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Citations for Thermo Fisher :
1901 - 1950 of 10000+ citations for SARS CoV 2 Spike Glycoprotein S1 Sheep Fc Tag HEK293 Biotin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Lactic Acid Containing Polymers Produced in Engineered Sinorhizobium meliloti and Pseudomonas putidabioRxiv - Microbiology 2019Quote: ... the gel was used for His-tag staining following the InVision(tm) His-tag In-Gel Stain protocol provided by Invitrogen.
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bioRxiv - Synthetic Biology 2019Quote: ... An anti-6His antibody (6x-His Tag Polyclonal Antibody) and an anti-HA antibody (HA Tag Polyclonal Antibody) were purchased from Invitrogen. Western blot analysis was conducted as described previously 45.
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bioRxiv - Biochemistry 2022Quote: ... with a C-terminal Strep tag for purification and a foldon tag for trimerization was inserted into the pFastBac-Dual vector (Invitrogen) and was expressed using Bac-to-Bac baculovirus system (Invitrogen) ...
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bioRxiv - Biochemistry 2022Quote: ... with a C-terminal Strep tag for purification and a foldon tag for trimerization was expressed in FreeStyle 293-F cells (Invitrogen). The plasmid was transiently transfected at a density of 2 × 106 cell per ml using polyethyleneimine (PEI ...
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bioRxiv - Microbiology 2022Quote: ... A recombinant SPATR (rSPATR) fused to a polyhistidine tag (His-tag) was produced in BL21-CodonPlus-RIL competent cells (Invitrogen) and protein purification was performed under denaturing conditions using Ni-NTA Agarose (Qiagen ...
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bioRxiv - Molecular Biology 2020Quote: ... ORFs were cloned in pDEST15 (containing the GST tag) and pDEST17 Gateway (containing the His6 tag) vectors (Thermo Fisher Scientific). For Y2H assays ...
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bioRxiv - Cancer Biology 2021Quote: ... Biotin labelling of the nucleosome assembly was conducted using the EZ-Link™ Psoralen-PEG3-Biotin kit (Thermo Scientific™) as described in the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2021Quote: ... cells were washed twice with ice-cold PBS and treated for 30min with 0.6 mg/ml of biotin in PBS (EZLink Sulfo-NHS-LC-Biotin, Thermo Scientific). Next ...
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bioRxiv - Neuroscience 2022Quote: ... the neurons were washed with cold PBS (all wash steps were carried out with PBS supplemented with 1 mM CaCl2 and 0.5 mM MgCl2) and incubated with 1 mg/ml biotin (EZ-Link™ Sulfo-NHS-Biotin, ThermoFisher), shaking ...
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bioRxiv - Molecular Biology 2020Quote: ... Biotin was attached to cross-linked RNA duplexes by incubating with 150 µM Click-IT Biotin sDIBO Alkyne (Life technologies) under constant agitation at 37 °C for 1.5 hours ...
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bioRxiv - Microbiology 2022Quote: ... Click-chemistry reaction for protein detection was performed using biotin alkyne (PEG4 carboxamide-propargyl biotin) according to manufacturer’s instructions using the Click-iT™ Protein Reaction Buffer Kit (Invitrogen). Labeled proteins were separated by SDS-PAGE ...
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bioRxiv - Biophysics 2022Quote: Biotinylated-BtuF* described in previous section was tethered to the surface of a quartz microfluidic flowcell that was pre-functionalized with polyethylene glycol (PEG)/biotin-PEG (mPEG-SVA MW3400 and Biotin-PEG-SVA MW5000, respectively, Laysan Bio) and pre-derivatized with streptavidin (ThermoFisher) via a streptavidin-biotin-streptavidin bridge52,53 ...
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bioRxiv - Cell Biology 2023Quote: ... 50 µl of freshly prepared solution of Biotin tracer (10 mg/ml EZ-Link Sulfo-NHS-LC-Biotin, Thermo Fisher Scientific ...
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bioRxiv - Immunology 2023Quote: ... The mole-to-mole ratio of biotin to protein was quantified using the Pierce Biotin Quantitation Kit (Thermo Fisher Scientific). Biotinylated Spike was conjugated to streptavidin-FITC (BioLegend ...
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Targeted degradation of PCNA outperforms stoichiometric inhibition to result in programed cell deathbioRxiv - Cell Biology 2022Quote: ... HEK293 and PLC/PRF/5 cells were cultured in MEM GlutaMAX™ (Gibco). All media were supplemented with 10% Fetal Bovine Serum (HyClone) ...
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bioRxiv - Cell Biology 2019Quote: ... Positively transfected cells (HEK293) were selected with hygromycin B (Cat.10687010, Thermo Fisher), seeded in 24-well plates at a density of 20,000 cells/well ...
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bioRxiv - Neuroscience 2019Quote: Human embryonic kidney (HEK293) cells were maintained in Dulbecco’s modified eagle medium (Gibco) containing 10% fetal bovine serum (HyClone) ...
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bioRxiv - Genetics 2019Quote: ... The HEK293 cells were cultured in Dulbecco’s modified Eagle’s medium (Life Technologies, Inc.) with 10% fetal bovine serum (Hyclone) ...
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bioRxiv - Genomics 2020Quote: HEK293 cells were cultured in Dulbecco’s modified Eagle’s medium (Invitrogen, Grand Island, NY) with 10% (v/v ...
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bioRxiv - Cell Biology 2021Quote: Hela and HEK293 cells were grown in Dulbecco’s Modified Eagle’s Medium (DMEM; Gibco) supplemented with 10% Fetal Bovine Serum (FBS ...
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bioRxiv - Neuroscience 2020Quote: Human embryonic kidney cells (HEK293) were cultured in Dulbecco’s modified Eagle’s media (Gibco) containing 10 % v/v fetal bovine serum (heat-inactivated ...
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bioRxiv - Cell Biology 2021Quote: HeLa cells and HEK293 Flp-In™ T-Rex cells (Thermo Fisher Scientific) were cultured in Dulbecco’s Modified Eagle Medium (DMEM ...
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bioRxiv - Molecular Biology 2021Quote: Suspension-adapted HEK293 cells (Freestyle™ 293-F cells, R790-07, Life Technologies) were grown as recommended in Freestyle™ 293 Expression Medium (12338026 ...
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bioRxiv - Biophysics 2021Quote: ... Suspension cultures of HEK293S GnTI− cells were grown in Freestyle 293 media (GIBCO) supplemented with 2% FBS (GIBCO ...
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bioRxiv - Microbiology 2020Quote: HEK293 and HEK293T were cultured in Dulbecco’s modified Eagle’s medium (DMEM, Gibco, USA) with 10% heat-inactivated fetal bovine serum (FBS ...
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bioRxiv - Biochemistry 2022Quote: ... plasmids were transfected into HEK293 cells in triplicate using Lipofectamine 2000 (Thermofisher Scientific) in the presence of various amounts of DHT ...
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bioRxiv - Developmental Biology 2022Quote: ... The recombinant plasmids were introduced into HEK293-EBNA cells (Invitrogen, Carlsbad, CA, USA) using FuGENE 6 transfection reagents (Roche ...
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bioRxiv - Developmental Biology 2022Quote: ... Recombinant zebrafish Nid2a and Hmcn1 fragments were generated in HEK293-EBNA cells (Invitrogen) and purified as described above for the mouse proteins and used to immunize rabbits or guinea pigs (Pineda Antikörper Service ...
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bioRxiv - Neuroscience 2019Quote: HEK293 cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM; Gibco 11995-065), that was supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Molecular Biology 2020Quote: 5 × 15cm plates of HEK293 Flp-In T-REx cells (ThermoFisher Scientific, R78007) at 80% confluency were harvested in 15mL DMEM media by trypsinization ...
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bioRxiv - Microbiology 2022Quote: ... Transfections of HEK293T or HEK293 cell suspensions were performed using Lipofectamine 2000 (Invitrogen) and Optimem (Invitrogen ...
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bioRxiv - Cell Biology 2022Quote: ... We cultured HEK293 cells in minimum essential medium (MEM, Invitrogen, Cat. No. 11095080) supplemented with 10% fetal bovine serum (Thermo Fisher ...
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bioRxiv - Bioengineering 2019Quote: ... plasmids were co-transfected into HEK293-T cells using Lipofectamine 2000 (Life Technologies). After 4 h ...
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bioRxiv - Biochemistry 2020Quote: ... Human 28S and 18S rRNAs were extracted from HEK293 cells with TRIzol (Invitrogen). Intact rRNAs were isolated by pipetting from a native agarose gel after running the rRNA into wells in the center of the gel ...
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bioRxiv - Biophysics 2021Quote: ... HEK293S GNTI- cells (RRID: CVCL_A785) were grown in suspension in Freestyle media (Gibco) supplemented with 2% FBS (VWR ...
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bioRxiv - Genetics 2021Quote: ... HEK293 WT cells were transfected with both sgRNA vectors using Lipofectamine 2000 (Invitrogen). 24-hours after transfection cells were subjected to Puromycin selection (2ug/mL ...
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bioRxiv - Neuroscience 2020Quote: HEK293 cells were grown in DMEM medium with fetal bovine serum (Life Technologies) and 1% penicillin/streptomycin (Life Technologies ...
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bioRxiv - Biochemistry 2021Quote: ... HEK293S GnTi- cells were cultured in Freestyle 293 expression medium (Thermo Fisher Scientific) at 37 °C with 5% CO2 ...
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bioRxiv - Biochemistry 2021Quote: ... The HEK293S-GnTi− cells were cultured in Freestyle 293 medium (Thermo Fisher Scientific) supplemented with 5% CO2 at 37 ° C ...
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bioRxiv - Molecular Biology 2021Quote: ... HEK293 and HCT116 cells were cultured in Dulbecco’s Modified Eagle Medium (Gibco, 11995065) with 10% FBS (VWR ...
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bioRxiv - Cell Biology 2020Quote: ... HeLa and HEK293 cells were cultured in Dulbecco’s Modified Eagle Medium (DMEM, Gibco) containing 5 - 10% FBS (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2020Quote: ... HEK293T and HEK293 cells were grown in Dulbecco’s Modified Eagle Medium (DMEM, Invitrogen) which was supplemented with 10% fetal bovine serum (Gibco ...
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bioRxiv - Neuroscience 2022Quote: HEK293 FT cells were transfected with the BioID2 constructs using Lipofectamine 2000 (Invitrogen) in Opti-MEM ...
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bioRxiv - Physiology 2022Quote: ... HEK293 cells were transfected with human TRPA1 expressing plasmid using Lipofectamine 2000 (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... HEK293 and MC38 cells were incubated in DMEM (Meilunbio) with 10% FBS (Gibco). THP1-dual cells were incubated in RPMI-1640 (Meilunbio ...
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bioRxiv - Molecular Biology 2022Quote: ... Plasmids (3ug per well) were transfected into HEK293 cells using lipofectamine 2000 (Invitrogen), according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... HEK293S MGAT−/− cells were maintained in a 1:1 ratio of Freestyle (ThermoFisher) and Ex-Cell (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2023Quote: HEK293S GnTI− cells were grown at 37°C in Freestyle 293 medium(Invitrogen) supplemented with 1.5% FBS ...
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bioRxiv - Cell Biology 2023Quote: Hela and HEK293 cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM, Gibco) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Cell Biology 2023Quote: ... the HEK293-derived cells Flp-InTM T-RexTM 293 Cell Line (ThermoFisher Scientific) were stably transfected to express HA-TDP-43 upon induction with tetracycline (1 μg/mL) ...