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Citations for Thermo Fisher :
1801 - 1850 of 10000+ citations for Urea Nitrogen BUN Detection Kit 10 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2022Quote: ... HEK293T cells were plated in 5mL DMEM supplemented with 10% FBS and 1% PSG in one well of a 6-well plate (Fisher Scientific) for each validation ...
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bioRxiv - Microbiology 2023Quote: ... OD600 was adjusted to 1 and 2.5 μl were mixed with 500 μl of RPMI + 1% CA + 10 μM EDDHA per well (final OD600 of 0.005) in a 48 well microtiter plate (Nunc, Thermo Scientific) or 0.5 μl were mixed with 100 μl RPMI + 1% CA + 10 μM EDDHA per well in a 96 well microtiter plate (Falcon flat bottom ...
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bioRxiv - Genomics 2023Quote: ... representative brain sections were wasted three times in 1× PBS and incubated in a 24 well plate at room temperature for one hour in blocking buffer: 10% goat serum (Gibco, 16210064), 1% BSA (Sigma-Aldrich ...
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bioRxiv - Microbiology 2023Quote: ... and 1 µL of template DNA (5-10 ng) were run on 96-well plates on a StepOne Plus Real-Time PCR system (ThermoFisher Scientific). A melting curve analysis was carried out to confirm the amplification of a single product in each reaction ...
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bioRxiv - Cell Biology 2023Quote: ... qPCR was performed using 2x qPCR BIO SyGreen Blue Mix Lo-ROX (PCR Biosystems) in triplicate 10 µL reactions in 384-well plates (Applied Biosystems). Amplification was performed on a QuantStudio™5 Real-Time PCR System (ThermoFisher Scientific ...
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bioRxiv - Biochemistry 2023Quote: ... PNKP expressing stable cell lines were transfected with 10 μM PNKP 3’-UTR specific siRNA (Horizon Discovery) in a 24-well plate using RNAiMAX (ThermoFisher Scientific) according to the manufacturer’s protocol to deplete endogenous PNKP ...
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bioRxiv - Microbiology 2023Quote: ... were infected with 10 × 2-fold serial dilutions of an equal volume of each RVP stock in 384-well plates (Cat# 164688, ThermoFisher Scientific) and incubated at 37°C ...
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bioRxiv - Neuroscience 2023Quote: ... qRT-PCR was conducted with cDNA in duplicate 10-μL reactions on a 384-well plate using the Maxim a SYBR Green/ROX qPCR Master Mix (2×) (Thermo Scientific). The reactions were incubated at 50°C for 2 min and then at 95°C for 10 min ...
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bioRxiv - Developmental Biology 2022Quote: ... was then transferred to a fresh six-well plate with 3mL of DMEM + 10% BCS + 1% penicillin/streptomycin (Gibco: 15070-063). Two recombinations were performed ...
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bioRxiv - Immunology 2022Quote: ... were coated with 50 μl (or 10 μl for 384- well plates) per well of a 2μg/ml Neutravidin (Life Technologies, 31000) solution in PBS ...
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bioRxiv - Immunology 2022Quote: ... plates were developed by the addition of 50 μl (or 10 μl for 384-well plates) per well of the HRP substrate TMB (ThermoFisher, 34021) for 10 min ...
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bioRxiv - Cell Biology 2023Quote: ... transfected cells are cultured for 3 days in a 6-well plate before they are gently detached with 10-min incubation in an enzyme-free cell dissociation buffer (Gibco, 13151014). These detached cells are then replated on ECM-coated substrates and imaged after 6 hrs.
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bioRxiv - Cell Biology 2023Quote: ... Cells were seeded onto poly-L-lysine (PLL)-coated 12-well plates in DMEM supplemented with 10% FBS and transfected using the RNAiMAX reagent (Life Technologies) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: Antigen protein (100 µL; 10 µg/mL) in carbonic buffer (50 mM; pH 9.5) was immobilized on Maxisorp 96-well plates (Thermo Fisher Scientific) and incubated at 4 °C overnight ...
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bioRxiv - Bioengineering 2023Quote: ... 2 x 107 HEK293T cells were seeded onto 15-cm plates in DMEM supplemented with 10% (v/v) FBS and 1% (v/v) antibiotic-antimycotic (ThermoFisher Scientific). At 16 hr after seeding ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were plated at a density of 1.5 × 10^5 per well on 6-well plates in Neurobasal media (Gibco 21103-049) containing B27 supplement (Gibco 17504-044) ...
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bioRxiv - Molecular Biology 2023Quote: ... 3 wells of a 6-well plate of mESC (corresponding to 6*10^6 cells approximately) were resuspended in 1 mL Trizol (Invitrogen,15596018) vortexed two times for 30 seconds and incubated 5 minutes at room temperature ...
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bioRxiv - Genetics 2024Quote: ... using 10 ng of cDNA and 250 nM of each primer on a MicroAmp 384-well reaction plate (Applied Biosystems #4309849). Relative transcription levels were calculated by normalizing to the geometric mean of ACTB and GAPDH cycle threshold values.
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bioRxiv - Cell Biology 2024Quote: ... the membranes were seeded with 10 μl of 1×106 cells/ml in a Falcon 24-well cell culture plate (Cat# 353047, Fisher Scientific) and the plate was incubated in the cell-culture incubator (37 °C and 8% CO2 ...
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bioRxiv - Plant Biology 2024Quote: ... 9-day-old seedlings were transferred onto 1% plant agar MS medium plates containing different concentrations of zeocin (0 μM, 10 μM, 50 μM, 170 μM) (Gibco, 10072492). Seedling roots were cut with a razor blade and collected after overnight zeocin exposure ...
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bioRxiv - Cell Biology 2024Quote: ... The cell suspension was diluted 10-fold with EMM2 liquid medium without thiamine in a 96-well DeepWellTM plate (ThermoFisher, #260251), and this was subjected to incubation at 33°C for 6 h with shaking at 900 rpm ...
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bioRxiv - Neuroscience 2024Quote: ... RT-qPCR reactions were carried out with 5 µL template volumes in a final reaction volume of 10 µL volumes in MicroAmp 96-Well Fast Reaction Plates (ThermoFisher, #4366932). Each target or multiplex combination was prepared with TaqPath 1-Step RT-qPCR Master Mix (ThermoFisher ...
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bioRxiv - Microbiology 2024Quote: ... OD600 was adjusted to 1 and 2.5 μl were mixed with 500 μl of RPMI + 1% CA + 10 μM EDDHA per well (final OD600 of 0.005) in a 48 well microtiter plate (Nunc, Thermo Scientific). 200 ng/ml aerobactin (EMC Microcollections GmbH) ...
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bioRxiv - Cancer Biology 2024Quote: ... PEG-4000 at concentrations ranging from 10 to 100 µM were transferred to a 96 well optical black/clear bottom plate (Thermo Scientific) and pre-incubated for 3 min at 4°C ...
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bioRxiv - Cell Biology 2024Quote: ... Transfections were performed with 10 nM of siRNA sequences (6-well plates) by using the Lipofectamine RNAiMax system (Thermo Fisher Scientific). Hypoxia was performed as previously described (24) ...
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bioRxiv - Physiology 2019Quote: ... Hearts were immersed for one day in a chemical mixture obtained by mixing 25 wt% urea (U16–3, Fisher Scientific Hampton, NH, USA), 25 wt% N,N,N0,N0-tetrakis(2-hydroxypropyl ...
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bioRxiv - Molecular Biology 2021Quote: Fifteen milligrams of powdered human liver tissue were lysed in 200 uL of lysis buffer (8M Urea, 50 mM Tris-HCl pH7.5, 150 mM NaCl and 1X Halt™ Protease Inhibitor Cocktail (Thermo Fisher Scientific, #87786)) ...
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bioRxiv - Microbiology 2020Quote: ... aliquots were diluted 1:2 and 1:5 in 2M urea solvent and their total protein concentration was measured using a Pierce Bicinchoninic Acid Protein Assay (Thermo Scientific, Waltham, MA) following manufacturer’s instructions ...
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bioRxiv - Systems Biology 2019Quote: ... Cells were washed twice in 1 × PBS and lysed in 8 M urea supplemented with 1 × Halt Protease Inhibitor Cocktail (Thermo Fisher Scientific, USA). Insoluble cellular material was removed by centrifugation at 18,000 ×g for 20 min at 4°C ...
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bioRxiv - Physiology 2019Quote: ... Tissue was homogenized with sterile plastic pestle in 100 μl lysis buffer (50 mM NH4HCO3, 6M urea solution) and sonicated with Sonic Dismembrator (Fisher Scientific, Model 120) at 50% amplitude for ten seconds ...
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bioRxiv - Molecular Biology 2019Quote: ... RNA samples were heated at 80°C for 2 min and 40-50 mg of RNA were loaded onto 15% TBE-Urea polyacrylamide gels (Invitrogen; Waltham, MA, USA) in 1x TBE (Ambion ...
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bioRxiv - Molecular Biology 2020Quote: ... The reaction was incubated 2h at 37°C and then loaded on a 15% acrylamide/8M urea precast gel (Invitrogen, cat n° EC6885BOX). The ligated RNA was purified through gel extraction ...
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bioRxiv - Biochemistry 2022Quote: ... and after adding extraction buffer (500 μL of 8M urea, 100 mM Tris, pH 8 and protease inhibitor cocktail; ThermoFisher Scientific; Cat# A32965) were sonicated at 30% amplitude with 3 cycles of 10 second pulses ...
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bioRxiv - Genomics 2024Quote: ... 100 ng of each probe set was then analyzed on a 12.5% Urea-PAGE gel stained with SYBR-Gold (Thermo Fisher Scientific, Waltham, MA).
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bioRxiv - Cell Biology 2024Quote: ... Gel slices of RNA fragments with sizes between 26-34 nt were excised from a 15% polyacrylamide TBE-Urea gel (Thermo Fisher Scientific, EC68855BOX). Eluted RNA was treated with T4 PNK and preadenylated linker was ligated to the 3’ end using T4 RNA ligase 2 truncated KQ (NEB ...
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bioRxiv - Biophysics 2022Quote: ... plate reader using 96-well NUNC optical bottom plates (Thermo Scientific). The total sample volume used was 100 μL for all the measurements ...
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bioRxiv - Bioengineering 2019Quote: Plate sorting was conducted using 96-well optical plates (Fisher Scientific) or qPCR plates (Biorad ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The plate was sealed with a plate film (Thermo Fisher 4311971) and centrifuged for 5 minutes at 500 x g at room temperature ...
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bioRxiv - Bioengineering 2021Quote: 96-well ELISA plates (Nunc MaxiSorp flat-bottom plates, Thermo Fisher) were coated with 10 nM RBD ...
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bioRxiv - Bioengineering 2023Quote: ... ELISA plates (MaxiSorp 96 well plates, Thermo Fisher #12-565-135) were coated overnight at room temperature with influenza A/Puerto Rico/8/34 virus particles (400 HAU/ml ...
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bioRxiv - Synthetic Biology 2023Quote: ... The plate was covered with an adhesive plate seal (Thermo Scientific) and loaded measured on a VARIOSKAN LUX (Thermo Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... The plate was sealed with a plate sealing film (Thermo Scientific) and incubated at 42 °C in a BMG FLUOstar Omega plate reader for 100 h with cycles of 1 min shaking (400 rpm double orbital ...
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bioRxiv - Plant Biology 2024Quote: ... the plates were sealed with Adhesive PCR plate foil (ThermoFisher AB0626) and immediately frozen on dry ice ...
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bioRxiv - Cancer Biology 2024Quote: Ninety-six well plates (Nunc MaxiSorp Flat-Bottom Plate, 44240421, Invitrogen) were coated with NeutrAvidin (2μg/ml ...
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bioRxiv - Immunology 2023Quote: ... plates were washed 5X with 0.05% Tween in 1X PBS and incubated with detection antibodies diluted in 1X Elispot diluent: anti-mouse GM-CSF Detection (Invitrogen, #88-7334-88, 1:250), anti-mouse IL-10 detection (Invitrogen ...
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bioRxiv - Molecular Biology 2021Quote: ... 10% acetic acid and 0.5% Coomassie G-250) and de-stained (10% methanol; 10% acetic acid) or silver stained using the Silver Quest kit (Thermo Fisher Scientific).
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bioRxiv - Genetics 2022Quote: ... CRISPR/Cas9 Ribonucleoproteins were formed by complexing 240ng sgRNA duplex with 1250ng Alt-R V3 Cas9 Protein (IDT) in 10 μL buffer R (Neon™ Transfection System 10 μL Kit, ThermoFisher Scientific) - a 1:1 molar ratio - for 10 minutes at room temperature (RT) ...
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bioRxiv - Synthetic Biology 2019Quote: ... and the supernatant was taken to measure the amount of secreted IL-10 using the IL-10 Rat ELISA Kit (Invitrogen, catalog # BMS629). The resulting values were normalized based on the cell viability ...
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bioRxiv - Molecular Biology 2020Quote: ... three individual ribodepletion on 10 μg of RNA each in a final volume of 10 μl were performed using the RiboMinus Plant kit (Thermo Fisher scientific) following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... Cells in a total culture volume of 10 ml were transfected with 10 µg of plasmid DNA using the ExpiFectamineTM 293 Transfection Kit (Thermo Fisher Scientific) according to the manufacturer’s instructions ...