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Citations for Thermo Fisher :
1751 - 1800 of 10000+ citations for Anion Exchange Membranes Thickness 10 13 µm since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2024Quote: ... 100 µM 2-Mercaptoethanol (Gibco 31350-010),10 ng/mL Leukemia Inhibitory Factor (LIF ...
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bioRxiv - Cell Biology 2024Quote: ... supplemented with either 42 µM gentamicin (Gibco) or 1% penicillin/streptomycin (Gibco ...
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bioRxiv - Cell Biology 2021Quote: ... Samples eluted with glycine were subjected to separation with SCX (strong-cation exchange) column (Sepax Tech #Z777148) followed by digestion with trypsin (Gibco #R001100). SDS-Page gel samples were run against an empty vector control (pCMV empty ...
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bioRxiv - Microbiology 2021Quote: ... half of the lysate/VPM or lysate/PBS mixtures were subjected to a buffer exchange using a 100kDa MWCO protein concentrator (Pierce, Thermo Scientific), reduction to 20% volume and two washes with PBS prior to serial dilution and inoculation of Vero E6 cells as described above.
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bioRxiv - Cancer Biology 2021Quote: ... To visualize sister chromatid exchanges (SCE) slides were rehydrated in PBS for 5 min and stained with 2 μg/mL Hoechst 33342 (Thermo Fisher) in 2xSSC (final concentration 300 mM NaCl ...
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bioRxiv - Physiology 2024Quote: ... were used to perform buffer exchange (Phosphate-buffered saline [PBS] [Gibco, #14190], 50 mM NaCl [Sigma-Aldrich, #S5150-1L], 0.001 %, Pluronic F68 [v/v] [Gibco, #24040]) and the final concentration step ...
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bioRxiv - Cancer Biology 2023Quote: ... purified antibodies were run through a buffer exchange protocol using 50kDa ultra filtration columns (Amicon) and then partially reduced with 4mM TCEP (Thermo Scientific). Polymers were loaded individually with isotopically enriched metals ...
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bioRxiv - Cell Biology 2022Quote: ... The Strep (II) and Ni-NTA elution fractions were further purified via ion exchange chromatography (Poros HQ 50 μm, Life Technologies) and subjected to size-exclusion chromatography in a buffer containing 50 mM HEPES pH 7.4 ...
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bioRxiv - Cancer Biology 2020Quote: Equal volumes of eluate (25 μl) were separated by 10 % SDS-PAGE and transferred to nitrocellulose membranes (Invitrogen by Thermo Fisher Scientific). The membrane was stained with Ponceau S Stain (Boston BioProducts ...
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bioRxiv - Cancer Biology 2020Quote: Equal volumes of eluate (25 μl) were separated by 10 % SDS-PAGE and transferred to nitrocellulose membranes (Invitrogen by Thermo Fisher Scientific). The membrane was stained with Ponceau S Stain (Boston BioProducts ...
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bioRxiv - Physiology 2021Quote: ... proteins were detected by incubating the membranes with a polyclonal anti-mouse horseradish peroxidase (HRP)-conjugated secondary (1:10 000, 31430; Thermo Fisher Scientific), polyclonal anti-rabbit HRP-conjugated secondary (1:20 000 ...
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bioRxiv - Biophysics 2021Quote: ... Freshly isolated neutrophils (5×105 cells) were stained with 10 μg/ml of CellMask Deep Red plasma membrane stain (#C10046, Invitrogen, Carlsbad, CA), followed by co-infection with GFP-S ...
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bioRxiv - Physiology 2020Quote: Protein extracts from pancreas homogenates and acini normalized for protein concentration (10 μg protein) were subjected to SDS–PAGE and then transferred onto PVDF (Polyvinylidene fluoride) or nitrocellulose membranes (Thermo Fisher Scientific) for immunoblotting ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Equal protein concentrations were run on a 10% SDS-PAGE (polyacrylamide gel electrophoresis) gel and transferred to a polyvinylidene difluoride membrane (Thermo Fisher Scientific). Membranes were exposed to anti-p65 ...
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bioRxiv - Pathology 2021Quote: ... gels were incubated in 10% ethanol for dehydration before blotting the proteins to a membrane using the BlotTM 2 Gel Transfer Device (Thermo Fisher Inc.). After staining the membranes in Ponceau solution ...
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bioRxiv - Neuroscience 2020Quote: ... Samples were transferred onto an Invitrolon PVDF membrane activated with 10 % methanol using the iBlot 2 Dry Blotting System (Thermo Fisher Scientific). The membranes were then incubated in blocking buffer (1×TBS supplemented with 4.5 g/L Fish gelatin ...
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bioRxiv - Cancer Biology 2022Quote: ... 10 μM of Rock Inhibitor (Y-27632) on Geltrex LDEV-Free Reduced Growth Factor Basement Membrane Matrix (Cat# A1413202, Thermo Fisher Scientific). 48 hours later (D5) ...
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bioRxiv - Cell Biology 2023Quote: ... on tissue-culture dishes coated with 10 µg/cm2 of reduced Growth Factor Basement Membrane matrix (Geltrex, ThermoFisher A1413202 resuspended in basal medium DMEM/F12 Thermo Fisher 21331020). Medium was changed daily.
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bioRxiv - Microbiology 2023Quote: ... The membrane was then incubated in 10 ml Blotto + goat α-rabbit poly-horse-radish peroxidase secondary antibody (Invitrogen; 1:10,000 dilution) for 1-2 h at RT ...
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bioRxiv - Microbiology 2022Quote: ... HBLAK cells grown on membranes were incubated with the following primary antibodies: rabbit anti-TLR-2 polyclonal antibody (10 µg/ml, PA5-20020, Thermo Fisher Scientific), rabbit anti-TLR-4 polyclonal antibody (5 µg/ml ...
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bioRxiv - Cancer Biology 2024Quote: For flow cytometry analysis of mitochondrial membrane potential (MMP) and Mitochondrial mass T cells were stained with 10 nM MitoTracker Deep Red (ThermoFisher Scientific, M22426) and 10 nM MitoTracker Green (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: Cell lysates were boiled run on SDS-PAGE gels (10% acrylamide) and subsequently transferred to a nitrocellulose membrane using an iBLOT 2 Gel Transfer Device (ThermoFisher Scientific, IB21001). Nitrocellulose membranes were blocked for 1 h with 5 % dried milk resuspended in TBS-Tween 1 % ...
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bioRxiv - Genomics 2023Quote: ... lysates and immunoprecipitates were separated on a Bolt 10% Bis-Tris Plus gel and transferred onto a PVDF membrane (both Thermo Fisher Scientific). To determine total EPHB4 amounts and EPHB4 phosphorylation status the following antibodies were used ...
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bioRxiv - Cancer Biology 2023Quote: ... Membrane was subsequently washed 3×10 mins by PBST and developed using SuperSignal® West Dura Extended Duration Substrate (Thermo Scientific, 34094) and imaged by Gel imaging system (GE ...
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bioRxiv - Neuroscience 2024Quote: ... Brain extracts containing 60 ug of protein were loaded and separated on 6-10% denaturing polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to polyvinylidene difluoride (PVDF) membrane (Cat#88518; Thermo Fisher Scientific). The membrane was blocked with 5% milk in Tris-buffered saline with 0.1% Tween (TBST ...
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bioRxiv - Immunology 2024Quote: ... Membranes were washed three times with TBS (incubated 5-10 min per wash) then imaged using an iBright FL100 (Thermo Fisher Scientific) with the auto exposure setting.
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bioRxiv - Bioengineering 2024Quote: ... the HUVECs were incubated for 10 min with CellMaskTM Deep Red Plasma Membrane Stain (4 µg/mL final concentration in CLINIcell, C10046, Thermo Fisher Scientific) to stain the cell membrane ...
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bioRxiv - Cell Biology 2024Quote: ... Sch9) or 10% (Ypk1, Akt) SDS-page gels and blotted onto nitrocellulose membranes using the iBlot2 Gel Transfer System (Thermo Fisher Scientific). Membranes were blocked with BSA ...
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bioRxiv - Neuroscience 2020Quote: ... and transferred to membranes using the iBlot nitrocellulose membrane Blotting system (Thermo Fisher Scientific) by following manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2020Quote: Labelling of plasma membrane was achieved with CellMask™ Orange plasma membrane stain (ThermoFisher) solution ...
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bioRxiv - Cell Biology 2024Quote: ... Plasma membrane lipids were labeled using CellMask Deep Red Plasma membrane stain (Invitrogen, # C10046) at 10 µg/ml concentration ...
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bioRxiv - Cancer Biology 2021Quote: ... cell membrane marker (W6748, Invitrogen) for 10 min on ice ...
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bioRxiv - Bioengineering 2021Quote: ... Membranes were stripped (21059, ThermoFisher) for 30 min ...
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bioRxiv - Cell Biology 2021Quote: ... electrotransferred to nitrocellulose membranes (Invitrogen) and blocked by 5% powdered milk in PBSt (PBS supplemented with 0.1% tween ...
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bioRxiv - Microbiology 2021Quote: ... A PVDF membrane (ThermoFisher Scientific) with 0.45 µm pore size was activated by shortly immersing it in methanol ...
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bioRxiv - Neuroscience 2022Quote: ... transferred to PVDF membrane (Invitrogen), and followed by immunoblotting for Flag (1:1000 Mouse Anti-Flag M2 monoclonal ...
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bioRxiv - Biochemistry 2021Quote: ... with PVDF membrane (Invitrogen IB24002) for 7 min at 20V ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... and PVDF membranes (Life Technologies). The membranes were probed with mouse anti-human CFTR monoclonal antibodies n°596 or n°660 (Cystic Fibrosis Foundation ...
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bioRxiv - Cancer Biology 2023Quote: ... to nitrocellulose membranes (Thermo Scientific). The following primary antibodies were used ...
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bioRxiv - Neuroscience 2022Quote: ... to PVDF membranes (Invitrogen, IB242002) at 23V for 3 minutes ...
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bioRxiv - Molecular Biology 2024Quote: ... blotted to nitrocellulose membranes (Invitrogen), blocked with 5% nonfat milk and incubated with primary antibodies overnight ...
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bioRxiv - Molecular Biology 2023Quote: ... 3500 membrane (Thermo fisher 66330) against the dialysis buffer (20 mM HEPES pH 7.9 ...
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bioRxiv - Neuroscience 2022Quote: ... positively charged Nylon membrane (Invitrogen). The m6A antibody (SySy ...
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bioRxiv - Cell Biology 2022Quote: ... PIP Strips Membranes (Thermo Fisher) were blocked in 3% milk in TBST for 1 h ...
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bioRxiv - Immunology 2022Quote: ... transferred onto PVDF membrane (Invitrogen), and immunoblotted ...
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bioRxiv - Neuroscience 2024Quote: ... transferred to nitrocellulose membranes (Invitrogen) and probed with antibodies ...
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bioRxiv - Molecular Biology 2024Quote: ... and membrane depolarization (B34950, ThermoFisher) study ...
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bioRxiv - Molecular Biology 2020Quote: ... Peptides were separated using an analytical C18 Acclaim PepMap column (75 µm × 250 mm, 2 µm vesicles; Thermo Scientific) and a 180-min gradient at a flow rate of 300 µl/min ...
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bioRxiv - Genomics 2021Quote: ... filtered through a 35 µm strainer and stained with 3x staining buffer (12 µM DAPI, 0.4 U/µl Ambion RNase Inhibitor ...
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bioRxiv - Bioengineering 2021Quote: ... cells were live-dead stained with 2 µM calcein acetoxymethyl and 4 µM ethidium homodimer-1 (#L3224, Thermo Fisher) and imaged using fluorescent microscopy to compare cell density and viability between each silicone substrate ...