Labshake search
Citations for Thermo Fisher :
1901 - 1950 of 10000+ citations for Urea Nitrogen BUN Detection Kit 10 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2020Quote: ... containing 10 μg/ml DNase I (StemCell; #7469) and re-plated into 12-well plates coated with 10 μg/ml human bulk fibronectin (ThermoFisher Scientific; #3560) at a density of 750,000 cells/well ...
-
bioRxiv - Neuroscience 2021Quote: ... qRT-PCR reactions were conducted in three replicates with a final reaction volume of 10 µL in MicroAmp Fast Optical 96 Well Reaction Plates (Thermo Fisher Scientific) containing 1X GoTaq qPCR Master Mix (Promega Corporation) ...
-
bioRxiv - Cell Biology 2021Quote: ... were cultured in a 6-well plate in 1mL of media (DMEM, Thermofisher Cat# 12430054; 10% FBS, FisherSci Cat# 10437028; 1x Anti-Anti, Thermofisher Cat# 15240062) with 8ug of polybrene and 100uL of 10x pLVX-cOVA-Puro (Addgene Cat#135073 ...
-
bioRxiv - Cell Biology 2022Quote: ... cells grown to 80% confluency in 6-well or 10 cm plates were washed once in cell culture-grade PBS (Gibco, 10010-015), scrapped in ice-cold cell culture-grade PBS and collected at 300 g and 4°Cfor 5 min in a 1.5 ml microfuge tube ...
-
bioRxiv - Cell Biology 2019Quote: ... Collagen/laminin coating was accomplished by overnight incubation of the plates rocking at 4°C with a 1:1 mixture of laminin (10 µg/ml ThermoFisher 23017-015) and collagen (10 µg/ml Sigma C8919 ...
-
bioRxiv - Microbiology 2020Quote: ... the plates were revealed using a citrate solution containing o-phenylenediamine dihydrochloride (OPD, Thermo Scientific 34006, 10 min incubation in the dark). The reaction was stopped using 2N H2SO4 and the plate read at OD 450 nm.
-
bioRxiv - Molecular Biology 2019Quote: ... and HepG2 (1E5 cells/well) cells were seeded in 24-well plates and cultured in 1x GlutaMAX™-I DMEM supplemented with 10% FBS (Thermo Fisher). The next day the cells were transfected with 50 nM tRF-mimics or tRF antisense 2’ OMe-RNAs (biomers ...
-
Significant control of Zika infection in macaques depends on the elapsing time after dengue exposurebioRxiv - Immunology 2019Quote: ... 8.5 × 104 cells/well in 24 well plates with growth medium (Dulbecco’s Modified Eagle’s medium, Thermo Fisher Scientific, with 10% FBS (Gibco), 1% non-essential amino acids (Gibco) ...
-
bioRxiv - Immunology 2021Quote: ... Samples were then plated in 96-well flat bottom plates at a ratio of 25:75 with CD45.1 splenocytes and stimulated in 10% HI-FBS RPMI-1640 (ThermoFisher Scientific cat. 11875085) containing Brefeldin A (eBioscience cat ...
-
bioRxiv - Immunology 2020Quote: ... DOPC and DOPS liposomes prepared in TBS were immobilized at 10 µg/ml in binding buffer (TBS, 0.1% BSA) on streptavidin-coated high capacity plates (Thermo Fisher, Waltham, MA). Increasing concentrations (0.01-10 µg/ml ...
-
bioRxiv - Immunology 2022Quote: Recombinant human β2m-MR1-K43A-Fc was produced in CHO-K1 cells as previously described (10) and 4 µg/mL were coated onto 96 wells plates (Nunc, Cat#439454) for 18 h at 4°C ...
-
bioRxiv - Biochemistry 2022Quote: ... cells were trypsinized and seeded in a 10-cm plate using growth media containing 100 U/mL penicillin and 100 μg/mL streptomycin (Gibco, ThermoFisher Scientific), 4 μg/mL blasticidin (Gibco ...
-
bioRxiv - Biochemistry 2022Quote: ... Reaction solutions in the cell-free protein synthesis system were each diluted 10-fold with water following the reaction and 40 µL per well dispensed into Flat Bottom Microfluor Plates Black (Thermo Fisher Scientific), and the fluorescence intensity was measured (excitation 485 nm (bandwidth 9 nm)/emission 535 nm (bandwidth 20 nm) ...
-
bioRxiv - Microbiology 2022Quote: ... Three 10-μl drops of the matrix-cell suspension were pipetted into wells of a Nunclon Delta 24-well plate (Thermo Fisher Scientific) and were solidified for 15 minutes at 37°C before adding 400 μl per well of culture medium (Supplementary Table 1) ...
-
bioRxiv - Microbiology 2022Quote: ... faecalis V583 overnight cultures were diluted 10-fold into 200 μL complete DMEM in black-walled 96-well plates containing 50 μM DHR123 (Thermo Fisher Scientific), vehicle (DMSO) ...
-
bioRxiv - Synthetic Biology 2023Quote: BT-549 and hTERT-HME1 cells were grown to ~80% confluency in 10 cm tissue culture-treated plates and harvested with 0.25% trypsin-EDTA (Thermo Fisher Scientific, 25200056). Lysates were extracted using RIPA lysis buffer and insoluble protein was cleared by centrifugation at 16,000xg for 30 min ...
-
bioRxiv - Microbiology 2022Quote: ... washed twice with 20 mM MOPS buffer (pH 7) and concentrated to reach a final A578 of 10 in a 96 well-plate (ref. 4483481, Applied Biosystems™) containing cytochrome c (0.25 mg/ml ...
-
bioRxiv - Microbiology 2023Quote: ... 5×104 HEK 293T and 2.5×104 MDCK-SIAT1 cells were plated in 12 well plates in growth media (DMEM, Invitrogen #19965-092, with a final concentration of 10% Fetal Bovine Serum (FBS, Invitrogen #26140-079), 100 units/mL Penicillin (Invitrogen #15140-122) ...
-
bioRxiv - Molecular Biology 2023Quote: ... After 24 hr post-transfection cells were cultured at low density (5000 cells/10 cm plate) and treated with 200 μg/ml G418 (Thermo Fisher, UK) in ESC cell media for 10 days ...
-
bioRxiv - Microbiology 2023Quote: ... 10 x 1 µL droplets of each diluted suspension were deposited on polystyrene material (6-well plates, Thermo Scientific, Cat. No. 140675) and exposed to ambient temperature and variable RH (20% ...
-
bioRxiv - Immunology 2023Quote: ... coated plates were washed three times with PBS and blocked with RPMI-1640 medium containing 10% FBS (Life Technologies, Cat.#A0336-50ML) at 37 °C for one and a half hours ...
-
bioRxiv - Synthetic Biology 2022Quote: ... aureus.The DNA sensor strains were incubated at 37°C with shaking (250 rpm) for 10 hours and 5 μL of cell culture was plated onto 12-well plates (Thermo Fisher Scientific) containing 1 mL LB agar supplemented with 2 mM IPTG ...
-
bioRxiv - Synthetic Biology 2022Quote: ... The DNA sensor strains were incubated at 37°C with shaking (250 rpm) for 10 hours and 5 μL of cell culture was plated onto 12-well plates (Thermo Fisher Scientific). In these plates ...
-
bioRxiv - Cell Biology 2023Quote: ... coated 6-well plates at 5×105 cells/well in RPMI 1640 with B27 and 10% knockout serum replacement (Thermo Fisher Scientific). Cells were expanded by culturing in RPMI 1640 with B27 with 2 μM of CHIR99021 (Stemcell Technologies) ...
-
bioRxiv - Molecular Biology 2023Quote: ... cells were plated at 4×105 cells in 60mm tissue culture plates in “growth medium”: DMEM (high glucose, without pyruvate; Thermo-Fisher cat # 11965092) with 10% fetal bovine serum (Gibco cat # A3160501); no antibiotics were used ...
-
bioRxiv - Molecular Biology 2023Quote: ... 50 µL of subculture was added to 50 µL of 1X phosphate buffered saline (PBS) with 10 µg/mL of kanamycin in a 96-well optical bottom black plate (Thermo Scientific, #265301). Bulk optical density (OD600 ...
-
bioRxiv - Physiology 2023Quote: ... The real-time qPCR amplification was performed in 10 μl reaction mix in a 384-well plate using a QuantStudio™ 5 instrument (ThermoFisher Scientific). All runs started with an initial denaturation step of 2 min at 95 °C ...
-
bioRxiv - Physiology 2023Quote: ... The formazan crystal pellets were dissolved in DMSO and the plate was read 10 min later using Multiskan FC Microplate Photometer (Thermo Fisher, USA) at 550 nm.
-
bioRxiv - Cancer Biology 2023Quote: ... 5 × 105 cells were seeded in 6-well plates and immediately transfected with a mixture of 10 μL Lipofectamine2000 (Thermo Fisher Scientific), 6 μL of 20 μM target-specific siRNA (or non-targeting siRNA as control) ...
-
bioRxiv - Cancer Biology 2024Quote: ... The plate was then replenished with 15 mL of pre-warmed 293T culture medium (DMEM, 10% fetal bovine serum (Fisher Scientific, SH3007103) and 1X Penicillin-Streptomycin ...
-
bioRxiv - Neuroscience 2024Quote: ... the cells were centrifuged at 200× g for 10 min and cultured in 24 well-plates in StemPro-34 complete medium (Thermo Fisher Scientific) supplemented with 200 mM GlutaMAX (Thermo Fisher Scientific) ...
-
bioRxiv - Biochemistry 2024Quote: ... The solutions were mixed for 10 s at 600 rpm in a Varioscan LUX plate reader (Thermo Fisher Scientific, Waltham, MA, USA). Dopachrome generation was detected by recording the optical absorbance at 490 nm for a period of 72 min.
-
bioRxiv - Biophysics 2021Quote: ... The filtered lysate was diluted 1:2 in purification buffer UA (8 M Urea, 50 mM sodium acetate [Acros Organics, Carlsbad, CA], pH 4). The protein was then purified using a cation exchange HiPrep SP HP 16/10 (Cytiva ...
-
bioRxiv - Molecular Biology 2020Quote: ... 4μg of total RNA were loaded per well after denaturation by heating at 70°C for 3 minutes in sample buffer (Novex TBE-Urea sample buffer; Thermo fisher Scientific, Catalog# LC6876). Samples were electrophoresed using Novex 15% TBE-Urea Gels (Invitrogen ...
-
bioRxiv - Biochemistry 2023Quote: ... The samples were separated on a 15% denaturing urea-PAGE and visualized via SYBR™ Gold Nucleic Acid Gel Stain (Invitrogen by Thermo Fisher).
-
bioRxiv - Cancer Biology 2023Quote: ... prior to lysis with the proteomics lysis buffer (8M Urea, 50mM triethylammonium bicarbonate) supplemented with Halt Protease and Phosphatase Inhibitor Cocktail (Thermo Fisher Scientific, Cat. 78440). The lysates were sonicated for 30 seconds on ice (amplitude 25% ...
-
bioRxiv - Microbiology 2020Quote: ... Tissue samples harvested for vRNA detection were immersed in RNAlater (Ambion) 4 °C for 1 day ...
-
bioRxiv - Cancer Biology 2021Quote: ... Signals were detected using enhanced chemiluminescence detection reagents (Thermo Fisher Scientific) and images were acquired using a luminescent image analyzer (LAS3000 ...
-
bioRxiv - Genetics 2021Quote: ... Detection was performed using PierceTM ECL western blotting Substrate (ThermoFisher scientific).
-
bioRxiv - Developmental Biology 2021Quote: ... Alexa Fluor 680 plus were used for immunofluorescence detection (Molecular Probes and Jackson ImmunoResearch Laboratories ...
-
bioRxiv - Developmental Biology 2020Quote: ... Data analysis was done using Viia7 sequence detection interface (Applied Biosystems). mRNA expression for various targets was determined relative to mouse or human GAPDH levels using ΔΔCt method.
-
bioRxiv - Developmental Biology 2020Quote: ... Antibody detection was developed with West Femto Substrate (Thermo Scientific 34095). Densitometry was preformed using Fiji software “gel analysis” plug-in.
-
bioRxiv - Neuroscience 2021Quote: ... on an ABI PRISM 7700 sequence detection system (Applied Biosystems Instruments). Normalization was performed using 18S rRNA as reference gene ...
-
bioRxiv - Immunology 2021Quote: ... and the Applied Biosystems 7900HT Sequence Detection (ThermoFisher, Waltham, MA, USA) and sequenced on a Illumina NovaSeq S2 flowcell (Illumina ...
-
bioRxiv - Immunology 2021Quote: ... and the Applied Biosystems 7900HT Sequence Detection (ThermoFisher, Waltham, MA, USA) and sequenced on an Illumina NovaSeq system (Illumina ...
-
bioRxiv - Plant Biology 2019Quote: ... HRP activity was detected by Supersignal Western Detection Reagents (Thermo Scientific), and was then visualized with an Amersham 600RGB molecular imaging system (GE Healthcare).
-
bioRxiv - Immunology 2019Quote: ... the CellEvent Caspase-3/7 Green Detection Reagent (Thermo Fisher Scientific) was added to the cells with complete media for 30 min in 37°C and the caspase-3/7 positive cells were measured on an Attune NXT flow cytometer (Life Technologies ...
-
bioRxiv - Molecular Biology 2019Quote: ... Western blotting detection was performed using anti-GFP (ThermoFisher Scientific, A11122) or anti-HA antibody (Abcam ...
-
bioRxiv - Plant Biology 2019Quote: ... Detection was performed with the Pico Plus system (34580, Thermo Scientific) and X-ray films (47410 19284 ...
-
bioRxiv - Cancer Biology 2019Quote: ... on a 7900 HT sequence detection system (Applied Biosystems, ThermoFisher Scientific). Cycling conditions were 95°C ...