with LR Clonase II Plus (Invitrogen) (Kwan et al. ...
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bioRxiv - Cancer Biology 2021Quote: ... and CRKL to PLX304 with the Gateway LR Clonase II Enzyme mix (Thermo Fisher 11791020). The identity of each vector was validated by targeted sequencing ...
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bioRxiv - Cell Biology 2019Quote: ... The pelleted cells were lysed using B-PER II bacterial protein extraction reagent (Thermo Fisher) for 15 min ...
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bioRxiv - Cell Biology 2019Quote: ... 100 ng of RNA was transcribed into cDNA using SuperScript II reverse transcriptase (ThermoFisher Scientific) with random hexamer primers (Invitrogen) ...
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bioRxiv - Genetics 2021Quote: ... which was mediated by a Gateway reaction using BP clonase II (Thermo Fisher Scientific, #11789100) following a PCR reaction and addition of attB sites to the amplicon (Harnish et al. ...
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bioRxiv - Developmental Biology 2021Quote: ... DNA from F1 heterozygotes was cloned into pCR™Blunt II-TOPO® vector (Thermofisher) and sequenced ...
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bioRxiv - Developmental Biology 2021Quote: ... Cell concentration and viability were assessed using the Countess II automated cell counter (Applied Biosystems). The samples with final concentration of more than 650 cells/µL and viability of more than 85% were used for single cell RNA-Seq.
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bioRxiv - Developmental Biology 2021Quote: ... and digested into single-cell suspensions with 1 mg/ml type II collagenase (17101015, GIBCO) and 1 mg/ml type IV collagenase (17104019 ...
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bioRxiv - Developmental Biology 2021Quote: ... RNA was reverse transcribed to cDNA using the SuperScript™ II Reverse Transcriptase Kit (Invitrogen). qPCR reactions were carried out using the SensiFAST™ SYBR® No-ROX Kit (Bioline ...
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bioRxiv - Molecular Biology 2020Quote: ... All reactions were stopped with 5 μL Gel Loading Buffer II (Life Technologies, Cat # AM8547). Digested DNA was incubated at 75°C for 5 min before running on 4% (Nt.BspQI-digested samples ...
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bioRxiv - Molecular Biology 2021Quote: ... First strand cDNA synthesis is performed using SuperScript II Reverse Transcriptase (Invitrogen, Carlsbad, CA, USA) and random primers ...
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bioRxiv - Evolutionary Biology 2020Quote: ... measured using a Qubit 3.0 fluorometer (Invitrogen).RNA was chemically fragmented and first strand cDNA generated using SuperScript II (Thermo Fisher Scientific) as per manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2020Quote: ... The amplicon was then TA-cloned into a dual promoter pCR TOPO-II vector (ThermoFisher). To ensure we had the full-length transcript we performed 3’RACE (SMARTer RACE Takara ...
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bioRxiv - Developmental Biology 2020Quote: ... Proteins were transferred to the membranes using XCell II™ Blot Module (ThermoFisher Scientific, USA) at 4°C ...
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bioRxiv - Developmental Biology 2022Quote: ... The 257-bp PCR amplicons were amplified using Phusion Hot Start II (Thermo Fisher Scientific) and sequenced for genotyping.
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bioRxiv - Molecular Biology 2021Quote: ... The effluent was then directed to an electrospray ion source (Thermo Fisher Scientific, HESI-II) connected to a triple quadrupole mass spectrometer (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2020Quote: ... and the PCR product was inserted into pCR-Blunt II-TOPO vector (Thermo Fisher Scientific). Furthermore ...
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Single-cell analysis of skeletal muscle macrophages reveals age-associated functional subpopulationsbioRxiv - Cell Biology 2022Quote: ... the muscle slurry was digested with 1000 U/mL Collagenase type II (Gibco, Cat# 17101015) in 10 mL of complete Ham’s F-10 medium (Lonza ...
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bioRxiv - Cell Biology 2022Quote: 1,5 × 106 cells/wells were transfected with 5 μg pDNA using Cellfectin II reagent (Gibco) according to the manufacturers protocol.
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bioRxiv - Immunology 2022Quote: ... and cDNA was synthesized using the Superscript II First-Strand Synthesis kit (Invitrogen, Carlsbad, CA). SYBR Green qPCR analysis was performed using the CFX Connect Real-Time system (Bio-Rad ...
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bioRxiv - Genomics 2020Quote: ... and further diluted to 300 cells / µl after cell count (Countess II FL, Life Technologies). For the 5% spike-in and 10% spike-in cell mixtures ...
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bioRxiv - Microbiology 2020Quote: ... were removed from the final pool using a 2% E-gel size-select II (ThermoFisher). Quality of the pool was assessed with the Bioanalyzer DNA 1000 chip (Agilent Technologies ...
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bioRxiv - Cancer Biology 2019Quote: ... cry:eGFP constructs were generated by MultiSite Gateway assemblies using LR Clonase II Plus (Life Technologies) according to standard protocols and Tol2kit vectors described previously61 ...
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bioRxiv - Evolutionary Biology 2019Quote: ... and transferred to pUBN-GFP-DEST using the GatewayTM LR Clonase II Enzyme Mix (Invitrogen).
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bioRxiv - Developmental Biology 2019Quote: ... and reverse transcription was performed using an oligoDT primer using superscript II (Thermofisher, Cat#18064014). QPCR was performed on a 7900HT Fast Real-Time PCR System (Thermofischer ...
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bioRxiv - Bioengineering 2019Quote: ... were measured using Countess II automated cell counter or trypan blue (Fisher Scientific, Waltham, MA). All basal media ...
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bioRxiv - Biochemistry 2019Quote: ... copied to cDNA using an Oligo dT and the SuperScript™ II Reverse Transcriptase (Invitrogen). RT-PCR was performed with primers RcCHAD_RT_F (5-ATTGCCCAGGCAAAGCGTCATGC-3 ...