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Citations for Thermo Fisher :
1901 - 1950 of 10000+ citations for Human Immunodeficiency Virus Tat Protein HIV 1 Clade D UG28R since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... 5% CO2 in minimal medium containing 1 part DMEM (4.5 g/L D-Glucose, Pyruvate, L-Glutamine; Invitrogen 11971-025): 1 part DMEM/F12 ...
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bioRxiv - Neuroscience 2022Quote: ... Cardiac perfusion began with 25mL of chilled 1×PBS (pH 7.4) followed by perfusion of 10mL DiI solution (Sigma Aldrich, D-282, #42364, Invitrogen), and finally perfusion with 25mL of chilled fixative 4% paraformaldehyde ...
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bioRxiv - Immunology 2020Quote: ... M-014142-00-0005) or non-targeting siRNA Pool #1 (Dharmacon, Horizon Discovery, D-001206-13-05) were transfected using Lipofectamine RNAiMax (Invitrogen), and experiments were performed at 48 hours post-transfection.
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bioRxiv - Cancer Biology 2021Quote: ... carrying the mutation R273C was first amplified by PCR using the primers hp53-1 (5’-CACCATGGAGGAGCCGCAGTCAGATCC-3’) and hp53-8 (5’-GGATCCTCAGTCTGAGTCAGGCCCTTCTGTCTTG-3’) and cloned into the pENTR/D-TOPO vector (ThermoFisher) generating the entry vector pENTR p53(R273C ...
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bioRxiv - Cell Biology 2019Quote: ... 2 mM D-Glucose, 2 mM L-Glutamine, 1 mM Sodium Pyruvate, 140 mM KCl and 20 mM HEPES, Invitrogen) for longer imaging sessions ...
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bioRxiv - Neuroscience 2020Quote: ... Prior to transfection cells were brought into suspension by trypsinization and resuspension to 0.18 million cells/mL in growth medium (D-MEM, Gibco 10566016; supplemented with 10% Fetal Bovine Serum, Gibco 10270106; 1% Sodium Pyruvate, Gibco 11360039 ...
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bioRxiv - Developmental Biology 2020Quote: ... Sections were then blocked with 5% normal donkey serum in 1X PBS for one hour before overnight incubation in primary antibodies (Foxf1, goat, R&D, 1:300; Sox9, mouse, Invitrogen, 1:200 ...
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bioRxiv - Cancer Biology 2020Quote: ... followed by DAPI staining at a final concentration of 0.5 μg/ml in 1x PBS (1:5000, #D-1306, Life Technologies) and 2 additional washes with PBS ...
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bioRxiv - Developmental Biology 2019Quote: ... sections were washed in PBS before blocking in PBS-0.1% Triton-10% normal donkey serum (NDS) and overnight incubation with primary antibody (1:50, anti-HuC/D, Molecular Probes) in PBS-0.1% Triton-1% NDS ...
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bioRxiv - Cell Biology 2021Quote: ... 293-EBNA-1 human embryonic kidney cells expressing full-length human VEGF-D (“293-EBNA-1-VEGF-D cells”) were established previously (36) and cultured in Dulbecco’s modified Eagle’s medium (DMEM, Thermo Fisher Scientific), 10% FBS ...
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bioRxiv - Immunology 2022Quote: ... cut into small pieces and digested with collagenase D and DNase I for 1 h at 37 °C in HBSS (Gibco). After digestion ...
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bioRxiv - Neuroscience 2022Quote: ... 5 mM EDTA and 1% BSA in HBSS) and 2μL of 7-aminoactinomycin D (7-AAD, Thermo Fisher Scientific, A1310) at a concentration of 0.1 mg/mL were added ...
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bioRxiv - Genomics 2023Quote: ... Each individual tissue was transferred to a Lysing Matrix D tube (MP, 6913500) with 1 mL of TRIzol (Ambion, 15596018) and homogenized using a Beadbug 6 microtube homogenizer (Benchmark Scientific ...
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bioRxiv - Developmental Biology 2023Quote: ... 2017) using the oligonucleotides kniRR Fw and kniRA Rv (Supple. Table 1) and cloned in p-ENTR/D-TOPO (Invitrogen). Cloning was confirmed by PCR using the oligonucleotides M13 Fw and kniRA Rv (Supple ...
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bioRxiv - Immunology 2023Quote: ... supplemented with 10% Fetal Bovine Serum (FBS) from R&D Biosystems (Cat. No. S11150, Minneapolis, MN, USA) and 1% Penicillin/Streptomycin from Gibco/Thermo Fisher Scientific (Cat ...
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bioRxiv - Microbiology 2023Quote: ... 1% n-Dodecyl β-D-maltoside (DDM) and 0.05% Triton X-100) supplemented with 1 X HALT protease and phosphatase inhibitor cocktail (Thermo Fisher). The lysate was centrifuged at 14,000 rpm for 20 min at 4°C and supernatant was transferred to new tubes and normalized for total protein amounts determined by BCA assay ...
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bioRxiv - Neuroscience 2023Quote: ... their columns were excised in a dissection medium constituted of a solution of D-glucose 6.5 mg ml-1 in DPBS (Gibco; Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... coated #1.5 18 mm glass coverslips in DMEM (4.5 g/L-1 D-G-glucose) supplemented with 10% fetal bovine solution (Gibco™), 1% antibiotic solution (penicillin Streptomycin ...
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bioRxiv - Neuroscience 2024Quote: ... supplemented with 15% fetal bovine serum (FBS; G22133, R&D) supplemented with 1× penicillin-streptomycin (Cat.# 15140-122; Thermo Scientific) at 37 °C ...
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bioRxiv - Cell Biology 2024Quote: ... Cultures were then allowed to egress for 30 min at 37 °C in media supplemented with 1 μM cytochalasin D (Invitrogen). After incubation ...
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bioRxiv - Cell Biology 2022Quote: ... Control (vehicle: 40 μM HCL, 0.002% BSA [0-5 d]; 0.1% DMSO [5-10 d]; all from Thermo Fisher Scientific) for 0-10 d [=Baseline] ...
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bioRxiv - Plant Biology 2022Quote: ... The PRP39a genomic fragment was amplified from the first ATG to the penultimate codon to omit the stop codon and cloned into the pENTR/D TOPO vector using pENTR/D TOPO cloning kit (Invitrogen). PRP39a was then transferred into the plant expression vector pB7FWG2 (Karimi et al. ...
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bioRxiv - Bioengineering 2023Quote: ... iPSCs were then differentiated into definitive endoderm by treatment with 100 ng/mL Activin A (R&D) and 15 ng/mL BMP4 (R&D) in RPMI supplemented with nonessential amino acids (NEAA, Gibco) for 1 day ...
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bioRxiv - Microbiology 2020Quote: ... the sections were sequentially incubated with mouse polyclonal antibody to SARS-CoV-2 N protein (1:500 dilution) and HRP-conjugated goat anti-mouse IgG secondary antibody (1:5000 dilution, Invitrogen). The sections were observed under microscope (Olympus ...
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Inositol depletion regulates phospholipid metabolism and activates stress signaling in HEK293T cellsbioRxiv - Cell Biology 2022Quote: ... Protein detection was done using a custom antibody against MIPS (1:10,000) and anti-actin (1:10,000; Invitrogen MA1-744). The custom MIPS antibodies were produced by YenZym Antibodies ...
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bioRxiv - Cell Biology 2022Quote: ... The surviving clones were screened for expression of the desired proteins in the presence of 1 μg ml−1 doxycycline (Invitrogen). Expression of Myc-Cdk11 was also induced with 1 μg ml−1 doxycycline in the subsequent experiments.
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bioRxiv - Microbiology 2021Quote: ... the sections were incubated with house-made mouse anti-SARS-CoV-2 nucleocapsid protein serum (1:5000) and HRP465 conjugated goat anti-mouse IgG secondary antibody (1:5000 dilution, Invitrogen). The lung sections from the vector-transduced mouse were used as negative control ...
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bioRxiv - Bioengineering 2021Quote: ... Immunostaining of BBB constructs for tight junction protein ZO-1 was conducted using ZO-1 mouse monoclonal antibody conjugated with Alexa 488 (ThermoFisher). Hydrogels were removed from the transwell insert using a biopsy punch ...
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bioRxiv - Biochemistry 2020Quote: ... 1 mM TCEP and SYPRO orange protein gel stain at a 1:500 dilution of the manufacturer’s stock (Invitrogen, S6651). The samples also contained 5 μM NBD protein ...
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bioRxiv - Pathology 2020Quote: ... 1 mM EDTA and 1% SDS) and the protein concentration was quantified by the BCA assay (Thermo Fisher Scientific, #23225). Equal amount of whole cell extracts were resolved by SDS-PAGE and transferred to PVDF membranes (Millipore ...
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bioRxiv - Microbiology 2021Quote: ... Mice received 20 μg protein antigen (vaccinated) or PBS (mock-vaccinated) mixed 1:1 with Imject Alum adjuvant (Thermofisher Scientific) in a total volume of 100 μl ...
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bioRxiv - Microbiology 2023Quote: ... were coated with 100 µL of recombinant SARS-CoV-2 S protein (Wuhan-1 strain and BA.5) at a concentration of 1 µg/mL in PBS (Gibco) at 4°C overnight ...
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bioRxiv - Molecular Biology 2023Quote: ... chromatin-antibodies complexes were recovered by incubation with 30 μl of Dynabeads® Protein A/G 1:1 mix (ThermoFisher) for 2 h at 4°C under rotation ...
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bioRxiv - Genomics 2023Quote: ... Sonicated samples were pre-cleared to remove background signal through incubation with a 1:1 mix of Protein A/G Dynabeads (InVitrogen). Antibody was added at a concentration of 1 µg/µL per sample and immunoprecipitated at 4°C overnight ...
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bioRxiv - Immunology 2024Quote: ... Any bound proteins were eluted by adding a 1:1 mix of 10 mM TBS and LDS buffer (Life Technologies) to the first well and then transferring from well to well ...
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bioRxiv - Neuroscience 2020Quote: Total protein concentrations of isolated protein lysates were quantified by BCA protein assay kit (ThermoFisher) and equal protein amounts were boiled at 95°C for 10 minutes ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Total protein concentration determined by BCA protein assay (Pierce BCA Protein Assay Kit, Thermo Scientific). Filter-aided sample preparation (FASP ...
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bioRxiv - Immunology 2021Quote: ... a 1 mg/mL solution of these proteins was mixed with the Protein Thermal Shift™ Dye kit (Thermo Fisher, Waltham, MA, USA; Cat # 4461146) in triplicate as per the manufacturer’s instructions ...
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A lateral protrusion latticework connects neuroepithelial cells and is regulated during neurogenesisbioRxiv - Developmental Biology 2021Quote: ... The pENTR/D-TOPO cloning kit (ThermoFisher, K240020) was used to introduce the PCR products into the entry vector for Gateway cloning ...
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bioRxiv - Molecular Biology 2021Quote: ... 5 mMand 33 mM D-glucose (Gibco, USA) were added to the medium for 48 h ...
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bioRxiv - Neuroscience 2019Quote: ... and complement factor D (ThermoFisher-Invitrogen, LaFayette, CO). The mean value for all determinations of CD81 in each assay group was set at 1.00 ...
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bioRxiv - Neuroscience 2019Quote: ... and complement factor D (ThermoFisher-Invitrogen, LaFayette, CO). The mean value for all determinations of CD81 in each assay group was set at 1.00 ...
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bioRxiv - Plant Biology 2019Quote: ... cloned into pENTR/D-TOPO (Thermo Fisher Scientific) and subsequently transferred into the GATEWAY-compatible destination vector pH7FWG2.0 (Karimi et al. ...
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bioRxiv - Immunology 2021Quote: ... DMEM with 4.5 g/L D-glucose (Gibco) containing 1:100 SM1 (StemCell Technologies) ...
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bioRxiv - Cancer Biology 2020Quote: ... an Affymetrix Clariom™D (Thermo Fisher, 902922) was applied and an Affymetrix GeneChip™ miRNA 4.1 platform was used for miRNA analysis (Thermo Fisher ...
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bioRxiv - Plant Biology 2021Quote: ROMT2-3 were amplified using TOPO-D (Invitrogen) compatible primers from cDNA derived from grapevine senescent leaves ...
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bioRxiv - Plant Biology 2020Quote: ... and were cloned into pENTR/D-TOPO (Invitrogen) (Figs ...
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bioRxiv - Plant Biology 2020Quote: ... inserted into pENTR/D-topo (Thermo Fisher Scientific) and recombined into the pGREEN800LUC plasmid (Hellens et al. ...
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bioRxiv - Genomics 2021Quote: ... using oligo-d(T)(x20)-alexa488 (Invitrogen 7206906) DNA probe ...
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bioRxiv - Plant Biology 2021Quote: ... was cloned into a pENTR/D-TOPO (Invitrogen) vector ...