Labshake search
Citations for Thermo Fisher :
2101 - 2150 of 10000+ citations for Human Immunodeficiency Virus Tat Protein HIV 1 Clade D UG28R since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Plant Biology 2022Quote: ... was transcribed following DNase I treatment using Moloney Murine Leukemia Virus reverse transcriptase and an oligo dT(18) primer according to the manufacturer’s protocol (ThermoFisher Scientific). qPCR analysis was carried out in a CFX96-C1000 96-well plate thermocycler (Bio-Rad ...
-
bioRxiv - Immunology 2022Quote: ... 5% CO2 until the following day. Virus stocks were diluted into supplemented media (DMEM from Gibco, Cat. no. 61965026 supplemented with 2% FBS (Gibco, Cat ...
-
bioRxiv - Neuroscience 2022Quote: ... 1 µg of total RNA was subjected to random reverse transcription using Moloney Murine Leukemia Virus reverse transcriptase (M-MLV-RT; Invitrogen) and random primers (Roche) ...
-
bioRxiv - Microbiology 2023Quote: ... generated by Knut Falk in the late 1990s) or mouse IgG2a targeting influenza virus NP (clone D67J, Thermo Fisher Scientific) (both ...
-
bioRxiv - Developmental Biology 2023Quote: ... PD2 and TD1 hiPSC were generated from deidentified commercially available human peripheral blood mononuclear cell and tracheal epithelial cell lines via the manufacturing protocol of Sendai virus-mediated reprogramming (CytoTune2.0) (ThermoFisher, A16517). Every other month ...
-
bioRxiv - Synthetic Biology 2023Quote: ... The virus concentration using Lenti-X Concentrator was performed with the manufacturer’s protocol and dissolved in Opti-MEM (Gibco #31985062) to yield a 10- or 15-fold concentration ...
-
bioRxiv - Microbiology 2023Quote: BmN cells infected with WT virus at indicated time were harvested and total RNA was extracted by using TRIzol reagent (Invitrogen). 2 μg total RNA was prepared to synthesis the cDNA by TransScript One-Step gDNA Removal and cDNA Synthesis SuperMix (TransGen) ...
-
bioRxiv - Microbiology 2023Quote: ... The following day we washed cells with DPBS twice and added half-log10 dilutions of the virus sample in infection media (MEM medium (Gibco) containing 0.3% BSA (fraction V ...
-
bioRxiv - Microbiology 2023Quote: Influenza A virus (IAV) strain A/WSN/33 [H1N1 subtype] was propagated in Madin-Darby Canine Kidney (MDCK) cells (ThermoFisher). Cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM ...
-
bioRxiv - Microbiology 2023Quote: ... Lungs from K18-ACE2 C57BL/6 mice infected with virus were collected at indicated time points and were homogenized in Trizol (Invitrogen) and RNA was isolated using manufacturer’s instructions ...
-
bioRxiv - Pathology 2023Quote: ... Total RNA was extracted from clinical specimens (swabs and tissues) and virus isolates using the MagMax AM1836 96 Viral RNA Isolation Kit (ThermoFisher Scientific ...
-
bioRxiv - Genetics 2024Quote: ... The iPSCs were made Sendai virus free by keeping in culture and testing for the presence of Sendai Virus (SeV) at different times by measuring SeV levels in total RNA using RT-qPCR with FAM labeled SeV (Thermofisher Scientific ...
-
bioRxiv - Microbiology 2024Quote: ... cDNA of the virus genome was generated by reverse transcription with random hexamer as the primer (SuperScript III, ThermoFisher Scientific). The HA1 subunit was divided into three fragments for PCR (PrimeSTAR Max DNA Polymerase ...
-
bioRxiv - Biochemistry 2023Quote: ... Concentrated virus was applied through a 0.45 μm surfactant free cellulose acetate syringe filter (Nalgene, Fisher Scientific #09-740-106) and immediately used or stored at -80ºC for up to one month ...
-
bioRxiv - Systems Biology 2024Quote: ... 1 million 293T STING-mNG or 0.5 million BJ1 seeded the same day in one well of a 6 well plate were transduced with 2ml of virus including 8µg/ml Protamine (Thermo Fisher). Cells were incubated with viral supernatant for 1-2 days and cells were then transferred in a fresh flask for selection ...
-
bioRxiv - Genomics 2024Quote: ... the fibroblasts were thawed and plated at a density of 2.5×105 cells/well of 6-well plate and infected with the Cytotune Sendai virus (Life Technologies) per manufacturer’s protocol to initiate reprogramming ...
-
bioRxiv - Developmental Biology 2024Quote: ... A total of 1 µg of RNA was used as a template for cDNA synthesis using Moloney Murine Leukemia Virus reverse transcriptase (M-MLV RT) (Invitrogen).
-
bioRxiv - Cell Biology 2024Quote: ... GH3 cells were transduced with supernatants containing virus carrying the shMeCP2 or shhnRNP L construct using the Lipofectamine 3000 reagent (Invitrogen). After 48 h ...
-
bioRxiv - Neuroscience 2024Quote: ... PBMCs were reprogrammed into iPS cells using transient overexpression of pluripotency-associated factors (OCT4, KLF4, and SOX2) via non-integrating Sendai virus particles (Cytotune 2.0 kit, Life Technologies). At least five clones per TSC2+/+ and TSC2+/− donor were established into iPS cell lines ...
-
bioRxiv - Cancer Biology 2022Quote: ... Antibodies used for human cells are mouse anti-human CD3 conjugated to APC (Thermo Fisher, 47-0036-42), mouse anti-human CD8 conjugated to FITC (BD Biosciences ...
-
bioRxiv - Cell Biology 2022Quote: Human cerebral organoids are generated from human induced pluripotent stem cells (iPSCs) which were purchased from GibcoTM (Gibco by Thermofisher ...
-
bioRxiv - Cancer Biology 2020Quote: ... anti-human TNF-α (hTNF-α)-PE and anti-human IFN-ɣ (hIFN-ɣ)-PECy7 were from ThermoFisher. Anti-human CD4 (hCD4)-BV711 was from Biolegend.
-
bioRxiv - Immunology 2020Quote: ... using a secondary antibody recognizing all human immunoglobulin isotypes (goat anti-human IgG H+L-HRP, Invitrogen/ThermoFisher) [12,13] ...
-
bioRxiv - Immunology 2021Quote: ... which contains the gene expression profile of human microglia using a human gene ST 2.0 Microarray Chip (Affymetrix). The human microglia were polarized to either pro-inflammatory (M1 ...
-
bioRxiv - Immunology 2020Quote: ... using a secondary antibody recognizing all human immunoglobulin isotypes (goat anti-human IgG H+L-HRP, Invitrogen/ThermoFisher) [12,13] ...
-
bioRxiv - Immunology 2023Quote: Total human IgG levels were determined following the manufacturer’s instructions in the Human IgG Total ELISA Kit (Invitrogen).
-
bioRxiv - Bioengineering 2023Quote: ... primary human T cells were thawed and activated with anti-CD3/CD28 Human T-Expander Dynabeads (Thermo Fisher) at a 3:1 bead to cell ratio ...
-
bioRxiv - Synthetic Biology 2023Quote: ... primary human T cells were thawed and activated with anti-CD3/CD28 Human T-Expander Dynabeads (Thermo Fisher) at a 3:1 bead to cell ratio ...
-
bioRxiv - Neuroscience 2024Quote: ... The concentrations of human α-synuclein were measured using a human α-syn-specific ELISA (ThermoFisher Scientific #KHB0061) according to the manufacturer’s protocol ...
-
bioRxiv - Developmental Biology 2024Quote: ... Human full-coding KATNA1 cDNA was isolated from a human fetal brain Marathon-ready cDNA collection (Clontech, Invitrogen) with the following forward and reverse primers ...
-
bioRxiv - Molecular Biology 2021Quote: ... Protein A-tagged proteins were detected with HRP-conjugated polyclonal anti-Protein A (Invitrogen, PA1-26853) at 1:5000.
-
bioRxiv - Developmental Biology 2022Quote: ... Proteins were resolved alongside a protein ladder (PageRuler Plus Pre-stained Protein Ladder, Thermo Fisher Scientific) on NuPAGE 4-12% BisTris gradient gels using NuPAGE MOPS SDS running buffer (Thermo Fisher Scientific) ...
-
bioRxiv - Cell Biology 2023Quote: ... Protein concentration was quantified using Qubit™ Protein and Protein Broad Range (BR) Assay Kits (ThermoFisher).
-
bioRxiv - Microbiology 2023Quote: ... and protein concentration was determined by protein assay (EZQ Protein Quantification Kit; Life Technologies, Carlsbad, CA). Ct226-FLAG was affinity purified using magnetic FLAG beads (Sigma-Aldrich ...
-
bioRxiv - Microbiology 2020Quote: ... The enzymatic activity of Ddl was also assayed by measuring the release of inorganic phosphate in the reaction (similar to the D-ala-D-ala dipeptide formation assay) with EnzChek phosphate assay kit (Life Technologies). 10 μl of the reaction product was added to 790 μl of standard reaction mixture containing 230 μl dH2O ...
-
bioRxiv - Plant Biology 2022Quote: ... 2006) and the cassettes cloned into pENTR/D-TOPO using a pENTR™/D-TOPO™ Cloning Kit (Thermo Fisher Scientific) and subsequently pUB-DEST (Grefen et al. ...
-
bioRxiv - Cell Biology 2020Quote: ... Primary antibodies included rabbit polyclonal anti-β-COP protein (1:200; Thermo Fisher, PA1-061) and mouse monoclonal anti-flag (1:500 ...
-
bioRxiv - Pathology 2019Quote: ... Proteins were stained with 1-Step™ Ultra TMB-ELISA Substrate Solution (Thermo Fisher Scientific) until blue colour was fully generated and the reaction stopped by adding 1 M H2SO4 ...
-
bioRxiv - Microbiology 2019Quote: ... Labeled proteins were visualized with Alexa Fluor-conjugated secondary goat antibodies (1:2000, Life Technologies). Nuclei were visualized with the DNA stain (DAPI ...
-
bioRxiv - Cell Biology 2019Quote: ... 100 μM of the protein was incubated with 1 mM tris(2-carboxyethyl)phosphine (Invitrogen) for 30 min at RT protected from light ...
-
bioRxiv - Bioengineering 2021Quote: ... 1 µL of 1M DTT and 2.5 µL of 4x LDS protein loading buffer (Invitrogen) were added to the bead mixture ...
-
bioRxiv - Bioengineering 2021Quote: ... STEMdiff APEL2 medium (1% (v/v) PFHM-II protein-free hybridoma medium (Thermo Fisher Scientific) and 1% (v/v ...
-
bioRxiv - Biochemistry 2021Quote: ... The substrate proteins were modified with a 1:0.8 ratio Alexa Fluor 680 (Thermo Fisher) for 10 min and quenched with 5 mM DTT ...
-
bioRxiv - Cancer Biology 2021Quote: ... Primary antibodies used were PON1 (18155-1-AP, Protein tech) and PON3 (OTI1A5, Thermo Scientific).
-
bioRxiv - Bioengineering 2020Quote: ... rabbit anti-S100 Protein Ab-2 (S100, 1:500, Thermo Scientific Cat# RB-044-A) was used to identify Schwann cells ...
-
bioRxiv - Microbiology 2021Quote: ... MeV-M proteins were probed using rabbit anti-EGFP polyclonal antibody (1:1000) (Invitrogen #A6455). The Venus fluorescent protein used in BiFC experiments is a genetic variant of EGFP ...
-
bioRxiv - Cell Biology 2021Quote: ... The proteins were probed with the following antibodies: Monoclonal anti-GFP (1:2000, Thermo Scientific), anti-β-actin (1:2000 ...
-
bioRxiv - Molecular Biology 2022Quote: ... the supernatant was incubated with 1/14 volume of free Protein G Dynabeads (ThermoFisher Scientific) rolling for 1 hour at 5°C to remove sticky proteins and RNAs ...
-
bioRxiv - Genetics 2022Quote: ... PDE6B protein was detected by Rabbit anti-PDE6B antibody (1:500; Thermo Scientific, PA1-722). Mouse anti-GAPDH antibody (1:10000 ...
-
bioRxiv - Cell Biology 2022Quote: ... at a dilution of 1:1,000 and the cytoplasmic protein GAPDH (Thermo Fisher Scientific, AM4300) at a dilution of 1:5,000) ...