Labshake search
Citations for Thermo Fisher :
1801 - 1850 of 10000+ citations for Anion Exchange Membranes Thickness 10 13 µm since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biochemistry 2021Quote: ... and re-suspended in 200 µl M9 containing 1.5 µM of fluorescently-labelled colicin and 20 µM Hoechst 33342 (Thermofisher). Cells were incubated for 30 minutes at room temperature with rotary inversion in the dark ...
-
bioRxiv - Microbiology 2021Quote: ... An Acclaim PepMap 100 C18 trap column (75 µm x 2.0 cm, 3 µm, 100 Å, Thermo Scientific, USA) and an Acclaim PepMap RSLC C18 nanoLC column (75 µm x 25 cm ...
-
bioRxiv - Microbiology 2020Quote: ... onto an Acclaim PepMap RSLC analytical column (75 µm × 50 cm, nanoViper, C18, 2 µm, 100 Å; Thermo Scientific). The peptides were separated by increasing concentrations of buffer B (80% acetonitrile / 0.1% formic acid ...
-
bioRxiv - Cell Biology 2021Quote: ... Pellets were discarded and the supernatants were filtered through 0.45 µm and 0.22 µm mesh (Fisher Scientific, Leicestershire, UK) to eliminate dead cells and debris ...
-
bioRxiv - Biochemistry 2022Quote: ... The eluate was loaded onto a reversed-phase analytical column (50 µm × 250 mm, 2 µm, C18, Thermo Fisher) with an elution gradient of 4%-35% mobile phase B (80% acetonitrile + 0.1% formic acid + 20% water at a flow rate of 300 nL/min ...
-
bioRxiv - Neuroscience 2022Quote: ... onto an Acclaim PepMap RSLC analytical column (75 µm x 50 cm, nanoViper, C18, 2 µm, 100å; Thermo Scientific). The peptides were separated by increasing concentrations of 80% ACN / 0.1% FA at a flow of 250 nl/min for 158 min and analyzed with a QExactive HF mass spectrometer (Thermo Scientific ...
-
bioRxiv - Pathology 2021Quote: ... and separated on an analytical column (PepMap RSLC C18, 75 µm x 50 cm, 2 µm particles, Thermo Scientific) at 250 nL/min with a 160-min gradient of 3-25% of solvent B followed by 10-min increase to 40% and 5-min increase to 85% ...
-
bioRxiv - Biochemistry 2022Quote: ... onto an Acclaim PepMap RSLC analytical column (75 µm x 50 cm, nanoViper, C18, 2 µm, 100å; Thermo Scientific). The tryptic peptides were separated by increasing concentrations of 80% ACN / 0.1% FA at a flow of 250 nl/min for 128 min and analyzed either with an Orbitrap Fusion Tribrid or QExactive Plus mass spectrometer (Thermo Fisher Scientific ...
-
bioRxiv - Microbiology 2020Quote: ... and a 75 µm x 15 cm analytical column with C18 2 µm resins (Acclaim PepMap RSLC, Thermo Scientific). The nanoLC gradient was 3-35% solvent B (A = H2O with 0.1% formic acid ...
-
bioRxiv - Cancer Biology 2021Quote: ... and an Acclaim PepMap 100 trap column (100 µm x 2 cm, nanoViper, C18, 5 µm, 100Å; Thermo Scientific), the tryptic peptides were separated by increasing concentrations of 80% acetonitrile (ACN ...
-
bioRxiv - Cancer Biology 2021Quote: ... and an Acclaim PepMap 100 trap column (100 µm x 2 cm, nanoViper, C18, 5 µm, 100Å; Thermo Scientific), the tryptic peptides were separated by increasing concentrations of 80% ACN/0.1% formic acid at a flow of 250 nL/min for 90 min and analyzed with a QExactive HF mass spectrometer (Thermo Scientific) ...
-
bioRxiv - Cell Biology 2023Quote: ... using a nano-LC column (Acclaim PepMapC100 C18, 75 µm x 50 cm, 2 µm, 100 Å, Thermo Scientific). In general ...
-
bioRxiv - Microbiology 2023Quote: ... Cultures were continued for another 3.5 h prior to addition of 1 µM NAO and/or 1 µM ReAsH-EDT2 reagent (Invitrogen). Cultures were incubated for 1 h at room temperature and washed with 1X BAL wash buffer per manufacturer kit instructions ...
-
bioRxiv - Biochemistry 2024Quote: ... and target defocus values from -1.0 to -2.4 µM in 0.2 µM intervals in an automatic way using EPU software (Thermo Fisher Scientific). For the acquisition of the JSR1-jsGiq complex ...
-
bioRxiv - Cell Biology 2024Quote: ... equipped with a trapping column (3 µm C18 particle, 2 cm length, 75 µm ID, Acclaim PepMap, Thermo Scientific) and a 50 cm long separation column (2 µm C18 particle ...
-
bioRxiv - Neuroscience 2023Quote: ... Electroporation solutions were filtered with 0.45 µm and 0.20 µm filters (Nalgene Syringe Filters, Cellulose Acetate, 4 mm, Thermo Scientific) before loading into the glass pipettes ...
-
bioRxiv - Microbiology 2023Quote: ... with an Acclaim PepMap RSLC analytical column (75 µm x 50 cm, nanoViper, C18, 2 µm, 100Å; Thermo Scientific) and an Acclaim PepMap 100 trap column (100 µm x 2 cm ...
-
bioRxiv - Microbiology 2023Quote: ... and an Acclaim PepMap 100 trap column (100 µm x 2 cm, nanoViper, C18, 5 µm, 100Å; Thermo Scientific) using a 120 minute linear gradient for separation with two FAIMS compensation voltages (-45 ...
-
bioRxiv - Molecular Biology 2023Quote: ... and an Acclaim PepMap 100 trap column (100 µm x 2 cm, nanoViper, C18, 5 µm, 100Å; Thermo Scientific), the tryptic peptides were separated by increasing concentrations of 80% acetonitrile (ACN ...
-
bioRxiv - Cell Biology 2024Quote: ... peptides were resolved using an EASY-Spray capillary HPLC column (ES902A, 75 µm × 25 µm, 100Å, 2μm, Thermo Scientific) and a Vanquish Neo UHPLC (Thermo Fisher Scientific ...
-
bioRxiv - Systems Biology 2024Quote: ... Peptides were separated on a 75 µm × 50 cm EASY-Spray C18 column (2 µm, 100 Å; Thermo Scientific) at 50 °C using a linear gradient from 10% to 40% B in 153 min with a flow rate of 200 nL/min ...
-
bioRxiv - Cell Biology 2024Quote: ... with an EASY-Spray™ capillary HPLC column (75 µm × 25 cm, 100Å, 2 µm; ES902A, Thermo Fisher Scientific). The mass spectrometer was operated in data-dependent acquisition (DDA ...
-
bioRxiv - Biophysics 2024Quote: ... a 2 µM solution of F-actin was briefly incubated with 0.5 µM of Alexa488 Phalloidin (Thermo Fisher, A12379). The combined solution was gently pipetted with blunt-cut 200 μL pipette tips and immediately flowed into the chamber ...
-
bioRxiv - Synthetic Biology 2024Quote: ... and separation on a 3rd generation Aurora column (Ion Opticks, C18 1.7 µm, 250 mm x 75 µm) using a Vanquish Neo HPLC (ThermoFisher) and the following gradient of eluents A(0.1% formic acid in H2O ...
-
bioRxiv - Bioengineering 2024Quote: ... peptides were first trapped on a trap column (PepMap 100 µm × 2 cm, 5 µm particles, Thermo Fisher Scientific) using a flow rate of 5 µL min-1 and 0.1% formic acid ...
-
bioRxiv - Biochemistry 2020Quote: ... The grids were first screened for ice thickness and particle distribution using a Talos Arctica transmission electron microscope (ThermoFisher Scientific), operated at 200 keV and equipped with a K3 direct electron detector (Gatan) ...
-
bioRxiv - Neuroscience 2020Quote: ... coronal sections of BNST (12 μm thickness) were obtained and directly mounted onto Superfrost Plus slides (Fisher Scientific, Hampton, NH), then kept at −80° C ...
-
bioRxiv - Physiology 2021Quote: Coronal brain sections (10μm thickness) of each experimental group were immune-stained with Iba-1 (WAKO, Japan) and CD68 (Invitrogen, Sweden). The total number of Iba-1+ cells was counted in two regions of the hippocampus (dentate gyrus ...
-
bioRxiv - Developmental Biology 2022Quote: ... The 6 μm thickness section was prepared on the Microm HM 340 E rotary microtome (Thermo Scientific, Waltham, MA, USA), and dewaxed in xylene and serial concentrations of ethanol before staining ...
-
bioRxiv - Microbiology 2020Quote: ... Cells were grown until 70% confluency before transfection in 8 well Nunc Lab Tek II chambered slides with 0.16 mm cover glass thickness from Thermo Fisher Scientific (Waltham ...
-
bioRxiv - Neuroscience 2021Quote: ... Tissue samples were frozen in TissueTek and sectioned at 35 μm thickness on a cryostat (Thermo Scientific Microm HM 550) at −20°C ...
-
bioRxiv - Neuroscience 2021Quote: ... the transfected cells were plated on poly-L-lysine-coated 35-mm round coverslip (#1.5 thickness) (Fisher Scientific, Waltham, MA). The cells were used for TIRF imaging 2 days after transfection ...
-
bioRxiv - Neuroscience 2024Quote: ... Coronal brain sections measuring 16 μm in thickness were cut using a cryostat (Epredia Cryostar NX50, Fisher Scientific, Waltham, MA). The sectioning process was done in intervals of 0.5 mm throughout the brain ...
-
bioRxiv - Molecular Biology 2024Quote: ... Longitudinal sections were cut with a Leica VT1200S vibratome to a thickness of 100μm and incubated in CAS-block solution (008120, Thermo Fisher) for 30 minutes (diluted 1:10 in distilled water) ...
-
bioRxiv - Microbiology 2024Quote: ... dropped on a Superfrost Plus precleaned glass slide (Fisher 1255015), and then immediately covered with a Gold Seal cover glass (24×60 mm, 0.13 mm thickness) (ThermoFisher Scientific). The samples were kept in the dark and at room temperature for 20 minutes before imaging ...
-
bioRxiv - Biophysics 2023Quote: ... milling and polishing down to a target thickness of 200 nm was done in AutoTEM Cryo 2.4 (Thermo Fisher Scientific) reducing the ion beam current stepwise from 1 nA to 30 pA ...
-
bioRxiv - Cell Biology 2023Quote: ... Grids were stored in liquid nitrogen and screened for ice thickness and protein concentration using a ThermoFisher Glacios TEM equipped with a Falcon III (ThermoFisher).
-
bioRxiv - Neuroscience 2023Quote: ... Retinal and photoreceptor layer thickness were measured on cryosections prepared as described below and stained with Hoechst 33342 (1:10,000, Thermo Fisher) also using Fiji/ImageJ.
-
bioRxiv - Developmental Biology 2022Quote: ... the spinal cords were cryosectioned at 16 μm thickness and alternatingly placed on Superfrost microscope glass slides (Thermo Fisher Scientific) and Visium spatial gene expression slides (10x Genomics) ...
-
bioRxiv - Bioengineering 2023Quote: ... the samples were embedded in paraffin and sections of 4.5 μm thickness were cut with a microtome (Microm, HM430, Thermo Scientific). The sections were stained with Hematoxylin-Eosin (#GHS116 and #HT110116 ...
-
bioRxiv - Cell Biology 2022Quote: ... Tissue was washed three times in PBS and mounted on a glass slide in ∼50 µl of Vectashield and covered with a #1.5 thickness 22 x 40 mm cover glass (Cat# CLS-1764-2240, Fisher Scientific).
-
bioRxiv - Immunology 2023Quote: ... Cryosections of 14 μm thickness were generated using a cryostat (Microm Cryo-Star HM560, Thermo Fisher Scientific, Waltham, MA, USA). The sections were fixed for 1 hour with 4% paraformaldehyde (PFA ...
-
bioRxiv - Neuroscience 2024Quote: ... embedded in paraffin and cut at a thickness of 5µm on a Microm Microtome (Thermo Fisher Scientific Inc., Waltham, MA) and stained for routine neuropathological staging according to the NIA-AA Revised Criteria for Diagnosis and Staging of Alzheimer’s Disease [29].
-
bioRxiv - Genomics 2024Quote: Tissues were cut to ≤0.5 cm thickness and immediately placed in 5x volume RNAlater (Invitrogen Cat# AM7020, Waltham, MA, USA). After 5 minutes room temperature incubation ...
-
bioRxiv - Neuroscience 2024Quote: ... Slices were covered with a 22 mm x 50 mm 1.5 thickness coverslip using ProLong Glass Antifade Mountant media (Invitrogen, #P36984) and sealed with transparent nail polish ...
-
bioRxiv - Neuroscience 2024Quote: ... Coronal sections of 30 m thickness were obtained using a cryostat (CM1950; Lecia Biosystmes) and placed directly onto Superfrost slides (ThermoFisher). Sections were washed 3 times with 0.1% Triton-X-100 in PBS (PBST ...
-
bioRxiv - Microbiology 2024Quote: ... immobilized on #1.5 coverslips of 0.17 ± 0.02 mm thickness (Warner Instruments, Hamden, CT) previously coated with 0.1 mg/ml poly-D-lysine (Thermo Fisher Scientific) following the manufacturer’s recommendations ...
-
bioRxiv - Immunology 2021Quote: ... then reverse transcribed into cDNA using a high-capacity cDNA reverse transcription kit (Thermo Fisher, 43-688-13). RNA levels were quantified using the IDT Prime Time Gene Expression Master Mix ...
-
bioRxiv - Neuroscience 2021Quote: ... The custom heat block was attached to a temperature-controlled recirculating water bath (13-874-180, Fisher Scientific) set to the cultivation temperature ...
-
bioRxiv - Neuroscience 2021Quote: ... Then sections were incubated in primary antibody solution (GFAP, 1:1000, Life technologies, 13-0300, Rat monoclonal antibody) overnight at 4 °C ...