Labshake search
Citations for Thermo Fisher :
51 - 100 of 10000+ citations for Canine Parvovirus 2 VP2 Capsid Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2023Quote: ... Stock virus was sub-passaged through Madin-Darby canine kidney (MDCK) cells in Dulbecco’s modified Eagle’s medium (DMEM; Gibco), harvested as tissue culture supernatant and viral titres determined by cytopathic effects on MDCK cells and expressed as the mean log10 tissue culture infective dose required to kill 50% of the cells (TCID50 ...
-
bioRxiv - Microbiology 2020Quote: ... Primers targeting the three capsid genes were designed based on these bracketing conserved regions and ordered from Invitrogen (Guangzhou).
-
bioRxiv - Microbiology 2024Quote: ... pDEST-FLAG-HA-nsP4 and pDEST-FLAG-HA-capsid vectors were obtained by recombination using Gateway LR clonase (Invitrogen) in the pDEST-FLAG-HA vector according to the manufacturer instructions ...
-
bioRxiv - Microbiology 2023Quote: ... cells were fixed and processed for immunostaining with mouse anti-VP2 (RT16.7) and secondary goat Alexa Fluor 488-conjugated anti-mouse antibodies (Thermo Fisher Scientific, cat. no. A-11029) as described2,71 ...
-
bioRxiv - Cell Biology 2020Quote: Anti-MAP2 antibody (microtubule associated protein 2; Thermofisher) was used to identify neurons ...
-
bioRxiv - Molecular Biology 2023Quote: ... SARS-CoV-2 N protein (MA1-7404, Invitrogen), TLR3 (ab62566 ...
-
bioRxiv - Cell Biology 2024Quote: The Microtubule Associated Protein 2 (MAP2) (Thermo Fisher Scientific cat ...
-
bioRxiv - Biochemistry 2020Quote: ... and Madin-Darby canine kidney (MDCK) cells were cultured in cell culture medium: Dulbecco’s modified Eagle medium (DMEM) Glutamax (Gibco) supplemented with 10% Fetal Bovine Serum (FBS ...
-
bioRxiv - Bioengineering 2021Quote: Human HEK293T and Madin-Darby Canine Kidney (MDCK) cells (ATCC, CRL-2936) were maintained in Dulbecco’s Minimal Essential Medium (DMEM) (Gibco) supplemented with 10% fetal bovine serum (Gibco) ...
-
bioRxiv - Developmental Biology 2022Quote: ... and Madin-Darby Canine Kidney (MDCK) cells (ATCC, CRL-2936) were maintained in Dulbecco’s Minimal Essential Medium (DMEM) (Gibco) supplemented with 10% fetal bovine serum (Gibco) ...
-
bioRxiv - Microbiology 2020Quote: ... The Madin-Darby Canine Kidney II (MDCK-II) cell line was maintained in Eagle’s Minimum Essential Medium (EMEM, Gibco), supplemented with 5% heat-inactivated FBS (Thermo Fisher Scientific) ...
-
bioRxiv - Microbiology 2021Quote: Vero cells that stably express the canine receptor CD150 (Vero-cCD150) were grown in advanced Dulbecco’s modified Eagle medium (DMEM; Gibco) supplemented with 10% (V/V ...
-
bioRxiv - Microbiology 2023Quote: ... Cells were washed in PBS and incubated in primary antibody solution containing astrovirus capsid monoclonal antibody 8e7 (Invitrogen MA5-16293) at 1:100 in 1% NGS/PBS for 1 hour at room temperature ...
-
bioRxiv - Microbiology 2022Quote: ... the number of capsid copies in the cDNA was quantified via qPCR using PowerUp SYBR Green Master Mix (ThermoFisher Scientific) in a 10 μL total reaction ...
-
bioRxiv - Biochemistry 2021Quote: ... Individual reactions contained 2 μM protein and 5X SYPRO Orange Protein Gel Stain (Invitrogen). DP7/maltoheptaose (Elicityl) ...
-
bioRxiv - Pathology 2019Quote: ... and canine IAV was extracted using 1 ml of culture supernatant using Trizol® LS Reagent (ThermoFisher Scientific, Waltham, MA) following manufacturer’s protocol ...
-
bioRxiv - Immunology 2021Quote: Madin Darby Canine Kidney (MDCK) cell were grown in Dulbecco modified Eagle medium (DMEM) containing 10% fetal bovine serum (FBS) (Gibco), 2 mM L- and 100 U/mL penicillin-streptomycin (Thermo Fisher) ...
-
bioRxiv - Microbiology 2023Quote: Influenza A virus (IAV) strain A/WSN/33 [H1N1 subtype] was propagated in Madin-Darby Canine Kidney (MDCK) cells (ThermoFisher). Cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM ...
-
bioRxiv - Microbiology 2023Quote: ... Viruses were propagated in Madin-Darby canine kidney (MDCK) cells (IRR FR-926) in Minimal Essential Media [MEM (GIBCO™)] with 0.002μg tosyl phenylalanyl chloromethyl ketone (TPCK ...
-
bioRxiv - Microbiology 2020Quote: One milliliter of monomeric capsid (5 mg/ml) was dialyzed in SnakeSkin dialysis tubing (10K MWCO, Thermo Scientific, Waltham, MA, USA) using a buffer that was high in salt and contained a reducing agent (Buffer 1 ...
-
bioRxiv - Developmental Biology 2021Quote: ... 2 μl Dynabeads Protein G (Thermo Fisher Scientific, 10004D), and 400 μl dilution buffer (20 mM Tris-HCl pH 8.0 ...
-
bioRxiv - Molecular Biology 2020Quote: ... HA Tag antibody for SaCas9 protein (2-2.2.14, Thermofisher), rabbit anti-GFP (A11122 ...
-
bioRxiv - Microbiology 2022Quote: ... SARS-CoV-2 E protein was purchased from ThermoFisher Scientific (Catalog# RP-87682) ...
-
bioRxiv - Immunology 2023Quote: ... recombinant SARS-CoV-2 S protein (RP-87680, Invitrogen) or recombinant SARS-CoV-2 spike RBD protein (40592-V08H ...
-
bioRxiv - Microbiology 2023Quote: ... pre-stained protein markers (SeeBlue Plus 2, ThermoFisher Scientific) were included ...
-
bioRxiv - Neuroscience 2023Quote: ... Protein samples were loaded along with the SeeBlue Plus 2 protein marker (LC5925, Life Technologies). The gel was run at 60V for 20 mins ...
-
bioRxiv - Molecular Biology 2022Quote: ... Wells were coated with 1 μg Gfp for anti-GfpNB or 1 μg commercially available SARS-CoV-2 Spike-RBD-domain protein for SARS-CoV-2 nanobody-Cts1-fusion proteins (Invitrogen, Waltham Massachusetts, USA). Recombinant Gfp was produced in E ...
-
bioRxiv - Cancer Biology 2020Quote: ... Protein extracts were eluted in 2% SDS and protein concentration was measured using MicroBCA Protein Assay Kit (Thermo Fisher Scientific). Peptide isolations were digested by trypsin and SDS was removed employing Detergent Removal Spin Columns (Thermo Fisher Scientific) ...
-
bioRxiv - Molecular Biology 2022Quote: ... Madin-Darby Canine Kidney (MDCK) cells were maintained in the same conditions with 10% FBS (Gibco™, FBS; Cat no. #10270106). Influenza B/Brisbane/60/2008 virus was used in this study.
-
bioRxiv - Biophysics 2022Quote: Madin-Darby canine kidney cells (MDCK II) were used in all studies and maintained in high glucose DMEM (Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (Thermo Fisher Scientific ...
-
bioRxiv - Molecular Biology 2023Quote: Madin-Darby canine kidney (MDCK) type IIG cells were maintained in low glucose MEM (#41090093, Thermo Fisher Scientific, GibcoTM, Paisley, UK) supplemented with 1 % (vol/vol ...
-
bioRxiv - Microbiology 2023Quote: ... Madin–Darby canine kidney (MDCK) cells (ATCC CCL34TM) were cultured in minimum essential medium (MEM) containing 10% fetal bovine serum (GIBCO Laboratories). Cells were grown at 37°C in a humidified atmosphere with 5% CO2.
-
bioRxiv - Genetics 2023Quote: ... capsid plasmid (15 μg per dish) and cargo plasmid (9 μg per dish) were mixed with serum-free DMEM (Thermo Fisher Scientific) and polyethylenimine (PEI ...
-
bioRxiv - Cell Biology 2019Quote: ... followed by 2 hours incubation with Protein A dynabeads (Invitrogen). Immunoprecipitated material was sequentially washed with buffer containing low salt (150 mM NaCl) ...
-
bioRxiv - Neuroscience 2021Quote: ... Proteins were transferred to nitrocellulose membranes using iBlot 2 (Invitrogen) and immunoblotting was performed as previously described in the western blot section.
-
bioRxiv - Neuroscience 2019Quote: ... protein concentration was measured using Qubit 2 Fluorometer (Thermo Scientific).
-
bioRxiv - Microbiology 2024Quote: ... SARS-CoV-2 S1 protein primary antibody (Thermo Fisher Scientific) at 1µg/ml were added for 1 h at 37°C ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... were supplemented with SDS to a final concentration of 2% and the protein concentration determined by BCA protein assay (Pierce BCA Protein Assay Kit, Thermo Scientific). Filter-aided sample preparation (FASP ...
-
bioRxiv - Immunology 2022Quote: ... Constant concentration (2 µg 100µl/well) of recombinant SARS-CoV-2 spike protein (RP-87680, Invitrogen) was added to the wells ...
-
bioRxiv - Microbiology 2020Quote: ... and Madin-Darby canine kidney (MDCK) cells were all maintained in Dulbecco’s modified Eagle’s medium (DMEM; Thermo Fisher Scientific, Ottawa, ON, Canada) supplemented with 10% fetal bovine serum (FBS ...
-
bioRxiv - Immunology 2020Quote: ACE-2 protein (hsACE-2, residues S19 to D615) was transiently transfected into HEK293E cells (Thermo Fisher) with PEI MAX (Polysciences ...
-
bioRxiv - Biophysics 2023Quote: ... and Madin-Darby Canine Kidney II (MDCK-II) (ECACC, Cat. No. 00062107) were cultured in Dulbecco’s modified Eagle medium (DMEM 61965026, Thermo Fisher Scientific, Waltham, USA) supplemented with 10% fetal bovine serum (FBS 102701036 ...
-
bioRxiv - Cell Biology 2020Quote: ... Proteins were eluted in 2× NuPAGE LDS Sample Buffer (Life Technologies) by boiling at 95 °C for 10 min ...
-
bioRxiv - Molecular Biology 2021Quote: ... Mouse IFN-alpha 2 (Ifna2) recombinant protein was purchased from Affymetrix eBioscience (catalog #14-8312) ...
-
bioRxiv - Molecular Biology 2021Quote: ... pre-cleared for 2 h with Protein A Dynabeads (Invitrogen, 10001D), followed by overnight incubation with 5 µg of antibody (anti-SKIV2L Proteintech group ...
-
bioRxiv - Microbiology 2021Quote: ... or SARS-CoV-2 E protein (1-75 aa) (Thermo Fisher) in coating buffer [0.05 M sodium carbonate–sodium bicarbonate (pH 9.6)] ...
-
bioRxiv - Cell Biology 2020Quote: ... Fura-2 BZiPAR and Protein G magnetic beads were from Invitrogen - Thermo Fisher Scientific (Waltham ...
-
bioRxiv - Physiology 2021Quote: ... mouse-anti Microtubule-associated protein 2 (MAP2) (Invitrogen, Cat # 13-1500) (1:1000) ...
-
bioRxiv - Neuroscience 2020Quote: ... lysosome-associated membrane protein 2 (LAMP2; 1:1000; Thermofisher, Waltham, MA), or phopsho-ribosomal S6 protein (PS6 ...
-
bioRxiv - Neuroscience 2022Quote: ... Protein bands were revealed using ECL reagent (ECL 2, Thermo Scientific) with a Typhoon 9419 phosphorimager (GE Healthcare ...