Labshake search
Citations for Thermo Fisher :
2051 - 2100 of 10000+ citations for TRAP 5 amide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... Sections were then washed 3 x 5 min in PBS and incubated for 5 min in DAPI (Invitrogen, cat# D1306) 1:1000 in PBS ...
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bioRxiv - Bioengineering 2022Quote: ... Slides were dehydrated by sequential submersion in 100% ethanol (2× 5 min) and SafeClear II (2× 5 min, Fisher Scientific) before being mounted in Cytoseal 60 (ThermoFisher ...
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bioRxiv - Cell Biology 2022Quote: ... at 37°C in a 5% CO2 incubator with daily media changes and were passaged every 4-5 days using TrypLETM (ThermoFisher), following manufacturer’s recommendations ...
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bioRxiv - Cell Biology 2022Quote: ... Guide RNA primer sets A (KS40 5’-ACCGACCACAGAAACTGCGACTAG -3’ and KS41 5’-AACCTAGTCGCAGTTTCTGTGGTC-3’) and B (KS42 5’-CCGTCCCACTGGCACCGCTTTATG-3’ and KS43 5’-AAACATAAAGCGGTGCCAGTGGGA-3’) were phosphorylated with T4 polynucleotide kinase (ThermoFisher) at 37°C for 30 min and annealed by cooling down from 85°C to 25°C at 0.1°C/sec ...
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Reducing mitochondrial ribosomal gene expression does not alter metabolic health or lifespan in micebioRxiv - Cell Biology 2022Quote: ... Genotyping proceeded according to the ICS protocol (Forward primer Ef 4877 5’-GACCCACATAAGCAGGGAAGGAGATG-3’, reverse primer L3r 4879 5’-CAATCTCCTGAGAATGTAGCCCACCAT-3’, Invitrogen). The Mrpl54 knock-out allele generated a 402 base-pair (bp ...
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bioRxiv - Immunology 2022Quote: ... at a density of 5 million cells/mL and cultured at 37°C with 5% CO2 in RPMI 1640 containing glutamine (Invitrogen), supplemented with 10% FBS (Gibco) ...
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bioRxiv - Immunology 2022Quote: ... with siRNAs against SAMHD1 (sense RNA 5’-GCAGAUAAGUGAACGAGAUTT-3’, antisense RNA 5’-AUCUCGUUCACUUAUCUGCAG-3’) or the negative control #1 siRNA (Ambion). Three days after transfection with either siRNA or shRNA plasmids ...
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bioRxiv - Genomics 2022Quote: ... We removed the supernatant and then resuspended the pellet in 200 μl of FACS buffer (PBS supplemented with 5% BSA and 5 mM EDTA) supplemented with 1U/μl SUPERase.in (Invitrogen, AM2696). Spermatogonia were sorted according to (Kanatsu-Shinohara et al ...
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bioRxiv - Immunology 2022Quote: ... PCR of the precipitated product (5 ng) was performed using a reaction with 5 μL SyBR green (#A25742; ThermoFisher Scientific) and 0.5 μL of 10 μM primer listed in the Supplementary table 3 ...
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bioRxiv - Microbiology 2022Quote: ... The plates were fixed with 4% w/v formaldehyde for 15 min and stained with 5 μg/mL DAPI and 5 μg/mL CellMask deep red plasma membrane stain (Invitrogen) for 1 h at room temperature ...
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bioRxiv - Physiology 2022Quote: ... Total of 0.5–0.7 mL blood was collected from the heart and stored in 0.75 mL tubes containing 5 µL EDTA and 5 µL Halt protease and phosphatase inhibitor cocktail (100x, Thermo Scientific). The tubes were centrifuged for 10 minutes (2000 x G ...
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bioRxiv - Neuroscience 2022Quote: ... All AAVs were measured relative to standards with primers targeted to the ITRs (forward: 5′-GGAACCCCTAGTGATGGAGTT, reverse: 5′-CGGCCTCAGTGAGCGA) with the SYBR Green PCR Master Mix (ThermoFisher). qPCR was performed on the StepOnePlus real-time PCR system ...
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bioRxiv - Neuroscience 2022Quote: ... 2.5 μL of cDNA (12.5 ng) were added to 7.5 μL of a master mix containing 5 μL of Taqman (11732-020, Applied Biosystems) and 2.5 μL of nuclease-free water containing golden hamster’s primer pairs (Table S2) ...
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bioRxiv - Neuroscience 2022Quote: ... the sgRNA sequence targeting exon 1 of Faah (5’-CTGCAGGCTAGGCAAACC-3’) and a control sgRNA sequence (5’-CTGCAGGCTAGGCAAACCTTT-3’ were synthesized (Invitrogen) and cloned into the shuttle plasmid for adeno-associated viral (pAAV-FLEX-SaCas9-U6-sgRNA;Addgene #124844 ...
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bioRxiv - Plant Biology 2022Quote: ... chpre-MIR166A was amplified from genomic DNA of Cardamine Oxford ecotype using specific primers: FW 5’-GGGGACAAGTTTGTACAAAAAAGCAGGCTGGGAGGAAGGAAGGGGCTTTCT-3’ REV 5’-GGGGACCACTTTGTACAAGAAAGCTGGGTGCCCTAATTAAATTGAGAAGAAGG-3’ and cloned in pDONR221 Gateway vector by BP recombination (Invitrogen). pDONRP4_P1-ChSCRp (Di Ruocco et al ...
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bioRxiv - Microbiology 2023Quote: ... or alkaline phosphatase was added at a 1:2,000 dilutions for 1 h at 37ºC followed by adding TMB (3, 3, 5, 5′-tetramethylbenzidine) peroxidase substrate (Thermo Scientific) or p-nitrophenyl phosphate (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2023Quote: ... and primers (46) (5′-CAGAGATCGATCTGTTTCCTTGACACGCGTGCCACCATGTTCGTGTTCCTG-3′ and 5′-AATCTGTGTGCAGGGCGGCCGCTCAGGTGTAGTGCAGCTTCACG-3′) and cloned by using the Zero Blunt TOPO PCR Cloning Kit (ThermoFisher).
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bioRxiv - Cancer Biology 2022Quote: ... minced skin was incubated at 37°C for 3 – 5 hours in 5 ml of DMEM high glucose (#41965-039; Gibco) supplemented with 10 mg ml-1 collagenase (#C9891 ...
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bioRxiv - Developmental Biology 2022Quote: ... at 38.5°C overnight in sealed sterile vials containing 5% CO2 in air-equilibrated Medium 199 with Earle’s salts (Thermo Fisher), supplemented with 10% fetal bovine serum (Hyclone) ...
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bioRxiv - Microbiology 2023Quote: ... A549 cells were seeded in 96-well microtiter plates at a concentration of 5−104 cells per well for 24 h (37°C, 5% CO2) in DMEM (Gibco) supplemented with 10% FBS (Gibco ...
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bioRxiv - Synthetic Biology 2023Quote: ... to a final concentration of 5% and fetal bovine serum to a final concentration of 5% and 1% penicillin-streptomycin (Invitrogen) or Clear X9-Stem™ at 5% CO2 ...
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bioRxiv - Neuroscience 2023Quote: ... The blood was quickly collected with a syringe and transferred in 0.75 mL tubes containing 5 μL EDTA and 5 μL Halt protease and phosphatase inhibitor cocktail (100x, Thermo Scientific). Blood samples were centrifuged for 10 min at 2000 x g at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... The infected cells were resuspended in HL5c containing 5 U mL−1 of penicillin and 5 μg mL−1 of streptomycin (Invitrogen) to prevent extracellular bacterial growth ...
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bioRxiv - Evolutionary Biology 2022Quote: ... 1.5 µg of total RNA of each sex were subjected to 5’ and 3’ RACE with a GeneRacer kit (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... and incubated for 2-24hrs at 37°C 5% CO2 +/- myristoylated aPKC pseudosubstrate inhibitor (5 μM; Invitrogen; Product number 77749) in IMDM+0.1% BSA ...
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bioRxiv - Cancer Biology 2023Quote: ... and then washed twice with HBSS before incubating with staining solution (5 U/mL phalloidin AF568 [Invitrogen], 100 μg/mL concanavalin A AF488 [Invitrogen], 5 μg/mL Hoechst 33342 [ThermoFisher or Invitrogen] ...
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bioRxiv - Microbiology 2023Quote: ... PCR covering the virus S2M region was performed on cDNA samples for 40 cycles with primers HJ551-S2UTRF: 5’-CTCCAAACAATTGCAACAATC-3’ and HJ552-S2UTRR: 5’-GTCATTCTCCTAAGAAGCTATTAAAATC-3’ using the High Fidelity AccuPrime Taq DNA Polymerase (Invitrogen) following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... primers with upstream attB-regions suitable for BP-clonase-recombination (attB1: 5′-GGGGACAAGTTTGTACAAAAAAGCAGGCTTAACA-3′; attB2: 5′-GGGGACCACTTTGTACAAGAAAGCTGGGT-3′, Gateway Technology, Invitrogen). By same the method ...
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bioRxiv - Genetics 2023Quote: RNA was extracted from the stock of D388-WT and the sequence of the 5′ UTR was determined using a 5′ RACE system for rapid amplification of cDNA ends (Invitrogen) using IBV-specific oligonucleotides 5′-TGTCTGCTCACTAAAC-3′ for the reverse transcription step and 5′-AGAACGTAGCCCAACGC-3′ for the amplification of dC-tailed cDNA step ...
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bioRxiv - Cell Biology 2023Quote: Whole proteome and phosphopeptide samples were dissolved in 2% [v/v] ACN 0.1% [v/v] TFA and injected onto a C18 PepMap100-trapping column (0.3 x 5 mm, 5 μm, Thermo Scientific) connected to an in-house packed C18 analytical column (75 μm x 300 mm ...
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bioRxiv - Microbiology 2023Quote: ... or the same concentration of scrambled siRNA (sense, 5’- UUCUCCGAACGUGUCACGUTT -3’; antisense, 5’- ACGUGACACGUUCGGAGAATT -3’) purchased from Tsingke Biotechnology (Beijing, China) with Lipofectamine 3000 (Invitrogen) according to the manufacturer’s recommendations.
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bioRxiv - Cell Biology 2023Quote: ... MiniBAR-GFP sta-ble cell line was transfected with 25 nM of siRNAs targeting luciferase (5’-CGUACGCGGAAUACUUCGA-3’) or human Rab35 (5’-GCUCACGAAGAACAGUAAA-3’) using Lipo-fectamine RNAiMAX (Invitrogen), following the manufac-turer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... The cells were seeded in 6-well plates (2.25 x 10^5 cells per well) supplemented with 5 nM siRNA premixed with Opti-MEM Reduced Serum Medium (Gibco) and Lipofectamine RNAi Max Transfection Reagent (Thermo Fisher Scientific) ...
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bioRxiv - Bioengineering 2023Quote: ... the gels were washed with wash buffer three times for 5 min followed by permeabilization and blocking in 5% goat serum (Gibco), 1% BSA ...
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bioRxiv - Immunology 2023Quote: ... SFB736F (5′-GACGCTGAGGCATGAGAGCAT-3′)/SFB844R (5′-GACGGCACGGATTGTTATTCA-3′) were used in a 7500 Fast Real-Time PCR System (Life Technologies) to quantify SFB level in the feces ...
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bioRxiv - Microbiology 2024Quote: ... 5 µl of each sample was loaded on a C18 precolumn (300 µm inner diameter × 5 mm, Thermo Fisher Scientific) in a solvent made of 2% acetonitrile and 0.05% trifluoroacetic acid ...
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bioRxiv - Molecular Biology 2024Quote: ... boiled at 95°C for 5 mins and spun at 21 300 rcf for 5 mins prior to loading in NuPAGE Tris-Acetate gels (Invitrogen). Gels were run at 150 V for 1 h and transferred onto 0.2 µm PVDF membranes using the High MW setting on the Trans-Blot Turbo (Bio-Rad) ...
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bioRxiv - Molecular Biology 2024Quote: ... about 5 million K562 cells were transfected with 5 μg luciferase reporter plasmid using Neon™ NxT Electroporation System (Invitrogen). Cells were fixed after 24 hours and ChIP performed as described above ...
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bioRxiv - Microbiology 2024Quote: ... GP2-293 cells were seeded at 5×106 on 10-cm plates and transfected 24 h later with 5 µg of LUJV-flag/LUJV-flag-mut and 5 µg Luciferase using Lipofectamine 2000 (Invitrogen). Cells’ media were replaced 5 h later to full medium ...
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bioRxiv - Cell Biology 2023Quote: ... Peptides were trapped on a µPrecolumn C18 PepMap100 (5 μm, 100 Å, 300 μm×5 mm, ThermoFisher Scientific, Reinach, Switzerland) and separated by backflush on a C18 column (5 μm ...
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bioRxiv - Microbiology 2020Quote: ... 20 μl of 5 mg/ml glycogen (Ambion), and 1 ml of cold isopropanol ...
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bioRxiv - Biophysics 2021Quote: ... wash with 5 mL DPBS (Thermofisher, cat. # 14190144) and aspirate ...
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bioRxiv - Cancer Biology 2021Quote: ... EdU 10μM (5-ethynyl-2’-deoxyuridine, ThermoFisher Scientific) diluted in fresh medium was added in the well for 10 minutes (MIA PaCa-2) ...
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bioRxiv - Cancer Biology 2021Quote: ... supplemented with 5% fetal calf serum (Gibco #16030074) and 200 nM 12-Otetradecanoyl phorbol-13-acetate (Sigma #16561-29-8) ...
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bioRxiv - Molecular Biology 2020Quote: ... and blocking with 5× Denhardt’s solution (Thermo Scientific) for 1 h at 37 °C ...
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bioRxiv - Cell Biology 2019Quote: ... was supplemented with 5% Horse Serum (Life Technologies), 10ug/ml Insuline ...
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bioRxiv - Cell Biology 2020Quote: ... 5 µl of fluorescent microbeads (FluoSpheres; Molecular Probes) were incubated with 0.1 mg/ml PLL-PEG on a rotator for 1 h at 4°C prior to mixing with the gel mixture ...
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bioRxiv - Genetics 2021Quote: ... and 5% heat-inactivated FBS (Thermo Fisher Scientific). All cells were cultured at 25°C and were free from mycoplasma contamination.
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bioRxiv - Genomics 2021Quote: ... containing 10% FBS and 5% pen/strep (ThermoFisher). Cells were counted with a hemocytometer and Seq-Well S3 was performed as described below43 ...
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bioRxiv - Cell Biology 2020Quote: ... Molecular Probes)/PBS solution or 5 mg/ml Hoescht (Invitrogen)/PBS solution was carried out for 15 min at RT ...