Labshake search
Citations for Thermo Fisher :
1851 - 1900 of 10000+ citations for 8 4 chlorophenyl 8 oxooctanoic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2022Quote: ... Lysates were boiled at 95 °C for 5 min and separated by SDS–PAGE electrophoresis using 8-16% Tris-Glycine gels (Invitrogen) at 225 V for 30 min ...
-
bioRxiv - Cell Biology 2022Quote: ... or 1 μg of Super8-FOPflash (8 mutant TCF-binding sites) reporter plasmids (kindly provided by Randall Moon, University of Washington, Seattle) using Lipofectamine 2000 (Invitrogen); a thymidine kinase-Renilla plasmid (0.1 μg ...
-
bioRxiv - Immunology 2021Quote: ... 1% PenStrep and 8 μg/mL puromycin (to ensure retention of TMPRSS2) with 5% CO2 in a 37°C incubator (ThermoFisher). Cells were trypsinized using 0.05% trypsin and plated to 40,000 cells/well ...
-
bioRxiv - Immunology 2021Quote: ... The Matrigel was allowed to solidify for 8-15 minutes in a 37C incubator and solidified domes were cultured in Advanced DMEM (Gibco) media supplemented with recombinant murine Chiron 10 μM (Stemgent) ...
-
bioRxiv - Systems Biology 2019Quote: ... Metabolites were detected and quantified as area under the curve based on retention time and accurate mass (≤ 8 ppm) using the TraceFinder 3.3 (Thermo Scientific) software ...
-
bioRxiv - Immunology 2019Quote: ... were labelled for 20 minutes at room temperature with Cell Trace Violet (CTV, thermofisher) in PBS and cultured at a density of 8×104 cells/well in RPMI 1640 medium (Thermofisher) supplemented with 10% FBS (ThermoFisher) ...
-
bioRxiv - Immunology 2019Quote: Cells acquired by BAL were spun down and resuspended in complete RPMI without antibiotics and plated on a Lab-Tek II 8-chamber glass-bottom slide (Nunc). The slides were kept for 2 hours in a 37°C humidified incubator (5% CO2 ...
-
bioRxiv - Cell Biology 2019Quote: ... 8×106 HEK293T cells were seeded onto a 15 cm diameter tissue culture dish in 20ml DMEM with Glutamax (Gibco) with 10% heat inactivated FBS (Gibco) ...
-
bioRxiv - Genomics 2019Quote: ... The samples were cooled down to RT before loading them on a 3-8% Tris-Acetate Protein Gels (Invitrogen EA0375PK2). Next ...
-
bioRxiv - Biophysics 2020Quote: ... The CE was then loaded onto 8 ml prewarmed 7.5% sucrose solution in extraction buffer and microtubules pelleted at 40,000 × g (SS34 rotor, Thermo Fisher Scientific) for 30 minutes at 37 °C ...
-
bioRxiv - Cancer Biology 2019Quote: Thymus and femurs from 6-8 week old mice were harvested and subjected to red blood cell lysis (00-4333-57, Invitrogen). Thymocytes and bone marrow cells were counted by Beckman Coulter Z1 counter ...
-
bioRxiv - Cancer Biology 2019Quote: ... and primary fibroblasts obtained from lungs of healthy NSG or BALB/c mice 6-8 weeks of age were cultured in Minimum Essential Medium Eagle medium with alpha modification (MEMα) (ThermoFisher) supplemented with 1x MEM Non-essential Amino Acid Solution (Sigma-Aldrich) ...
-
bioRxiv - Immunology 2019Quote: ... mixtures were subjected to 8% SDS-PAGE with 6 µl of molecular weight marker (SeeBlue Plus2 Pre-Stained Standard, Invitrogen), after which proteins were transferred to nitrocellulose membranes (Amersham Hybond ECL 0.45 µm Nitrocellulose Membrane ...
-
A general role of zinc binding domain revealed by structures of σ28-dependent transcribing complexesbioRxiv - Molecular Biology 2020Quote: ... lysed by ultrasonication and then centrifuged at 80,000 g for 1 h using a T29-8×50 fixed angle rotor (Sorvall LYNX 6000 superspeed centrifuge, Thermo Scientific). For σ28 purification ...
-
bioRxiv - Genetics 2019Quote: A set of 4x 40 µl of PCR reaction was mixed with 8 µl 6x DNA load dye (Thermo Scientific) and run on a 2% TBE agarose gel (UltraPure ...
-
bioRxiv - Genetics 2020Quote: ... samples were diluted in Laemmli sample buffer and 2 μg of protein was separated on a NuPage 3–8% Tris-Acetate gel (Invitrogen). The gel was then stained with Coomassie Blue and digitalized.
-
bioRxiv - Developmental Biology 2021Quote: ... were lysed in urea buffer (8 M urea, 100 mM NaHPO4, pH 8.5) with HALT protease and phosphatase inhibitor (Pierce/Thermo Scientific) and processed at the Emory Proteomics Core ...
-
bioRxiv - Microbiology 2021Quote: ... A 1ml-overlay of 1:1 mixture of 2X Minimal Essential Medium (MEM; Hyclone, SH30008.04) with 8% FBS and 1.5% low melting agarose (ThermoFisher Scientific, R0801) was placed in the wells ...
-
bioRxiv - Molecular Biology 2020Quote: ... 25 mg Orange G in 10 mL H2O) was added to each reaction and run on an 8% TBE gel (Invitrogen) (180 V ...
-
bioRxiv - Neuroscience 2020Quote: ... siRNAs and Gapmers or shRNAs were introduced to primary hippocampal neurons (5–8 days in vitro (DIV)) using Lipofectamine RNAiMAX or Lipofectamine 2000 (Invitrogen) according to manufacturer’s guidelines ...
-
bioRxiv - Bioengineering 2021Quote: ... IL-8 concentrations in medium samples from the basal compartment were measured using the IL-8 Human Uncoated ELISA Kit (88-8086-88, Invitrogen). Assays were performed in Nunc MaxiSorp (tm ...
-
bioRxiv - Bioengineering 2020Quote: ... The cells were passaged at a density of 1:2 to 1:8 after reaching ~80% confluency by detaching with 5 mM EDTA in PBS (Invitrogen 15575-038 diluted in sterile 1X PBS ...
-
bioRxiv - Biochemistry 2021Quote: ... Purified proteins were dialyzed in Spectra-Por7 semi-permeable dialysis membranes (8 kDa cut-off; Thermo Fisher Scientific, Waltham, MA) for 48 hours against conservation buffer (20 mM Tris pH=8.0 ...
-
bioRxiv - Biochemistry 2020Quote: Cells were trypsinized and seeded at 20,000 cells/well in an 8-well chambered coverglass (Nunc™, 12-565-470) with fresh DMEM (+ ...
-
bioRxiv - Neuroscience 2021Quote: ... Neurons were then transferred to pre-warmed neuronal media and plated at a density of 2,000 cells per well in an 8-well glass Lab-Tek slide (Thermo Scientific) pre-coated with poly-D-lysine (Corning ...
-
bioRxiv - Neuroscience 2021Quote: ... seeded at 1.5 x 105 neurons per coverslip in Neuron Medium (8 % FBS, 2 % B-27, [Gibco, 17504-044], Penicillin [200 U/mL]/Streptomycin [200 μg/mL] [Gibco, 15140-130] ...
-
bioRxiv - Neuroscience 2020Quote: A craniotomy was performed on 8-week-old mice and 200nl of a 10 μg/ml solution of Hoechst 33342 (ThermoFisher) was injected in multiple spots in 200 – 300 μm depth ...
-
bioRxiv - Molecular Biology 2019Quote: Alternative oxidase (AOX) cDNA from Pacific oyster (Crassostrea gigas) (Genbank, #ACL31211) (8) was cloned into the pcDNA6A mammalian expression vector (Invitrogen). First ...
-
bioRxiv - Neuroscience 2020Quote: ... Mφ were plated in an 8-well chamber slide (Nunc Lab-Tek II Chamber Slide System, ThermoFisher scientific, Massachusetts, USA) (50,000 cells/well) ...
-
bioRxiv - Genomics 2020Quote: ... The DNA was eluted in 150 μL 10 mM Tris-HCl pH 8 and the biotinylated molecules captured with Dynabeads M280 (Invitrogen) according to the manufacturer’s protocol but using150 μL Bead Binding buffer (Phase Genomics ...
-
bioRxiv - Bioengineering 2021Quote: ... was performed by incubating the tissue-attached HDMI-array in 40 μl RT reaction solution containing 8 μl Maxima 5x RT Buffer (EP0751, Thermofisher), 8 μl 20% Ficoll PM-400 (F4375-10G ...
-
bioRxiv - Bioengineering 2021Quote: ... cells were cultured on 8-well Nunc™ Lab-Tek™ II chambered coverglass (155409; Thermo Scientific™, Waltham, MA). For gene expression studies ...
-
bioRxiv - Cell Biology 2021Quote: ... dried peptide sample was resuspended in 200 mM HEPES (pH 8) and incubated for 1 h at room temperature with one of the TMT10-plex reagents (ThermoFisher) solubilized in 100% anhydrous ACN ...
-
bioRxiv - Plant Biology 2020Quote: ... five-day-old seedlings were transferred to new MS plates supplemented with or without 8 μM zeocin (Invitrogen Life Technologies). For aluminium treatments ...
-
bioRxiv - Plant Biology 2020Quote: ... five-day-old seedlings were transferred to new MS plates supplemented with or without 8 μM zeocin (Invitrogen Life Technologies). For aluminium treatments ...
-
bioRxiv - Microbiology 2021Quote: ... Transfection was performed on 8-well glass slides at 90% confluent cells using Lipofectamine® 3000 (Cat: L3000075, Invitrogen Co.), following the manufacturer’s instructions and a total of 250 ng of DNA per well was transfected ...
-
bioRxiv - Molecular Biology 2020Quote: ... lysed by ultrasonication and then centrifuged at 80,000 g for 1 h using a T29-8×50 fixed angle rotor (Sorvall LYNX 6000 superspeed centrifuge, Thermo Scientific). The recombinant proteins were purified through 5 mL HisTrap HP column (GE Healthcare) ...
-
bioRxiv - Microbiology 2021Quote: ... or 3.8 x 106/ml (Δfut1s) and grown for three days in the presence/absense of 100µM alkyne fucose (Invitrogen C10264), after which parasites were washed in PBS ...
-
bioRxiv - Biochemistry 2021Quote: ... An S2 cell pellet from a 5 mL culture was resuspended with 400 μL buffer A (50 mM Tris, pH 8, NaCl 150 mM, 20% glycerol and 1x protease inhibitor; Cat# A32965, ThermoFisher). Then the cell lysate was frozen rapidly in liquid nitrogen and thawed in room temperature water bath ...
-
bioRxiv - Cancer Biology 2020Quote: ... Primary murine hepatocytes were isolated from livers of WT and mTORLEC mice at 8-12 weeks of age and maintained in DMEM (Gibco) with 10% fetal bovine serum as previously reported (22) ...
-
bioRxiv - Biophysics 2021Quote: ... was cultured in Baby Hamster Kidney (BHK)-21 cells at 37°C for 8-10 h with Dulbecco’s modified Eagle’s medium (DMEM) (Gibco, CA, USA) supplemented with 100 U/ml penicillin ...
-
bioRxiv - Cancer Biology 2021Quote: MDA-MB-231 (2 x 103 cells/well) and MDA-MB-453 (8 x 103 cells/well) were seeded in 96-well plates (Nunc) and allowed to attach overnight ...
-
bioRxiv - Cell Biology 2020Quote: ... the GFP-B16 tumor spheroids were transferred to Lab-Tek II cover-glass-bottomed 8-chamber slides and imaged in HBSS supplemented with 2% FBS (all from Invitrogen) using a 63×/1.4 NA oil-immersion objective.
-
bioRxiv - Cell Biology 2021Quote: ... Protein samples were loaded for each group in each well and run on 8-16% sodium dodecyl sulfate–polyacrylamide gel (ThermoFisher) for electrophoresis and then transferred to the PVDF membrane ...
-
bioRxiv - Cell Biology 2022Quote: ... and 300 μl of resuspended cells in growth medium were transferred to a well of an 8-well chambered coverglass slide (Nunc). Cells were then imaged 48 hours after addition of growth medium using a Zeiss LSM 880 confocal microscope.
-
bioRxiv - Immunology 2022Quote: ... 8-16 barcoded samples were pooled and loaded on one Ion 530 chip using the Ion Chef Instrument (ThermoFisher Scientific) and sequenced on the Ion S5 System with 550 flows.
-
Dynamic chromatin organization and regulatory interactions in human endothelial cell differentiationbioRxiv - Developmental Biology 2022Quote: ... from the RUES2 line (RUESe002-A; WiCell) were maintained on recombinant human Laminin-521 matrix (rhLaminin521; Biolamina) in Essential 8 (E8) media (ThermoFisher) at a density of 0.5 µg/cm2 ...
-
bioRxiv - Microbiology 2022Quote: ... Transfection was performed on 8-well glass slides at 90% confluent cells using Lipofectamine® 3000 (Cat: L3000075, Invitrogen Co.), following the manufacturer’s instructions and 250 ng of plasmid DNA per well were transfected ...
-
bioRxiv - Neuroscience 2022Quote: SOSRS imaged before day 14 were grown on Nunc™ Lab-Tek™ III 8-well chamber slides (Thermo Scientific) and fixed in paraformaldehyde for 30 min at room temperature (RT) ...
-
bioRxiv - Neuroscience 2022Quote: Differentiated cells on day 15 (8 days suspension culture plus 7 days adherent culture) were harvested and dissociated using TrypLE Select (Gibco). Cells were then passed through a 20 µm strainer and resuspended in 1 x PBS with 0.04% BSA buffer ...