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Citations for Thermo Fisher :
2151 - 2200 of 10000+ citations for 6H Dibenzo b d pyran 3 pentanaminium 6a 7 10 10a tetrahydro 1 hydroxy N N N 6 6 9 hexamethyl 6aR trans 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: QuantStudio 6 PCR system (Life Technologies). Data were normalized with the maximal fluorescence intensity as 100%.
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bioRxiv - Neuroscience 2023Quote: ... 6-diamidino-2-phenylindole (DAPI, Invitrogen) nuclear stain ...
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bioRxiv - Immunology 2023Quote: ... IL-6 (Invitrogen, #88-7064-22), and IL-1β (Invitrogen ...
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bioRxiv - Immunology 2023Quote: ... IL-6 (Invitrogen, #88-7066-88), IL-8 (Invitrogen ...
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bioRxiv - Plant Biology 2023Quote: ... and QuantStudio 6 (Thermo Fisher Scientific). Gene-specific primer sets (Supplemental Table 3 ...
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bioRxiv - Neuroscience 2023Quote: ... Essential 6(tm) Medium (Thermo Fisher) was added to the culture for 2 days followed by addition of neural induction media (Advanced DMEM/F-12 (1:1) ...
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bioRxiv - Bioengineering 2023Quote: ... on a QuantStudio 6 (Applied Biosystems, QuantStudio Real-Time PCR software v.1.3) ...
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bioRxiv - Neuroscience 2023Quote: ... DAPI (4’,6-diamidino-phenylindole, Invitrogen) was added to the secondary antibody solution ...
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Short-range interactions between fibrocytes and CD8+ T cells in COPD bronchial inflammatory responsebioRxiv - Pathology 2023Quote: ... pH 6 (Fisher Scientific, Illkirch, France) at 96°C in a Pre-Treatment Module (Agilent ...
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bioRxiv - Neuroscience 2024Quote: ... in Essential 6 medium (Gibco, A1516401) supplemented with two SMAD pathway inhibitors – dorsomorphin (2.5 μM ...
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bioRxiv - Molecular Biology 2023Quote: ... The QuantStudio 6 system (ThermoFisher Scientific) was employed for running the qRT-PCR experiments ...
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bioRxiv - Neuroscience 2023Quote: ... coated 6-well plates (Nunc, Denmark).
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bioRxiv - Neuroscience 2023Quote: ... Hoescht 33342 (6 µg/mL; Invitrogen) was used to identify nuclei ...
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bioRxiv - Cancer Biology 2023Quote: ... anti-cytokeratin 5/6 (Invitrogen, MA191106) (1:100 ...
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bioRxiv - Genomics 2023Quote: ... and QuantStudio 6 FLEX machine (Thermofisher). Antibodies used for immunoprecipitation were ...
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bioRxiv - Genomics 2023Quote: ... and QuantStudio 6 FLEX machine (Thermofisher). Antibodies used for immunoprecipitation were ...
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bioRxiv - Developmental Biology 2024Quote: ... 6-diamidino-2-phenylindole (DAPI, Invitrogen). After washing slides were mounted in Elvanol ...
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bioRxiv - Cell Biology 2024Quote: ... IL-6 (Thermo Fisher Scientific, #P620), P16 (SCBT ...
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bioRxiv - Immunology 2024Quote: ... in 6-well plates (Thermo Scientific) at 1.5x106 cells per well for 1 h at 37°C in warm RPMI-1640 medium (GIBCO) ...
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bioRxiv - Cell Biology 2024Quote: ... 6) glucose-free DMEM (Gibco, 11966025) for 24 hr ...
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bioRxiv - Molecular Biology 2024Quote: ... and 6 mM magnesium chloride (Invitrogen) was added ...
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bioRxiv - Molecular Biology 2024Quote: ... and 6 mM magnesium chloride (Invitrogen) was assembled ...
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bioRxiv - Cell Biology 2020Quote: ... 1.8 mM CaCl2, 6 mM NaHCO3, 5.5 mM D-glucose, 25 mM HEPES, pH 7.4 supplemented with Gibco MEM Amino Acids and MEM Non-Essential Amino Acids solution ...
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bioRxiv - Cell Biology 2021Quote: ... on 12 or 15 mm coverslips in a 6-well plate and 10 nM siRNA-1 or -2 was transfected using RNAi MAX reagents (Invitrogen) according the manufacturer protocol ...
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bioRxiv - Cell Biology 2021Quote: ... They were washed in PBST for 6 × 10 min before being incubated with secondary antibody (1:400 in PBST) (ThermoFisher) for 30 min at room temperature ...
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bioRxiv - Immunology 2023Quote: ... One day prior to the co-culture 70- and 20-4_1BBζ cells were defrosted and cultured in a density of 1 × 10^6 cells/mL in RPMI containing1X GlutaMAX (Gibco), 1X P/S and 10% FCS supplemented with 10 ng/mL IL7 and IL15 overnight ...
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bioRxiv - Microbiology 2024Quote: ... blocked with 2% BSA in a PBS buffer for 10 min and incubated with 0.1–1 μg/ml DAPI (4’, 6-diamidyno-2-fenyloindol, Molecular Probes) and WGA-Texas Red (Wheat Germ Agglutinin-Texas Red ...
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bioRxiv - Neuroscience 2024Quote: ... 7-aminoactinomycin D (00-6993-50, Invitrogen, USA) was used for live-dead staining and analyzed on an Attune NxT acoustic focusing instrument (ThermoFisher Scientific ...
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bioRxiv - Genetics 2021Quote: ... using a Leica M205C stereomicroscope (190-240X magnification) and collected directly into sterile 0.2 mL PCR flat-cap tubes containing Prep-n-GoTM Lysis buffer (ThermoFisher Scientific, Carlsbad, CA, USA). This was done via a sterile tungsten needle and water-soluble 3MTM adhesive ...
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bioRxiv - Microbiology 2022Quote: ... and expression clones with N-terminal fusion tags were produced simply by Gateway cloning (Gateway™ LR Clonase™ II Enzyme mix, Invitrogen, 11791020). The Lenti-NSP2 was constructed by using the destination vector ...
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bioRxiv - Molecular Biology 2021Quote: ... cDNA was generated using 500 ng of total RNA and the High-Capacity cDNA with Reverse Transcription kit (Applied Biosystems p/n 4368814). QPCR was performed using the Wafergen SmartChip system (TakaraBio ...
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bioRxiv - Genomics 2019Quote: ... Samples were heat-denatured (95°C/ 5 min) and incubated o/n at 37°C with 150 U of λ-exonuclease (λ-exo; Thermo Scientific) in the presence of 40 U of RNAseOUT ...
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bioRxiv - Plant Biology 2020Quote: ... each slide was incubated overnight at 4°C with 100 μL of rabbit polyclonal anti-β-glucuronidase antibody (N-Terminal, 5420, Molecular Probes, Invitrogen) diluted 1:200 in fresh blocking buffer (3% BSA in 1X PBS) ...
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O-GlcNAcylation reduces phase separation and aggregation of the EWS N-terminal low complexity regionbioRxiv - Biochemistry 2021Quote: ... The LCRN coding sequence was inserted in frame with a N-terminal hexahistidine (His6)-SUMO tag in a Champion pET SUMO expression vector (Invitrogen, Carlsbad, CA, USA) by Gibson Assembly (New England Biolabs ...
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bioRxiv - Microbiology 2021Quote: ... we designed construct PolI-SARS-CoV2-NLuc-N-HDR and ordered this as insert in the pMK-RQ backbone (Invitrogen construct ID 20ACX40P). The resulting plasmid pPolI-SARS-CoV2-NLuc-N was digested with XbaI prior to transfection to eliminate background (read-through ...
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bioRxiv - Neuroscience 2019Quote: ... HPLC-purified dipeptide repeats were purchased from Pepscan and were either labelled with a single Cy5 dye at the N-terminus during synthesis or were labelled non-site-specifically in-house using Alexa488-Fluor® Protein Labeling Kit (Thermo Fisher Scientific) using published methods (94).
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bioRxiv - Plant Biology 2020Quote: The coding DNA sequence for human Factor IX without the native signal peptide was synthesized as N-terminal translational fusion to the signal-peptide coding sequence of PpAP1 (Pp3c5_19520V3.1)42 by GeneArt (Thermo Fisher Scientific, Waltham, MA, USA). It was cloned into the pAct5-MFHR1 plasmid41 to generate the final FIX expression vector ...
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bioRxiv - Immunology 2021Quote: ... A tPA signal was added at the N-terminus to facilitate protein secretion and the construct was cloned into pcDNA3.4 vector (Thermo Fisher, Waltham, MA, USA) which drives transcription via a CMV promoter ...
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bioRxiv - Biochemistry 2022Quote: ... was cloned into the pFastBac1 vector with a 10xHis tag appended at the N terminus and was expressed in Sf9 cells (Gibco, catalog number 12659017). The cells were cultured at 27 °C for 60 h after being infected with baculoviruses using the Bac-to-Bac system (Life Technologies) ...
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bioRxiv - Microbiology 2022Quote: SARS-CoV-2 N and goblet cells were co-stained using rabbit anti-SARS-CoV-2 Nucleocapsid monoclonal antibody (HL453) (Invitrogen, cat# MA5-36272) (1:100 dilution ...
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bioRxiv - Microbiology 2022Quote: ... Plasmids were targeted in a second bioreactor sampling experiment (n=2 for both bioreactors) for which the Plasmid Miniprep kit was used according to manufacturer’s instruction (Thermo Fisher Scientific, Waltham, US), with the addition of a step homogenizing the cells before processing ...
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bioRxiv - Cell Biology 2019Quote: ... This exogenous N-terminal GFP fusion was made by first cloning TtPOC5 into the pENTR4 Dual Selection Entry Vector (Invitrogen, Carlsbad, CA, USA) and then using the Gateway cloning system (Invitrogen ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were then incubated at 4°C overnight with the primary antibodies (Mouse anti-Dyrk1a: Abnova, Cat. N. H00001859-M01; Rabbit anti-Camk2a: Molecular probes, PA5-14315). After washing ...
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bioRxiv - Neuroscience 2021Quote: ... GEMs were broken and the single strand cDNA was cleaned up using DynaBeads® MyOne™ Silane Beads (Life Technologies, P/N 37002D). cDNA was amplified using a Bio-Rad PTC-200 Thermal cycler with 0.2ml 8-strip non-Flex PCR tubes ...
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bioRxiv - Immunology 2022Quote: ... and the N-terminal peptidase domain of human ACE2 were expressed using the Bac-to-Bac Baculovirus Expression System (Invitrogen, Carlsbad, CA, USA) and purified by Strep-Tactin Sepharose (IBA Lifesciences ...
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bioRxiv - Cancer Biology 2022Quote: ... cells (n = 500 000 cells/well) were transfected with 25 nM of each siRNA individually using Lipofectamine® 3000 Transfection Reagent (ThermoFisher Scientific, # L3000075) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2020Quote: ... The distillates from the KCl extracts were quantified by titration and then acidified and oven-dried at 60 °C for N isotope analysis by mass spectrometry (Finnigan MAT251, Thermo Fisher, Waltham, MA) according to Hauck et al ...
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bioRxiv - Molecular Biology 2020Quote: ... The reaction was incubated 2h at 37°C and then loaded on a 15% acrylamide/8M urea precast gel (Invitrogen, cat n° EC6885BOX). The ligated RNA was purified through gel extraction ...
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bioRxiv - Molecular Biology 2020Quote: ... The second strand cDNA was generated by performing one PCR cycle using Platinum II Hot start Taq Polymerase (Thermo Fisher, cat n°14966001). The reaction included 20 μLsingle-strand cDNA ...
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bioRxiv - Biochemistry 2020Quote: ... cDNA was generated using 500 ng of TotalRNA and Applied Biosystems High Capacity cDNA with Reverse Transcription kit (Applied Biosystems p/n 4368814). cDNA ...