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Citations for Thermo Fisher :
1901 - 1950 of 10000+ citations for Recombinant Human FCGRT & B2M Protein His Avi Strep II tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2021Quote: ... and re-suspended at a density of 104 cells/mL in pre-warmed complete minimum essential media (MEM) that contains 5 mg/mL human recombinant insulin (Gibco), 1X MEM non-essential amino acids (NEAA ...
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bioRxiv - Cancer Biology 2020Quote: ... HFKs and iHFKs were grown and maintained in keratinocyte-serum-free media (KSFM) supplemented with human recombinant epidermal growth factor and bovine pituitary extract (Invitrogen). IMR-90s normal human diploid lung fibroblasts were purchased from ATCC and maintained in Dulbecco’s Modified Eagle Medium (DMEM ...
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bioRxiv - Neuroscience 2020Quote: ... recombinant human epidermal growth factor (5 ng/ml) and additionally supplemented with freshly added recombinant human fibroblast growth factor-basic (1 ng/ml; Gibco). hCMEC/D3 cells used for the experiments were between passages 6 and 11 ...
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Dynamic chromatin organization and regulatory interactions in human endothelial cell differentiationbioRxiv - Developmental Biology 2022Quote: ... from the RUES2 line (RUESe002-A; WiCell) were maintained on recombinant human Laminin-521 matrix (rhLaminin521; Biolamina) in Essential 8 (E8) media (ThermoFisher) at a density of 0.5 µg/cm2 ...
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bioRxiv - Genomics 2019Quote: ... hiPSCs were cultured on plates pre-coated with 0.5 μg/cm2 recombinant human Laminin-521 matrix (rhLaminin521; Biolamina) with daily changes of antibiotic-free Essential 8 (E8) media (ThermoFisher). Cells were passaged as small clumps with Versene (ThermoFisher) ...
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bioRxiv - Biochemistry 2022Quote: ... and recombinant human Interleukin-2 (rhIL-2, 100 units/mL)) in the presence of anti-CD3/CD28 Dynabeads (Fisher Scientific) at a bead:cell ratio of 1:1 ...
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bioRxiv - Bioengineering 2022Quote: The human embryonic stem cell line H9 (WiCell, authenticated at source) was grown on recombinant human vitronectin (Thermo) coated cell culture dishes in StemFlex Medium (Gibco). Subculturing was performed by incubating plates with 0.5 mM EDTA for 5 minutes and then resuspended in culture medium to be transferred to new coated plates.
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bioRxiv - Developmental Biology 2023Quote: MAN13 hESCs were expanded in 6-well cell culture plates previously coated with 5µg/ml Human Recombinant Vitronectin (Gibco, #A27940) in Dulbecco’s Phosphate Buffered Saline (PBS ...
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bioRxiv - Systems Biology 2023Quote: ... supplemented with 2.5 µg of human recombinant epidermal growth factor (rEGF) and 25 mg of bovine pituitary extract (BPE) (Gibco, 17005042). Cells were passaged at 80% confluency using TrypLE Express Enzyme (Gibco ...
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bioRxiv - Cell Biology 2023Quote: ... while islets were seeded onto the same coverslips coated with recombinant human laminin-521 (Gibco, #A29248, 0.5 μg/cm 2). Cells/islets were allowed to attach overnight ...
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bioRxiv - Cell Biology 2023Quote: The baculovirus preparation for recombinant human HAUS complex expression was carried out according to the manufacturer’s protocol (Bac-to-Bac system, Life Technologies) using E ...
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bioRxiv - Cancer Biology 2023Quote: 33G needles were filled with Matrigel and L15-BSA with or without (control) addition of 25 nM human recombinant EGF (Invitrogen). Mice were anesthetized using 5% isoflurane and laid on their back ...
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bioRxiv - Genetics 2024Quote: ... supplemented with human recombinant epidermal growth factor (EGF 30µg/ml) and bovine pituitary extract (BPE 0.2 ng/ml) (Thermo Fisher Scientific). Primary Epidermal Keratinocytes ...
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bioRxiv - Cancer Biology 2019Quote: ... These α1-α2 phage phage libraries were captured with either biotinylated Fc-iNKG2D.YA or Fc-iNKG2D.AF protein (EZ-LinkTM NHS-Biotin Kit, Thermo Fisher #20217) and enriched by cycling through four rounds of selection with increasing concentrations of non-biotinylated Fc-wtNKG2D competitor ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... The PLpro and 3CLpro proteins were biotinylated by minimal biotinylation approach with the EZ-LINK Sulfo-NHS-LC-LC-biotin reagent (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2019Quote: SiRNA-treated and PDGF-BB-stimulated cells were washed twice with cold PBS and surface proteins were biotinylated with 0.5 mg/ml Sulfo-NHS-SS-Biotin (Thermo Scientific) for 30 min on ice ...
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bioRxiv - Biochemistry 2021Quote: ... S1 subunit and the S protein (InVivo Biotech) were biotinylated using 10 molar excess of NHS-LC-Biotin (Thermo Scientific). Immunoassay plates were coated with 2.5 μg/ml recombinant angiotensin converting enzyme-2 (ACE2 ...
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bioRxiv - Biochemistry 2022Quote: ... Biotinylated samples (1.2 mg of protein) were incubated with pre-washed Neutravidin agarose beads (200 µL, 50% slurry, Thermo Scientific) in 500 µL of PBS containing 1% SDS for 120 min ...
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bioRxiv - Cell Biology 2022Quote: ... were prepared as described above except that ConA-coated beads were incubated with biotinylated BSA previously labeled with an Alexa Fluor 555 protein labeling kit (Invitrogen). Unlabeled beads (FN-coated ...
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bioRxiv - Cell Biology 2022Quote: ... FPLC-purified biotinylated proteins (see above) were incubated with streptavidin tetramers conjugated to Alexa647 Fluor (SA-647) (Thermo Fisher Scientific) at a 4:1 molar ratio on ice for at least 15 minutes ...
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bioRxiv - Neuroscience 2022Quote: ... were incubated with 5.8 μl of 1 mg/ml of PierceTM biotinylated Protein G (ThermoFisher Scientific, Waltham, MA; Cat#29988) and 20 μg of either the hnRNP H antibody (Bethyl ...
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bioRxiv - Neuroscience 2022Quote: ... as well as ensure the specificity of streptavidin- purified preparations for biotinylated proteins via silver stain (Pierce, Thermo fisher #24612) and western blot.
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bioRxiv - Cell Biology 2024Quote: EV surface proteins were captured using biotinylated using Pierce™ Cell Surface Biotinylation and Isolation Kit (A44390, Thermo Fisher Scientific), digested with trypsin (Promega ...
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bioRxiv - Immunology 2024Quote: ... antigen proteins of the target were biotinylated using the EZ-link™Sulfo-NHS-LC-LC-Biotin kit (Thermo Fisher), then coupled to the fluorescent neutravidin beads (Thermo Fisher ...
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bioRxiv - Microbiology 2023Quote: ... were biotinylated by mixing 50 μM of protein with 50 μM of EZ-Link™ Sulfo-NHS-Biotin (Thermofisher Scientific) followed by incubation on ice for 2 hours ...
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bioRxiv - Immunology 2023Quote: ... 25 µg of Spike protein was biotinylated according to the instructions of EZ-LinkTM Micro Sulfo-NHS-LCBiotinylation Kit (ThermoFisher). Then fluorescent (APC ...
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bioRxiv - Molecular Biology 2021Quote: ... Add 100 µL Pol II antibody-conjugated beads (10 µg Pol II 8WG16 antibody conjugated with Dynabeads™ Protein G for Immunoprecipitation (ThermoFisher, #1004D)) and incubate on a tube rotator for 1 hour in the 4 °C room ...
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bioRxiv - Cell Biology 2019Quote: ... 0.3μg of GFP-tagged construct DNA were mixed with 0.7μg of mchr-tagged construct DNA and diluted in Optimem (ThermoFisher) without serum or antibiotics ...
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bioRxiv - Cell Biology 2019Quote: ... 0.1μg of GFP-tagged construct DNA were mixed with 0.2μg of mchr-tagged construct DNA and diluted in Optimem (ThermoFisher) without serum or antibiotics ...
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bioRxiv - Cell Biology 2023Quote: ... Sections were then incubated with fluorescently tagged secondary antibodies and/or fluorescently tagged Phalloidin (ThermoFisher Scientific, Waltham, MA) diluted in the blocking buffer at room temperature for 1 hour ...
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bioRxiv - Cell Biology 2022Quote: ... 1µg of GFP-tagged construct DNA were mixed with 0.5µg of mchr-tagged construct DNA and diluted in Optimem (ThermoFisher) without serum or antibiotics ...
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bioRxiv - Cell Biology 2022Quote: ... of the standard curve used in cf-mtDNA/cf-nDNA assessment were measured separately using singleplex ND1 and B2M assays using a QuantStudio 3D Digital PCR System and associated reagents (Thermo Fisher, cat#A29154) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: ... of the standard curve used in cf-mtDNA/cf-nDNA assessment were measured separately using singleplex ND1 and B2M assays using a QuantStudio 3D Digital PCR System and associated reagents (Thermo Fisher, cat#A29154) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2023Quote: ... 5 ng of DNA were used for evaluating the quantity of mitochondrial MT-ND1 (VIC) and nuclear B2M (FAM) genes by qRT-PCR using TaqMan™ Gene Expression Assays (Thermo Fisher Scientific) with Taqman probes as described in (Wasner et al ...
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bioRxiv - Neuroscience 2021Quote: ... biotinylated horse anti-mouse IgG (H+L) and biotinylated goat anti-mouse IgG2a (Life Technologies, ThermoFisher Scientific).
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bioRxiv - Neuroscience 2021Quote: ... biotinylated horse anti-mouse IgG (H+L) and biotinylated goat anti-mouse IgG2a (Life Technologies, ThermoFisher Scientific).
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bioRxiv - Genomics 2020Quote: ... the biotinylated RNA was resuspended in water and biotinylated-RNA was separated with M280 Streptavidin Dynabeads (Invitrogen). 100 ul/sample of beads were washed twice with 2 volumes of freshly prepared wash buffer (100 mM Tris pH 7.5 ...
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bioRxiv - Cancer Biology 2023Quote: ... the biotinylated RNA was resuspended in water and biotinylated-RNA was separated with M280 Streptavidin Dynabeads (Invitrogen). 100 ul/sample of beads were washed twice with 2 volumes of freshly prepared wash buffer (100 mM Tris pH 7.5 ...
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bioRxiv - Biochemistry 2020Quote: ... Fluorescently tagged secondary antibodies were from ThermoFisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... Alexa 488-tagged phalloidin (ThermoFisher, 400x stock) was added at 4x working concentration and incubated for 2 days ...
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bioRxiv - Developmental Biology 2023Quote: ... Appropriate Alexa Fluor-tagged secondary antibodies (Invitrogen) were matched to the primaries and incubated for 2 h at room temperature ...
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bioRxiv - Biochemistry 2024Quote: ... FLAG-tagged Fzd4 in pcDNA3.1 (Thermo Fisher), or both Tspan12 and Fzd4 (300 ng each receptor per mL of cells) ...
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bioRxiv - Developmental Biology 2021Quote: ... HA and Myc tagged proteins were visualized on an Amersham Hyperfilm MP (28906845, Cytiva) using SuperSignal WestDuo (34075, Thermo Fisher Scientific).
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bioRxiv - Cancer Biology 2020Quote: ... cells were transfected with V5-tagged WNT11 and 24 h post transfection proteins were crosslinked for 30 min with 1 mM DSS (Thermo Fisher) in 1 ml PBS + 1mM MgCl2 ...
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bioRxiv - Genomics 2021Quote: ... MBP-tagged (pTHMT, Peti and Page, 2007) full-length CLAMP protein was expressed in Escherichia coli BL21 Star (DE3) cells (Life Technologies). Bacterial cultures were grown to an optical density of 0.7 to 0.9 before induction with 1 mM isopropyl-β-D-1-thiogalactopyranoside (IPTG ...
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bioRxiv - Biochemistry 2021Quote: ... Genes encoding pep86 (trademarked as ‘SmBiT’ (Dixon et al., 2015)) -tagged mitochondrial PCP proteins (from MGW Eurofins or Thermo Fisher Scientific) were cloned into either pBAD ...
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bioRxiv - Cell Biology 2020Quote: GST pull-downs were performed by incubating purified GST tagged proteins (5 µg) with 20 μl dry volume of glutathione agarose 4B beads (Thermo Scientific) for 1 h under rotation at 4 °C ...
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bioRxiv - Microbiology 2024Quote: ... Western blotting and immunodetection of HA-tagged proteins with anti-HA and horse radish peroxidase-coupled secondary antibodies using an iBright™ FL1500 (ThermoFisher). The remaining 25 µl bead suspension was used as input for on-bead digestion (Klaus et al. ...
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Identification of plants functional counterparts of the metazoan Mediator of DNA Damage Checkpoint 1bioRxiv - Cell Biology 2023Quote: ... GST-tagged proteins (5 µg) along with GST alone were incubated with 10 µl of magnetic glutathione beads (Thermo Fisher Scientific) for one hour at RT ...
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bioRxiv - Molecular Biology 2023Quote: ... or 90 pmol of SBP-tagged eIF4A1 protein was incubated with 30 μl of Dynabeads M-270 Streptavidin (Thermo Fisher Scientific), which had been preequilibrated with equilibration buffer (20 mM Tris-HCl pH 7.5 ...