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Citations for Thermo Fisher :
2001 - 2050 of 10000+ citations for 6H Dibenzo b d pyran 3 pentanaminium 6a 7 10 10a tetrahydro 1 hydroxy N N N 6 6 9 hexamethyl 6aR trans 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2021Quote: ... The UHPLC was equipped with a trap column (Acclaim PepMap 100, 75 μm x 2 cm, C18, P/N 164535, Thermo Fisher Scientific) and a 50 cm analytical column (Acclaim PepMap 100 ...
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bioRxiv - Biophysics 2021Quote: ... This step was performed in order to break the existing cadherin junctions and allow the blocking antibodies (anti N-Cadherin antibody: LEAF™ Purified anti-human CD325, #350804, Biolegend, USA; anti E-Cadherin antibody: CD324 #16-3249-82, Invitrogen, USA) to bind to their respective epitopes ...
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bioRxiv - Molecular Biology 2021Quote: ... Genes were cloned into modified pRSF1-Duet expression vector with a TEV-cleavable N-terminal 14-histidine tag (39) using New England Biolabs and Anza (Thermo Fisher Scientific) enzymes ...
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bioRxiv - Biochemistry 2021Quote: SARS-CoV-2 RBD proteins for SPR binding assays (residues 328-531 of S protein from GenBank NC_045512.2 with N-terminal signal peptide and C-terminal thrombin cleavage site-TwinStrep-8xHis-tag) were expressed in Expi293F (Thermo Fisher Scientific) cells at 37°C and 8% CO2 ...
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bioRxiv - Immunology 2021Quote: ... and used to amplify cDNA corresponding to the N and C termini of the spike protein and the plasmid backbone using Platinum SuperFi II DNA Polymerase (Thermofisher Cat# 12368010) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... and used to amplify cDNA corresponding to the N and C termini of the spike protein and the plasmid backbone using Platinum SuperFi II DNA Polymerase (Thermofisher Cat# 12368010) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: Tumor samples with sufficient high-quality RNA (n=5) were analyzed on the Affymetrix GeneChip Human Genome U133 Plus (v.2.0) Array (Affymetrix, Santa Clara, USA) at the Microarray Department of the University of Amsterdam ...
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bioRxiv - Biochemistry 2022Quote: N-terminal amino acid sequencing for the eOD-GT8 60mer was performed using the Procise Protein Sequencing System (Applied Biosystems, CA, USA). The protein band corresponding to the eOD-GT8 60mer (~50 kDa ...
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bioRxiv - Cell Biology 2022Quote: Banked adipose-derived stem cells (ADSCs) were purchased from CellTex Therapeutics (CellTex, catalog # N/A) and cultured in growth media containing DMEM-F:12 (Gibco, catalog # 11320033) with 10% Fetal Bovine Serum (FBS ...
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bioRxiv - Biochemistry 2024Quote: ... Dried samples were redissolved with 300 μL of 0.1% TFA in H2O and further fractionated using high-pH reversed-phase peptide fractionation kits (ThermoFisher, P/N 84868) following the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2022Quote: ... One-step real-time RT-PCR assays targeting SARS-CoV-2 nucleocapsid (N) coding region and subgenomic RNA were run on ABI 7900HT Sequence Detection Systems (Thermo Fisher Scientific), as previously reported25.
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bioRxiv - Neuroscience 2022Quote: LC-MS/MS analyses of purified and desalted peptides were performed on a Dionex UltiMate 3000 n-RSLC system (Thermo Fisher Scientific), connected to an Orbitrap FusionTM TribridTM mass spectrometer (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2022Quote: COCs were collected and analyzed after 24 h of IVM by means of ROS content (n=60; minimum of 20 per group) with CellROX® Green Reagent (CRG) (Thermo Fisher) and MMP (n=60 ...
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bioRxiv - Neuroscience 2024Quote: ... Samples were first concentrated over an Acclaim PepMap trap column (5.0 µm C18-coated particles, 0.5 cm * 300 µm ID, ThermoFisher Scientific P/N 160454), then bound onto a PepSep XTREME column (1.5 µm C18-coated particles ...
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bioRxiv - Bioengineering 2024Quote: ... which in turns intercalates with nucleic acids and emits red fluorescence detectable by microscopy.38 Frozen tissue sections from ATA samples (n=4 per group) were stained with DHE (10µM; D1168, Invitrogen; Carlsbad, CA, USA) in a dark humidified incubator at 37◦C for 20 minutes ...
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bioRxiv - Biochemistry 2024Quote: ... hamster (Syrian Hamster Isoform X1 – UniProt: A0A1U8BWQ2) and macaque (Macaque – UniProt: F6SVR2) with an N-terminal cMYC-tag were ordered to GeneArt (Thermo Fisher Scientific) and cloned into a phCMV backbone (GeneBank ...
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bioRxiv - Biochemistry 2024Quote: ... with a replacement in the N-terminal domain loop 8 (139CQKRDGPH146 to 139AEAG142, designated as rA3GR8) was constructed in the pET-sumo vector (Thermo Fisher Scientific). This construct generated a SUMO fusion with an N-terminal 6xHis tag and a PreScission protease cleavage site ...
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bioRxiv - Biochemistry 2024Quote: ... and cloned into a pRK5-derived plasmid with a C- or N-terminal FLAG tag using the gateway vector conversion system (Invitrogen, cat # 11828029). All gene constructs were verified by DNA sequencing.
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bioRxiv - Neuroscience 2024Quote: ... NPC were plated on poly- L-ornithine/laminin-coated flasks at 50’000 cells/cm2 in2µg/ml laminin-supplemented neural expansion medium (DMEM/F-12 + Glutamax, Gibco®; 1x N-2 supplement, Gibco®; 1x B27 supplement without vitamin A, Gibco® ...
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bioRxiv - Plant Biology 2024Quote: ... Contents of C and N were determined from 5 mg of tissue powder on a Flash 2000 Elemental Analyzer (Thermo Fisher Scientific), and contents of S were determined from 20 mg of tissue powder mixed with 5 mg of tungsten trioxide on an elemental PYRO cube analyzer (Elementar) ...
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bioRxiv - Cell Biology 2024Quote: ... cells were seeded onto coated 35 mm glass bottom dishes with a 20 mm glass aperture (Fisher Scientific D35-20-1.5-N). HEK293A cells were seeded onto dishes that had been coated with 10 µg ECL cell attachment matrix (Sigma 08-110 ...
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bioRxiv - Cell Biology 2024Quote: ... Antibody-mediated inhibition of hNR-EVs was performed with a commercial antibody that recognizes an extracellular topological domain of PLP1 at its N terminus (AA 36 to 85, Invitrogen, PA5-40788) incubating hNR-EVs with anti-PLP1 1.25 ug/ml for 15 min ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were separated by the sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) at 150V for 2h using NuPAGE 2-(N-morpholino)ethanesulfonic acid SDS running buffer (NP0002, Thermo Fisher Scientific), NuPAGE Antioxidant (NP0005 ...
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bioRxiv - Cancer Biology 2023Quote: ... and the total protein concentration was determined using a Pierce Reducing Agent Compatible BCA kit (RAC-BCA, Thermo Scientific, P/N 23252) following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... Full-length genes of human RNGTT and CMTR1 were cloned into pMlink vector containing a 4×ProteinA tag at the N-terminal and were transfected to Expi293F cells (Thermo Fisher Scientific) when cell density is 2.5× 106 /ml ...
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bioRxiv - Molecular Biology 2023Quote: ... The purified MuSCs from each replicate were stained for viability with calcein-AM and ethidium-homodimer1 (P/ N L3224 Thermo Fisher Scientific). scRNA libraries were generated using the GemCode Single-Cell Instrument (10x Genomics ...
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bioRxiv - Cancer Biology 2023Quote: Ewing sarcoma A673 (ATCC CRL-1598) and neuroblastoma SK-N-AS (ATCC CRL-2137) cell lines were grown in DMEM (Gibco, #31966-021) supplemented with 10% (v/v ...
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bioRxiv - Microbiology 2023Quote: ... Appropriate correction factors for the individual TMT channels for both lysine side-chain labelling and peptide N-terminal labelling as per the TMT-6plex kits used (Thermo Fisher Scientific) were configured into the database search ...
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bioRxiv - Developmental Biology 2023Quote: ... both diluted 1:50 in DMEM/F-12 or with 20 ug/mL recombinant truncated vitronectin (VTN-N) (Thermo Fischer Scientific, A31804) diluted in DMEM/F-12 (Gibco, 21331-020) for 1 hr at room temperature ...
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bioRxiv - Cancer Biology 2023Quote: ... Total RNA was isolated from MG63 lysates (n = 2 per treatment condition) using the PureLink RNA Mini kit (12183018A, Thermo Fisher Scientific) with DNase set (12185010 ...
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bioRxiv - Cell Biology 2023Quote: Liquid chromatography separation was performed at a flow rate of 300 nL/mL on a C18 pre-column (Acclaim PepMapTM 100, 75 μm×2 cm, nanoViper, P/N 164946, ThermoFisher Scientific Incorporation) followed by analytical column (Acclaim PepMapTM RSLC C18 ...
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bioRxiv - Cancer Biology 2023Quote: Wild-type and mutant (C139A) USP39 with a Flag-tag at its N-terminal were cloned into the pLenti6/V5 lentiviral vector (Invitrogen, California, USA). Specific shRNA oligonucleotides targeting USP39 were cloned into the pLL3.7 lentiviral vector (Addgene) ...
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bioRxiv - Genetics 2023Quote: ... The optimized and minimum required protocol for organoid hepatic isolation consisted of rinsing the fresh tissue in Phosphate Buffered Saline (PBS)/N-acetylcysteine (NAC) once and then transferring it to a tube filled with Advanced DMEM/F12 (Gibco; 12634-010), mincing the tissue into small fragments ...
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bioRxiv - Cell Biology 2023Quote: ... at a density of 107 cells in 19 ml of medium for dual-proteomics or at 1.5 x 104 cells per well in 96-well plates (Nunc, cat n° 164590) in 0.2 ml of medium for MTT ...
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bioRxiv - Immunology 2022Quote: ... which was created via the in vitro T7-DNA-dependent RNA transcription of a linearized DNA molecule containing the target region of the N gene full-length by using MEGAscript T7 Transcription Kit (ThermoFisher, Cat# AM1334), and purified with MEGAclear Transcription Clean-Up Kit (ThermoFisher ...
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bioRxiv - Plant Biology 2023Quote: ... The roots were lyophilized in vacuo and analyzed for total N and 15N content using elemental analysis–isotope ratio mass spectrometry (Flash EA1112-DELTA V PLUS ConFlo III system, Thermo Fisher Scientific).
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bioRxiv - Microbiology 2023Quote: ... Infectious titers were determined by focus-forming unit assay whereby foci of infection were measured using anti-nucleocapsid (N) antibody (Invitrogen; MA1-7403). Where indicated ...
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bioRxiv - Molecular Biology 2024Quote: ... level was measured from the plasma of representative subsets of the groups (n=16) using an automated analyzer (Indiko Plus, Thermo Fisher, US).
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bioRxiv - Biochemistry 2023Quote: ... Dried samples were redissolved with 300 μL of 0.1% TFA in H2O and further fractionated using high-pH reversed-phase peptide fractionation kits (ThermoFisher, P/N 84868) following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... the co-localization of bacterial biomass and necrotic tissue was assessed by a pathologist in samples with high bacterial biomass (n = 21) using an EVOS M7000 microscope (Thermofisher MA, USA).
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bioRxiv - Plant Biology 2023Quote: ... L−1 of 3’-biotin-labeled primer probe purified by high-pressure liquid chromatography (HPLC) for 4-12 h at 42°C in Hybaid Shake ‘n’ Stack (Thermo Fisher Scientific) with gentle rotation ...
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bioRxiv - Microbiology 2024Quote: ... Peptides were loaded on a trapping column and eluted over a 75μm x 50cm analytical column (Thermo Fisher P/N ES-803) at 300 nL/min using a 1hr reverse phase gradient ...
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bioRxiv - Cell Biology 2024Quote: ... at a flow rate of 0.3% µL/min and separated on a C18 analytical column (75 µm × 50 cm; PepMap RSLC C18; P/N ES803; Thermo Fisher Scientific). Peptides were then electrosprayed using 2.3 kV into the ion transfer tube (300° C ...
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bioRxiv - Cell Biology 2024Quote: ... Tryptic peptides (2 µg) were loaded onto a C18 trap (75 µm × 2 cm; Acclaim PepMap 100, P/N 164946; Thermo Fisher Scientific) at a flow rate of 2 µL/min of solvent A (0.1% formic acid and 3% acetonitrile in LC-mass spectrometry grade water) ...
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bioRxiv - Genomics 2024Quote: ... and once with 1X PBS for 5 min each before blocking with 100 mM N-succinimidyl (acetylthio) acetate (NHS-acetate, Thermo Fisher Scientific) in NHS-acetate buffer (0.1 M NaP ...
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bioRxiv - Microbiology 2024Quote: ... GFP images or RFU were collected at indicated time points post-infection by a fluorescence microscope (MI52-N) or a Varioskan LUX Multi-well Luminometer (Thermo Fisher Scientific).
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bioRxiv - Neuroscience 2024Quote: Striatum tissues from Cacna1dG407R/+ mice and wildtype siblings were collected and homogenized in N-Per Neuronal protein extraction buffer (Thermo Fisher Scientific) supplemented with Protease inhibitors (Halt Protease and Phosphatase Inhibitor Cocktail ...
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bioRxiv - Molecular Biology 2024Quote: ... 2.5 x 105 cells were in seeded in 6-well plate and after 24 h 1000 ng px330-BbsI-PITCh-UPF1-N and 500 ng donor plasmid pCRIS-PITChv2-PurR-FLAG were transfected using Lipofectamine 2000 (Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2024Quote: Total RNA was isolated from 5 dpf wild type and vps4a mutant larvae (n = 15-20 each genotype) using TRIzol reagent (Thermo Fisher, 15596026) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... and inserted with an N-terminal FLAG-tag via NheI and NotI restriction sites into the tetracycline-inducible pcDNA5/FRT/TO vector (Thermo Fisher Scientific). For stable integration ...