Labshake search
Citations for Thermo Fisher :
2201 - 2250 of 10000+ citations for 6H Dibenzo b d pyran 3 pentanaminium 6a 7 10 10a tetrahydro 1 hydroxy N N N 6 6 9 hexamethyl 6aR trans 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biochemistry 2021Quote: ... NiV RdRp P/L complex was purified using Ni-NTA affinity chromatography based on the P protein N-terminal eight-histidine tag according to the manufacturer’s specifications (Thermo Scientific, Rockford, IL, USA). The protein identities of the purified HCV nsp5b RdRp and NiV RdRp P/L complex were confirmed by mass spectrometry (MS ...
-
bioRxiv - Cell Biology 2021Quote: ... Membranes were then cross-linked at 120J/m2 and then labelled RNA was detected using the Chemiluminescent Nucleic Acid Detection module kit (Thermofisher, cat n°: 89880) according to the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2020Quote: Chemical probes used in this work include alkDADA (Liechti et al., 2014), N-AlkTMM and O-AlkTMM (Foley et al., 2016) and FM4-64 FX (Invitrogen, Carlsbad, CA USA). AlkDADA was synthesized by the Einstein Chemical Biology Core and the TMM probes were kind gifts of Dr ...
-
bioRxiv - Biophysics 2022Quote: ... The terminal free amine on the spacer was reacted with the N-hydroxysuccinimide (NHS) ester group of sulfo-SMCC (Thermo Fisher Scientific, 22622). The maleimide group of sulfo-SMCC was subsequently conjugated to 3’ thiol modified DNA oligonucleotides as described19 ...
-
bioRxiv - Molecular Biology 2024Quote: ... 2008) N-terminally tagged with the V5 epitope was synthesized as a DNA string (GeneArt Strings DNA Fragments, Thermo Fisher Scientific, Schwerte, Germany) and cloned into pcDNA3.1 to obtain pcDNA3.1-V5-RHEB-Q64L ...
-
bioRxiv - Biochemistry 2024Quote: ... or 5 × 103 cells/mL (N. gruberi) in 100 µL of the indicated medium in white TC-treated 96-well plates (Thermo Scientific Nunc Microwell 136101). For assays under standard growth conditions (“+ glc”) ...
-
bioRxiv - Neuroscience 2024Quote: ... Neuronal differentiation was induced by culturing NGN2-NPC in neural differentiation medium (DMEM/F-12 + Glutamax, Gibco®; 1x N-2 supplement, Gibco®; 1x B27 supplement without vitamin A, Gibco®) supplemented with doxycycline (2µg/ml) ...
-
Development of monoclonal antibody-based blocking ELISA for detecting SARS-CoV-2 exposure in animalsbioRxiv - Microbiology 2023Quote: MARC-145 cells transfected with the recombinant pCAGGS-N plasmid were lysed in Pierce™ IP Lysis Buffer (Thermo Fisher Scientific, Waltham, MA), and then mixed with each of the purified anti-N mAbs ...
-
bioRxiv - Immunology 2023Quote: Open-reading frames coding for either Nefmut alone or fused with SARS-CoV-2 N protein were cloned into pVAX1 plasmid (Thermo Fisher, Waltham, MA) as previously described [20] ...
-
bioRxiv - Biochemistry 2023Quote: ... n=4) collected in microcentrifuge tubes were subjected to a 30-min incubation in 30 μM iodoacetamide (IAM) (Thermo Fisher Scientific, Waltham, MA) or N-ethylmaleimide (NEM ...
-
bioRxiv - Immunology 2023Quote: ... The peptides were separated at a flow rate of 300 nL/min on C18 pre-column (Acclaim PepMap™ 100, 75 µm × 2 cm, nanoViper, P/N 164946, ThermoFisher Scientific Incorporation) followed by analytical column (Acclaim PepMap™ RSLC C18 ...
-
bioRxiv - Biochemistry 2022Quote: ... The peptides were separated at a flow rate of 300 nL/minute on C18 pre-column (Acclaim PepMap™ 100, 75 μm□×□2 cm, nanoViper, P/N 164946, ThermoFisher Scientific Incorporation) followed by analytical column (Acclaim PepMap™ RSLC C18 ...
-
bioRxiv - Molecular Biology 2023Quote: Total protein from muscle tissue samples (n = 6 for EarlyMT and n = 16 for LateMT) was extracted using an extraction buffer containing Tissue extraction Reagent I (FNN0071, Invitrogen, Waltham, Massachusetts, USA), HALT Protease and Phosphatase Inhibitor Cocktail (1:100 ...
-
bioRxiv - Biochemistry 2023Quote: ... Both pDEST-CMV-N-EGFP-wtG3BP2 and pDEST-CMV-N-EGFP-C73SG3BP2 plasmids were amplified and extracted using PureLink™ HiPure Plasmid Maxiprep Kit (Invitrogen™, K210007) before use.
-
bioRxiv - Pharmacology and Toxicology 2024Quote: NHR2 labeling was performed with the reactive Alexa Fluor® 488 dye using N-hydroxysuccinimide (NHS)–ester chemistry (NHS Ester Protein Labeling kit, ThermoFisher Scientific, USA), which reacts efficiently with primary amines of proteins to form stable dye– protein conjugates ...
-
bioRxiv - Cell Biology 2024Quote: ... GEMs were then broken and the single-stranded cDNAs were cleaned up with DynaBeads MyOne Silane Beads (Thermo Fisher Scientific; P/N 37002D). The cDNAs were PCR amplified ...
-
bioRxiv - Developmental Biology 2024Quote: ... Dissociated cells were blocked in 6% BSA and immunolabeled with anti-α3 antibody (mouse Anti-ATP1A3 xVIF 9-G10, MA3-915, Invitrogen, Thermo Fisher scientific) for 2 hours 200 times in PBS1x containing 1%BSA or at room temperature ...
-
bioRxiv - Neuroscience 2022Quote: ... and medium from day 3-7 is Neurobasal Plus medium (NB-Plus) supplemented with B-27 μg/mL (Thermo Fisher Scientific), GlutaMax 10 μg/mL (Thermo Fisher Scientific) ...
-
bioRxiv - Developmental Biology 2022Quote: Total RNA was isolated from 6 pairs of P21 mouse testes (3 pairs of wild type and 3 pairs of Mov10-/-) using TRIzol reagents (Thermo Fisher Scientific). 1 μg of total RNA from each sample was used to generate RNA-seq libraries using TruSeq Stranded mRNA Library Preparation Kit Set A (Cat ...
-
bioRxiv - Plant Biology 2021Quote: ... The liquid syringe was washed 6 times with each of 10 µL of 1:1 (v/v) acetone:hexane and HPLC grade methanol (Fisher Scientific Co, Edmonton Alberta) both before and after the liquid injection.
-
bioRxiv - Immunology 2023Quote: ... Raji B cells were labelled in serum-free medium with 10 μM 7-amino-4-chloromethylcoumarin (CMAC, Thermofisher, USA) for 1 hour at 37°C ...
-
bioRxiv - Molecular Biology 2024Quote: ... Caspase-3/7 was quantified using the Apo-ONE® Homogeneous Caspase-3/7 Assay (Promega, Fisher scientific) according to the manufacturer’s protocol ...
-
bioRxiv - Synthetic Biology 2020Quote: ... Cells were subcultured at a 1:5 to 1:10 ratio every 2–3 d using Trypsin-EDTA (Gibco #25300-054). The HEK293FT cell line tested negative for mycoplasma with the MycoAlert Mycoplasma Detection Kit (Lonza #LT07-318).
-
bioRxiv - Microbiology 2019Quote: ... 1 µL of 1 mg/mL DAPI (4′,6-diamidino-2-phenylindole; ThermoFisher Scientific) was added to each sample and incubated at RT in darkness for a further 5 min ...
-
bioRxiv - Developmental Biology 2019Quote: Total RNA from whole retinas was extracted using TRIzol (6 retinas from 3 fish per sample) (Invitrogen). RNA was quantified using Nanodrop spectrophotometer (Thermo Fisher Scientific) ...
-
bioRxiv - Microbiology 2021Quote: ... Reverse 5’-CGA AGG TGT GAC TTC CATG-3’) on a QuantStudio 6 Flex thermocycler (Applied Biosystems). A standard curve was established in parallel using purified SARS-CoV-2 viral RNA.
-
bioRxiv - Immunology 2020Quote: ... plates were incubated with 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) substrate (ABTS, Thermo Fisher Scientific) for 15 min at RT shielded from light and absorbance was measured at optical density (OD ...
-
bioRxiv - Molecular Biology 2023Quote: U2OS 2-6-3 cells (Shanbhag et al., 2010) were cultured in McCoy’s 5A (Modified) Medium (Gibco) supplemented with 10% fetal bovine serum (FBS ...
-
bioRxiv - Cell Biology 2023Quote: ... DAPI (4’,6-Diamidino-2-henylindole, dihydrochloride) was obtained from Invitrogen (Catalog: D1306, CAS:28718-90-3).
-
bioRxiv - Neuroscience 2023Quote: ... 6 and 8 weeks of maturation were loaded with 2.5 µM Fluo-3-AM (Molecular Probes, #F1242) dissolved in differentiation medium for 30 min at 37 °C and at 5% CO2 ...
-
bioRxiv - Cell Biology 2019Quote: Analysis was performed using the Applied Biosystems validated TaqMan primer-probe set (Hs00208333/ spans exon 6 and 7) and the ABI PRISM SDS7000 sequence detection system (Applied Biosystems). The ΔCT method was used to quantify relative mRNA levels as described in ABI user guide ...
-
bioRxiv - Immunology 2021Quote: ... FAM-ACACTAGCCATCCTTACTGCGCTTCG-BHQ) and PCR reactions were carried out on a QuantStudio 6 and 7 Flex Real-Time PCR System (Applied Biosystems). To generate a standard curve ...
-
bioRxiv - Biochemistry 2021Quote: ... The grids were blotted for 6∼7 s at 100 % humidity and plunge-frozen in liquid ethane using a Vitrobot Mark IV instrument (Thermo Fisher). The RBD/Legobody complex at 2.5mg/ml were incubated with MS(PEG)12 methyl-PEG-NHS-ester (Thermo Fisher ...
-
bioRxiv - Immunology 2021Quote: ... Reactions were carried out in duplicate for samples and standards on the QuantStudio 6 and 7 Flex Real-Time PCR Systems (Applied Biosystems) with the following thermal cycling conditions ...
-
bioRxiv - Microbiology 2022Quote: ... Reactions were carried out in duplicate for samples and standards on the QuantStudio 6 and 7 Flex Real-Time PCR Systems (Applied Biosystems) with the thermal cycling conditions ...
-
bioRxiv - Cancer Biology 2020Quote: ... Data analysis was performed with the QuantStudio 6 and 7 Flex Real-Time PCR System Software v1.0 (Applied Biosystems, Life Technologies) using the ΔΔCt method.
-
bioRxiv - Cancer Biology 2020Quote: ... Data analysis was performed with the QuantStudio 6 and 7 Flex Real-Time PCR System Software v1.0 (Applied Biosystems, Life Technologies) using the ΔΔCt method.
-
bioRxiv - Microbiology 2022Quote: ... Reactions were carried out in duplicate for samples and standards on the QuantStudio 6 and 7 Flex Real-Time PCR Systems (Applied Biosystems). The following thermal cycling conditions were used ...
-
bioRxiv - Microbiology 2021Quote: ... CAS number: 328–42–7), 2-Ketoglutaric acid, disodium salt, dehydrate (>99%, CAS number: 305–72–6) were purchased from Acros Organics, Belgium ...
-
bioRxiv - Microbiology 2021Quote: ... Reactions were carried out in duplicate for samples and standards on the QuantStudio 6 and 7 Flex Real-Time PCR Systems (Applied Biosystems) with the thermal cycling conditions ...
-
NVX-CoV2373 vaccine protects cynomolgus macaque upper and lower airways against SARS-CoV-2 challengebioRxiv - Immunology 2020Quote: ... Reactions were carried out on a Quant Studio 6 and 7 Flex Real-Time PCR System (Applied Biosystems, Foster City, CA) according to the manufacturer’s specifications ...
-
bioRxiv - Immunology 2021Quote: ... Reactions were carried out in duplicate for samples and standards on the QuantStudio 6 and 7 Flex Real-Time PCR Systems (Applied Biosystems) with the thermal cycling conditions ...
-
bioRxiv - Microbiology 2020Quote: COS-7 cells were seeded onto 6-well plates 24 hours prior to the transient transfection using Lipofectamine 2000 reagent (Thermo Scientific) according to the manufacturer’s protocol ...
-
bioRxiv - Cell Biology 2024Quote: ... the conversion of non-fluorescent 2’,7’-bis-(2- carboxyethyl)-5-(and-6)-carboxyfluorescein acetoxymethyl ester (BCECF AM) (Invitrogen, Waltham, MA) into a pH sensitive fluorescent indicator by the intracellular esterase was used to measure the pH ...
-
bioRxiv - Neuroscience 2023Quote: ... at 1,000g for 2 min and then immediately loaded into the QuantStudio 6 and 7 Flex real-time PCR system (ThermoFisher Scientific). A two-step cycling protocol was implemented to collect cycle threshold (Ct ...
-
bioRxiv - Cancer Biology 2020Quote: ... CellEvent Caspase-3/7 Detection Reagent (Invitrogen) and Hoescht were added to the media at concentrations listed in S4 Table and incubated for 30min at 37°C in 5% CO2 ...
-
bioRxiv - Cell Biology 2020Quote: ... CellEvent Caspase-3/7 (Life Technologies C10723), Fis1 polyclonal antibody (Proteintech 109561-AP) ...
-
bioRxiv - Cell Biology 2024Quote: ... and caspase 3/7 (Thermo Fisher Scientific) were prepared following the manufacturer instructions ...
-
bioRxiv - Cell Biology 2023Quote: ... Caspase 3/7 (Thermo Fisher Scientific, #C10423) activation and propidium iodide (Thermo Fisher Scientific ...
-
bioRxiv - Neuroscience 2021Quote: ... while levels of IL-6 in supernatants were determined using IL-6 mouse ELISA kit (Thermo Scientific). Similarly ...