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Citations for Thermo Fisher :
2101 - 2150 of 10000+ citations for Recombinant Human FCGRT & B2M Protein His Avi Strep II tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... CuAAC reaction was performed as previously described23 and biotinylated proteins were enriched using Pierce™ streptavidin agarose beads (Cat. # 20353, ThermoFisher).
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bioRxiv - Cell Biology 2023Quote: ... Biotinylation and purification of biotinylated proteins were done following the Pierce™ Cell Surface Biotinylation and Isolation Kit’s (A44390, Thermo Scientific) protocol ...
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bioRxiv - Physiology 2024Quote: ... Biotinylated proteins were precipitated from the remaining lysate by overnight incubation at 4°C with streptavidin agarose resin (Thermo Scientific, #20353) and recovered in 4x loading sample buffer (0.2M Tris-HCl pH 6.8 ...
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bioRxiv - Immunology 2023Quote: ... The monomer form of the antigens was biotinylated and the subsequent tetramerization of these proteins was done with FITC-labeled avidin (Invitrogen, A821), in brief ...
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bioRxiv - Microbiology 2023Quote: ... 0.4pmol of biotinylated SINV RNA was incubated with normalized amounts of protein lysates and RNA binding buffer containing RNaseOUT (Thermo Fisher), heparin (Sigma-Aldrich) ...
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bioRxiv - Cancer Biology 2024Quote: ... and clear supernatant were collected and proceeded with streptavidin pull down to enrich biotinylated proteins as per the manufacturers protocol (#Thermofisher scientific). On column trypsin digestion were performed on the samples ...
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bioRxiv - Immunology 2024Quote: ... 50 μL lysate (∼25 ug total protein) was mixed with 2 μg 5’-biotinylated dsDNA probe and 50 μL streptavidin agarose resin (Thermo Scientific) in PBS containing protease inhibitors (Roche ...
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bioRxiv - Microbiology 2019Quote: ... Recombinant RNase H (Invitrogen) was later added to the reverse transcribed samples and incubated for 20 min at 37°C ...
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bioRxiv - Immunology 2022Quote: ... Recombinant B18R (Life Technologies) was used at 1ug/ml ...
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bioRxiv - Neuroscience 2024Quote: ... Recombinant PAK6 (ThermoFisher, #PR5307A) was diluted in blocking buffer and incubated with the arrays at room temperature (RT ...
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bioRxiv - Pathology 2022Quote: ... Protein concentration was determined with Bio-Rad Protein Assay Kit II and 15 μg sample protein was loaded in a NuPAGE 3-8 % Tris-Acetate gel (Thermo Fisher). The gel was run at a constant voltage of 150 V for 4 h ...
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bioRxiv - Neuroscience 2024Quote: ApoE and sAPP were measured in human CSF samples using the Apolipoprotein E Human ELISA Kit (#EHAPOE) and Amyloid Precursor Protein Human ELISA Kit (#KHB0051) kits respectively from Life Technologies, following the manufactureŕs protocol in duplicates using a 1:100 dilution of CSF.
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bioRxiv - Microbiology 2021Quote: ... Biotinylated double-stranded were synthesized probes (ThermoFisher) with the indicated sequence.
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bioRxiv - Bioengineering 2020Quote: ... and biotinylated detection antibody pairs from Invitrogen™ ...
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bioRxiv - Biophysics 2019Quote: ... 0.2 mg ml−1 biotinylated BSA (ThermoFisher) was added into the chamber and incubated for 3 min ...
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bioRxiv - Systems Biology 2019Quote: ... 4 µl Biotinylated Cytidine (Thermo Fisher, 1mM), 2 U RNase inhibitor ...
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bioRxiv - Molecular Biology 2023Quote: ... and biotinylated cytidine (bis)phosphate (Thermo scientific) using T4 RNA ligase enzyme from Thermo Fisher Scientific.
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bioRxiv - Biochemistry 2023Quote: ... followed by biotinylated BSA (Life Technologies Europe) diluted in BRB80 ...
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bioRxiv - Bioengineering 2024Quote: ... Biotinylated α-Bungarotoxin (BTX) (Invitrogen, cat#: B1196) was included in the primary antibody diluent (1 μg mL-1 ...
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bioRxiv - Immunology 2024Quote: ... biotinylated secondary antibody (Invitrogen, Massachusetts, United States) diluted 1:8000 in reagent diluent was added for 1 hour at 37°C ...
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bioRxiv - Neuroscience 2019Quote: ... All proteins were expressed in Human Embryonic Kidney (HEK) 293 Freestyle cells (Invitrogen) in suspension culture using serum-free media ...
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bioRxiv - Immunology 2023Quote: ... RBD proteins were expressed in-house in Expi293F human cells (Thermo Fisher Scientific) by transfection of the cells with purified DNA and polyethylenimine (PEI) ...
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bioRxiv - Biochemistry 2023Quote: Proteins from human cell lines were extracted in IP lysis buffer (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... AHA-labeled proteins were detected with 1 μM alkyne-tagged Alexa Fluor-488 using Click-iT cell detection reagents (Thermo Fisher Scientific) for 45 min at room temperature as previously described (3).
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bioRxiv - Neuroscience 2021Quote: Bacterial expression constructs coding for His6/SUMO-tagged fusion proteins of rat Shank3 (residues 1-348, SPN-Ank) were generated in pET-SUMO (Thermo Fisher Scientific) as described before (17) ...
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bioRxiv - Molecular Biology 2021Quote: ... Co-purification of 6His-tagged PGC-1α protein domains was detected by western blot using anti-6His antibody (clone His.H8, ThermoFisher Scientific MA1-21315).
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bioRxiv - Cell Biology 2021Quote: ... and anti-TRIP4 antibodies were raised in rabbits against affinity purified N-terminally hexahistidine-tagged full-length proteins and purified with the respective antigens coupled to SulfoLink resin (Thermo Fisher Scientific).
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bioRxiv - Cell Biology 2022Quote: ... SH-SY-5Y cells stably expressing GFP or a GFP-tagged construct of the protein of interest were cultured for at least six doublings in SILAC DMEM (89985; Thermo Fisher Scientific) supplemented with 10% dialyzed FBS (F0392 ...
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Srs2 binding to PCNA and its sumoylation contribute to RPA antagonism during the DNA damage responsebioRxiv - Molecular Biology 2024Quote: ... The activated form of Mec1 was enriched by immunoprecipitating myc-tagged Ddc2 using Protein A beads and anti-Myc antibody (Thermo Fisher 9E10) for 2–4 h at 4°C ...
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bioRxiv - Molecular Biology 2023Quote: ... GST pull-down of PARylated proteins using GST-tagged RNF146(100-182) or GST-tagged AF1521(K35E/Y145R) was done using the Pierce GST protein interaction pull-down kit (Thermo Fisher Scientific). Glutathione agarose resin (50μl ...
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bioRxiv - Systems Biology 2023Quote: ... ABE8e was expressed as an 8xHis tagged protein from a rhamnose-inducible promoter in BL21-Star DE3 cells with low RNase activity (Thermo Fisher Scientific). At an OD600 of ∼0.8 ...
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bioRxiv - Biophysics 2024Quote: RBL-2H3 cells stably expressing clathrin light chain A tagged with green fluorescent proteins were cultured in minimum essential medium (MEM) (Invitrogen 11095-09) with 20% fetal bovine serum (FBS ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were transfected with pDisplay encoding myc-tagged GluA1-GluA4-ATD or pCAGGS encoding FLAG-tagged Nrx or GFP with Lipofectamin 2000 (Invitrogen). On the following day ...
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bioRxiv - Microbiology 2020Quote: Human IFITM proteins and SARS-CoV-2 viral proteins were cloned into MaMTH N-term tagged Prey and C-term tagged Bait vectors respectively using Gateway cloning technology (ThermoFisher). Correctness of recombined insertions was confirmed by Sanger sequencing (Eurofins) ...
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bioRxiv - Cell Biology 2023Quote: U2OS were transfected with the MAC-tagged GTPBP8 expression vector or a control MAC-tagged GFP vector using Fugene 6 (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: GST-tagged full-length SYK and VenusFlag-tagged full-length FCER1G were generated using Gateway LR Clonase (Invitrogen, 11791-100) for mammalian cell expression ...
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bioRxiv - Molecular Biology 2022Quote: Insect or bacterially expressed proteins were purified by His-tag purification using hiTRAP FF columns (GE) or HisPur Resin (Thermo Scientific), followed by His tag removal by Tobacco Etch Virus (TEV ...
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bioRxiv - Microbiology 2021Quote: ... recombinant SARS-CoV-2 Spike RBD protein (RBD, His Tag) was conjugated with EZ-LinkTM Sulfo-NHS-Biotin (1:3 molar ratio; Thermo Fisher) in Dulbecco’s PBS at room temperature for 30 min ...
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bioRxiv - Biochemistry 2023Quote: ... Cell lysates were cleared by centrifugation at 20,000 rpm for 45 min and the His-SUMO fusion proteins were enriched on Ni-NTA beads (Thermo Fisher Scientific). The protein was eluted using a buffer containing 25 mM Tris (pH 8.0) ...
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bioRxiv - Biochemistry 2024Quote: Purified Sl-Enc1/His-Sl-Enc2 protein samples were analyzed via SDS-PAGE with a 14% Novex Tris-Glycine Gels (Thermo-Fisher Scientific). The SDS-PAGE gel was stained with Coomassie and the overexpressed bands at the correct molecular weights were excised and stored in water ...
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bioRxiv - Biochemistry 2022Quote: ... The Z-scores for reactivity of SARS CoV-2 proteins to each human protein was determined by using ProtoArray Prospector v 5.2 software (Invitrogen, Thermo Fisher).
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bioRxiv - Molecular Biology 2020Quote: ... Alexa555 or Alexa 594 tagged secondary antibodies (Invitrogen) were used ...
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bioRxiv - Cell Biology 2019Quote: ... fluorescent Alexa-tagged antibodies were from Molecular Probes.
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bioRxiv - Neuroscience 2020Quote: ... fluorescently-tagged polystyrene microspheres (10 micron, Invitrogen F8836) were bilaterally injected into the anterior chambers of Opn4Cre;Ai32 mice at ~6-8 weeks of age (Sappington et al. ...
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bioRxiv - Microbiology 2021Quote: ... Fluorescence-tagged secondary antibodies were purchased from Thermofisher and used at 1:500 dilutions ...
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bioRxiv - Cell Biology 2021Quote: ... horseradish peroxidase-tagged second antibody (Thermo Fisher Scientific) was applied for 1 h at room temperature and the signal visualized using SuperSignal enhanced chemiluminescent (ECL ...
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bioRxiv - Microbiology 2021Quote: ... and Alexa488-tagged phalloidin were obtained from Invitrogen and were used at 1:1000 dilution.
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bioRxiv - Molecular Biology 2020Quote: ... fluorescent Alexa-tagged antibodies were from Molecular Probes. VOA ...
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bioRxiv - Cancer Biology 2022Quote: ... Alexa555 or Alexa 594-tagged secondary antibodies (Invitrogen) were used ...
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bioRxiv - Developmental Biology 2022Quote: ... An appropriate Alexa Fluor-tagged secondary antibody (Invitrogen) was matched to the primary and incubated for 1-2 h at room temperature ...