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Citations for Thermo Fisher :
1801 - 1850 of 10000+ citations for SARS CoV 2 Spike Glycoprotein S1 Sheep Fc Tag HEK293 Biotin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2023Quote: HEK293 cells (ATCC, CRL-1573, RRID:CVCL_0045) and 293FT cells (Invitrogen, R70007, RRID:CVCL_6911) were cultured in DMEM high glucose medium with sodium pyruvate and L-glutamine ...
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bioRxiv - Neuroscience 2023Quote: ... while HEK293 cells were cultured at 37°C in DMEM Medium (Gibco). Both media were supplemented with 10% heat-inactivated fetal bovine serum and 1% penicillin/streptomycin ...
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bioRxiv - Molecular Biology 2023Quote: HEK293 cells were maintained in DMEM/F-12 (Gibco, cat no. 11320033) supplemented with 1% Penicillin-streptomycin (Gibco ...
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bioRxiv - Biophysics 2023Quote: HEK293 cells were transfected with ICD constructs using Lipofectamine 2000 (Thermo Fisher) and were selected with G418 ...
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bioRxiv - Cancer Biology 2024Quote: ... HEK293 cells were seeded overnight and transfected with Lipofectamine 3000 (Thermo Fisher) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: HEK293-6E suspension cells were cultured in FreeStyle F17 expression medium (Gibco) supplemented with 4 mM L-glutamine (Gibco) ...
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bioRxiv - Biochemistry 2024Quote: HEK293 Freestyle suspension cells were cultured in HEK Freestyle Media (Invitrogen, 12338018) grown at 37° C in a humidified shaking platform incubator with 10% CO2 ...
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bioRxiv - Bioengineering 2024Quote: HEK293-F suspension cells were cultured in FreeStyle 293 Expression Medium (Gibco) in shake flasks at 135 rpm ...
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bioRxiv - Biochemistry 2024Quote: HEK293 cells (ATCC) were grown in Dulbecco’s Modified Eagle Medium (DMEM, Gibco) with 10% fetal bovine serum (FBS ...
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bioRxiv - Immunology 2021Quote: The SARS-CoV-2 receptor-binding domain (RBDwt) and the RBD mutant (RBDmut) (K417N, E484K, N501Y) were expressed using Expi293F cells (Invitrogen, ThermoFisher Scientific, MA, USA). The genes that encode SARS-CoV-2 RBDwt (residues Arg319-Phe541 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... and SARS-CoV2 nucleocapsid (Invitrogen, MA1-7404, Waltham, Massachusetts, USA) antibodies were used ...
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bioRxiv - Systems Biology 2019Quote: Recombinant protein antigens were first labeled with biotin using EZ-Link™ NHS-LC-Biotin reagent (Thermo Fisher, #21366) and then hybridized with the PETAL array ...
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bioRxiv - Molecular Biology 2021Quote: ... were used following biotin blocking of tissues samples using endogenous Biotin-blocking kit (Life Technologies Corporation/Thermo fisher, E21390). For Aza-peptide preparation ...
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bioRxiv - Molecular Biology 2021Quote: ... were used following biotin blocking of tissues samples using endogenous Biotin-blocking kit (Life Technologies Corporation/Thermo fisher, E21390). For Aza-peptide preparation ...
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bioRxiv - Molecular Biology 2021Quote: ... the biotin-labeled probes were detected in the membrane using a biotin luminescent detection kit (Thermo Fisher Scientific, USA). The EMSA experiment was performed three times.
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bioRxiv - Cell Biology 2020Quote: ... 130 ng of total RNA was labeled with biotin using the FlashTag Biotin HSR RNA Labeling Kit (Applied Biosystems) according to manufacturer’s procedure ...
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bioRxiv - Neuroscience 2022Quote: ... interference from endogenous biotin and/or streptavidin-binding activity was moreover reduced by Avidin/Biotin Blocking kit (Thermo Fisher). Tissue sections were incubated overnight in a humidified chamber with following primary antibodies ...
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bioRxiv - Plant Biology 2022Quote: ... was synthesized (Integrated DNA Technologies) and biotin-labeled (Pierce™ Biotin 3’ End DNA Labeling Kit, Thermo Fisher Scientific). The sequence used is as follows ...
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bioRxiv - Biophysics 2021Quote: ... and both HRP-conjugated anti-mouse IgG Fc and anti-human IgG Fc were purchased from Invitrogen™ (Waltham ...
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bioRxiv - Immunology 2023Quote: ... Fc receptors were blocked by incubating cells with 1:50 anti- mouse CD16/CD32 (Fc block, Invitrogen) for 10 minutes at 4°C ...
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bioRxiv - Biochemistry 2021Quote: ... 1% PenStrep and transfected with plasmids encoding for the corresponding S glycoprotein (24 µg/dish) using lipofectamine 2000 (Life Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2021Quote: Filaments extracted from gerbera were subjected to SDS-PAGE and then the Pierce™ Glycoprotein Staining Kit (ThermoFisher Scientific, USA) was used as directed ...
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bioRxiv - Molecular Biology 2022Quote: NiV prefusion glycoprotein and avi-tagged probes were expressed by transfection in 293 Freestyle (293F) cells (Thermo Fisher Scientific, MA) using Turbo293 transfection reagent (SPEED BioSystem ...
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bioRxiv - Biochemistry 2021Quote: ... an equivalent of 1 μg glycoprotein digest was loaded onto an Acclaim PepMap RSLC C18 column (Thermo Fisher Scientific, Lithuania) and separated at a flow rate of 300nL/min using a gradient of 5% to 40% solvent B (80% acetonitrile with 0.1% formic acid ...
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bioRxiv - Cell Biology 2021Quote: ... and 4 μg of plasmid VSV-G encoding G glycoprotein of vesicular stomatitis virus(VSVG) using Lipofectamine 3000 Reagent (Invitrogen). 12 h later ...
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bioRxiv - Microbiology 2021Quote: ... and glycol-proteins and the DNase/Proteinase K treated matrix samples with the Pro-Q Emerald 300 glycoprotein stain (ThermoFisher).
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bioRxiv - Plant Biology 2020Quote: ... Dynabeads M280 Sheep anti-mouse IgG or Dynabeads Protein G (Thermo Fisher Scientific) were used for immunoprecipitation ...
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bioRxiv - Immunology 2021Quote: ... donkey anti-sheep AF 594 and AF 647 were obtained from Molecular Probes. DAPI and Filipin complex from Streptomyces filipinensis were purchased from Sigma.
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bioRxiv - Genomics 2021Quote: ... pre-bound to M-280 sheep anti-rabbit Dynabeads (Thermo Fisher Scientific, # 11204D). DNA was purified with phenol/chloroform/isoamyl alcohol and precipitated with ethanol ...
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bioRxiv - Cell Biology 2022Quote: ... donkey anti-sheep AF488 (1:1000 dilution, catalog no. A11015, Thermo Fisher Scientific).
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bioRxiv - Microbiology 2022Quote: ... 100 µL of 1% (v/v) sheep red blood cells (RBC) (Fisher Scientific) in PBS was combined with 100 µL of PBS ...
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bioRxiv - Neuroscience 2021Quote: ... Magnetic beads with coupled sheep antibodies anti-rabbit IgG (Dynabeads® M280; Invitrogen) were washed with 0.5% bovine serum albumin immonuglobulin-free (BSA Ig-free ...
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bioRxiv - Immunology 2020Quote: ... CD4 - and CD8-specific antibodies and subsequently sheep-anti-rat dynabeads (Invitrogen #11035). A second round of selection using a FACSAria Fusion cell sorter was performed on LIN-thymocytes to obtain DN CD44-thymocytes (DN3+DN4 populations ...
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bioRxiv - Biochemistry 2021Quote: ... inside Columbia agar supplemented with 4% of defibrinated sheep blood (Thermo Scientific Oxoid) with 0.5 µg/ml erythromycin and 0 or 0.1 mM IPTG ...
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bioRxiv - Neuroscience 2019Quote: ... Alexa Fluor 488 donkey anti-mouse (rabbit or sheep) IgG (1:1000; Invitrogen). F-actin was detected using Alexa Fluor™ 568 Phalloidin (1:300 ...
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bioRxiv - Molecular Biology 2021Quote: ... was pre-incubated with Dynabeads M-280 Sheep Anti-Rabbit IgG (Invitrogen, #11204D) for 1 hour and added to the lysates for immunoprecipitation at 4°C for 16 hours ...
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bioRxiv - Molecular Biology 2021Quote: ... Single cell suspension were incubated with sheep anti-rat IgG dynabeads (Invitrogen, 11035) coated with CD31 antibody (BD Pharmingen ...
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bioRxiv - Immunology 2022Quote: ... and incubated with 250 μl sheep anti-rat IgG Dynabeads (Thermo Fisher Scientific) for 30 min at 4°C with tilted rotation ...
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bioRxiv - Molecular Biology 2022Quote: ... followed by incubation with Alexa Fluor 568 donkey anti-sheep IgG (Thermo Fisher) diluted 1:500 ...
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bioRxiv - Molecular Biology 2022Quote: ... pre-equilibrated Dynabeads M-280 sheep anti-mouse IgG (Thermo Fisher Scientific, 11201D) was added to the reaction mixture and further incubated for 4 h at 4ºC with gentle rotation ...
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bioRxiv - Bioengineering 2023Quote: ... Sheep periosteum derived cells (sPDCs) were harvested with TrypLE Express (Life Technologies, UK) and seeded at 300 000 cells per 2 mL chondrogenic medium resulting in a microtissue size of 250 or 1000 cells for aggrewell400 and aggrewell800 respectively ...
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bioRxiv - Cancer Biology 2023Quote: ... and using Dynabeads™ M-280 Sheep Anti-Rabbit IgG (Invitrogen Cat# 11203D). Chromatin Immunoprecipated DNA was amplified by qPCR using a CFX Connect Real-Time PCR Detection System (Bio-Rad ...
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bioRxiv - Microbiology 2023Quote: ... and grown on TSA with 5% sheep’s blood (Fisher Scientific, #221261, Hampton, NH) to determine the number of microbes added to the ALI cultures.
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bioRxiv - Microbiology 2023Quote: ... and plated on TSA with 5% sheep’s blood (Fisher Scientific, #221261, Hampton, NH) or 1X TSB (Becton ...
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bioRxiv - Immunology 2023Quote: ... Lin+ cells were depleted using sheep anti-rat IgG immunomagnetic beads (Dynabeads, Invitrogen). 8-10 x 106 Lin-depleted BM cells from CD45.1 congenic mice were retro-orbitally injected into 6-8-week-old lethally irradiated (1200 rads ...
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bioRxiv - Molecular Biology 2024Quote: ... 25 µl of Dynabeads M-280 Sheep Anti-Mouse IgG (Thermo Fisher Scientific) was incubated with 2 mg of anti-BrdU antibody (BD Biosciences ...
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bioRxiv - Molecular Biology 2024Quote: ... 25 µl of Dynabeads M-280 Sheep Anti-Mouse IgG (Thermo Fisher Scientific) was incubated with 2 mg of anti-BrdU antibody (BD Biosciences ...
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bioRxiv - Biochemistry 2020Quote: ... Each sample was labeled for 2 h at 22°C with a different TMT-tag obtained from a TMT10plex kit (Thermo Fisher Scientific), and then quenched (0.27% hydroxylamine ...
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bioRxiv - Immunology 2021Quote: ... NC_045512.2 with N-terminal signal peptide and C-terminal thrombin cleavage site-TwinStrep-8xHis-tag) were expressed in Expi293F (Thermo Fisher Scientific) cells at 37°C and 8% CO2 ...
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bioRxiv - Immunology 2020Quote: ... were coated overnight at 4°C with 2 ug/ml of mouse anti-His-tag antibody (Invitrogen cat. #MA1-21315-1MG, Thermo Fisher Scientific) in PBS ...