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Citations for Thermo Fisher :
1701 - 1750 of 10000+ citations for Recombinant Human FCGRT & B2M Protein His Avi Strep II tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2021Quote: ... mCherry-tagged protein-expressing Neuro2a cells were incubated in a medium containing 0.5 μM Lysotrascker green DND-26 (Thermo Fisher) for 30 min before image acquisition.
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bioRxiv - Developmental Biology 2021Quote: Immunoprecipitations from HEK293T cells expressing either HA-or FLAG-tagged Yap were performed using the Dynabeads Protein A Immunoprecipitation Kit (Invitrogen) by coupling 5 μg of anti-FLAG (Cell signaling ...
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bioRxiv - Molecular Biology 2020Quote: ... Large-scale expression of Strep-tagged proteins for purification was conducted in Trichoplusia Ni High Five cells (Thermo Fischer Scientific) in SF-900 medium (Gibco). Cells at a density of 2×106 cells/ml were infected with 1.5% of amplified virus and incubated for 40 hours ...
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bioRxiv - Cell Biology 2020Quote: ... Mouse IP6K1 was sub-cloned into SFB (S-protein/FLAG/SBP) - tagged destination vector using the Gateway cloning strategy (Invitrogen). Catalytically inactive IP6K1 (K226A ...
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bioRxiv - Biochemistry 2020Quote: MBP-tagged (pTHMT) full-length SARS-CoV2 nucleocapsid protein was expressed in Escherichia coli BL21 Star (DE3) cells (Life Technologies). Bacterial cultures were grown to an optical density of 0.7 to 0.9 before induction with 1 mM isopropyl-β-D-1-thiogalactopyranoside (IPTG ...
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bioRxiv - Synthetic Biology 2021Quote: ... Clarified lysates containing His6x-tagged proteins were subjected to gravity-flow Ni2+-affinity purification using HisPur Ni-NTA Resin (ThermoFisher) following manufacturer’s protocols ...
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bioRxiv - Microbiology 2021Quote: ... nucleotide sequences encoding N-terminal FLAG-tagged VACV proteins were subcloned between the BamHI and XbaI restriction sites of a pcDNA4/TO vector (Invitrogen). Alternatively ...
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bioRxiv - Molecular Biology 2020Quote: ... The Mex67-3Flag tagged protein was isolated using 200 µL of pre-washed magnetic Pan Mouse Dynabeads (Thermo Fisher Scientific) coupled to 34 µg of anti-Flag M2 mouse monoclonal antibody (Sigma) ...
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bioRxiv - Microbiology 2020Quote: Expression vectors for FixL and FixJ His6-tagged proteins were generated using the Gateway high-throughput recombinational cloning system (Invitrogen). The entire fixJ gene or fixL amino acids 329-851 (lacking transmembrane domains ...
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bioRxiv - Microbiology 2020Quote: ... The HA-tagged proteins were captured with the Pierce™ HA-Tag Magnetic IP/Co-IP Kit (Thermo Scientific™). Magnetic beads (0.25 mg ...
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bioRxiv - Molecular Biology 2020Quote: ... The entry clones were recombined into the Gateway binary vector pGWB552 tagged with G3 green fluorescent protein (G3GFP) using LR clonase (Invitrogen) (Nakagawa et al. ...
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bioRxiv - Molecular Biology 2020Quote: ... the receptor Fc-tagged ectodomains present in the conditioned media were captured on protein A-coated 384-plates (Thermo Scientific), and stored at 4 °C until use ...
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bioRxiv - Cell Biology 2020Quote: Bacterial expression constructs coding for His6/SUMO tagged fusion proteins of rat SHANK3 were generated in pET-SUMO (Thermo Scientific) as described 14 ...
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bioRxiv - Molecular Biology 2021Quote: ... For Protein A-tagged strains, immunoprecipitation was carried out with Rabbit IgG-conjugated (MP-Biomedicals, SKU 085594) Dynabeads (Invitrogen, 14301) as described 57 ...
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bioRxiv - Microbiology 2020Quote: HEK293 were transfected with 3xFlag-tagged plasmids encoding each SARS-CoV-2 protein by Lipofectamine 3000 (Thermo Fisher Scientific, #L3000015). 3×107 HEK293 cells were harvested in 10 ml lysis buffer (50 mM Tris-HCl [pH 7.5] ...
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bioRxiv - Microbiology 2021Quote: ... concentrated eluates issued from immunopurification of endogenous and RACK1-HA-tagged protein are separated on 10% Tris-glycine SDS-PAGE gels (Invitrogen), and stained with Imperial Protein Stain (Thermo Fisher) ...
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bioRxiv - Cell Biology 2024Quote: ... human protein database with common lab contaminants and the sequence of the tagged bait proteins added using SEQUEST within Proteome Discoverer 1.4 (Thermo Fisher). The search parameters were as follows ...
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bioRxiv - Cell Biology 2024Quote: ... the GFP-tagged proteins were immunoprecipitated by incubating the extracts with 15 μl of anti-HA magnetic beads (Thermo Scientific) for 2 h at 4°C with gentle shaking ...
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bioRxiv - Biochemistry 2023Quote: ... FLAG-tagged proteins were then eluted with room temperature Elution Buffer with 2.5 mg/mL 3XFLAG Peptide (Thermo Fisher # A36806) for 15 min at room temperature ...
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bioRxiv - Biochemistry 2022Quote: The MS/MS spectra were searched against the NCBI Mycobacterium smegmatis database with common lab contaminants and the sequence of the tagged bait proteins were added using SEQUEST within Proteome Discoverer 1.4 (Thermo Fisher). The search parameters were as follows ...
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bioRxiv - Biochemistry 2024Quote: Live microscopy of parasites expressing GFP-tagged proteins was performed by staining mitochondria with 25 nM MitotrackerRed CMXRos (Invitrogen, M7512) for 30 minutes and staining nuclei with 5 µg/mL Hoechst 33342 (Invitrogen ...
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bioRxiv - Genomics 2023Quote: ... in-house biotinylated Anti-CD6 or in-house biotinylated Anti-CD27 into streptavidin-coated plates (ThermoFisher) at concentrations of 0.1 ...
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bioRxiv - Cancer Biology 2021Quote: ... 1 % pen/strep and 100 µM Glutamax (Invitrogen) at 37 °C and 5 % CO2 ...
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bioRxiv - Cell Biology 2020Quote: ... 1% HEPES and 1% Pen-Strep solution (Thermofisher) for 5 days ...
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bioRxiv - Genetics 2021Quote: ... and Pen/strep (100 U/ml) (Invitrogen, UK)] and plated out at a density of ∼80000 viable cells/cm2on poly-L-lysine (20µg/ml ...
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bioRxiv - Genomics 2021Quote: ... containing 10% FBS and 5% pen/strep (ThermoFisher). Cells were counted with a hemocytometer and Seq-Well S3 was performed as described below43 ...
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bioRxiv - Neuroscience 2020Quote: ... 50U/ml Pen/Strep (Life Technologies,17502-048), 100ng/ml IL-34 (Peprotech ...
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bioRxiv - Developmental Biology 2020Quote: ... 1%Pen/Strep and 1% Geneticin (15140122, Invitrogen). 24 hours prior to transfection ...
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bioRxiv - Developmental Biology 2020Quote: ... and 1/100 (v/v) pen/strep (Gibco) at 37°C and 5% CO2 until they reach confluency ...
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bioRxiv - Molecular Biology 2022Quote: ... and 1% Penicillin-Streptomycin (Pen Strep, Gibco 15140). 293T cells were grown in Dulbecco’s Modified Eagle Medium (DMEM ...
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bioRxiv - Cancer Biology 2022Quote: ... 1% pen/strep and 1% L-glutamine (Invitrogen). Cell lines were regularly tested for mycoplasma using the MycoAlert Mycoplasma Detection Kit (Lonza ...
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bioRxiv - Cell Biology 2019Quote: ... 100 mg/mL streptomycin (Pen-Strep, Life Technologies), and 10% v/v Fetal Bovine Serum (FBS ...
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bioRxiv - Genetics 2019Quote: ... and 1% pen/strep (P/S, Thermo Fisher). Vectors were added to HEK293T cells in growth medium plus 25uM chloroquine ...
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bioRxiv - Cancer Biology 2019Quote: ... and 1% penicillin-streptomycin (Pen-Strep; Invitrogen, ThermoFisher). All cells were treated with Plasmocin (Invivogen ...
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bioRxiv - Cancer Biology 2019Quote: ... and 1% penicillin-streptomycin (Pen-Strep; Invitrogen, ThermoFisher). All cells were treated with Plasmocin (Invivogen ...
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Transcriptomic and morphological response of SIM-A9 mouse microglia to carbon nanotube neuro-sensorsbioRxiv - Bioengineering 2020Quote: ... and 1x pen-strep-glutamine (Gibco, Life Technologies). All sera obtained were heat inactivated ...
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bioRxiv - Immunology 2021Quote: ... 1% Pen/Strep and 1% L-glutamine (Gibco), and 2.5 ng/ml recombinant mouse IL-15 and 100 units of IL-2 (R&D Systems).
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bioRxiv - Genetics 2019Quote: ... 1% L-glutamine and 1% Pen-Strep (Gibco). Patient-derived fibroblasts were cultured in DMEM (Gibco ...
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bioRxiv - Genetics 2019Quote: ... 1% L-glutamine and 1% Pen-Strep (Gibco). We seeded 500,000 HAP1 cells for transfection in 2mL of media in six-well plates using Lipofectamine 3000 (Thermo Fisher) ...
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bioRxiv - Biophysics 2021Quote: ... 1% penicillin/streptomycin (Pen/Strep) (15140122, Gibco, US), and 1% GlutaMAX (35050061 ...
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bioRxiv - Biochemistry 2021Quote: ... and 1%Pen-Strep (Gibco cat#15140-122). Lentiviral transduction was performed as follows ...
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bioRxiv - Biochemistry 2021Quote: ... and 1%Pen-Strep (Gibco cat#15140-122). Lentiviral particles were generated by co-transfection of 4 plasmids into 293FT cells using TransIT-X2 Transfection reagent ...
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bioRxiv - Biochemistry 2020Quote: ... 1% (v/v) penicillin/streptomycin (Pen/Strep) (Invitrogen) and 10% (v/v ...
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bioRxiv - Biochemistry 2020Quote: ... 1% (v/v) penicillin/streptomycin (Pen/Strep) (Invitrogen) and 10% (v/v ...
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bioRxiv - Neuroscience 2020Quote: ... 1% Pen/Strep Minimal Essential Media (MEM) (Gibco). Hippocampi were manually dissociated by trituration and diluted to a density of 50,000 cells/well and plated on poly-D-Lysine (20μg/ml ...
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bioRxiv - Microbiology 2021Quote: ... and 1% Pen/Strep (Gibco Cat# 15140-122)) supplemented with 20 IU/mL rhIL-2 (R&D Systems Cat# 202-IL ...
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bioRxiv - Cancer Biology 2020Quote: ... and 50units/mL Pen Strep (Gibco 15140-122). Transfections were done with pCDNA3.1 recombinant plasmid ...
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bioRxiv - Cell Biology 2021Quote: ... 1% (v/v) Pen/Strep (Thermo Fisher Scientific), 12.5% (v/v ...
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bioRxiv - Cell Biology 2021Quote: ... and 1x P/S Pen/Strep (Life Technologies). Low retention pipette tips and utilization of tip graduations were found recommendable for NFC and Matrigel pipetting ...
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bioRxiv - Cell Biology 2021Quote: ... 10% hiFBS (Fisher) and 1% Pen/Strep (Invitrogen), supplemented with GMCSF 10 ng/ml ...