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Citations for Thermo Fisher :
2151 - 2200 of 10000+ citations for 5 Nitro 1H benzo de isoquinoline 1 3 2H dione since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2024Quote: ... cells were stained with 1/300 DRAQ5 (Thermo Fisher, stock 5 mM) and sorted using a BD Influx cell sorter to discard debris and dublets ...
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bioRxiv - Systems Biology 2020Quote: ... RNA was radioactively 5' end-labeled using 1 U μl−1 T4 polynucleotide kinase (Thermo Fisher Scientific) and 0.5 μCi μl−1 32P-γ-ATP (PerkinElmer ...
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bioRxiv - Developmental Biology 2023Quote: ... 1 μl 10 μM 5‘-biotinylated oligo-dT30VN (IDT) and 1 μl 10 mM dNTP (Thermo Scientific). Cells were sorted at one cell per well ...
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bioRxiv - Immunology 2021Quote: ... Human dermal fibroblasts were generated from explant cultures of de-epidermized dermis as described40 and grown up to passage 4 in DMEM/F-12 (Thermofisher) complemented with 10% FBS and pen/strep antibiotics ...
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bioRxiv - Immunology 2021Quote: ... The purity and concentration of the mRNA samples were examined using a NanoDrop 2000 spectrometer (Thermo Fisher Scientific, DE, USA) and 2% agarose electrophoresis ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... The concentration and quality of extracted RNA from different tissues were measured using NanoDrop® 1000 (Thermo Scientific, Wilmington, DE) and reverse-transcribed into cDNA using Taqman Reverse Transcription Reagents (Applied Biosystems ...
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bioRxiv - Neuroscience 2021Quote: ... concentration and purity of isolated samples were measured using a NanoDrop ND-1000 Spectrophotometer (Thermo Scientific, NanoDrop Products, Wilmington, DE). Using the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... RNA concentrations were attained by measuring absorbance at 260 nm using a NanoDrop 8000 spectrophotometer (Thermo Fisher Scientific, Wilmington, DE) and first-strand cDNA was synthesized from 1.5 µg total RNA using the high-capacity cDNA reverse transcription kit (Applied Biosystems ...
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bioRxiv - Microbiology 2021Quote: ... Concentration and quality of the DNA samples was measured by a NanoDrop ND-1000 spectrophotometer (Thermo Scientific, Wilmington, DE, USA). The four samples were combined and diluted to 20 ng/μl for final analysis ...
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bioRxiv - Cancer Biology 2021Quote: Human T cells from healthy de-identified donors were purchased from the University of Pennsylvania Immunology Core and stimulated with CD3/CD28 beads (11131D, Gibco) as previously described(46) ...
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bioRxiv - Molecular Biology 2022Quote: ... Samples were then de-crosslinked and RNA was degraded using 2 ul of 10mg/ml Ambion RNAse (Invitrogen, cat# AM2270) for 2 hrs ...
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bioRxiv - Genetics 2022Quote: ... and a Qubit 4 Fluorometer with the Quant-iT™ Qubit™ dsDNA BR Assay Kit (Thermo Scientific, Wilmington, DE). DNA concentrations can be found in Table S2.
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bioRxiv - Immunology 2022Quote: ... The monolayers were then overlaid with a 1:1 solution of 2.5% Avicel® RC-591 microcrystalline cellulose and carboxymethylcellulose sodium (DuPont Nutrition & Biosciences, Wilmington, DE) and 2X Modified Eagle Medium (Temin’s modification, Gibco™) supplemented with 2X antibiotic-antimycotic (Gibco™) ...
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bioRxiv - Developmental Biology 2020Quote: ... total RNA was extracted from biological triplicates of de-yolked CRISPRi-injected and dCas9-injected control embryos using trizol (Invitrogen) and purified using Direct-zol RNA Miniprep kit (Zymo) ...
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bioRxiv - Biochemistry 2020Quote: ... Injected peptides were on-line de-salted using a C18 PepMapTM 100 trap column (300um i.d. x 5mm, 5um, 100Å, Thermo Scientific) for 2 min using 2% MeCN + 0,1% FA ...
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bioRxiv - Zoology 2020Quote: ... Quality and quantity of purified RNA was checked by spectrophotometry using a NanoDrop 2000 (Thermo Fisher Scientific, Wilmington, DE, USA). The RNA integrity was confirmed with the Agilent TapeStation 4200 (Agilent Technologies ...
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bioRxiv - Genetics 2021Quote: ... Isolated DNA was quantified by ultraviolet absorbance spectroscopy (260 nm) using a NanoDrop spectrophotometer (Thermo Scientific/NanoDrop Products, Wilmington, DE).
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bioRxiv - Cell Biology 2020Quote: De novo protein synthesis was measured using Click-IT HPG Alexa Fluor 488 protein synthesis assay kit (Thermo Fisher Scientific) as per company instructions ...
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bioRxiv - Developmental Biology 2020Quote: ... mosquitoes were offered a sterile bloodmeal consisting of 50 % human red blood cells (Etablissement Français du Sang de Guadeloupe-Guyane) and 50 % foetal bovine serum (Thermo Fisher). The bloodmeal was provided to mosquitoes in an autoclaved Hemotek reservoir (Hemotek ...
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bioRxiv - Physiology 2021Quote: ... RNA concentration was quantified using Qubit RNA HS assay kits and a Qubit 2.0 Fluorometer (Thermo Fisher Scientific, Wilmington, DE). Total RNA was stored at -80°C ...
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bioRxiv - Molecular Biology 2021Quote: For Input DNA 100 µL of lysate was treated and de-crosslinked by incubation with proteinase K (Thermo-Fisher Scientific) for 4 h at 55°C and DNA was purified using DNA clean-up kit (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2021Quote: ... Genomic DNA purity was confirmed via an A260/A280 measurement (target ≥1.8) using NanoDrop™ One (Thermo Scientific™, DE), and DNA concentration was quantified using Qubit® 3.0 (Thermo Fisher Scientific Inc. ...
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bioRxiv - Molecular Biology 2023Quote: ... The concentration and quality of extracted RNA from the lung tissues were measured using NanoDrop One (Thermo Scientific, Wilmington, DE) and reversetranscribed into cDNA using either Taqman Reverse Transcription Reagents (Applied Biosystems ...
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bioRxiv - Genetics 2023Quote: ... The concentration and purity of the exosomal RNA samples were estimated using NanoDrop ND-1000 spectrophotometer (Thermo Scientific, Wilmington DE).
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bioRxiv - Neuroscience 2023Quote: ... The resulting de-stain was transferred to a new plate and absorbance at 595nm was measured using a Varioskan microplate reader (ThermoFisher). The resulting values were applied as a scaling factor in Seahorse Wave analyzer software (V2.6.3.5 ...
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bioRxiv - Microbiology 2022Quote: ... RNA concentration was determined using spectrophotometric optical density measurement (A260/A280) by NanoDrop™ One (Thermo Fisher Scientific, Wilmington, DE). For Q-PCR ...
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bioRxiv - Physiology 2024Quote: ... #CC-2561) and the Hospices Civils de Lyon and were cultured in growth medium consisting of DMEM/F12 (Gibco, 10565018), 20 % Fetal Bovine Serum (Gibco ...
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bioRxiv - Microbiology 2023Quote: ... The quality and quantity of the plasmid DNA were measured using a NanoDrop 2000 (Thermo Fisher Scientific, Wilmington, DE, USA) and a Microplate Reader SH-9000Lab (Corona Electric ...
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bioRxiv - Immunology 2024Quote: ... Supelco) in LC-MS grade water (Ultra CHROMASOLV, Honeywell Riedel-de Haën) with 0.1% ammonium hydroxide (Alfa Aesar, Thermo Scientific) and 5 µM InfinityLab deactivator additive (Agilent Technologies).
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bioRxiv - Microbiology 2024Quote: ... the DNA samples extracted from the endosphere samples were quantified using a NanoDrop 1000 Spectrophotometer (Thermo Scientific, Wilmington, DE, USA). Extracted DNA was then sequenced for 16S rDNA sequencing.
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bioRxiv - Microbiology 2024Quote: ... the quantity and quality of each DNA sample were assessed using a NanoDrop 1000 Spectrophotometer (Thermo Scientific, Wilmington, DE, USA). For DNA extraction from the inner part of plant roots (endosphere) ...
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bioRxiv - Developmental Biology 2022Quote: ... was de novo synthesized and inserted into the PacI/SacI restriction enzyme site pair of pMK-T (Thermo Fisher Scientific) by the manufacturer ...
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bioRxiv - Cancer Biology 2022Quote: ... Mass spectra were acquired using a Voyager DE-STR MALDI–time-of-flight mass spectrometer (software version 5.1; Applied Biosystems) in reflector mode ...
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bioRxiv - Molecular Biology 2023Quote: ... the cell extracts or de-crosslinked samples were subjected to SDS–PAGE separation on NuPAGE 4–12% gels (Invitrogen, NP0321) in MES buffer ...
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bioRxiv - Immunology 2024Quote: ... In brief, hiPSCs were differentiated to definitive endoderm (DE) using Activin A (100 ng/mL, R&D) in RPMI-1640 (Gibco) with increasing concentrations of HyClone-defined fetal bovine serum (HC FBS ...
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bioRxiv - Microbiology 2024Quote: ... the gel was de-stained in stain base solution overnight with rocking and then stained using Pierce Silver Stain Kit (ThermoFisher) and imaged on a Syngene G:box using the visible protein setting.
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bioRxiv - Microbiology 2024Quote: ... The purity and quality of the samples were assessed using a NanoDrop 2000 Spectrophotometer (Thermo Fisher Scientific, DE, United States). The microbial community was characterized using the 16S rRNA amplicon sequencing technique ...
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bioRxiv - Evolutionary Biology 2024Quote: ... grown to 70% confluency in a Dulbecco’s Modified Eagle Medium mixture (cytiva HyClone DMEM, Wilmington, DE, USA; 10% Fetal Bovine Serum, Fisher Scientific; 100 U/mL penicillin and streptomycin ...
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bioRxiv - Biophysics 2022Quote: ... and 2 ng/mL recombinant murine interleukin-3 (IL-3) (Gibco). Cells were grown in a humidified incubator at 37 °C and 6% atmospheric CO2 ...
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bioRxiv - Immunology 2022Quote: ... 1.9g of ethyl-3-(3-dimethylaminopropyl)-carbodiimide hydrochloride (EDC) (ThermoFisher #22980) and 1.2g of N-hydroxysuccinimide (NHS ...
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bioRxiv - Cell Biology 2024Quote: ... human custom-made 3’UTR HEC1 siRNA (oligo #3, Thermo Fisher Scientific ...
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bioRxiv - Biophysics 2024Quote: ... and 3-pyridinemethanol (RI = 1.545, 3-PM, A10381, Thermo Fisher Scientific) were used to mix with the water-based SMLM buffer ...
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bioRxiv - Microbiology 2020Quote: ... Four to six-day old mated female mosquitoes were allowed to feed for 1h on approximately 108 PFUs of SINV (TE12-untagged) containing citrated rabbit blood (Fisher Scientific DRB030) supplemented with 1mM ATP (VWR ...
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bioRxiv - Microbiology 2020Quote: ... 30,000 cells/well were cultured at 37°C in 5% CO2 as a monolayer on coverslips (d = 12 mm; Paul Marienfeld GmbH & Co. KG) in 24 well plates (#08-772-1H, Thermo Fisher Scientific) with DMEM ...
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bioRxiv - Microbiology 2021Quote: ... Cells infected with YFV were incubated with the pan-flavivirus anti-Env 4G2 antibody for 1h at 4°C and then with Alexa 488 anti-mouse IgG secondary antibodies (Thermo Fisher, #A28175) for 45 min at 4°C in the dark ...
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bioRxiv - Cancer Biology 2020Quote: ... the flasks were shaken at 37°C for 1h at 170 rpm in an orbital shaker (Max Q 4000; Thermo Fisher Scientific). The supernatant containing microglia was then collected [36].
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bioRxiv - Neuroscience 2020Quote: ... for 1h at room temperature (or overnight at 4°C) and the unbound dye was removed using spin columns (Thermofisher, Waltham, MA). The labeling efficiency was estimated using absorbance values (488 or 555 or 660 nm/280 nm ...
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bioRxiv - Microbiology 2022Quote: ... Bound primary antibody was then labeled for 1h at room temperature using Alexa488 goat anti-mouse IgG secondary antibody (Invitrogen, OR, USA) at a 1:750 dilution in PBS ...
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bioRxiv - Neuroscience 2022Quote: ... samples were washed three times and incubated 1h at room temperature with secondary antibodies: Alexa Fluor Plus 488 goat anti-Rabbit (A32731, Thermo Fisher Scientific) and Alexa Fluor Plus 546 donkey anti-goat (A11056 ...
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bioRxiv - Microbiology 2021Quote: ... The beta-propiolactone was inactivated by incubation at 37C for 2 hours and the inactivated virions were pelleted by ultracentrifugation at 25000g for 1h at 40C and protein concentration was determined by BCA assay (Thermo Fisher Scientific).