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Citations for Thermo Fisher :
1901 - 1950 of 8295 citations for Rat TUT1 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... heavy chain plasmid DNA and light chain plasmid DNA were diluted in 1.5ml Opti-Plex Complexation Buffer (Invitrogen) before mixing with 80µl ExpiFectamine 293 Reagent (Invitrogen ...
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bioRxiv - Neuroscience 2021Quote: ... and total DNA amounts of 5μg (2.5μg donor vector plus 1.25μg of each TALENS arm plasmid) or 2μg (1μg piggybac vector plus 1μg of transposase plasmid) were combined with Lipofectamine Stem (ThermoFisher, STEM00003) in Opti-MEM (ThermoFisher 11058021) ...
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bioRxiv - Neuroscience 2020Quote: ... Large quantities of purified plasmids were obtained using the PureLinkTM HiPure Plasmid Filter Midiprep kit (Thermo Fisher Scientific). The cloned sequences of ItypOrco ...
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bioRxiv - Immunology 2019Quote: ... plasmids were treated with DpnI to remove non-mutated parental plasmids and cloned into Stbl2 cells (Life Technologies) using the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2019Quote: ... 5 × 104 E14Tg2a cells were transfected with 100 ng of reporter plasmids (pGL4.10[luc2] and their derivatives) and 1 ng of pGL4.70[hRluc] control plasmid using Lipofectamine 2000 (Invitrogen) in 96-well plates ...
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bioRxiv - Biophysics 2019Quote: ... were selected for their suitability for transformation with a plasmid containing ampicillin resistance (pRSET/EmGFP plasmid; Invitrogen, UK).
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bioRxiv - Neuroscience 2019Quote: ... human Ad5 genomic plasmid (pBHGfrtΔE1,3FLP; Microbix) and each pDC511-LRRK2 shuttle plasmid were introduced by calcium phosphate (Invitrogen) transfection into stable Tet-On-inducible E2a cells (E2T cells ...
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bioRxiv - Cancer Biology 2020Quote: ... the lentiviral plasmids and packaging plasmids were transfected into lentiX-293T cells using Lipofectamine 2000 (Invitrogen, Carlsbud, CA) and lentivirus-containing supernatants were harvested after 3 days ...
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bioRxiv - Cell Biology 2019Quote: ... Plasmids were then extracted and purified using the PureLink HiPure Plasmid Maxiprep Kit (Thermo Fisher Scientific, Cat# K210006) for downstream experiments ...
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bioRxiv - Molecular Biology 2024Quote: ... Plasmid DNA was isolated using the GeneJET Plasmid Miniprep Kit (Cat #K0503; Thermo Fisher Scientific, MA, The US). Isolated plasmids were prepared for transformation by linearizing with EcoRI restriction enzyme according to the supplier’s instructions (NEB ...
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bioRxiv - Microbiology 2024Quote: Isolation of plasmid DNA was carried out with Genejet Plasmid Miniprep kit (Thermo Fisher Scientific, Pittsburgh, PA, USA), according to the manufactureŕs instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... 100 ng firefly luciferase plasmid and 112.5 ng GFP or different eIF1 plasmids using Lipofectamine 2000 (Thermo Fisher). After 24 hours ...
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bioRxiv - Cell Biology 2024Quote: ... cells were transfected with 750 ng of plasmid purified using the GeneJET plasmid miniprep kit (Thermo Fisher Scientific). Briefly ...
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bioRxiv - Cell Biology 2023Quote: ... The confirmed plasmid was prepared in large quantities using the PureLink HiPure Plasmid Midiprep Kit (Thermo Fisher Scientific). The plasmid concentration was measured using a NanoDrop 2000 Spectrophotometer (Thermo Fisher Scientific) ...
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bioRxiv - Synthetic Biology 2023Quote: ... 10 µL of plasmid-lipofectamine mixture containing 75 ng plasmid and 165 ng Lipofectamine 2000 (Invitrogen, 11668-019) in Opti-MEM (Gibco ...
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bioRxiv - Molecular Biology 2023Quote: ... Plasmid transfections were performed using 2 μg plasmid per 3.5-cm well plates using Lipofectamine 2000 (Life Technologies) transfection reagent according to the manufacturer’s recommendations at 48 hours before harvest ...
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bioRxiv - Genomics 2023Quote: ... Colonies were selected and plasmid DNA was extracted using The PureLink™ Quick Plasmid Miniprep Kit (Thermofisher Scientific) and sent for Sanger sequencing to identify the integrity and quality of the inserted enhancer fragment ...
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bioRxiv - Systems Biology 2023Quote: ... coli (High Efficiency) cells and the plasmid DNA was purified using the PureLink™ Plasmid Purification Kit (Invitrogen). Three oligonucleotide pools were cloned separately and pooled together to acquire the final 7mer library ...
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bioRxiv - Molecular Biology 2023Quote: ... All plasmids containing the promoter regions were ordered via gene synthesis as Gateway-compatible donor plasmids (ThermoFisher Scientific). Promoter sequence and expression cassette from donor constructs pEntry.P4P1R.EF1a and pEntry.MiniCas9V2 (Maria et al. ...
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bioRxiv - Synthetic Biology 2023Quote: ... and the pWS082-CAN1 sgRNA plasmid (Table S1) was extracted using the GeneJet Plasmid miniprep kit (ThermoFisher Scientific). Accuracy of the cloning was confirmed through DNA sequencing.
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bioRxiv - Cancer Biology 2024Quote: ... The BUB1 plasmids or siRNA with plasmids were transfected with Lipofectamine 2000 (Catalog No. 11668500, Thermo Fisher Scientific) per manufacture’s protocol.
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bioRxiv - Bioengineering 2020Quote: ... using PureLink Quick Plasmid Miniprep Kit (Invitrogen) and were sequence verified ...
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bioRxiv - Synthetic Biology 2021Quote: ... 500ng of T7 calmodulin plasmid (Invitrogen, #454002) was also used in the following cell free expression reactions ...
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bioRxiv - Cell Biology 2020Quote: ... Plasmid DNA and Plus reagent (Invitrogen # 11514015) were mixed at 1:1 and incubated for 5 minutes ...
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bioRxiv - Biophysics 2020Quote: ... and PureLink HiPure Plasmid Midiprep Kit (Invitrogen). The DNA sequences were read by dye terminator cycle sequencing using the BigDye Terminator v3.1 Cycle Sequencing Kit (Applied Biosystems).
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bioRxiv - Microbiology 2021Quote: ... and cloned into the pBAD plasmid (ThermoFisher), as previously described (46) ...
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bioRxiv - Molecular Biology 2019Quote: ... Plasmids were transfected using Lipofectamin 2000 (Invitrogen) according to the manufacturer’s instructions for 48 hours before harvesting ...
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bioRxiv - Molecular Biology 2019Quote: ... Plasmids were delivered using Lipofectamine 3000 (Invitrogen) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... plasmids by Lipofectamine 3000 (Invitrogen, L3000015, USA). The relative hACE2 quantity was evaluated through mRNA expression level using following primers ...
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bioRxiv - Evolutionary Biology 2022Quote: ... and cloned into the pCR8 plasmid (Invitrogen), then transferred using the Gateway system into the pBPGUw (Pfeiffer et al. ...
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bioRxiv - Molecular Biology 2020Quote: ... Plasmids were transformed into BL21(DE3)(Invitrogen). Bacterial cultures were grown to an OD600 of 0.6 at 37 °C ...
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bioRxiv - Developmental Biology 2019Quote: ... later sub-cloned in pet28a plasmid (Invitrogen) in order to produce 6 His-tagged proteins.
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bioRxiv - Bioengineering 2019Quote: ... derived from plasmid pYES1L (Thermo Fisher Scientific), was inserted into pRM01 ...
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bioRxiv - Immunology 2019Quote: ... into a modified pMT/BiP plasmid (Invitrogen) that encodes for a C-terminal strep-tag sequence ...
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bioRxiv - Synthetic Biology 2020Quote: ... Plasmid mixes were prepared in Optimem (Gibco) and mixed with 0.75 μl of LF2000 and added dropwise to cells ...
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bioRxiv - Cell Biology 2019Quote: ... for plasmid DNA or Lipofectamine RNAiMAX (Invitrogen) for siRNA ...
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bioRxiv - Cancer Biology 2020Quote: ... reaction between pDONR221 plasmid (ThermoFisher Scientific, 12536017) containing V5-tagged wild-type or mutant CBL cDNA and lentiviral destination plasmid pRRL-SFFV-IRES-GFP or -dTomato (kindly provided by Christopher Baum and Axel Schambach ...
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bioRxiv - Genomics 2019Quote: ... Plasmids were transformed into GT115 E.coli (Invitrogen) and DNA isolated using PureYield Plasmid Midiprep system (Promega) ...
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bioRxiv - Bioengineering 2019Quote: ... and GeneJET Plasmid Miniprep (Thermo Fisher Scientific) or any other commercially available PCR and plasmid purification kits.
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bioRxiv - Bioengineering 2021Quote: The dual expression plasmid pFastBacDual (Invitrogen, USA) was purchased from Thermo Fisher Scientific China Co. ...
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bioRxiv - Microbiology 2020Quote: The following plasmids were used: pcDNA3 (Invitrogen), pSUMO1 (pcDNA3-HA-SUMO1 was a gift from Junying Yuan ...
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bioRxiv - Microbiology 2021Quote: ... cloned into pJet plasmid (Thermo Scientific, USA), and a mutation was verified by DNA sequencing ...
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bioRxiv - Molecular Biology 2020Quote: ... and envelope plasmid in OptiMEM (Life Technologies) for 5 to 6 hours (PEI:total pDNA μg ratio 2:1) ...
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bioRxiv - Cell Biology 2021Quote: ... The d1-eGFP plasmid was from Invitrogen, and constructed by cloning the d1-eGFP synthetic gene into a pcDNA3.3-TOPO vector backbone.
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bioRxiv - Cell Biology 2021Quote: ... pLP/VSVG DNA plasmids (Thermo Fisher Scientific). Cell culture medium containing lentivirus particles was collected after 48 and 72 h ...
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bioRxiv - Cell Biology 2020Quote: ... Control plasmid pcDNA3.1 was purchased from Invitrogen. DDI2-V5-His (wild type ...
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bioRxiv - Cell Biology 2022Quote: ... and pCMV-Renilla luciferase plasmid (Thermofisher #16153). Cells were transiently transfected with PTPN14 V5 tagged expression constructs (Liu et al. ...
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bioRxiv - Cell Biology 2022Quote: ... Plasmids were transfected using Lipofectamine 3000 (ThermoFisher) per manufacturer instructions ...
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Nerve pathology is prevented by linker proteins in mouse models for LAMA2-related muscular dystrophybioRxiv - Neuroscience 2022Quote: ... plasmids were performed with Lipofectamin 2000 (Invitrogen) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... into the pENTR-D-TOPO plasmid (Invitrogen) and perform an LR clone reaction to transfer it into the destination lentiviral vector SIN-cPPT-PGK-Gateway-WPRE-miR124T plasmid ...