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Citations for Thermo Fisher :
1851 - 1900 of 8052 citations for 7 oxo 7 phenyl heptanoic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... FACS analysis of transfected BEAS-2B cells for caspase 3 & caspase 7 along with SYTOX AADvancedTM Dead Cell Stain was performed per manufacturer’s protocol (Thermo Scientific).
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bioRxiv - Plant Biology 2021Quote: ... primed reverse transcription of first-strand cDNA synthesis was carried out with 7 mg total RNA using Super ScriptTM III (Invitrogen). Equal volumes of each first-strand reaction were amplified with gene-specific primer pairs ...
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bioRxiv - Biochemistry 2021Quote: 3 biological replicates of wild type and 2 different clones METTL5-KO HeLa cells (clone 1 and 7) were grown to confluency prior to lysis in Trizol (Invitrogen). Total RNA was purified as described above (‘RNA purification’ ...
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bioRxiv - Physiology 2021Quote: ... JC-1 dye was added 10 min prior to recording (ex.488/em.535 and em.590) while CellEvent Caspase 3/7 (ThermoFisher) was added immediately prior to recording (ex.495/em.540).
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bioRxiv - Physiology 2020Quote: ... and incubated with 100 mM 6-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (6-NBDG) (Life Technologies) in 10 nM Tris/HEPES buffer containing 150 mM KCl or 150 mM NaCl for 30 minutes at 37 °C ...
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bioRxiv - Microbiology 2019Quote: ... was used to quantify the relative levels of cells using a Viia™7 Real-Time PCR Instrument (Applied Biosystems) with 50 ng gDNA template using the following primers ...
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bioRxiv - Biochemistry 2021Quote: ... Proteins were transferred onto a nitrocellulose membrane and analyzed by western blotting using an anti-His tag antibody (7) or stained with Krypton fluorescent dye (ThermoFisher) according to a company’s instruction ...
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bioRxiv - Biochemistry 2021Quote: ... with a 10-fold excess of N,N’-dimethyl-N-(iodoacetyl)-N’-(7-nitrobenz-2-oxa-1,3-diazol-4-yl) ethylenediamine (IANBD-amide, Molecular Probes). After 90 min on ice ...
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bioRxiv - Immunology 2021Quote: ... cells were incubated in glucose-free media containing 5 μg/ml 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (2-NBDG, Thermo Fisher) and 2.5% FBS at 37 °C ...
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bioRxiv - Bioengineering 2021Quote: Spleens and injection dLNs were collected on day 28 (7 days post-boost) and stored in ice-cold IMDM (Gibco) until further steps ...
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bioRxiv - Immunology 2021Quote: ... and then transferred to nitrocellulose membrane for 7 min at 20 V using the iBlot 2 Dry Blotting System (Invitrogen). Where protein phosphorylation status was investigated ...
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bioRxiv - Neuroscience 2020Quote: ... Plasmids were transfected into cultured neurons at DIV 7-9 using a calcium phosphate transfection kit (Thermo Fisher, K2780-01). Experiments were performed 2 or 3 days post transfection.
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bioRxiv - Neuroscience 2021Quote: Astrocytes at 3 DIV or at 7-8 DIV after lentivirus infection were loaded with 1 μg/ml Fura-2-AM (#F1221, ThermoFisher) in astrocyte culture medium for 30 min at 37 °C ...
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bioRxiv - Bioengineering 2021Quote: ... Culture medium from day 0 until day 7 was RPMI1640 (HyClone, SH30027.01) plus B-27 serum-free supplement without insulin (Gibco, A1895601). From day 7 and onwards RPMI1640 with B-27 serum free supplement with insulin (Gibco ...
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bioRxiv - Pathology 2021Quote: ... 35 mins in MES buffer for aSyn detection) and transferred to nitrocellulose membranes (0.2 μm) via Iblot (7 mins, 20 mV; Fisher Scientific). Initial blots were tested for optimisations of aSyn retention as labelled by MJFR1 either by pre-blocking heat treatment (microwaved ...
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bioRxiv - Immunology 2021Quote: ... 1 μg/ml -CD28 and 1:50 diluted conditioned supernatant from COS-7 cells transfected with a pcDNA1 vector (Invitrogen) encoding for recombinant chicken IL-2 (a kind gift from prof ...
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bioRxiv - Microbiology 2021Quote: ... cDNA was generated with 7 µl of resuspended RNA using SuperScript™ III First-strand synthesis system (Thermo Fisher Scientific), and primers A1 and A2 (Table 1 ...
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bioRxiv - Immunology 2020Quote: Cell survival was determined by analyzing forward/sideward scatter properties of cells and simultaneous staining with either Propidium iodide (PI), or 7-amino-Actinomycin D (7AAD eFluor670 dye, and LIVE/DEAD fixable violet stain (ThermoFisher), following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... Quantification of these genes was performed using SYBR Green on a ViiA™ 7 sequence detection system (Applied Biosystems, USA) with two replications for each sample ...
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bioRxiv - Immunology 2021Quote: ... NKp46+ and stained as indicated by manufacturer’s protocol with Cell Event Caspase 3/7 Green Flow Cytometry Assay Kit (Molecular Probes). All samples were analyzed using BD Canto FACS instrument ((BD Biosciences ...
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bioRxiv - Immunology 2020Quote: ... 2019-nCoV CDC RUO Kit (Integrated DNA Technologies) and QuantStudio™ 7 Flex Real-Time PCR System (Applied Biosystems™). PCR amplicons were quantified against in vitro transcribed RNA N gene fragment standard ...
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bioRxiv - Immunology 2020Quote: PBMC were seeded at a concentration of 7-8 × 106 cells/mL in RPMI 1640 medium without phenol red and glutamine (Gibco) supplemented with 10% heat-inactivated FBS (Gibco ...
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bioRxiv - Genomics 2021Quote: ... Apoptosis staining was performed using the CellEvent Caspase-3/7 Green Flow Cytometry Assay Kit (Thermo Fisher Scientific, Waltham, MA). Stained cell suspensions were measured with the BD LSRFortessa Cell Analyzer (BD Biosciences ...
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bioRxiv - Physiology 2020Quote: ... 1 mM fluorescent glucose analog 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (2-NBDG, Life Technologies) was diluted in Live Cell Imaging Solution (Invitrogen ...
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bioRxiv - Cell Biology 2022Quote: ... reverse transcription and second strand synthesis performed on the slide with cDNA quantification undertaken with qRT-PCR using KAPA SYBR FAST-qPCR kit (KAPA systems) and analysed on the QuantStudio 7-Flex system (ThermoFisher). FFPE samples were cut at the standardised 5 µm thickness onto four 6.5mm x 6.5mm capture areas ...
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bioRxiv - Immunology 2022Quote: ... The amplification reaction was monitored on the ViiA 7 Real-Time PCR System running on the QuantStudio Real Time PCR Software V1.3 (Thermo Fisher). The size of the respective amplicons was confirmed by 2% agarose gel and ethidium bromide staining ...
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bioRxiv - Cancer Biology 2022Quote: ... MCF-7 human breast cancer cells were obtained from ATCC and cultured in Roswell Park Memorial Institute medium (RPMI-1640; Gibco) supplemented with penicillin/streptomycin and 10% FCS ...
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bioRxiv - Biophysics 2022Quote: ... HEK293 and COS-7 cells were maintained at 37 ºC and 5% CO2 and cultured in Dulbecco’s modified Eagle media (Life Technologies) supplemented with 10% FBS (Hyclone ...
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bioRxiv - Cell Biology 2022Quote: Human breast cancer (MCF-7) cells were obtained from ATCC and cultured every two days in Minimum Essential Medium (MEM) Alpha media (Gibco) supplemented with 1 mM sodium pyruvate ...
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bioRxiv - Cell Biology 2022Quote: Lipid overlay assay was performed using PIP strips (nitrocellulose membrane spotted with 8 phosphoinositides and 7 other biologically relevant lipids) according to the manufacturer’s instructions (Thermo Scientific). Briefly ...
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bioRxiv - Immunology 2022Quote: ... Treg cells were stimulated for 7-8 days with Dynabeads™ Human T-Activator CD3/CD28 (1:1 beads:Treg cell ratio, Gibco), and cultured in X-VIVO 15 with Gentamicin ...
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bioRxiv - Genetics 2022Quote: ... The medium was changed to 500 μl EDM media 7 days later in 24-well flat-bottom plates for further differentiation (Nunc). After 4-7 days of culturing ...
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bioRxiv - Immunology 2022Quote: ... Bone marrow-derived macrophages (BMDMs) and dendritic cells (BMDCs) were generated for 7 days in respectively IMDM or RPMI-1640 medium (Gibco), supplemented with glutamine ...
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bioRxiv - Immunology 2022Quote: ... 5-7 × 106 cells were resuspended in 3 mL of FACS buffer (4% FBS in phosphate buffered saline (PBS, Gibco)) and sorted at the University of Massachusetts Amherst Flow Cytometry Core Facility using a BD FACSAria Fusion (Becton Dickinson) ...
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bioRxiv - Plant Biology 2022Quote: ... Entry clones were then introduced into the destination vector pH-Ubi-Cas9-7 which also encodes the Cas9-enzyme (Miao et al., 2013) using LR recombination (Invitrogen). T1 CRISPR/Cas9 lines were genotyped for the absence of the T-DNA construct by using the primers priMR410/411 (Hyg-1F/Hyg-1R) ...
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bioRxiv - Microbiology 2022Quote: ... TcdB protein was diluted to 0.05 µg/µL using phosphate buffer (100 mM KPO4, 150 mM NaCl, pH 7) containing 5X SYPRO Orange (Invitrogen S6650), and a serial dilution of test compound ...
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SLC15A4 favors inflammasome function via mTORC1 signaling and autophagy restraint in dendritic cellsbioRxiv - Immunology 2022Quote: Bone marrow cells were isolated and cultured for 7-9 d in RPMI-1640 medium (Gibco, ThermoFisher Scientific, Waltham, MA) supplemented with 10% low endotoxin-FBS (Hyclone ...
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SLC15A4 favors inflammasome function via mTORC1 signaling and autophagy restraint in dendritic cellsbioRxiv - Immunology 2022Quote: Bone marrow cells were isolated and cultured for 7-9 d in RPMI-1640 medium (Gibco, ThermoFisher Scientific, Waltham, MA) supplemented with 10% low endotoxin-FBS (Hyclone ...
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bioRxiv - Microbiology 2022Quote: ... CellEvent™ Caspase-3/7 Green Detection Reagent (C10723) and PrestoBlue™ Cell Viability Reagent (A13261) were purchased from ThermoFisher Scientific ...
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bioRxiv - Microbiology 2022Quote: ... using either a QuantStudio™ 7 Flex Real-Time PCR System or a StepOnePlus Real-Time PCR System (Applied Biosystems). Individual standard curves were generated specifically for each system ...
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bioRxiv - Microbiology 2022Quote: ... Sydney Strain SS1 (cagA+, vacA s2m2) were grown on brain heart infusion (BHI) agar (Difco, USA) containing 7% heat-inactivated horse serum (Invitrogen), antibiotics and IsoVitaleX as mentioned previously (33) ...
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bioRxiv - Microbiology 2022Quote: ... The loaded plate was amplified for 40 cycles via the ViiA 7 real-time PCR system (Thermo Fisher Scientific, Waltham). For each biological replicates (n = 3) ...
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bioRxiv - Molecular Biology 2023Quote: ... Northern blot analysis of U1 snRNA was performed using 5’-radiolabeled DNA probes (Sequence is listed in Supplemental Table 7) in ultrasensitive hybridization Buffer (Thermofisher). The radioactivity signals were analyzed by PhosphorImager (Typhoon FLA 7000).
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bioRxiv - Plant Biology 2023Quote: ... These cassettes were sub-cloned into a modified binary cas9 vector pH-Ubi-cas9-7 (Fayos et al., 2020; Miao et al., 2013) in a Gateway LR reaction (Invitrogen) to generate p-OsCPR5.1-A and p-OsCPR5.1-C constructs (Fig ...
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bioRxiv - Neuroscience 2022Quote: Larval fish (4-7 dpf) were mounted in a small drop of 2% low melting point agarose (ThermoFisher Scientific 16520) in the center of the uncoated side of a mirror galvanometer (Thorlabs GVS0111) ...
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bioRxiv - Physiology 2023Quote: ... at 1,000g for 2min and then immediately loaded into the QuantStudio 6 and 7 Flex real-time PCR system (ThermoFisher Scientific). A two-step cycling protocol was implemented to collect cycle threshold (Ct ...
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bioRxiv - Molecular Biology 2022Quote: ... Samples were resolved by 7% SDS-PAGE and transferred to nitrocellulose membrane by semi-dry i-blot transfer system (IB1001, Invitrogen). Inputs and elution fractions were analyzed on separate gels ...
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bioRxiv - Molecular Biology 2022Quote: ... Samples were resolved by 7% SDS-PAGE and transferred to nitrocellulose membrane by semi-dry i-blot transfer system (IB1001, Invitrogen). Inputs and elution fractions were analyzed on separate gels ...
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bioRxiv - Cancer Biology 2022Quote: The COS-7 cells were seeded on gold-nanoparticle-coated cover glasses and cultured overnight in DMEM (11965-092, Gibco) containing 10% FBS (10082147 ...
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bioRxiv - Synthetic Biology 2022Quote: ... 5’ and 3’ RACE ready cDNA was generated from RNA extracted from ~30 pairs of ovaries or testes dissected from 5-7 days-post-emergence (dpe) Liverpool strain adults using Trizol (Invitrogen). Primers LA1076 then nested with LA1352 (5’ ...