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Citations for Thermo Fisher :
1851 - 1900 of 10000+ citations for Anion Exchange Membranes Thickness 10 13 µm since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... Corning™ Accutase™ detachment solution and phorbol 12-myristate 13-acetate (PMA) were obtained from Fisher Scientific (cat ...
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bioRxiv - Cancer Biology 2020Quote: ... BT-12 and BT-13[7] were cultured in Dulbecco’s modified Eagle’s medium with Nutrient Mixture F-12 (DMEM/F12, Gibco) supplemented with 2 mM L-glutamine ...
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bioRxiv - Molecular Biology 2022Quote: ... INS-1 832/13 cells were cultured in 11 mM glucose RPMI 1640 (Thermo Fisher Scientific, Cat# 11875) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Evolutionary Biology 2022Quote: ... cDNA libraries were amplified using 12-13 PCR cycles and quantified on a Qubit Fluorometer (Thermo Fisher Scientific). Average fragment size was determined on a Fragment Analyzer (Agilent) ...
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bioRxiv - Genomics 2022Quote: ... and a 1:100 dilution of Anti-mouse CD34 biotinylated antibody (Thermo Fisher Scientific, Cat#: 13-0341-85) for 20min at 4°C ...
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bioRxiv - Developmental Biology 2021Quote: ... for ten minutes at room temperature in the cavity of a glass spot plate (Fisher Scientific 13-748B). They were then transferred to another cavity containing 2.5 % Liberase (5 g Liberase reconstituted in 2ml nuclease free water ...
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bioRxiv - Immunology 2022Quote: ... Human IFNg and IL-13 cytokine secretion was measured by ELISA using eBioscience Ready-SET-Go! (now ThermoFisher) ELISA kits according to the manufacturer’s protocol.
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bioRxiv - Immunology 2022Quote: ... 002-13 and 034-32 were collected on a 300 kV Titan Krios transmission electron microscope (ThermoFisher Scientific) equipped with Gatan K3 direct electron detector behind 30 eV slit width energy filter ...
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bioRxiv - Physiology 2022Quote: ... The samples were combined with 1 ml of 3:13 dilution of Erlich’s reagent [1.5 g of 4- (dimethylamino) benzaldehyde (ThermoFisher); 5 ml ethanol ...
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bioRxiv - Neuroscience 2024Quote: ... We dissected the larval whole brains (at 6 or 13 dpf) in the RNAlater® (AM7021, Ambion, USA) after overnight incubation and the adult brains (at 120 dpf ...
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bioRxiv - Bioengineering 2024Quote: ... the samples were exposed to the E-cadherin monoclonal primary antibody (HECD-1; 1:2000; 13-1700, Invitrogen) in PBST supplemented with 1% BSA for 24 hours at 4°C ...
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bioRxiv - Developmental Biology 2024Quote: ... cDNA conversion was performed using the high-capacity cDNA reverse transcription kit (Thermo Fisher, Cat. #43-668-13) according to manufacturer’s guidelines ...
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bioRxiv - Cell Biology 2023Quote: ... anti-mouse integrin β3 (armenian hamster monoclonal, 2C9.G3, Thermo Fisher Scientific, 13-0611-81; FC 1:200), anti-mouse integrin α5 (rat monoclonal ...
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bioRxiv - Immunology 2023Quote: Supernatants from restimulated mesenteric lymph node lymphocytes and tissue homogenates were assessed for IL-13(88-7137, Invitrogen), IL-17A (88-7371 ...
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bioRxiv - Genetics 2023Quote: ... The membrane was boiled for 30 min in water and incubated for 1 hour in 5% skim milk in TBS pH 7.4 then for 2 hours in primary antibody (1:2,000 ubiquitin monoclonal antibody 13-1600 Invitrogen) and for 1 hour at room temperature in secondary antibody (1:4,000 anti-Mouse-HRP ...
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bioRxiv - Molecular Biology 2023Quote: ... Cell lystaes were then transferred to low-protein binding Eppendorf tubes (Cat. No. 13-698-794, Fisher Scientific) containing 3 μl of anti-REV-ERBα antibody ...
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bioRxiv - Microbiology 2024Quote: ... aureus were cultivated at 37°C in meat peptone medium: nutrient broth 13 g/L (Thermo Fisher Scientific); yeast extract 3 g/L (Carl Roth) ...
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bioRxiv - Microbiology 2024Quote: ... The blot was stained with the primary mouse α-c-Myc Monoclonal 9E10 antibody (Invitrogen, catalog # 13-2500) and Goat anti-mouse IgG1 Cross-Adsorbed Horseradish Peroxidase secondary antibody (Invitrogen ...
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bioRxiv - Physiology 2024Quote: ... cells were rinsed with Tyrode’s buffer and incubated for 13 – 15 minutes in Tyrode’s buffer with 1 μM Fura-2 AM (ThermoFisher) at room temperature ...
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bioRxiv - Microbiology 2024Quote: ... THP-1 were differentiated by the addition of 150ng/mL phorbol 12-myristate 13-acetate (PMA) (ThermoFisher Scientific). The epithelial cell lines were cultured according to ATCC guidance for the specific cell line and as adherent cells ...
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bioRxiv - Microbiology 2024Quote: ... Corning™ Accutase™ detachment solution and phorbol 12-myristate 13-acetate (PMA) were obtained from Fisher Scientific (cat ...
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bioRxiv - Biochemistry 2024Quote: ... cells were seeded at low density onto 13 mm glass coverslips and propagated in 24-well dishes (Nunc) containing 1 ml growth medium ...
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RNA Binding of GAPDH Controls Transcript Stability and Protein Translation in Acute Myeloid LeukemiabioRxiv - Cancer Biology 2024Quote: Total RNA was isolated from 3-5 × 106 cultured MOLM-13 cells using TRIzol reagent (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... MOLM-13 and MV4-11 were cultured in Roswell Park Memorial Institute medium (RPMI-1640, Thermo Fisher Scientific) supplemented with 20% fetal bovine serum (FBS) ...
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bioRxiv - Cancer Biology 2021Quote: ... freshly isolated MNCs were washed and re-suspended in PBS and stained for 10 min at 37°C with 2’,7’-dichlorodihydrofluorescein diacetate (H2DCFDA) at a concentration of 5 µM (Thermo Fisher Scientific, Waltham, MA, USA). H2DCFDA is a non- fluorescent dye which is cleaved inside cells to 2’,7’-dichlorofluorescein (H2DCF) ...
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bioRxiv - Biochemistry 2024Quote: Full-length Mical1 (25 µM) was mixed with a 10-fold excess of freshly dissolved DSBU (disuccinimidyl dibutyric urea; Thermo Fisher SCIENTIFIC, 50 mM stock) in DMSO in a cross-linking buffer (20 mM Hepes ...
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bioRxiv - Genomics 2020Quote: ... PMPs were harvested from suspension during medium exchange and plated in microglia differentiation media over 7-14 days to produce microglia like cell monocultures (Neurobasal (Life Technologies 21103049) supplemented with Gem21 NeuroPlex without Vitamin A (GeminiBio ...
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bioRxiv - Biochemistry 2020Quote: ... Unbound nucleotides were removed after the reaction by buffer exchange into wash buffer using a Zeba spin desalting column (Thermo Fisher Scientific). Bound nucleotides were analyzed by HPLC as described above ...
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bioRxiv - Cell Biology 2021Quote: Vps22 was diluted to 15 μM after the buffer exchange to XL-buffer and incubated with 16 mM EDC (Thermo Scientific, #22980) and 16 mM Sulfo-NHS (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... a 75% media exchange (150uL) was performed in all wells with NB/B27+ media (B-27 Plus Neuronal system kit (Life technologies A3653401). On D5 ...
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bioRxiv - Synthetic Biology 2020Quote: Sample purity and oligomeric state was analyzed by online buffer exchange MS44 using a Vanquish UHPLC coupled to a Q Exactive Ultra-High Mass Range (UHMR) mass spectrometer (Thermo Fisher Scientific) 45,46 modified to allow for surface-induced dissociation (SID ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... After incubation unreacted antibiotics were removed by buffer exchange into 100 mM potassium phosphate buffer (pH7.4) using protein desalting spin columns (ThermoFisher part number 89849) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... They were positioned within slots imprinted from a custom-designed mold cast (NIH 3D Print Exchange, ID: 3DPX-021299) in 2% agarose (Fisher Scientific, BP160) dissolved in hypotonic (Π = 10 mOsm ...
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bioRxiv - Molecular Biology 2020Quote: ... followed by 0.45-μm membrane filtration and then 0.22-μm membrane filtration (ThermoFisher Scientific Ltd). The filtrate obtained from untreated SM was designated as SM_NT and the filtrate obtained from untreated IMF was designated as IMF_NT.
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bioRxiv - Biophysics 2021Quote: ... We experimentally determined the precise membrane position using DiD membrane staining dye (Thermo Fisher, D7757). We found that after thresholding the 488ex channel image by Li’s algorithm and then eroding the binary mask by 3 px ...
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bioRxiv - Microbiology 2020Quote: ... DNA was transferred to positively charged nylon membrane (BrightStar-Plus Positively Charged Nylon Membranes; Invitrogen) using a Semidry Electroblotting System (Thermo Scientific ...
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bioRxiv - Neuroscience 2020Quote: ... Membrane proteins were extracted using the Mem-PER Plus Membrane Protein Extraction Kit (89842, ThermoFisher) and separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE ...
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bioRxiv - Cell Biology 2022Quote: ... membranes were transferred to a nitrocellulose membrane via the iBlot™ Gel Transfer Device (Invitrogen). Membranes were blocked overnight with 3% BSA (ALB001.100 ...
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bioRxiv - Microbiology 2021Quote: ... the cells were resuspended in 30 μL PBST containing 3.3 μg/mL DAPI and 10 ng/μL membrane staining dye FM4-64X (Thermo Scientific, Waltham, MA, USA), and incubated for 10 min on ice in darkness ...
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bioRxiv - Neuroscience 2020Quote: ... all membranes were washed three times for 10 minutes in TBST and visualised with luminol reagents (Amresco, Ohio, USA or Thermo Scientific, Rockford, USA) by the ChemiDoc™ MP imaging system (Bio-Rad ...
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bioRxiv - Neuroscience 2024Quote: ... for each sample were loaded onto 10% polyacrylamide gels and separated by electrophoresis and transferred overnight to nitrocellulose membranes (Thermo Fisher, Stockholm, Sweden). The membranes were immunoblotted with primary antibodies against actin (1:30,000 ...
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bioRxiv - Molecular Biology 2023Quote: ... Membranes were washed with 1x TBST (4 x 10 min) and processed using SuperSignal West Pico PLUS Chemiluminescent Substrate (Thermo Fisher Scientific PI34080).
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bioRxiv - Immunology 2024Quote: Membrane and cytosolic fractions were generated from 10×106 U-937 cells using the Mem-PER™ Plus Membrane Protein Extraction Kit (#89842; Thermo Fisher Scientific). Incubation times were optimized to increase protein yield to 45 minutes for the permeabilization buffer and 90 minutes for the solubilization buffer ...
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bioRxiv - Neuroscience 2024Quote: ... or onto Amersham Hybond 0.2 um PVDF membranes (Cytiva 10-6000-22) at 80V for 120 min in a solution of Tris-Glycine Buffer (Thermo Fisher Scientific 28363). Membranes were blocked for 1 hr at room temperature in gentle shaking with blocking buffer (0.1% Casein [BioRad 1610782] in Tris Buffered Saline [TBS]) ...
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bioRxiv - Biochemistry 2024Quote: ... 0.3 mg of TEV protease was added to concentrated eluate to remove 6xHis tag and sample dialyzed using 10 kDa MWCO dialysis membrane (Thermo Scientific Cat# PI88243) in protein storage buffer (50 mM Tris ...
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bioRxiv - Developmental Biology 2024Quote: ... Around 20 µg of total protein was loaded onto 7.5-10% SDS-PAGE gels for separation and then transferred to PVDF membranes (ThermoFisher Scientific, #88518, Massachusetts, USA). The membranes were blocked with 15% skimmed milk in TBST for at least 3 hours at room temperature or overnight at 4°C ...
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bioRxiv - Immunology 2020Quote: ... onto an Acclaim PepMap RSLC analytical column (75 µm x 50 cm, nanoViper, C18, 2 µm, 100 Å; Thermo Scientific). The peptides were separated by increasing concentrations of 80 % ACN / 0.1 % FA at a flow of 250 nl/min for 65 min and analyzed with a QExactive Plus mass spectrometer (Thermo Scientific) ...
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bioRxiv - Biophysics 2020Quote: ... Analytes were separated on an Acclaim PepMap RSLC column (25 cm x 75 µm, 2 µm particle size, Thermo Scientific) over a 60-minute gradient from 7% to 35% acetonitrile at a flow rate of 300 nl/min ...
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bioRxiv - Cell Biology 2022Quote: ... the mixture was strained through 70 µm and 30 µm cell strainer and washed thoroughly by DMEM/F12 medium (Gibco) and centrifuged at 300 g for 10 min at 4°C ...
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bioRxiv - Immunology 2022Quote: ... Aag2 cells were treated with a final concentration of either 30 µM chlorpromazine or 15 µM nystatin (Thermo Fisher Scientific). Following a two-hour incubation ...