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Citations for Thermo Fisher :
1901 - 1950 of 10000+ citations for 7 Chloro 1 3 dihydro imidazo 4 5 c pyridin 2 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... proteins were separated by one-dimensional gel electrophoresis (4–12% NuPAGE Bis-Tris Gel; Invitrogen), and the entire lane of a Coomassie blue-stained gel was cut into 23 slices ...
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bioRxiv - Molecular Biology 2019Quote: ... One fourth of the eluted samples was separated on 4-12% NuPage gels (Life Technologies) and the gels stained with coomassie blue (ProtoBlue ...
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bioRxiv - Neuroscience 2022Quote: Tissue cultures (n=3) were transferred as one sample into 250 μl RNAlater (ThermoFisher, Cat# AM7020) and stored at −20 °C ...
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bioRxiv - Molecular Biology 2024Quote: ... one-step RT-qPCR was performed using a QuantStudio 3 Real-Time PCR System (Applied Biosystems) using the Luna Universal One-Step RT-qPCR Kit (NEB) ...
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bioRxiv - Neuroscience 2020Quote: ... OFC sections were reacted with the primary antibody for rabbit met-enkephalin (1:400, Abeam ab22620) for 24h at 4°C and Alexa-fluor 488 goat anti rabbit secondary antibody (Invitrogen, 1:400) for 24 hours at 4°C ...
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bioRxiv - Molecular Biology 2021Quote: ... the samples were washed with PBS and incubated in the dark for 1 h at 4 °C with secondary antibody (Alexa Fluor-568-conjugated anti-mouse antibody, 1:750, Thermo Fisher Scientific). Finally ...
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bioRxiv - Molecular Biology 2022Quote: ... at 30 V for 1 hour followed by100 V for a further hour at 4°C in 1× NuPAGE Transfer Buffer (Thermo Fisher Scientific) supplemented with 0.1 g/l of SDS (Sigma-Aldrich ...
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bioRxiv - Microbiology 2020Quote: ... (alone or in combination) in IF washing buffer overnight at 4°C: anti-ZO-1 rabbit polyclonal (1:200) (Thermo Fisher Scientific), anti-E-cadherin rabbit monoclonal (1:200 ...
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bioRxiv - Cell Biology 2019Quote: ... Collagen/laminin coating was accomplished by overnight incubation of the plates rocking at 4°C with a 1:1 mixture of laminin (10 µg/ml ThermoFisher 23017-015) and collagen (10 µg/ml Sigma C8919 ...
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bioRxiv - Molecular Biology 2021Quote: ... Cell lysates containing 1 mg of protein were incubated with anti-HA antibody (1:100) for 1 h at 4 °C followed by incubation with Protein G Dynabeads (Thermo Fisher Scientific) for 1 h at 4 °C ...
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bioRxiv - Microbiology 2022Quote: ... the Transwell inserts were incubated with one of the following primary Abs in IF wash buffer overnight at 4°C: anti-ZO-1 rabbit polyclonal (1:200) (40-2200, Thermo Fisher Scientific), anti-E-cadherin rabbit monoclonal (1:200 ...
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bioRxiv - Neuroscience 2023Quote: ... concentration 1:100) for 24 h at 4°C followed by labeling with goat anti-rabbit Alexa fluor 488 (A11034, Invitrogen, 1:1000) as a secondary antibody for 3 h at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... 10% Donkey Serum, 1% Triton-100, 1h) followed by primary (overnight, 4°C) and secondary antibodies (1-2h, Jackson ImmunoResearch or ThermoFisher Scientific) incubation ...
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bioRxiv - Cell Biology 2023Quote: ... and 20% sucrose-PBS and then incubated overnight at 4°C in a 1:1 solution of 20% sucrose and OCT compound (Fisher Scientific: #23730571). The next day ...
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bioRxiv - Cell Biology 2023Quote: ... Day 10 post plating (7 days after air lift) cells were fixed in 4% PFA and stained for tight junction protein ZO-1 (Thermo Fisher Cat# 61-7300). Randomized Z stack images were taken at 20x in 3 different places of each transwell for three transwells and imported into ImageJ ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Cellular debris was removed by centrifugation at 5,488x g for 40 min at 4°C (Heraeus™ Megafuge™ 40R) and incubated for 2 h with 500 μl HisPurTM Cobalt Resin (89964, ThermoFisher Scientific) at 4°C ...
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bioRxiv - Neuroscience 2019Quote: ... Brains were immersed in 30% sucrose at 4°C until sunk and flash frozen with cold 2-methylbutane (Fisher Scientific, Hampton, NH, USA). Coronal sections of the pregenual mPFC were obtained at 35 µm using a cryostat (Leica CM3050 S ...
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bioRxiv - Cell Biology 2020Quote: ... 1000 μg of total protein was incubated overnight at 4 °C with 2 μg α-GFP (Life Technologies/ThermoFisher Scientific, Carlsbad, CA, USA) and 20 μL of a 50% slurry of sepharose Protein A (Rockland Immunochemicals ...
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bioRxiv - Cell Biology 2022Quote: ... washed with PBS and stained overnight at 4 °C with 20 μg/mL of LAMP-2 primary antibody (Thermo Fisher Scientific, MA, USA) in 1 % BSA (in PBS ...
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bioRxiv - Microbiology 2023Quote: ... [72] at 4 °C overnight followed by staining with 2 µg/ml of secondary AlexaFluor647-conjugated Donkey anti-human IgG antibody (ThermoFisher Scientific, OR, USA) at room temperature for 45 min ...
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bioRxiv - Genomics 2023Quote: ... was used and samples were run for 2 hours 15min at 80V at 4 °C in 1X tris-acetate running buffer (Thermo Fisher Scientific, LA0041) except for the loading control which was run for 90 minutes ...
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bioRxiv - Physiology 2022Quote: ... Brains were quickly transferred to 10 μL of the same saline in a 200 μL microcentrifuge tube kept at 2-3°C in a Boekel Scientific TropiCooler Benchtop Incubator (model 260014, Fisher Scientific, Ottawa, ON, Canada), and once 30 brains were collected (2-2.5 min per brain ...
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bioRxiv - Immunology 2020Quote: ... 25mM HEPES (2(−4-(2-hydroxyethyl)-1-piperanzinyl) ethansulfonacid) and supplemented with 10% (v/v) fetal bovine serum (FBS; Gibco, ThermoFisher Scientific), 10.000 U/mL (v/v ...
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bioRxiv - Neuroscience 2021Quote: ... slices were incubated with 4’,6-diaminodino-2-phenylindole (DAPI, Life Technologies D-21490, 1:2000) for 15 min ...
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bioRxiv - Neuroscience 2020Quote: ... The nuclear dye 4’,6-diamidino-2-phenylindole (DAPI) at 1 l ng/ml (Molecular Probes) was added to visualise all cells ...
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bioRxiv - Physiology 2020Quote: ... Cell nuclei were stained with 4’,6-diamidino-2-phenylindole (DAPI; 1:10,000; ThermoFisher, Table S13). Confocal images were acquired using an inverted laser scanning confocal microscope (Nikon Eclipse C1) ...
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bioRxiv - Neuroscience 2019Quote: ... Nuclei were stained with DAPI (4’,6-diamidino-2-phenylindole, 1:500, Molecular Probes, Eugene, USA) and images were obtained using a scanning confocal inverted microscope (TCS ...
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bioRxiv - Immunology 2021Quote: ... Nuclei were counterstained with 1/2500 4’,6-diamidino-2-phenylindole (DAPI, Invitrogen, ThermoFisher Scientific, USA). The cover slips were fixed in fluorescence mounting medium (Fluoromount-G ...
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bioRxiv - Immunology 2021Quote: ... Nuclei were counterstained with 1/2500 4’,6-diamidino-2-phenylindole (DAPI, Invitrogen, ThermoFisher Scientific, USA). The cover slips were fixed in fluorescence mounting medium (Fluoromount-G ...
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bioRxiv - Neuroscience 2021Quote: ... and 1 mM of the serine protease inhibitor 4-(2-aminoethyl) benzenesulfonyl fluoride hydrochloride (ThermoFisher Scientific). The samples were further ruptured using a syringe and centrifuged at 14,000g for 30 min at 4°C ...
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bioRxiv - Neuroscience 2021Quote: ... and 1 mM of the serine protease inhibitor 4-(2-aminoethyl) benzenesulfonyl fluoride hydrochloride (ThermoFisher Scientific). The samples were sonicated and centrifuged at 14,000g for 30 min at 4°C ...
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bioRxiv - Cell Biology 2019Quote: ... DNA is stained with 1 mg/ul 4’,6-diamidino-2-phenylindole (DAPI) (ThermoFisher, Carlsbad, CA) 1/10,000 in PBS for 5 minutes and Vectashield mounting medium is applied (Vector Labs ...
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bioRxiv - Cell Biology 2019Quote: ... DNA is stained with 1 mg/ul 4’,6-diamidino-2-phenylindole (DAPI) (ThermoFisher, Carlsbad, CA) 1/10,000 in PBS for 5 minutes and Vectashield mounting medium is applied (Vector Labs ...
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bioRxiv - Bioengineering 2019Quote: ... Sections were also counterstained with 4′,6-diamidino-2-phenylindole (DAPI; 1:1000; Invitrogen, cat. #D1306). Immunocytochemical images were acquired with a confocal microscope (Olympus FV1000 Laser Scanning Confocal ...
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bioRxiv - Cell Biology 2019Quote: ... also confirmed by 4′,6-diamidino-2-phenylindole fluorescent dye (DAPI, 1:1000, Molecular Probes, USA) staining.
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bioRxiv - Immunology 2021Quote: ... Chamberslides were then incubated in 4’,6-Diamidino-2-phenylindole dihydrochloride (DAPI) (1:5,000, ThermoFisher Scientific) in 1x PBS for 5 min ...
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bioRxiv - Immunology 2020Quote: ... cell nuclei were counterstained with 4′,6-diamidino-2-phenylindole (DAPI; Invitrogen, Karlsruhe, Germany, 1:100) for 30 min at RT ...
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bioRxiv - Cancer Biology 2022Quote: ... and 4′6-diamidino-2-phenylindole (DAPI, nuclei staining, 1:10000; Cat. no. D1306, Life Technologies) diluted in PBS for 30 min at RT ...
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bioRxiv - Cancer Biology 2022Quote: ... Slides then were counterstained with 4′,6-diamidino-2-phenylindole (DAPI) or with YOYO-1 (ThermoFisher). When HPV probe signal co-localized with YOYO-1 signal detecting DNA at 63x magnification ...
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bioRxiv - Bioengineering 2022Quote: ... counterstained with 1 μg/mL of 4′,6-diamidino-2-phenylindole (DAPI; Thermo Fisher Scientific, #D1306) for 30 minutes ...
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bioRxiv - Cell Biology 2022Quote: ... 25 mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES; Thermo Fisher Scientific, Cat. no. 15630-080,) and 1% penicillinstreptomycin (Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2022Quote: ... 0.1 M 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) buffer (pH = 8.5) (ThermoFisher Scientific, Waltham, MA), 4-benzoylbenzyl-trimethylammonium chloride (PLPP ...
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bioRxiv - Immunology 2023Quote: ... Slides were washed and stained with 4’,6-diamidino-2-phenylindole (DAPI, Invitrogen, D3571, 1:4000) in PBS for 10 min at room temperature ...
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bioRxiv - Immunology 2024Quote: ... These were transfected at a 1:2:4 w/w ratio using Lipofectamine 3000 (Invitrogen #L3000001), according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: BDA was visualized with fluorophore-conjugated streptavidin (Thermo Fisher Scientific, Table 2; 1:1,000 for 3 h). The reaction was enhanced using the biotinylated tyramine (BT)-glucose oxidase (GO ...
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bioRxiv - Microbiology 2022Quote: Anti-ORF8 mAb (#1-3-2) was coupled to aldehyde/sulfate latex beads (Thermo Fisher Scientific A37304). The antibody was mixed with the latex beads and shaken at room temperature overnight ...
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bioRxiv - Cell Biology 2021Quote: ... cells were incubated for overnight with primary antibody at 4 degrees and further incubated with secondary antibodies (1:500) for 1 hour followed by 4′,6-diamidino-2-phenylindole (DAPI) or phalloidin 488 (1:400, Thermo Fisher, A12379) staining ...
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bioRxiv - Molecular Biology 2021Quote: ... The temperature was maintained at 37 °C by a thermostatic water circulator (NESLAB RTE-7; Thermo Scientific). The samples were prepared in 20mM HEPES ...
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bioRxiv - Microbiology 2020Quote: ... 4 μl of 5 mg/ml linear acrylamide (Ambion), 600 μl of preheated (65°C ...
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bioRxiv - Cell Biology 2022Quote: ... and Ca2+-indicator Fluo-4/AM (5 μM, Invitrogen) in the presence of Pluronic F-127 (0.02% ...