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Citations for Thermo Fisher :
1851 - 1900 of 10000+ citations for Urea Nitrogen BUN Detection Kit 10 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... pH 6.5 or pH 7.5 and containing 10 µM nigericin and 10 µM valinomycin according to the manufacturer’s protocol (Intracellular pH Calibration Buffer Kit, ThermoFisher, Cat. No. P35379). The average of two technical replicates per calibration sample was plotted on a graph ...
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bioRxiv - Immunology 2021Quote: Supernatants from MSC and T-cell cocultures were analyzed using IL-10 Ready SET-Go! ELISA kits kits (Affymetrix, eBioscience) for secretion of IL-10 as previously described [31] ...
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bioRxiv - Cell Biology 2019Quote: ... All cells were authenticated by STR profiling (GenePrint 10 System kit from Promega and AuthentiFiler PCR Amplification Kit from ThermoFisher) and tested negative for mycoplasma contamination using the Cell Culture Contamination Detection Kit (ThermoFisher).
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bioRxiv - Developmental Biology 2019Quote: BeWO cell culture was harvested 48-hours post transfection media was subjected to lactate dehydrogenase (LDH) detection using the Pierce™ LDH Cytotoxicity Assay Kit (Thermo Fisher, Cat No. 88953), according to manufacturer instructions ...
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bioRxiv - Physiology 2022Quote: ... The sample was subsequently labeled with the TAMRA-alkyne dye by using the Click-It™ TAMRA Glycoprotein Detection Kit (Thermo Fisher Scientific, Waltham, MA). The mixture was vortexed for 5 seconds after the addition of each component according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... 10μM EdU was added to the culture media for 2h prior to fixation and detection using the Click-iT™ EdU Alexa Fluor™ 647 Flow Cytometry Assay Kit (Thermo Fisher Scientific, C10424), according to manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... The assessment of apoptosis in pMBMECs was performed by immunofluorescence staining with the Image-iT™ LIVE Red Poly Caspases Detection Kit (Thermo Fisher Scientific, Massachusetts, USA) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... real-time RT-PCR for AIV diagnosis was done using the VetMAX-Gold AIV Detection Kit (cutoff Ct value = 38 / internal control 25-30 Ct, Applied Biosystems™ Cat No. 4485261) and the Detection Kit Genome amplification (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... the purified PCR products were normalized to equimolar concentration with the SequalPrep Normalization Plate Kit (Thermo Fisher Scientific) and the DNA library was sequenced on the Illumina MiSeq next generation sequencing system (Illumina Inc. ...
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Hypoxia-mediated suppression of pyruvate carboxylase drives tumor microenvironment immunosuppressionbioRxiv - Cancer Biology 2022Quote: ... Labeled cDNA was hybridized to the plate using GeneTitan Hybridization Wash and Stain Kit for WT Arrays (Affymetrix).
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bioRxiv - Molecular Biology 2022Quote: ... The same plate was used for evaluating SOD (Superoxide Dismutase Colorimetric Activity Kit – Thermo Fisher Scientific – ref EIASODC) and CAT (Catalase Colorimetric Activity Kit –Thermo Fisher Scientific – ref EIACATC ...
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bioRxiv - Microbiology 2022Quote: ... The PCR products were normalized with the SequalPrep Normalization Plate Kit (Invitrogen, Thermo Fisher Scientific, Waltham, MA, USA) and eluted in 20 μL elution buffer ...
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bioRxiv - Immunology 2023Quote: ... CpG content was measured in a 96 well plate using Ribogreen™ fluorescence staining kit from Thermo Fisher and a SpectraMax i3 plate reader (Molecular devices ...
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bioRxiv - Microbiology 2023Quote: ... Duplicate PCRs were combined prior to clean up and normalization with a SequalPrep Normalization Plate kit (ThermoFisher Scientific) following manufacturer instructions using 25 µL of PCR product per sample ...
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bioRxiv - Immunology 2023Quote: ... CpG content was measured in a 96 well plate using Ribogreen™ fluorescence staining kit from Thermo Fisher and a SpectraMax i3 plate reader (Molecular devices ...
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bioRxiv - Microbiology 2023Quote: ... triplicate first-step PCR reactions were pooled and purified and normalised with the SequalPrep Normalisation Plate Kit (Invitrogen) following the manufacturers’ instructions ...
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bioRxiv - Physiology 2023Quote: ... Protein concentration was quantified on a plate reader using the Pierce BCA protein assay kit (#23225, Thermo Scientific). Gel lanes were loaded with 30 μg of total protein ...
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bioRxiv - Genetics 2021Quote: ... were used (final dilutions of 1:10000-25000) for detection by the enhanced chemiluminescence “Femto West” western blotting detection system (Thermo Fisher Scientific Inc., Rockford, IL, USA) and visualized using a ChemiDoc MP imaging system (BioRad Inc. ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Individual specimens were either flash-frozen in liquid nitrogen and stored at −80°C or fixed in RNAlater (Thermo Fisher Scientific, Waltham, MA, USA) and stored at 4°C or −20°C ...
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bioRxiv - Bioengineering 2020Quote: ... freeze-dried mycelium was ground to a fine powder using mortar and pestle under liquid nitrogen and RNA was extracted with TRIzol™ Reagent (Invitrogen; Art. No.: 15596026) according to the manual ...
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bioRxiv - Plant Biology 2021Quote: Total RNA was extracted by freezing collected samples with liquid nitrogen and grinding with a mortar and pestle with Trizol reagent (Life Technologies, Carlsbad, CA, USA). Then ...
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bioRxiv - Microbiology 2020Quote: ... Total nitrogen and carbon content of air-dried soil samples were analyzed with a Flash EA 1112 Elemental Analyzer (Thermo Fisher Scientific, Waltham, MA). For measurements of NH4+ ...
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bioRxiv - Biophysics 2020Quote: ... at 4°C and 100% humidity and plunge frozen in liquid ethane cooled to the temperature of liquid nitrogen (Thermo Fisher Vitrobot Mark IV). Cryo-EM images were collected using a 300 kV Thermo Fisher Krios microscope equipped with a Gatan K3 direct detector camera in super resolution mode ...
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bioRxiv - Biochemistry 2022Quote: ... The sample was blotted for 25 s with a force of 6 and then flash-frozen in liquid ethane and stored in liquid nitrogen using a Vitrobot Mark IV (Thermo Fisher Scientific, Waltham, USA) with the environmental chamber set at 100% humidity ...
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bioRxiv - Physiology 2020Quote: Bone biopsies stored at -80°C between 1 and 7 days were placed into liquid nitrogen contained in a ceramic mortar sprayed with RNaseZap® (ThermoFisher Scientfic, MA, USA). Samples were pulverised using an RNaseZap-treated pestle ...
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bioRxiv - Microbiology 2021Quote: ... brasiliensis cells were also lysed with the aid of a small mortar and pestle in liquid nitrogen before total RNA was isolated using Trizol reagent (Life Technologies, Carlsbad, CA, USA), as described previously [35] ...
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bioRxiv - Plant Biology 2022Quote: ... were collected 2 days post-infiltration and ground to a homogeneous powder in liquid nitrogen and resuspended in 1 mL of protein extraction buffer (1× PBS, 1% n-dodecyl- β-d-maltoside or DDM (Invitrogen, Carlsbad, CA, USA), and 0.1 tablet cOmplete™ protease inhibitor cocktail (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2023Quote: Silkworm samples were transferred into a tin capsule for carbon and nitrogen isotope ratio analysis using a stable isotope ratio mass spectrometer (IRMS, MAT-235, Thermo Fisher Scientific Inc, USA) equipped with an elemental analyzer (Flash 2000 HT ...
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bioRxiv - Plant Biology 2023Quote: ... The infected leaves were ground into powder using liquid nitrogen and total RNA was extracted using TRIzol Reagent (Thermo Fisher Scientific, Cat. No. 15596018) following the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2023Quote: ... were collected 2 days post-infiltration and ground to a homogeneous powder in liquid nitrogen and resuspended in 1 mL of protein extraction buffer (1× PBS, 1% n-dodecyl β-d-maltoside or DDM (Invitrogen, Carlsbad, CA, USA), and 0.1 tablet cOmplete™ protease inhibitor cocktail (Sigma-Aldrich ...
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bioRxiv - Immunology 2024Quote: ... The collected tissues were snap-frozen in liquid nitrogen for immunoblotting or preserved in RNAlater Stabilization Solution (Cat# AM7021; Invitrogen by Thermo-Fisher Scientific, Carlsbad, CA, USA), according to the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2019Quote: ... cells were stained with 10 μL ReadyProbes Cell Viability Imaging Kit Blue/Red (Invitrogen) per well for 30 minutes at room temperature and z-stack images of stained cells were taken using an EVOS FL Auto 2 Cell Imaging System (Invitrogen) ...
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bioRxiv - Molecular Biology 2023Quote: ... PDF electroporations were performed using the Neon Transfection System 10 µl kit (ThermoFisher #MPK1096) as previously described12 ...
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bioRxiv - Molecular Biology 2023Quote: ... eluted in 10 µL volume and quantified using Qubit dsDNA High Sensitivity Kit (Invitrogen) and was used for tagmentation using Nextera XT DNA Library Preparation Kit (Illumina).
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bioRxiv - Cancer Biology 2023Quote: ... and stained with 10 μM CellTraceTM Violet Cell Proliferation Kit (VPD) (Thermo Fisher Scientific) for 10 min at 37°C ...
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bioRxiv - Developmental Biology 2024Quote: ... 10 µg of RNA was treated with 1 µl of DNAse (Invitrogen TURBO kit) at 37 °C for 30 min ...
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bioRxiv - Biophysics 2021Quote: ... Kinetics of tubulin were measured by turbidimetry by following absorbance at 340 nm every 10 s for a period of 30 min using a plate reader (Varioskan, Thermo Scientific, USA) with samples placed in well UV-transparent half-area flat-bottomed plates (Sigma Aldrich ...
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bioRxiv - Biophysics 2020Quote: ... A single colony from the LB-agar plate with ampicillin was inoculated into 10 mL of media containing 20 g/L tryptone (Fisher Scientific, BP1421), 10 g/L yeast extract (Fisher Scientific ...
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bioRxiv - Microbiology 2019Quote: ... in the presence of 10 μg of purified Gn or NP antigens in 96 well V-bottomed culture plates (Nunc, Roskilde, Denmark). For intracellular detection of IFN-γ ...
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bioRxiv - Cell Biology 2022Quote: Day 10 of 5 plates of worms were washed with M9 buffer and incubated with 100 mM Amplex Red probe (Invitrogen, CA, USA) in Krebs-Ringer Phosphate buffer (145 mM NaCl ...
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bioRxiv - Cell Biology 2022Quote: ... 96-well plate) stably expressing GLuc-ASARTDL or GLuc-Untagged were reverse transfected with 10 nM siRNA using Lipofectamine RNAiMax (Thermo Fisher Scientific). A full media exchange was performed 48 h after transfection and after a further 24 h incubation cells were placed in hyperthermic or normothermic conditions for 24 h ...
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bioRxiv - Immunology 2022Quote: ... lentiviral vectors were produced by transfecting 5×106 HEK293T cells on T150 plate in 10 ml Opti-mem medium (Gibco, #31985-070) (containing 2% HI FBS and 100 U/ml penicillin ...
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bioRxiv - Immunology 2022Quote: BMDMs were seeded into 6-well plates (one million cells per well) in DMEM containing 10% dialyzed FBS (Thermo Scientific, Waltham, MA), 4 mM unlabeled glutamine ...
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bioRxiv - Genomics 2019Quote: ... with 2.5 μl of the diluted product in a reaction volume of 10 μl in a 384-well plate on an ABI PRISM 7900HT (Thermo Fisher Scientific) instrument with TaqMan Gene Expression Assays (Hs01902432_s1 for RAC1 and Hs99999908_m1 for GUSB ...
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bioRxiv - Cancer Biology 2019Quote: ... Reactions were diluted 4-fold with H2O and qPCR was performed in 10 μL reaction volume in 96-well plates using PowerUp SYBR Green PCR Master mix (Thermo Fisher Scientific), 2 μL diluted cDNA preparation ...
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bioRxiv - Bioengineering 2020Quote: ... screen plates were washed 3x with FluoroBrite basal media (2 mM GlutaMAX and 10 mM HEPES in FluoroBrite DMEM (Thermo Fisher Scientific)) using a BioTek 406 plate washer with 10 min incubations followed by a 1 min centrifugation at 200 rcf to settle media between washes ...
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bioRxiv - Microbiology 2019Quote: ... supplemented with 15% heat-inactivated fetal bovine serum in 96-well plates for 10–14 days in the presence of hypoxanthine–aminopterin–thymidine (HAT, Thermo Fisher Scientific). The first screening of the culture medium for antibodies specific to BoNT/A1 or BoNT/B1 was performed by ELISA ...
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bioRxiv - Cell Biology 2021Quote: The reactions to determine gene expression were conducted in three replicates with a final reaction volume of 10 μL in MicroAmp Fast Optical 96 Well Reaction Plates (Thermo Fisher Scientific) containing 1X GoTaq qPCR Master Mix (Promega Corporation) ...
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bioRxiv - Developmental Biology 2021Quote: ... 1 μL 10 mM of both forward and reverse primer and 15 ng gDNA was mixed in a 10 μL reaction volume and loaded in a white qPCR plate with optical caps (Thermo Fisher Scientific). Samples were spun briefly and run on a Mx3005P qPCR machine (Agilent ...
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bioRxiv - Developmental Biology 2021Quote: ... and 10 μL cDNA was mixed in a 10 μL reaction volume and loaded in a white qPCR plate with optical caps (Thermo Fisher Scientific). Samples were spun down briefly and run on a Mx3005P qPCR machine (Agilent ...