Labshake search
Citations for Thermo Fisher :
2051 - 2100 of 10000+ citations for Anion Exchange Membranes Thickness 10 13 µm since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Animal Behavior and Cognition 2021Quote: ... Coronal sections of 150□μm of thickness were mounted on gelatin-coated microscope slides with Permount Mounting Medium (Thermo Fisher Scientific) and dried at room temperature overnight ...
-
bioRxiv - Developmental Biology 2022Quote: ... Transverse cryosections were cut at 12-micron thickness and mounted onto poly-L-lysine pre-coated glass slides (Superfrost Plus; Thermo Fisher). For mCherry and ZPR2 staining ...
-
bioRxiv - Pathology 2021Quote: ... Each paraffin-embedded tissue was sequentially sectioned at 5 μm thickness into 5 consecutive sections and were mounted on HistoGrip (Invitrogen, US) coated glass slides ...
-
bioRxiv - Bioengineering 2021Quote: ... Cells were seeded at a density of 10,000 cells/cm2 onto fibronectin-coated glass coverslips (#CS-25R17, thickness 1.5, Warner Instruments, Hamden CT) (fibronectin, 20 μg/ml, #33016-015, Thermo Fisher Scientific) mounted in 6-well plates ...
-
bioRxiv - Biochemistry 2024Quote: 40 μm-thick cross sections prepared from both native and pretreated poplar samples were incubated in 0.05 M acetate buffer at pH 5 for 30 min prior to mounting in the same medium on a microscope slide covered with a coverslip (0.17 mm thickness) separated by a spacer (Gene frame ® 65 μL, Thermo Scientific) to provide a 65 μL sealed chamber containing 60 μL enzyme-buffer solution to avoid the evaporation during the enzymatic hydrolysis ...
-
bioRxiv - Neuroscience 2023Quote: ... Embryonic tissues were sectioned in either coronal or sagittal orientation at 20-30μm thickness and directly mounted onto superfrost glass slides (Thermo Fisher Scientific). Postnatal tissues were cryosectioned in coronal orientation at 30-45μm thickness and first collected in PBS and then mounted onto glass slides ...
-
bioRxiv - Neuroscience 2023Quote: ... Samples were then cryo-embedded in Optimal Cutting Temperature medium (Tissue-Tek, #4583) and sectioned at a thickness of 12- micrometers using the Cryostar NX70 (ThermoFisher Scientific) (7 ...
-
bioRxiv - Biophysics 2023Quote: ... To make the chambers 4 pairs of holes were drilled through a glass slide (Thermo Scientific 25*75 mm, 1mm thickness), which served as the inlet and outlet for sample injection ...
-
bioRxiv - Neuroscience 2023Quote: The brain was sectioned in the coronal plane at 40-μm thickness using a sliding microtome (HM 430; Thermo-Fisher Scientific), mounted on a slide glass ...
-
bioRxiv - Systems Biology 2024Quote: ... Fixed and frozen organoids were cryosectioned to 18µm thickness and mounted on SuperFrost Plus microscope slides (Fisher Scientific #12-550-15).
-
bioRxiv - Neuroscience 2024Quote: ... Cerebellar tissues were sagittally cryosectioned at a thickness of 35 μm and stored in cryopreserving buffer containing 30% ethylene glycol (Fisher) and 20% glycerol (Acros Organics) in PBS at −20°C until processed.
-
bioRxiv - Microbiology 2024Quote: ... Lamellas were milled to a nominal 200 nm thickness and stored in liquid nitrogen for less than a week before being loaded into a Titan Krios (ThermoFisher Scientific) for data collection.
-
bioRxiv - Plant Biology 2024Quote: ... Ultrathin sections of 50 nm thickness were analyzed using a Tecnai G2 Spirit transmission electron microscope (FEI, ThermoFisher Scientific, SNU CMCI) at 120 kV.
-
bioRxiv - Biochemistry 2024Quote: WT TF1: The cryo-EM samples were screened for ice thickness and particle density using a Talos Arctica (Thermo Fisher Scientific) transmission electron microscope (TEM) ...
-
bioRxiv - Developmental Biology 2021Quote: ... The following antibodies and dye were used in this study: anti-rat E-cadherin (Invitrogen, 13-1900, 1:300), anti-rabbit cleaved caspase-3 (Cell signaling ...
-
bioRxiv - Immunology 2021Quote: ... 13-0443-82) and 1:200 VE-cadherin-Alexa Fluor 647(rat anti-mouse, clone 11D4.1, Thermo Fisher, BDB562242) in 1.25% BSA in PBS for 24 hours ...
-
bioRxiv - Immunology 2021Quote: ... Samples were stained using 50μl of intracellular anti-cytokine antibody stain: PE-Cy7-conjugated anti-IL-13 (eBio13A, Invitrogen), PE-conjugated anti-IL-5 (TRFK5 ...
-
bioRxiv - Microbiology 2021Quote: ... a homolog of the vaccinia virus H3L gene) [13] using the Path-ID™ qPCR Master Mix (Thermofisher Scientific). Nucleic acid from capripoxvirus positive samples was further analysed by a qPCR targeting LSDV011 designed to differentiate the three capripoxvirus species [14] and a qPCR targeting LSDV008 designed to differentiate LSDV wildtype strains from Neethling vaccine strains [15] ...
-
bioRxiv - Microbiology 2021Quote: A549-ACE2 cells were reverse transfected using 20pmol of Non-targeting siRNA (D-001206-13-20) or IFITM2 siRNA (M-020103-02-0010) and 1μL of RNAi max (Invitrogen). Cells were incubated for 24h prior to a second round of reverse transfection ...
-
bioRxiv - Plant Biology 2020Quote: ... All conductivity measurements were performed with an Accumet 200 conductivity meter (Fisher Scientific, probe: catalog number 13-620-101). Initial readings were collected from samples incubated at 0°C for 30 min in a pre-cooled chiller (initial measurement) ...
-
bioRxiv - Cell Biology 2020Quote: ... referred to herein as INS-1 832/13) were cultured in RPMI 1640 medium with 11.1 mM D-glucose (Invitrogen) supplemented with 10% fetal bovine serum (FBS) ...
-
bioRxiv - Neuroscience 2021Quote: ... The neuronal cultures were then incubated for 13-20 DIV in Neurobasal™-A supplemented with B-27 (Invitrogen), 50 IU/ml penicillin and 50 μg/ml streptomycin at 37°C before experimental procedures ...
-
bioRxiv - Bioengineering 2022Quote: ... cDNA was synthesized using the Applied Biosystems™ High-Capacity cDNA Reverse Transcription Kit (Fisher Scientific, 43-688-13) according to manufacturer’s instructions ...
-
Biodiversity and Antimicrobial Potential of Bacterial Endophytes from Halophyte Salicornia brachiatabioRxiv - Microbiology 2020Quote: ... Fractions 11 and 13 containing bioactive compounds were further chromatographed on semi-preparative HPLC (Dionex Ultimate 3000, Thermo Scientific) using gradient mixtures of acetonitrile–water (4:1 to 9:1 ...
-
bioRxiv - Molecular Biology 2020Quote: ... was used to extract RNA and fragmented for 13 mins in 70°C using RNA Fragmentation Reagents (Life Technologies) and later purified by running through RNase-free P-30 column (Bio-Rad ...
-
bioRxiv - Cell Biology 2021Quote: ... first dimensional lanes are cut from the gel and incubated in 1% SDS with 1% beta-mercaptoethanol for 1 hour before the gel slice was loaded into an NuPAGE 4-13% 1.0mmx2D gel (Invitrogen). Gels were run at 200V for 50 minutes at room temperature in MOPS ...
-
bioRxiv - Physiology 2021Quote: The INS-1 832/13 cells (gift from C. Newgard PhD, Duke University) were cultured in RPMI-1640 (Gibco) supplemented with 10% FBS and 1% penicillin-streptomycin (Gibco ...
-
bioRxiv - Immunology 2021Quote: ... Cells were washed twice with 1X BD Perm/Wash Buffer (PWB) and stained with PE-eFluor610-IL-13 (Invitrogen), Brilliant Violet 421-IL-5 (Biolegend) ...
-
bioRxiv - Immunology 2020Quote: 1×105 A549-ACE2 cells were reverse transfected using 20pmol of Non-targeting siRNA(D-001206-13-20) or IFITM2 siRNA (M-020103-02-0010) and 1μL of RNAi max (Invitrogen). Cells were incubated for 24h prior a second round of reverse transfection ...
-
bioRxiv - Immunology 2022Quote: ... and cloned using SacI and ApaI in the pCR3 SARS-CoV-2-SΔ19 expression plasmid (13) using Gibson Assembly (ThermoFisher). All constructs were verified by Sanger sequencing ...
-
bioRxiv - Microbiology 2022Quote: A549-ACE2 cells were reverse transfected using 20pmol of Non-targeting siRNA (D-001206-13-20) or IFITM2 siRNA (M-020103-02-0010) with 1μL of RNAi max (Invitrogen). Cells were incubated for 24h prior to a second round of reverse transfection ...
-
bioRxiv - Neuroscience 2022Quote: ... The neuronal cultures were then incubated for 13-20 DIV in Neurobasal™-A supplemented with B-27 (Invitrogen), 50 IU/ml penicillin and 50 μg/ml streptomycin at 37°C before experimental procedures ...
-
bioRxiv - Cell Biology 2022Quote: ... RNA was reverse-transcribed to cDNA by High Capacity cDNA Reverse Transcription Kit (Thermo Fisher Scientific, 43-688-13). A SYBR Green-based master mix (Thermo Fisher Scientific ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... and water) and polytetrafluoroethylene (PTFE) syringe filters (0.45 μm, 30 mm; 0.22 μm, 13 mm) were purchased from Thermo Fisher Scientific (Waltham ...
-
bioRxiv - Cell Biology 2023Quote: ... RNA was converted into cDNA using the High Capacity cDNA Reverse Transcriptase kit (Thermo Fisher Scientific #43-688-13) according to manufacturer’s instructions ...
-
bioRxiv - Biophysics 2024Quote: ... The experimental and control devices were then placed inside an environmental chamber (Thermo Scientific, Catalog no. 13-067-066) and incubated at 30 ◦C and 50 % humidity ...
-
bioRxiv - Developmental Biology 2024Quote: Retinas were cultured on 13 mm/0.2 micron floating filters (Cytiva, 10417001) on DMEM/F-12 media (Gibco, 11320082) with 10% Fetal Bovine Serum (Thermofisher ...
-
bioRxiv - Bioengineering 2024Quote: ... and RNA quality/yield were then measured using a NanoDrop™ OneC spectrophotometer (Thermo Fisher Cat#13-400-519) prior to cDNA synthesis ...
-
bioRxiv - Neuroscience 2024Quote: ... fluorescence in situ hybridization (FISH; 13 mm cover glasses [631-1578; VWR] in 24 well plates [150628; Thermo Fisher]), immunofluorescence studies (µ-Slide 8 Well [80826 ...
-
bioRxiv - Genomics 2024Quote: ... Hand pumping was carried out with sterile 50 mL BD luer lock syringes (Fisher Scientific, 13-689-8 BD). 740 μl of Qiagen Buffer ATL was then added per filter and the filters stored at -20°C until transport to the lab (Trinity College Dublin ...
-
bioRxiv - Bioengineering 2024Quote: ... diluted in a weight-ratio of 13:1:1:1:2 in 1.5 mL of Opti-MEM (31985062, Gibco). This solution was added to the 10 cm dish in a drop-wise pattern when the cells had reached ∼60% confluency ...
-
bioRxiv - Cell Biology 2024Quote: ... cells were incubated overnight at 4°C in 1X PBS containing MAP2 monoclonal antibody (1:300; ThermoFisher, #13-1500). The next day ...
-
bioRxiv - Microbiology 2021Quote: ... Membranes were stained for total protein using the Novex™ Reversible Membrane Protein Stain Kit (Invitrogen) following the manufacturer’s instructions ...
-
bioRxiv - Immunology 2020Quote: ... Fixed cells were washed twice with PBS and stained for membranes with DiD membrane dye (Invitrogen), Alexa Fluor™ 594 Phalloidin (Invitrogen ...
-
bioRxiv - Microbiology 2024Quote: ... To transfer the binding reactions to the nylon membrane (Biodyne™ B Nylon Membrane, Thermo Scientific), we semi-dry transferred the reactions at 15 V for 45 min and immediately crosslinked the transferred DNA to the membrane using a commercial UV-light crosslinking oven (UV Stratalinker ...
-
bioRxiv - Neuroscience 2023Quote: ... We prepared membrane protein samples using the Mem-Per Plus Membrane Extraction Kit (Thermo Fisher #89842) with small adjustments to the protocol ...
-
bioRxiv - Cell Biology 2024Quote: Plasma membrane lipid labeling was carried out using CellMask Deep Red Plasma membrane stain (Invitrogen, #C10046) with 1:1000 dye dilution in normal culture media ...
-
bioRxiv - Cancer Biology 2024Quote: ... an additional 150 µL of a 1:1 mixture of NK-Xpander complete medium:OncoPro complete medium containing 500 U/mL rhIL-2 and 10 µM Invitrogen™ CellEvent™ Caspase-3/7 Red Detection Reagent (Thermo Fisher Scientific, cat# C10430) was added to each well ...
-
bioRxiv - Neuroscience 2020Quote: ... the C2 domains mutated fragments carrying aspartate/alanine exchange (C2A*: D225, 227, 233A; C2B*: D357, D359A) (Bacaj et al., 2013) were synthesised (Invitrogen GeneArt Gene Synthesis) and subcloned into the Syt-7 WT construct ...
-
bioRxiv - Molecular Biology 2020Quote: An untargeted analysis workflow was applied using a reagent-free ion exchange chromatography system with eluent generation (RFIC-EG) hyphenated with a high-field Orbitrap platform (Thermo Scientific, Bremen, Germany). The autosampler and columns were thermostated at 8 °C and 40°C ...