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Citations for Thermo Fisher :
2101 - 2150 of 10000+ citations for 5 M TOLYL FURAN 2 CARBALDEHYDE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: Cellular glucose uptake was measured by culturing cells in glucose free DMEM for 2 hours before adding 100µM of the fluorescent glucose analogue 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl) Amino)-2-Deoxyglucose (2-NBDG; ThermoFisher, N13195). The analogue was incubated with MCF7s for 30 minutes and MSCs for 60 minutes at 37°C ...
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bioRxiv - Biochemistry 2022Quote: ... 2 mM Tris(2-carboxyethyl)phosphine (TCEP, ThermoFisher)) supplemented with a protease inhibitor tablet (Roche ...
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bioRxiv - Genetics 2019Quote: ... 2 μl of probe (2 μM; Applied Biosystems) and 2 μl of template DNA (where extracts were normalised to 34 ng/μl) ...
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bioRxiv - Molecular Biology 2020Quote: ... 2 (Thermofisher). A reverse transfection approach was used to deplete AurkA and CEP135 ...
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bioRxiv - Systems Biology 2023Quote: ... 2% (ThermoFisher). Purified amplicons were quantified by Qubit™ dsDNA Quantification Assay Kits (ThermoFisher ...
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bioRxiv - Immunology 2021Quote: ... were co-transfected with the EGFP-Caspase 2 or EGFP-Δ Caspase 2 plasmid and the synthesized miR-2 (miR-2-mimic) using Cellfectin II transfection reagent (Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2020Quote: Macrophages were incubated with the fluorescent glucose analog 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (2-NBDG) (10 μM, Invitrogen, California, USA) in PBS for 30 min ...
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bioRxiv - Immunology 2021Quote: ... containing 2’-(N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)-2-deoxyglucose (2-NBDG; 50 μM; Thermo Fisher Scientific). To determine FA uptake ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... 2-(N-[7-nitrobenz-2-oxa-1, 3-diazol-4-yl] amino)-2-deoxyglucose (2-NBDG) (Molecular Probes-Invitrogen, CA, USA) was used to assess glucose uptake in L6 cells ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... 2-(N-[7-nitrobenz-2-oxa-1, 3-diazol-4-yl] amino)-2-deoxyglucose (2-NBDG) (Molecular Probes-Invitrogen, CA, USA) was used to assess glucose uptake in L6 cells ...
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bioRxiv - Cell Biology 2024Quote: ... Glucose was traced using 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (2-NBDG, Invitrogen, ThermoFisher, cat. #N13195) combined with 0,57 mg/mL 40kDa tetramethyl-rhodamine Dextran (Invitrogen ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... 2-(N-[7-nitrobenz-2-oxa-1, 3-diazol-4-yl] amino)-2-deoxyglucose (2-NBDG) (Molecular Probes-Invitrogen, CA, USA) was used to assess glucose uptake in Caco2 cells ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... 2-(N-[7-nitrobenz-2-oxa-1, 3-diazol-4-yl] amino)-2-deoxyglucose (2-NBDG) (Molecular Probes-Invitrogen, CA, USA) was used to assess glucose uptake in Caco2 cells ...
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bioRxiv - Immunology 2023Quote: ... stained with Ghost-510 and 2-(N-Nitrobenz-2-oxa-1,3-diazol-4-yl)amino)-2-deoxyglucose (2-NBDG; 60 µM) (Thermo Fisher Scientific) (30 min at 37 C) ...
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bioRxiv - Biochemistry 2022Quote: ... and 5 µg/mL and 5 units/mL penicillin-streptomycin (Gibco). One T175 flask of HEK293 cells of 70% confluency per condition was transiently transfected using 90 µg of EGFP-PABPi or EGFP-PABPi mut ...
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bioRxiv - Neuroscience 2021Quote: ... 5 U/ml penicillin and 5 U/ml streptomycin (Invitrogen, USA). Cells were plated on poly-D-lysine (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2021Quote: ... using forward primer 5’-CACAGGAAGCCCTGGAAGCT and reverse primer 5’-GAGCAGGTCAGGTTTTTGGA (Invitrogen). SIGLEC22P was amplified similarly with forward primer 5’-GCACCTCAGAGTGGAAGGAC and reverse primer 5’-GAAGGGGTGACTGAGGTACA ...
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bioRxiv - Cell Biology 2021Quote: ... with forward primer 5’-GCACTTGCAGCCGGATTTTTGGATCCATAGCCAGGGCC and reverse primer 5’-GGCCCTGGCTATGGATCCAAAAATCCGGCTGCAAGTGC (Invitrogen) to generate the pcDNA3.1-KIR-CD33-V5/HIS vector ...
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bioRxiv - Biochemistry 2024Quote: hSSB2 was labeled with 5-IAF (5-iodoacetamido-fluorescein, Thermo Fisher) on the intrinsic cysteines ...
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bioRxiv - Cell Biology 2023Quote: ... and 5 µL of 5% ammonium persulfate (APS) (Thermo Scientific, 17874). 50 µl was added to a strip of parafilm in a humidity chamber on ice ...
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bioRxiv - Biophysics 2023Quote: ... samples were incubated with a 5 μM DRAQ-5 (Thermo Scientific) DNA staining buffer at 37° C for 30 minutes with agitation ...
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bioRxiv - Biochemistry 2024Quote: ... 0.3 × 5 mm trapping column (5 µm, 100 Å, Thermo Scientific) and analyzed by nanoLC-ESI-MS/MS analysis using a Thermo Ultimate 3000 RSLC nanoUPLC coupled online to an Orbitrap Exploris™ 240 mass spectrometer (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2024Quote: ... containing 5 unit/mL penicillin and 5 ug/mL streptomycin (Gibco).
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bioRxiv - Genetics 2021Quote: ... The expression levels of candidate genes were then quantified through qPCR using the 2 × T5 Fast qPCR Mix (TsingKe, Beijing, China) on an ABI QuantStudio 5 system (Thermo Fisher, Waltham, MA, USA). The GAPDH gene was used as the internal reference and the final volume of the qPCR reaction was 20 μl ...
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bioRxiv - Cancer Biology 2021Quote: Cell proliferation was examined by 5-bromo-2-deoxyuridine (BrdU) cell proliferation enzyme-linked immunosorbent assay (ELISA) according to the kit manufacturer’s protocol (Life Technologies, Grand Island, NY, USA). The cells were incubated in presence of various concentrations of CLEFMA (0 – 10 µM) ...
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bioRxiv - Neuroscience 2019Quote: ... Peptide mixtures were loaded to a μ-precolumn Acclaim C18 PepMap100 (100 μm × 2 cm, 5 μm, 100 Å, C18 Trap column; Thermo Fisher Sci.) at a flow rate of 15 μL/min and separated using a C18 Acclaim PepMap RSLC analytical column (75 μm × 50 cm ...
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bioRxiv - Neuroscience 2020Quote: ... HEPES media for 30 minutes at 37°C with 5% CO2 and 2 drops/mL of NucBlue™ Live ReadyProbes™ Reagent (Invitrogen™, R37605) for 20 minutes ...
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bioRxiv - Biochemistry 2020Quote: The MuSIC-probe labeled antibodies from above are suspended in 200 ml of 0.02% (2 ml/10ml) Tween 20 (Fisher Scientific Cat: 9005-64-5) in PBS and added to 50 ml of protein A dynabeads (Fisher Scientific Cat ...
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bioRxiv - Pathology 2020Quote: ... Acclaim PepMap, 100 μm × 20 mm, 5 μm, 100 Å, separation column: Acclaim PepMap, 75 μm × 500 mm, 2 μm, 100 Å, Thermo Fisher Scientific) equipped with the Dionex UltiMate 3000 RSLCnano System (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... and SARS-CoV-2 RdRp IP2-targeted RdRp Institute Pasteur RT-PCR was performed on a QuantStudio™ 5 System (Applied Biosystems, Thermo Fisher Scientific). Log10 reduction values (LRV ...
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bioRxiv - Biochemistry 2020Quote: ... The nanoLC system was equipped with an Acclaim™ Pepmap™ 100 C18 nano-trap column (1×2 cm, 5 μm particle size, Thermo Scientific, USA) and an Acclaim™ Pepmap™ 100 C18 analytical column (length 15 cm ...
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bioRxiv - Neuroscience 2022Quote: ... slides were rinsed with 0.025% Triton X-100/1xTBS 2 × 5 min and incubated in goat anti-rabbit AlexaFlour488 secondary antibody (1:1000, Life Technologies, #A27034, Carlsbad, CA) at RT for at least 2 h followed by 4’,6-diamidino-2-phenylindol (DAPI ...
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bioRxiv - Immunology 2022Quote: We estimated hemocyte differentiation by visualizing and quantifying the synthesis of new DNA in vivo based on 5-ethynyl-2′-deoxyuridine (EdU) incorporation and subsequent detection using the Click-iT EdU Alexa Fluor 647 kit (Invitrogen, Grand Island, NY, USA) as previously described (36,37) ...
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bioRxiv - Plant Biology 2022Quote: ... The seedlings were screened by microscopy for YFP and then fixed and processed for immunofluorescence microscopy using a 1:2000 dilution of monoclonal anti-α-tubulin B-5-1-2 antibody (Life Technologies; 32-2500) followed by 1:2000 dilution of Alexa-568 goat anti-mouse antibody (Thermo Fisher ...
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bioRxiv - Plant Biology 2023Quote: ... Peptides were reconstituted in 0.1% trifluoroacetic acid (TFA) and 2% acetonitrile (ACN) and loaded onto a PepMap C18 5 µm 1 cm trapping cartridge (Thermo-Fisher Scientific, Waltham, MA, USA) at 12 µL/min for 6 min before switching the pre-column in line with the analytical column (nanoEase M/Z Peptide BEH C18 Column ...
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bioRxiv - Molecular Biology 2022Quote: ... Cell proliferation was also assessed by 5-ethynyl-2’-deoxyuridine (EdU) incorporation assay using Click-iT™ EdU Alexa Fluor™ 647 Flow Cytometry Assay Kit (Thermo Fisher Scientific) according to the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2024Quote: ... The gel was prepared by adding 5 µL of the PCR probes and 2 µL of the ladder (Thermo Fisher Scientific, Waltham, MA, USA) and running the reaction at 90 V for 2 hours and 45 minutes ...
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bioRxiv - Neuroscience 2024Quote: Cultured sensory neurons from wild-type and TRESK-/- mice were loaded with 5 μM fura-2/AM (F1221, Invitrogen, ThermoFisher Scientific, Waltham, MA, USA) for 45-60 min at 37°C in culture medium ...
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bioRxiv - Microbiology 2023Quote: ... Bodies and legs/wings were placed into 2-mL tubes containing 500 µL PBS and a 5-mm glass bead (Thermo Fisher Scientific, Emeryville, CA), then homogenized at 30 hertz for 5 minutes in a TissueLyser (Retsch ...
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bioRxiv - Molecular Biology 2022Quote: ... and U-2 OS cells were cultured at 37°C in 5% CO2 and maintained in DMEM supplemented with 10% FBS (Thermo Fisher Scientific, Waltham, MA). Cells were routinely tested (negative ...
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bioRxiv - Microbiology 2023Quote: ... mock and SARS-CoV-2 infected cultures were washed with calcium/magnesium-free PBS and disassociated using TrypLE (Gibco, 5 minutes at 37° C), after which samples were centrifuged (200 x g ...
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bioRxiv - Neuroscience 2024Quote: ... we washed the sections six times (5 min each) and then incubated them for 2 hours in donkey anti-rat-Alexa Flour 594 antiserum (Invitrogen, Product#: A21209, Lot#: 1744721) and biotinylated donkey anti-rabbit secondary antiserum (Jackson ImmunoResearch ...
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bioRxiv - Cancer Biology 2024Quote: ... Samples were trapped on a C18 guard- desalting column (Acclaim PepMap 100, 75μm x 2 cm, nanoViper, C18, 5 µm, 100Å, Thermo Fisher Scientific, Waltham, MA, USA), and separated on a 50 cm long C18 column (Easy spray PepMap RSLC ...
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bioRxiv - Cancer Biology 2021Quote: ... Reverse transcription was conducted with M-MLV reverse transcriptase (Thermo Fisher Scientific, Cat # 28025) and random primers (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2021Quote: ... and resuspended in mammalian protein extraction reagent (M-PER; Thermo Scientific, Rockford, IL, USA)17 ...
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bioRxiv - Cell Biology 2020Quote: ... and retro-transcribed by using 100 units of M-MLV Reverse transcriptase (Life Technologies) in the presence of 1.6 µM oligo(dT ...
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bioRxiv - Cell Biology 2020Quote: Whole-cell lysates were prepared in M-PER mammalian protein extraction reagent (ThermoFisher Scientific) containing a Halt™ protease inhibitor cocktail (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... These Peptides (35 µM) were incubated with Dynabeads™ M-280 streptavidin (Invitrogen # 11205D) in binding buffer (20 mM HEPES pH 7.5 ...
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bioRxiv - Developmental Biology 2020Quote: ... 1/10th volumes of 3 M Sodium Acetate and 30 μg Glycoblue coprecipitant (Ambion).
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bioRxiv - Molecular Biology 2020Quote: ... 30 μL 0.1 M DTT (SuperScript™ III Reverse Transcriptase Kit, Invitrogen Cat #18080044), 45 μL 1 M Tris-HCl (pH 8.0 ...