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Citations for Thermo Fisher :
2251 - 2300 of 10000+ citations for Human Immunodeficiency Virus Tat Protein HIV 1 Clade D UG28R since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: Human cerebral organoids are generated from human induced pluripotent stem cells (iPSCs) which were purchased from GibcoTM (Gibco by Thermofisher ...
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bioRxiv - Cancer Biology 2020Quote: ... anti-human TNF-α (hTNF-α)-PE and anti-human IFN-ɣ (hIFN-ɣ)-PECy7 were from ThermoFisher. Anti-human CD4 (hCD4)-BV711 was from Biolegend.
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bioRxiv - Immunology 2020Quote: ... using a secondary antibody recognizing all human immunoglobulin isotypes (goat anti-human IgG H+L-HRP, Invitrogen/ThermoFisher) [12,13] ...
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bioRxiv - Immunology 2021Quote: ... which contains the gene expression profile of human microglia using a human gene ST 2.0 Microarray Chip (Affymetrix). The human microglia were polarized to either pro-inflammatory (M1 ...
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bioRxiv - Immunology 2020Quote: ... using a secondary antibody recognizing all human immunoglobulin isotypes (goat anti-human IgG H+L-HRP, Invitrogen/ThermoFisher) [12,13] ...
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bioRxiv - Neuroscience 2024Quote: ... The concentrations of human α-synuclein were measured using a human α-syn-specific ELISA (ThermoFisher Scientific #KHB0061) according to the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2024Quote: ... Human full-coding KATNA1 cDNA was isolated from a human fetal brain Marathon-ready cDNA collection (Clontech, Invitrogen) with the following forward and reverse primers ...
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bioRxiv - Immunology 2023Quote: Total human IgG levels were determined following the manufacturer’s instructions in the Human IgG Total ELISA Kit (Invitrogen).
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bioRxiv - Bioengineering 2023Quote: ... primary human T cells were thawed and activated with anti-CD3/CD28 Human T-Expander Dynabeads (Thermo Fisher) at a 3:1 bead to cell ratio ...
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bioRxiv - Synthetic Biology 2023Quote: ... primary human T cells were thawed and activated with anti-CD3/CD28 Human T-Expander Dynabeads (Thermo Fisher) at a 3:1 bead to cell ratio ...
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bioRxiv - Molecular Biology 2021Quote: ... Protein A-tagged proteins were detected with HRP-conjugated polyclonal anti-Protein A (Invitrogen, PA1-26853) at 1:5000.
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bioRxiv - Developmental Biology 2022Quote: ... Proteins were resolved alongside a protein ladder (PageRuler Plus Pre-stained Protein Ladder, Thermo Fisher Scientific) on NuPAGE 4-12% BisTris gradient gels using NuPAGE MOPS SDS running buffer (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2023Quote: ... Protein concentration was quantified using Qubit™ Protein and Protein Broad Range (BR) Assay Kits (ThermoFisher).
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bioRxiv - Microbiology 2023Quote: ... and protein concentration was determined by protein assay (EZQ Protein Quantification Kit; Life Technologies, Carlsbad, CA). Ct226-FLAG was affinity purified using magnetic FLAG beads (Sigma-Aldrich ...
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bioRxiv - Microbiology 2020Quote: ... The enzymatic activity of Ddl was also assayed by measuring the release of inorganic phosphate in the reaction (similar to the D-ala-D-ala dipeptide formation assay) with EnzChek phosphate assay kit (Life Technologies). 10 μl of the reaction product was added to 790 μl of standard reaction mixture containing 230 μl dH2O ...
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bioRxiv - Plant Biology 2022Quote: ... 2006) and the cassettes cloned into pENTR/D-TOPO using a pENTR™/D-TOPO™ Cloning Kit (Thermo Fisher Scientific) and subsequently pUB-DEST (Grefen et al. ...
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bioRxiv - Cell Biology 2020Quote: ... Primary antibodies included rabbit polyclonal anti-β-COP protein (1:200; Thermo Fisher, PA1-061) and mouse monoclonal anti-flag (1:500 ...
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bioRxiv - Pathology 2019Quote: ... Proteins were stained with 1-Step™ Ultra TMB-ELISA Substrate Solution (Thermo Fisher Scientific) until blue colour was fully generated and the reaction stopped by adding 1 M H2SO4 ...
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bioRxiv - Microbiology 2019Quote: ... Labeled proteins were visualized with Alexa Fluor-conjugated secondary goat antibodies (1:2000, Life Technologies). Nuclei were visualized with the DNA stain (DAPI ...
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bioRxiv - Cell Biology 2019Quote: ... 100 μM of the protein was incubated with 1 mM tris(2-carboxyethyl)phosphine (Invitrogen) for 30 min at RT protected from light ...
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bioRxiv - Bioengineering 2021Quote: ... 1 µL of 1M DTT and 2.5 µL of 4x LDS protein loading buffer (Invitrogen) were added to the bead mixture ...
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bioRxiv - Bioengineering 2021Quote: ... STEMdiff APEL2 medium (1% (v/v) PFHM-II protein-free hybridoma medium (Thermo Fisher Scientific) and 1% (v/v ...
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bioRxiv - Biochemistry 2021Quote: ... The substrate proteins were modified with a 1:0.8 ratio Alexa Fluor 680 (Thermo Fisher) for 10 min and quenched with 5 mM DTT ...
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bioRxiv - Cancer Biology 2021Quote: ... Primary antibodies used were PON1 (18155-1-AP, Protein tech) and PON3 (OTI1A5, Thermo Scientific).
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bioRxiv - Bioengineering 2020Quote: ... rabbit anti-S100 Protein Ab-2 (S100, 1:500, Thermo Scientific Cat# RB-044-A) was used to identify Schwann cells ...
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bioRxiv - Microbiology 2021Quote: ... MeV-M proteins were probed using rabbit anti-EGFP polyclonal antibody (1:1000) (Invitrogen #A6455). The Venus fluorescent protein used in BiFC experiments is a genetic variant of EGFP ...
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bioRxiv - Cell Biology 2021Quote: ... The proteins were probed with the following antibodies: Monoclonal anti-GFP (1:2000, Thermo Scientific), anti-β-actin (1:2000 ...
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bioRxiv - Molecular Biology 2022Quote: ... the supernatant was incubated with 1/14 volume of free Protein G Dynabeads (ThermoFisher Scientific) rolling for 1 hour at 5°C to remove sticky proteins and RNAs ...
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bioRxiv - Genetics 2022Quote: ... PDE6B protein was detected by Rabbit anti-PDE6B antibody (1:500; Thermo Scientific, PA1-722). Mouse anti-GAPDH antibody (1:10000 ...
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bioRxiv - Cell Biology 2022Quote: ... at a dilution of 1:1,000 and the cytoplasmic protein GAPDH (Thermo Fisher Scientific, AM4300) at a dilution of 1:5,000) ...
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bioRxiv - Microbiology 2020Quote: ... 1 µg of purified antibodies was conjugated to 10 µl Dynabeads Protein G (Thermo Fisher) for 30 min at room temperature ...
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bioRxiv - Immunology 2020Quote: ... containing 100:1 T-PER™ Tissue Protein Extraction Reagent (Thermo Scientific™, Waltham, MA) and Halt™ Protease and Phosphatase Inhibitor Single-Use Cocktail (100X ...
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bioRxiv - Immunology 2022Quote: ... BMDMs and THP-1 cells were lysed in mammalian protein extraction reagent (MPER, Thermo Scientific) along with cOmplete Mini (Roche ...
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bioRxiv - Biochemistry 2019Quote: ... the supernatant was incubated with 25 μL (1:10 dilution) protein G beads (Invitrogen, 10003D) and 1-2 μg of antibodies for 4 h to overnight ...
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bioRxiv - Genetics 2020Quote: ... 3-Phosphoglycerate kinase (Pgk1) protein was detected with anti-Pgk1 monoclonal antibody (1:30,000; Invitrogen) and HSP90 protein was detected with a mouse anti-HSP90 monoclonal antibody (1:2000 ...
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bioRxiv - Genetics 2020Quote: ... 3-phosphoglycerate kinase (Pgk1) protein was detected using anti-Pgk1 monoclonal antibody (1:30,000; Invitrogen) as a loading control.
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bioRxiv - Cell Biology 2020Quote: Protein samples were lysed in 1% Triton X-100 (Thermo Fisher Scientific, USA, #21568-2500) with 1 mM EDTA (Promega Life Sciences ...
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bioRxiv - Biochemistry 2021Quote: ... The proteins were revealed using a V5-monoclonal antibody (V5-mAb V2660; 1:5000; Invitrogen), ACE2 antibody (rabbit monoclonal ab108252 ...
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bioRxiv - Cancer Biology 2021Quote: ... Primary antibodies recognizing the following proteins were used: Ki67 (Thermo Fisher #MA5-14520, 1:500), phospho-Rb Ser807/811 (Cell Signaling #8516 ...
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bioRxiv - Biochemistry 2022Quote: ... Proteins were digested by adding 1 μg of Pierce MS-grade trypsin (Thermo Fisher Scientific) and incubated overnight at 37°C with stirring (1,250 rpm) ...
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bioRxiv - Cancer Biology 2022Quote: ... Proteins were probed with mouse anti-Bcl-2 antibody (Thermo Fisher Scientific, MA511757, 1:100), rabbit anti-HMGB1 antibody (Cell Signaling ...
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bioRxiv - Microbiology 2022Quote: ... the eluted proteins were run 1 cm into a NuPAGE 10% Bis-Tris gel (Invitrogen) and stained with Coomassie Brilliant Blue ...
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bioRxiv - Microbiology 2022Quote: ... or 1-3 µL of PageRuler Prestained Protein Ladder (10-180 kDa) (Thermo Scientific, 26617) were used as a ladder ...
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bioRxiv - Neuroscience 2022Quote: ... and rat anti-glial fibrillary acidic protein (GFAP) Mab (ThermoFisher Scientific #13-0300, 1:500). Sections were then washed in PBST and incubated with fluorophore-conjugated secondary antibodies ...
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bioRxiv - Molecular Biology 2022Quote: ... AEBSF 1 mM) with 2 µg of antibodies and Dynabeads Protein G (Thermo Fisher Scientific). Control immunoprecipitation (IP ...
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bioRxiv - Cell Biology 2022Quote: ... 1 mM EDTA) and loaded onto magnetic beads (dynabeads, protein G, 2.8 μm diameter, Invitrogen), which were previously decorated with sub-saturating quantities of a monoclonal anti-FLAG antibody (M2 ...
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bioRxiv - Developmental Biology 2023Quote: ... supplemented with 1% (v/v) PFHM-II Protein-Free Hybridoma Medium (Thermo Fisher Scientific, # 11370882), hereafter referred to as APEL™2 ...
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bioRxiv - Molecular Biology 2023Quote: ... 1 mg lysate was pre-cleared with Dynabeads™ protein G (cat# 10004D, Thermo Fisher) for 1 h with rotation at 4°C ...
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bioRxiv - Biochemistry 2023Quote: ... then 1 hour with Pierce™ Protein A/G agarose beads (Thermo Fisher Scientific, 20421). Beads were washed 4 times with 0.1% NP-40 substitute (VWR ...
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bioRxiv - Neuroscience 2023Quote: ... the remaining lysate precleared for 1 hour with Protein-A Dynabeads (Thermo Scientific, no. 10001D). FLAG-M2 antibody Protein-A Dynabead conjugate was incubated with the precleared lysate overnight ...