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Citations for Thermo Fisher :
1851 - 1900 of 10000+ citations for Recombinant Human FCGRT & B2M Protein His Avi Strep II tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2024Quote: ... and 1% penicillin/streptomycin (Penn/Strep, Thermo Fisher). WI-38 (Coriell Institute ...
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bioRxiv - Neuroscience 2024Quote: ... and 1% penicillin-streptomycin (Pen-Strep, Life Technologies) at 37°C in a 5% (vol/vol ...
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bioRxiv - Neuroscience 2024Quote: ... + 0.1% Pen/Strep (P/S; Fisher Scientific, 11548876), with daily full medium refreshments ...
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bioRxiv - Cancer Biology 2024Quote: ... and 1% (v/v) Pen/Strep (#15070063, Invitrogen). FT194 and FT282 were maintained in phenol red free DMEM/F12 medium containing 10% (v/v ...
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bioRxiv - Developmental Biology 2024Quote: ... 0.5% Pen-Strep (Life Technologies, Carlsbad, CA, US), and 1% FBS (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2024Quote: ... 50 μg/ml streptomycin (pen/strep, Thermo Fisher), 10 mM HEPES (Sigma) ...
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bioRxiv - Cell Biology 2023Quote: Human ApoE protein was quantified using the Invitrogen Human ApoE ELISA following the manufacturers guidelines (Invitrogen. Cat no. EHAPOE).
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bioRxiv - Plant Biology 2021Quote: ... Inserts from the recombinant pENTR plasmids were transferred to destination vectors via gateway cloning by using the LR clonaseTM II Enzyme mix (Invitrogen, USA) and by following the manufactureŕs instructions ...
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bioRxiv - Plant Biology 2020Quote: ... cDNAs from pUC57 entry vector were transformed by electroporation into Escherichia coli strain DH10B and transferred by a recombinant LR reaction of Gateway cloning (Clonase II enzyme mix Kit, Thermo Fisher) into pB7WGF2 (Karimi et al. ...
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bioRxiv - Bioengineering 2021Quote: ... and 1% (v/v) PFHM-II protein-free hybridoma medium (Thermo Fisher Scientific). On day 4 of differentiation ...
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bioRxiv - Immunology 2022Quote: ... and GK1.5 hybridomas were grown in PFHM-II Protein-Free Hybridoma Medium (Thermofisher) (Dialynas et al. ...
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bioRxiv - Bioengineering 2020Quote: ... and 1% (v/v) PFHM-II protein-free hybridoma medium (Thermo Fisher Scientific). On day 4 of differentiation ...
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bioRxiv - Immunology 2020Quote: ... The 8A7H5 hybridoma was maintained in PFHM-II Protein-Free Hybridoma Medium (ThermoFisher) at 37°C in 5% CO2 ...
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bioRxiv - Molecular Biology 2023Quote: ... 3 × 10-4 M MTG and 5% Protein-Free Hybridoma Media II (Gibco).
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bioRxiv - Neuroscience 2023Quote: ... 1% Pen-Strep by volume (v/v)) at 37 °C/5% CO2 in chambered slides (Thermo Fisher Scientific Nunc Lab-Tek II CC2 Chamber Slide, Z734853) at either 10K microglia (10,000 microglia/well or 50,000 microglia/mL ...
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bioRxiv - Biochemistry 2020Quote: ... biotinylated (Cat.# 65-6140; Thermo Fisher) and Alexa Fluor 647-conjugated (Cat.# ab150079 ...
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bioRxiv - Immunology 2019Quote: ... biotinylated secondary antibodies (1:500, Invitrogen) were added and incubated at RT for 1hr ...
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bioRxiv - Biochemistry 2021Quote: ... biotinylated phycoerythrin from Invitrogen (Eugene, OR) and underivatized streptavidin from Thermo Fisher ...
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bioRxiv - Neuroscience 2021Quote: ... AT180 and HT7 (biotinylated) (Thermo Scientific) 1:10 ...
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bioRxiv - Cell Biology 2024Quote: ... and biotinylated CD56 (Thermo Fisher Scientific) were added to the well ...
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bioRxiv - Immunology 2023Quote: ... Monomeric antigens were biotinylated (ThermoFisher; A39257) at an optimized ratio which resulted in <1 biotin per molecule ...
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bioRxiv - Immunology 2023Quote: ... was biotinylated (ThermoFisher Scientific, Waltham, MA) and tetramerized with streptavidin-PE or streptavidin-APC (Agilent ...
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bioRxiv - Developmental Biology 2021Quote: ... The ORF of red fluorescent protein gene (RFP)-fused Antp was inserted into a pIZ/V5-His vector (Invitrogen) driven by the OpIE2 promoter ...
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bioRxiv - Biochemistry 2020Quote: ... Binding of the full-length S protein was detected by mouse anti-His IgG (Invitrogen MA121315, 2 μg/ml) and Alexa Fluor-488-labelled goat anti-mouse IgG (Invitrogen A11001 ...
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bioRxiv - Immunology 2022Quote: ... Protein expression was confirmed by western blot using a 6x His-Tag HRP conjugated Monoclonal Antibody (Thermo Fisher Scientific). Once verified ...
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bioRxiv - Microbiology 2019Quote: Bacterially expressed ZIKV EDIII proteins (C-terminal 6 × His-tag) were conjugated to Ni-NTA Magnetic beads (Thermo Scientific) following manufacturers protocol ...
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bioRxiv - Plant Biology 2023Quote: ... and proteins were visualized using Ponceau stain as well as Western blot with monoclonal anti-His (Invitrogen MA1-21315), and polyclonal anti-GST (Thermo Scientific ...
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bioRxiv - Systems Biology 2023Quote: ... and utilized Alt-R CRISPR-Cas9 crRNA/trRNA/Hi-Fi Cas9 ribonucleotide-protein complex (IDT) and Neon electroporation (ThermoFisher) to deliver the complex to the iPSC ...
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bioRxiv - Immunology 2023Quote: ... Protein detection was performed by incubation with a 6x-His Tag mouse monoclonal antibody (clone: HIS.H8; Thermo Fisher Scientific), followed by a secondary goat-anti-mouse HRP antibody (clone NA9310 N ...
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bioRxiv - Immunology 2023Quote: ... Proteins containing a His-tag were purified by affinity chromatography using HisPur Ni-NTA resin (Thermo scientific, Cat#88222). Proteins were then eluted with 250 mM imidazole in 50 mM Tris-HCl and 300 mM NaCl ...
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bioRxiv - Molecular Biology 2020Quote: ... Plates were washed and 50μl Pierce recombinant protein A/G with peroxidase (Thermo Fisher catalog no: 32490) added at 1:10,000 in PBS-T with 1% milk as a secondary and incubated 1 hour at room temperature ...
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bioRxiv - Biochemistry 2020Quote: ... Concentration of the recombinant peptides was determined using Pierce BCA Protein Assay Kit (Thermo Fisher Scientific, USA) with bovine serum albumin as the standard.
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bioRxiv - Plant Biology 2021Quote: For recombinant protein expression in Pichia pastoris GS115 (Multi-Copy Pichia Expression Kit Instructions, Thermo Fisher Scientific), constructs of IF1 ...
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bioRxiv - Microbiology 2020Quote: ... Recombinant forms of SyAM in culture supernatant were isolated by protein A affinity resins (MabCapture, ThermoFisher Scientific) using Pierce gentle Ag/Ab elution buffer (ThermoFisher Scientific) ...
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bioRxiv - Immunology 2021Quote: ... previously cultured for 24h with IL-15/IL-15R complex recombinant protein (100ng/ml, Thermo Fisher Scientific) and retinoic acid (100ng/ml) ...
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bioRxiv - Microbiology 2020Quote: The recombinant proteins were purified using nickel sepharose beads (Ni-NTA, Thermo Fisher Scientific, Waltham, MA, USA). The lysate supernatant from the 50 ml culture was mixed with 300 μl Ni-NTA beads and incubated overnight at 4° in the lysis buffer supplemented with 5 mM imidazole ...
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bioRxiv - Immunology 2022Quote: Recombinant tri-S protein was heat-denatured at 100°C for 3 min in loading buffer (Invitrogen) containing 1X sample reducing agent (Invitrogen) ...
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bioRxiv - Pathology 2020Quote: ... A 1ug/mL mixture of 50μL per well containing recombinant protein in carbonate buffer (0.1M Na2CO3 0.1M NaHCO3 pH 9.3) was used to coat MaxiSorp (ThermoFisher) 96-well plates overnight at 4°C ...
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bioRxiv - Immunology 2020Quote: ... Endotoxin was removed from recombinant protein using High Capacity Endotoxin Removal Spin Columns (ThermoFisher Scientific Catalog #88274) following the manufacturer’s instructions.
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bioRxiv - Immunology 2020Quote: ... Recombinant proteins and antibody 47D11 were expressed transiently in FreeStyle™ 293-F Cells (Thermo Fisher Scientific) and affinity purified from the culture supernatant by protein-A sepharose beads (GE Healthcare ...
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bioRxiv - Microbiology 2022Quote: All recombinant proteins were expressed and purified using a mammalian expression system via Expi293F cells (Life Technologies), as described in detail earlier (30 ...
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bioRxiv - Plant Biology 2022Quote: For recombinant protein expression in Pichia pastoris KM71H (Multi-Copy Pichia Expression Kit Instructions, Thermo Fisher Scientific), constructs encoding SCPSs ...
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bioRxiv - Biochemistry 2023Quote: ... 1 mg/mL of recombinant protein was mixed with SYPRO Orange at a 5X final concentration (Invitrogen), and reactions were supplemented with 50 mM HEPES ...
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bioRxiv - Cell Biology 2023Quote: ... and recombinant Streptococcus pyogenes Cas9 protein (SpCas9; Synthego) using Lipofectamine CRISPRMAX Cas9 Transfection reagent (CMAX00001; Thermo Fisher) according to the manufacturer protocol ...
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bioRxiv - Cell Biology 2023Quote: ... Recombinant proteins were labelled by conjugation with either Alexa Fluor 488 or 647 following maleimide chemistry (Invitrogen), as in (Picas et al. ...
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Structural and mechanistic insights into disease-associated endolysosomal exonucleases PLD3 and PLD4bioRxiv - Biochemistry 2023Quote: Recombinant PLD3 and PLD4 proteins were expressed in Expi293F cell line according to the manufacturer’s instructions (ThermoFisher). In brief ...
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bioRxiv - Microbiology 2020Quote: rCEACAM-His was attached to nickel dynabeads (DB; Dynabead His-tag pull-down & isolation, Invitrogen) following standard protocols ...
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bioRxiv - Immunology 2022Quote: Expression vectors for human His-NLRP1PYD (aa 1-92) and GST-NLRP1PYD were generated by Gateway cloning with destination vector pDEST17 (Thermo Fisher Scientific) and a customized destination vector based on pGEX-2T (a kind gift of Mikko Taipale ...
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bioRxiv - Cell Biology 2023Quote: ... The plasmid pcDNA4-B–hVMP1–V5/His was obtained by inserting the human VMP1 sequence (NM_030938.5) into pcDNATM4/V5-HisB (Invitrogen catalog number V861-20). pLenti–hVMP1–FLAG was obtained by inserting the oligonucleotide MCS-flag into pENTR1A (Addgene Plasmid #17398) ...
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bioRxiv - Biochemistry 2024Quote: ... HER2 with a C-terminal tail truncation (λ−1030–1255) followed by maltose binding protein (MBP) and twin-strep tags was cloned into pFastBac1 with a CMV promoter (Thermo Fisher Scientific). Point mutations were introduced in the HER2 kinase domain ...