Labshake search
Citations for Thermo Fisher :
2001 - 2050 of 10000+ citations for 5 M TOLYL FURAN 2 CARBALDEHYDE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Bioengineering 2023Quote: ... and subsequently bound to Dynabeads M-280 Streptavidin (Invitrogen Thermo Fisher Scientific, USA) at room temperature for 30 minutes.
-
bioRxiv - Molecular Biology 2023Quote: ... Biotinylated proteins were enriched using magnetic streptavidin beads (Dynabeads M-280, Invitrogen, USA). The beads were washed with a series of SDS buffer ...
-
bioRxiv - Bioengineering 2023Quote: ... 1 µg RNA was reverse-transcribed using M-MLV Reverse Transcriptase Kit (Invitrogen). qRT-PCR was performed using SYBR Green (Roche ...
-
bioRxiv - Molecular Biology 2023Quote: ... The supernatants were incubated with 30 μl of Dynabeads M-270 Streptavidin (Invitrogen), preequilibrated with lysis buffer containing 1% Triton X-100 ...
-
bioRxiv - Bioengineering 2023Quote: ... and subsequently bound to Dynabeads M-280 Streptavidin (Invitrogen Thermo Fisher Scientific, USA) at room temperature for 30 minutes.
-
bioRxiv - Microbiology 2023Quote: ... 0.8 µl DTT (0.5 M) 40 U SUPERase-InTM RNase Inhibitor (Ambion®) and 43.7 µl 2% Sarkosyl for run-ons with sarkosyl ...
-
bioRxiv - Cancer Biology 2023Quote: ... 1000 ng total RNA is reverse transcribed using M-MLV Reverse Transcriptase (Invitrogen) cDNA synthesis kit following the manufacturer’s protocol ...
-
bioRxiv - Cancer Biology 2024Quote: ... cDNA was made using the M-MLV reverse transcriptase (Invitrogen, catalog number 28025013). qPCR was performed using Power up SYBR Green Master Mix (Thermo Fisher ...
-
bioRxiv - Physiology 2024Quote: ... and cells were lysed in mammalian protein extraction reagent (M-PER; Thermo Fisher) containing protease inhibitors cocktail (Roche) ...
-
bioRxiv - Bioengineering 2024Quote: ... and brought to a pH of 6.5 using 1 M NaOH (Fisher Scientific). To assemble the functionalized alginate gels ...
-
bioRxiv - Neuroscience 2024Quote: ... brains were removed and frozen in embedding medium (M-1 Embedding Matrix, ThermoFisher) before storage at −80°C for at least 12h ...
-
bioRxiv - Biochemistry 2024Quote: ... Protein was isolated using M-PER Mammalian Protein Extraction Reagent (Thermo Fisher Scientific), and Halt Protease and Phosphatase Inhibitor Cocktail (Thermo Fisher Scientific) ...
-
bioRxiv - Cell Biology 2024Quote: ... a 0.5 M solution (filter sterilized) of N-acetyl Cysteine (NAC) (Thermofisher, A15409.14) was used to add NAC at 5 mM to HEPES-NaOH (5 mM ...
-
bioRxiv - Microbiology 2024Quote: ... a 100 µL of stop solution (0.16 M H2SO4, Thermo Fisher Scientific # N600) was added and the absorbance was recorded at 450 nm using a Biotek H1M monochromatic plate reader ...
-
bioRxiv - Cancer Biology 2020Quote: ... and ITS (5 μg/mL insulin, 5 μg/mL transferrin and 5 ng/ml sodium selenite, Invitrogen). SK-N-BE (2 ...
-
bioRxiv - Immunology 2021Quote: ... cells were incubated in 2-(N-(7-nitrobenz-2-oxa-1,3-diaxol-4-yl) amino)-2-deoxyglucose (2-NBDG) (Invitrogen) (20 µM ...
-
bioRxiv - Physiology 2021Quote: ... 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (2-NBDG, Thermo Fisher Scientific, USA) was dissolved in saline solution at 5 mg/ml ...
-
bioRxiv - Cancer Biology 2020Quote: ... (2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (2-NBDG) (100 μM; ThermoFisher Scientific) with Hoechst (1 μg/mL ...
-
bioRxiv - Physiology 2019Quote: ... 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (2-NBDG, Thermo Fisher Scientific, USA) was dissolved in PBS at 5 mg/ml ...
-
bioRxiv - Immunology 2020Quote: ... Glucose uptake assay was performed by incubating cells with 50 µM 2-NBDG (2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose) (Invitrogen) for 90 min at 37°C prior to cell staining ...
-
bioRxiv - Immunology 2022Quote: ... cells were resuspended in glucose-free media containing 150 μM 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (2-NBDG Thermofisher) and incubated for 45 minutes at 37°C.
-
bioRxiv - Cancer Biology 2021Quote: ... 5% CO2 incubator with 5 μM MitoSox Red (Thermo Fisher) in MAS (70mM sucrose ...
-
bioRxiv - Cell Biology 2020Quote: ... EndoB1 siRNA 5’-UGUUUAUACGACUUGGAGCUU-3’ and 3’-AAGCUCCAAGUCGUAUAAACA-5’ (Invitrogen), control siRNA (Ambion) ...
-
bioRxiv - Genetics 2020Quote: ... each well contained: 5 μL 5× Phusion HF buffer (ThermoFisher), 17.25 μL dH2O ...
-
bioRxiv - Microbiology 2021Quote: ... 5% A+ human serum and 5% AlbuMAX II (Life Technologies). Gametocyte media was changed daily without the addition of fresh erythrocytes for 14 days following induction ...
-
bioRxiv - Neuroscience 2022Quote: ... 5% Pen-Strep and 5% glutamine (Thermo Fisher Scientific #25030081) 1-2 hours before transfection ...
-
bioRxiv - Developmental Biology 2022Quote: ... or 0.2 mM 5-ethynyl uridine (5-EU) (Thermo Fisher) in media for 30 minutes according to kit instructions ...
-
bioRxiv - Microbiology 2023Quote: ... and m7 G(5′)ppp(5′)G Cap Analog (Ambion). BHK-21 cells in 6-well plates were transfected with the capped-SINV-AaBec243-260-mCh-Flag or capped-SINV-mCh-Flag mRNA using Lipofectamine LTX (Invitrogen ...
-
bioRxiv - Cell Biology 2023Quote: ... siPALS1 (5’-UUCCUUAUGAUGAACUGGCtt-3’) and siPATJ (5’-CCAGAUACUCACACUUCAGtt-3’, Ambion), siARP2/3 (5’- GGAUUCCAUUGUGCAUCAAtt-3’ ...
-
bioRxiv - Systems Biology 2020Quote: ... fixed and blocked HeLa CCL-2 cells were incubated with 5 μg/mL Cy5 labeled rabbit anti-Ki67 primary antibodies (Thermo Fisher Scientific; RB1510P1ABX) in the 1X blocking buffer for 45 min at room temperature ...
-
bioRxiv - Cell Biology 2020Quote: ... or BODIPY™ 558/568 C12 (4,4-Difluoro-5-(2-Thienyl)-4-Bora-3a,4a-Diaza-s-Indacene-3-Dodecanoic Acid) (Thermo Fisher Scientific, Waltham, MA).
-
bioRxiv - Developmental Biology 2020Quote: ... FseI restriction sites introduction by Phusion Green High-Fidelity DNA Polymerase PCR, using manufacturers protocol (30cycles, 2-step protocol, no GC additive, 5% DMSO) (Thermo Fisher Scientific, F534S), and JM120F/R ...
-
bioRxiv - Developmental Biology 2021Quote: A dam at E16.5 in gestation was given an intraperitoneal (IP) injection with EdU (5-ethynyl-2’-deoxyuridine) from the Click-iTTM EdU Alexa FluorTM 647 Imaging Kit (Invitrogen, Carlsbad, CA, USA) at a dosage of 0.25mg /10g body weight ...
-
bioRxiv - Developmental Biology 2021Quote: Embryos and larvae from 9 hpf to 4 wpf were incubated in 3 µM 5-Ethynyl-2′-deoxyuridine (EdU; ThermoFisher Scientific, cat#: C10639) in FSW for 15-30 min ...
-
bioRxiv - Immunology 2022Quote: ... 20 minutes in 30 mL mucus dissociation solution (5 mM dithiothretol [DTT; Invitrogen 15508] and 2% heat-inactivated fetal calf serum [FCS; Gibco A38401] in HBSS); 25 minutes in 30 mL epithelial removal solution (5 mM EDTA and 2% FCS in HBSS) ...
-
bioRxiv - Immunology 2019Quote: ... Peptides dissolved in 0.1% TFA were pre–concentrated on a C18 trap column (Acclaim PepMap 100; 100 μm × 2 cm, 5 μm, 100 Å; Thermo Fisher Scientific) within 7 minutes at a flow rate of 30 μl/min with 0.1% TFA ...
-
bioRxiv - Systems Biology 2019Quote: The 72 fractions obtained from the OG fractionation (3 TMT x 24 fractions) were loaded on a trap nanocolumn (0.01 x 2 cm, 5 μm; Thermo Fisher Scientific, Massachusetts, USA) and separated with a C-18 reversed-phase (RP ...
-
bioRxiv - Bioengineering 2020Quote: ... chips were washed with 100 ul PBS per channel and stained with the following secondary antibodies diluted 1: 100 in 5% BSA in PBS for 2 hours at room temperature: goat anti-rat Alexa Fluor 488 (Thermo Fisher Scientific A11006), donkey anti-rabbit Alexa Fluor 647 (Thermo Fisher Scientific A31573) ...
-
bioRxiv - Cell Biology 2021Quote: ... samples were washed with 2% BSA in PBS for 5 min and mounted using ProLong™ Gold Antifade Mountant with DAPI (ThermoFisher Sci., USA). To preserve islets structural integrity ...
-
bioRxiv - Microbiology 2020Quote: ... Samples were concentrated onto the trap column at 5 μl min-1 for 5 min with Buffer A (0.1% formic acid, 2% Acetonitrile) and infused into an LTQ-Orbitrap Elite (Thermo Scientific, San Jose CA) at 300 nl min-1 via the analytical column using a Dionex Ultimate 3000UPLC (Thermo Scientific) ...
-
bioRxiv - Cell Biology 2021Quote: ... Cultures were maintained at 37°C in 5% CO2 for 2 h before the plating medium was replaced with Neurobasal growth medium containing B27 supplements (Life Technologies Inc., USA). The cells were used for experiments after 6 days in culture ...
-
bioRxiv - Cancer Biology 2021Quote: ... SK-MEL-2 ATCC® HTB-68™) and cultured in DMEM (Mediatech, Manassas, VA, USA) with 5% FBS (Invitrogen, Carlsbad, CA, USA) and 1% Penicillin-Streptomycin (Thermo Fisher Scientific ...
-
bioRxiv - Cell Biology 2021Quote: ... After pre-concentration and washing of the sample on a Acclaim PepMap 100 C18 column (2 cm × 100 μm i.d., 100 Å pore size, 5 μm particle size, Thermo Scientific 164564-CMD), peptides were separated on a LC EASY-Spray C18 column (50 cm × 75 μm i.d. ...
-
bioRxiv - Developmental Biology 2020Quote: Cumulative curve of the thymidine analog 5-Ethynyl-2’-deoxyUridine (EdU) incorporation is performed using Click-iT™ Plus EdU Alexa Fluor™ 647 Imaging Kit (ThermoFisher; C10640). Briefly ...
-
bioRxiv - Cell Biology 2021Quote: ... Samples were pre-concentrated on a C18 trap column (Acclaim PepMap 100; 100 μm × 2 cm, 5 μm, 100 Å; Thermo Fisher Scientific) within seven minutes at a flow rate of 30 μL/min with 0.1 % trifluoric acid and subsequently transferred to a Nano Viper C18 analytical column (Acclaim PepMap RSLC ...
-
bioRxiv - Cell Biology 2022Quote: ... siRNA oligonucleotides were obtained as pre-designed siRNAs as follows: MFF-sense strand: 5’-CGCUGACCUGGAACAAGGAdTdT-3’ for exon 2 30 (Ambion, Austin, TX, USA); DLP1-sense strand ...
-
bioRxiv - Biochemistry 2022Quote: ... of each sample were loaded by 20 μL of 0.1% formic acid in water at a pressure of 200 bar onto a trap column (100 μm × 2 cm, PepMap nanoViper C18 column, 5 μm, 100 Å; Thermo Fisher Scientific). The following mobile phases were used ...
-
bioRxiv - Microbiology 2022Quote: ... 5 μg of each RNA sample was incubated twice with 2 units of TURBO DNase (TURBO DNA-free Kit, Ambion, Austin, TX, USA) for 30 min at 37 °C ...
-
bioRxiv - Molecular Biology 2022Quote: ... 4ºC) and incubated with 50 μL of 5 μM DAF-FM (4-amino-5methylamino-2’,7’-dichlorofluorescein diacetate, Life Technologies, Eugene, OR, USA) diluted in 1X PBS for 30 min at 34 °C ...
-
bioRxiv - Neuroscience 2022Quote: ... free floating NAc sections were first washed (3 × 5 min) in 1x PBS containing 2% Triton X-100 (PBST) (Thermo Fisher, Waltham, MA). Sections were then blocked in 5% normal goat serum (NGS ...