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Citations for Thermo Fisher :
2001 - 2050 of 10000+ citations for Human Immunodeficiency Virus Tat Protein HIV 1 Clade D UG28R since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: Cas9 was produced by IVT using either a continuous exchange 1-Step Human Coupled IVT Kit (Thermo Fisher Scientific) or a PURExpress bacterial IVT kit (New England Biolabs) ...
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bioRxiv - Microbiology 2021Quote: ... Cells were then washed once with PBS and incubated with 1 ug/ml anti-human (Alexa Fluor 647; Invitrogen) secondary Abs and the viability dye AquaVivid (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2020Quote: ... Samples were further diluted 1:100 in standard diluent and Aβ levels were measured with the human Aβ1–42 ELISA kit (Invitrogen) following the manufacturer’s instructions.
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bioRxiv - Immunology 2021Quote: ... The human myelomonocytic leukemia cell line THP-1 was propagated in RPMI 1640 medium (GIBCO® Life Technologies™) supplemented with 10% fetal calf serum (FCS ...
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bioRxiv - Neuroscience 2019Quote: ... for 1 h at room temperature and then exposed to antibodies specific for human LMP7 (ThermoFisher Scientific; MA5-15890) or PRDX6 (Abcam ...
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bioRxiv - Cell Biology 2021Quote: Total RNA (1 μg) preparations were hybridized on the PrimeView Human Gene Expression Array (Affymetrix, Thermo Fisher Scientific, USA) at the core facility Biomedizinisches Forschungszentrum (BMFZ ...
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bioRxiv - Cell Biology 2021Quote: Total RNA (1 μg) preparations were hybridized on the PrimeView Human Gene Expression Array (Affymetrix, Thermo Fisher Scientific, USA) at the core facility Biomedizinisches Forschungszentrum (BMFZ ...
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bioRxiv - Cell Biology 2021Quote: ... The following secondary antibodies were used at 1:200 for IF experiments: Anti-human-Alexa-633 (Life Technologies #A21091), anti-mouse-Alexa-488 (Life Technologies #A11029) ...
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bioRxiv - Molecular Biology 2022Quote: Human Embryonic Kidney (HEK293T) cells were cultured in high glucose DMEM with 10% FBS and 1% Penicillin/Streptomycin (Gibco) in a 37°C incubator with 5% CO2 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: 1 mL of human plasma was incubated with 0.5 mL of Invitrogen total exosome isolation reagent (Thermo Fisher Scientific) overnight at 4°C and the day after was centrifuged at 10,000 x g for 1h at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... Alpha-actinin-1-M-sstFRET containing the sstFRET module inserted between 300aa and 301aa within the first spectrin repeat of human alpha-actinin-1 (P12814.2) was in the neomyocin selectable pcDNA3.1 expression vector (Invitrogen/ThermoFisher). Actinin-C-sstFRET containing the sstFRET module added to the C-terminus of human alpha-actinin-1 (P12814.2 ...
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bioRxiv - Microbiology 2021Quote: ... falciparum NF54 parasites were cultivated at 4% hematocrit in human B+ blood in RPMI-HEPES supplemented with 0.25% Albumax 1 (Invitrogen) and human donor plasma type B+ ...
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bioRxiv - Synthetic Biology 2020Quote: ... and 1 day prior to spinfection cells were activated using Human T-activator CD3/CD28 Dynabeads (Thermo Fisher, 11131D) and cultured in 200U/ml IL-2 ...
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bioRxiv - Bioengineering 2021Quote: ... Another subset of slides was labeled with Alexa 568 conjugated anti-human IgG antibody (1:800, ThermoFisher, A-21090) to detect the Fc portion of the EphA4-Fc chimera peptide.
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bioRxiv - Immunology 2020Quote: Human PBMCs were incubated with MitoSOX Red (5μM) and Mitotracker Green (1 μM) (both from Molecular Probes, Life Technologies) in PBS during 10 minutes at 37°C before flow cytometry extra-cellular staining.
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bioRxiv - Immunology 2020Quote: Human PBMCs were incubated with MitoSOX Red (5μM) and Mitotracker Green (1 μM) (both from Molecular Probes, Life Technologies) in PBS during 10 minutes at 37°C before flow cytometry extra-cellular staining.
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bioRxiv - Immunology 2021Quote: ... The human myelomonocytic leukemia cell line THP-1 was propagated in RPMI 1640 medium (GIBCO® Life Technologies™) supplemented with 10% fetal calf serum (FCS ...
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bioRxiv - Biochemistry 2019Quote: ... The THP-1 (human monocytic cells) cells were grown on Roswell Park Memorial Institute (RPMI) medium (Gibco, CA, USA) supplemented with 10% FBS and 0.05 mM β-mercaptoethanol (Sigma-Aldrich ...
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bioRxiv - Bioengineering 2021Quote: ... Briefly, THP-1 human monocytes (ATCC®, Manassas, VA) were cultured in complete RPMI media (ThermoFisher Scientific, Waltham, MA), supplemented with 10% heat-inactivated FBS and 1% penicillin/streptomycin (37) ...
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bioRxiv - Cell Biology 2021Quote: ... for 1 h before opsonization with human IgG or 200 µg/mL anti-BSA antibody (Invitrogen, Burlington, ON, Canada). RAW cells were pre-cooled to 4°C for 5 min prior to the cells being presented with targets at ratios of 20-400 rods per cell ...
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bioRxiv - Immunology 2020Quote: ... the fixed sample was incubated with 1:500 dilution of goat anti-human coupled to Alexa 633 fluorophore (Invitrogen) and incubated for 30 min at RT ...
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bioRxiv - Immunology 2022Quote: ... Human THP-1 monocytes (ATCC, TIB-202) were grown in RPMI 1640 Medium with ATCC modification (Thermo Fisher Scientific) supplemented with 10% FBS and 50 μM 2-mercaptoethanol (Millipore Sigma) ...
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bioRxiv - Cell Biology 2022Quote: Large-scale production of bio-inactive human N-SLIT2ΔD2 was performed by transfecting HEK293F cells with human N-SLIT2ΔD2 cDNA (1 ug/mL) using PEI reagent (Polyethyleneimine, linear, M.W. 25,000, Thermo Fisher Scientific) (19) ...
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bioRxiv - Immunology 2022Quote: ... Wells were then incubated with a secondary goat anti-human IgG labeled with horseradish peroxidase (HRP) (1:5000 Invitrogen) TMB substrate (SIGMA) ...
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Alpha-1-antitrypsin binds to the glucocorticoid receptor with biological significance in macrophagesbioRxiv - Immunology 2022Quote: Human THP-1 cells were cultured in RPMI-1640 medium containing 2 mM L-glutamine (Gibco; Grand Island, NY), 10% FBS ...
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bioRxiv - Molecular Biology 2023Quote: ... Human β-arrestin 2 (residues 1-393, with I386A, V387A, and F388A mutations) was cloned into pBiT2.1 vector (Invitrogen) with the SmBiT at its N-terminus ...
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bioRxiv - Immunology 2023Quote: ... Cells were then incubated at 37 °C for 1 hour followed by adding anti-human Alexa Fluor-647 (Invitrogen) secondary Abs for 20 minutes ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: The human erythroleukemia cell line TF-1 (ATCC, USA) were cultured in RPMI-1640 media (Thermo Fisher Scientific, USA) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Microbiology 2023Quote: ... bacteria were grown to log phase in the presence of 1:200 pooled normal human serum (Thermo Fisher Scientific) to opsonize bacteria ...
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bioRxiv - Cell Biology 2023Quote: ... after which they were washed with 1% human albumin in PBS and stained with propidium iodide (Molecular Probes #P3566) for assessing viability ...
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bioRxiv - Cell Biology 2023Quote: ... The digested tissue was mixed with an equal volume of 1% human albumin (CSL Behring) in DPBS −/− (Gibco #14190094) to stop the lysis ...
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bioRxiv - Microbiology 2023Quote: ... Cat#40590-D001) and anti-human AF488 (1:1000 with final concentration of 2μg/ml; ThermoFisher Cat#A-11013) for secondary conjugation ...
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bioRxiv - Neuroscience 2023Quote: ... Transferrin (loading control) was detected with mouse anti-human transferrin receptor antibody (1:500, Cat# 136800, Invitrogen, Waltham, MA). Protein samples (50 µg ...
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bioRxiv - Molecular Biology 2023Quote: The gene sequences of human MCHR1(residues 70-405) and MCHR2(residues 1-331, with L2566.40Y mutation) was subcloned into pFastBac vector (Invitrogen) with an N-terminal haemagglutinin signal peptide (HA ...
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bioRxiv - Cancer Biology 2023Quote: ... murine or human (Panc-1) PDAC cells were stained in suspension with CPD670 (#65-0840-85, Thermo Fisher Scientific). After labeling ...
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bioRxiv - Immunology 2023Quote: ... purified cells were plated in 96-well round bottom plate in Yssel’s media (IMDM + 4% v/v Yssel’s supplement (made in house, Amsterdam UMC) + 1% v/v human AB serum (Invitrogen)) ...
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bioRxiv - Biochemistry 2024Quote: ... followed by staining with Alexa Fluor 647-conjugated Goat anti-Human antibody (Thermo Fisher Scientific, A-21445, 1:500).
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bioRxiv - Cell Biology 2021Quote: ... Total proteins were quantitated using Qubit Protein Assays (ThermoFisher) and equal loads of total protein were separated on a NuPAGE 4-12% Bis-Tris mini gel (NP0336BOX ...
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bioRxiv - Neuroscience 2022Quote: ... BCA protein assay kit (ThermoFisher Pierce BCA Protein Assay) was used to determine the protein concentration ...
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bioRxiv - Cancer Biology 2021Quote: ... Protein was quantified by BCA Protein Assay (Thermo Scientific) and 50 µg of total protein in 100 mM TEAB and 10% acetonitrile was incubated at 99°C for 1 h.
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bioRxiv - Genetics 2020Quote: ... protein quantification using BCA Protein Assay Kit (Thermo Fisher) was done ...
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bioRxiv - Microbiology 2020Quote: ... and proteins were immunoprecipitated with Protein A Dynabeads (ThermoFisher) conjugated to the relevant antibody (anti-MqsRA ...
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bioRxiv - Microbiology 2020Quote: ... Proteins were quantified using BSA Protein Assay Kit (ThermoFisher). Equal amounts of protein were boiled with 6X loading buffer (375mM Tris HCL ...
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bioRxiv - Microbiology 2020Quote: ... Protein concentration was measured using BCA Protein Assay (ThermoFisher). For anti-NP immunoprecipitation ...
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bioRxiv - Microbiology 2020Quote: ... Protein concentrations were determined with Bradford protein assay (Thermofisher), following the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... cas9 protein (Fisher Scientific A36498 Truecut Cas9 Protein v2) and gRNA were co-electroporated into G1 ES cells derived from an F1 hybrid blastocyst of 129S6 x C57BL/6J ...
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Allelic diversity uncovers protein domains contributing to the emergence of antimicrobial resistancebioRxiv - Microbiology 2022Quote: ... alongside protein standards (SeeBlue Pre-stained Protein Standard, Invitrogen). Gels were run at room temperature for 150 min at 80 V in running buffer ...
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bioRxiv - Plant Biology 2022Quote: ... the magnetic Protein A and Protein G Dynabeads (Invitrogen) were added and incubated at 4°C for 2 hours ...
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bioRxiv - Plant Biology 2023Quote: ... the magnetic Protein A and Protein G Dynabeads (Invitrogen) were added and mixture was rotated at 4°C for 2 hours ...
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bioRxiv - Neuroscience 2023Quote: ... cas9 protein (Fisher Scientific A36499 TRUECUT CAS9 PROTEIN V2) and the gRNA with concentrations of 10 ug ...