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Citations for Thermo Fisher :
1601 - 1650 of 10000+ citations for Urea Nitrogen BUN Detection Kit 10 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... PANX2 or PANX3 plasmids in a 10 cm plate (Penuela et al., 2007; Penuela et al., 2009) using Lipofectamine 3000 (Invitrogen) according to the manufacturer’s protocol.
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bioRxiv - Pathology 2022Quote: ... One million myoblasts per genotype and per mouse were pelleted at 300g for 5 minutes in 15 mL centrifuge tubes before pellets were transferred into individual wells of a 6-well plate containing 50 % proliferation medium (20 % foetal bovine serum, Gibco 10500-064; 10 % horse serum, Gibco 26050-088 ...
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bioRxiv - Biochemistry 2022Quote: ... Day 1 – 0.5 x 105 cells were seeded on fibronectin-coated coverslips in a 24-well plate for immunofluorescence or 3 x 105 cells in a 6-well plate for western blotting and transfected with 20 nM siCAPN7 and 10 nM siNup153 complexed with Lipofectamine RNAiMAX (Invitrogen) (See Supplementary Table 3 for sequences ...
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bioRxiv - Immunology 2022Quote: ... HEK293T/17 cells were maintained in 10 cm plates and passaged after reaching 90% confluency with 2 mL of Trypsin-EDTA (0.25%) (Gibco, 25200056) at 37°C for 2 minutes until passage 50 ...
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bioRxiv - Cell Biology 2022Quote: HeLa Flp-In T-REx cells with an insertion of dox-inducible FLAG-SLC3A2 were seeded (2 x 106 cells per 10 cm plate (Nunc) in DMEM high glucose media plus 10% FBS ...
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bioRxiv - Microbiology 2024Quote: ... OD600 was adjusted to 1 and 2.5 μl were mixed with 500 μl of RPMI + 1% CA + 10 μM EDDHA per well (final OD600 of 0.005) in a 48 well microtiter plate (Nunc, Thermo Scientific). 200 ng/ml aerobactin (EMC Microcollections GmbH) ...
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bioRxiv - Molecular Biology 2024Quote: ... or 24 well plates (5×104 cells/well) with or without coverslip and induced with tetracycline (10 μg/ml, Invitrogen) for 48 hours ...
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bioRxiv - Cell Biology 2024Quote: ... 10 μg lentiviral vectors with 6 μg psPAX2 and 4 μg pMD2.G were packaged into an 80% confluent 10 cm plate of HEK293T cells (ATCC) with 6ml Opti-MEM by using Lipofectamine 2000 (Invitrogen) with another 1.5 ml Opti-MEM (Invitrogen) ...
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bioRxiv - Immunology 2024Quote: ... primary Rhinolophus ferrumequinum bat cells were seeded in 6-well plate at a density of 6×105 cells/well with DMEM containing 10% FBS (Gibco). After 12 h ...
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bioRxiv - Immunology 2024Quote: ... Tissue was added to 96-well round bottom plates in 100μL of R10 (RPMI supplemented with 10% FBS, 1% penicillin/streptomycin, 1X Glutamax (Thermo Scientific), and 55μM b-mercaptoethanol) ...
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bioRxiv - Immunology 2024Quote: ... Cells were counted and 4 million cells were plated in 10 cm non-TC treated plates in 8 mL RPMI with HEPES (Gibco), Sodium Pyruvate (Gibco) ...
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bioRxiv - Cell Biology 2023Quote: ... and seeded onto 384-well plates at a volume of 10 µl per well using the Matrix WellMate (Thermo Fisher) and then spun down at 1200 rpm for 2 min ...
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bioRxiv - Cancer Biology 2023Quote: ... The cell lines were grown in 10 cm diameter plates in a humidified incubator (37°C, 5% CO2) with RPMI 1640 medium (Gibco) containing 10% of fetal bovine calf serum ...
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bioRxiv - Microbiology 2023Quote: ... OD600 was adjusted to 1 and 2.5 μl were mixed with 500 μl of RPMI + 1% CA + 10 μM EDDHA per well (final OD600 of 0.005) in a 48 well microtiter plate (Nunc, Thermo Scientific) or 0.5 μl were mixed with 100 μl RPMI + 1% CA + 10 μM EDDHA per well in a 96 well microtiter plate (Falcon flat bottom ...
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bioRxiv - Immunology 2023Quote: ... in LUV buffer (10 mM HEPES pH 7.5, 135 mM KCl) and aliquoted into a 96 well flat-bottom plate (ThermoFisher Scientific). Liposomes were purified from excess dye using a PD-10 desalting column (Cytiva ...
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bioRxiv - Cell Biology 2023Quote: ... and seeded onto 0.1% gelatine-coated 6-well plates in DMEM supplemented with 10% foetal calf serum (Gibco, ThermoFischer, #26140079) and 1x Glutamax (Gibco ...
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bioRxiv - Cell Biology 2023Quote: ... The non-attached phase was recollected and replated in plates coated with 10 μg/ml laminin (Life Technologies cat# 23017015) in DMEM supplemented with 10% fetal bovine serum and antibiotic/antimycotic solution ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were seeded onto 6-well or 96-well plates for 24 hours and followed by siRNA (10 nM) transfection using Lipofectamine RNAiMAX (Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: ... 293T cells were transfected with plasmids encoding lentiviral structural components (pMDL, pREV, VSVG) and payloads (PPARG2 or rtTA, at 10 μg per planned MPC plate) using Lipofectamine 3000 (Invitrogen) in low-serum ...
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bioRxiv - Cancer Biology 2023Quote: ... 1×104 cells per well in a 96-well plate were treated with 50% serum free CAF condition medium supplemented with 10% FBS (cat. no. 10270-106; Gibco) and 50% fresh RPMI medium for 72 h ...
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bioRxiv - Developmental Biology 2023Quote: siRNA knockdown was performed as previously reported.20 9-12 week placental explants were placed into a 48-well plate with IMDM supplemented with 10% (v/v) FCS and gentamicin (50 μg/mL; Gibco). siRNA sequences targeting PRKCI (final concentration 0.2 μM ...
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bioRxiv - Cancer Biology 2023Quote: Cells were cultured to approximately 60% confluency on 24-or 6-well plate and transfected with siRNAs at final concentrations of 10-25nM using lipofectamine RNAiMAX (Invitrogen) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... naturally expressing TDO were cultured in tissue culture treated plates containing EMEM (ATCC # 30-2003) medium containing 10% FBS (Gibco) until 90% confluent after which cycloheximide (Sigma # C7698 ...
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bioRxiv - Neuroscience 2023Quote: ... with fewer than 15 passages onto 12-well plates in HEK-293 growth medium (DMEM [Gibco # 10566-061] with 10% FBS [Gibco #16140-071] and 1x Pen Strep [Gibco #15070-63] ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were grown on tissue culture (treated) plates in DMEM/F12 media without phenol red supplemented with 10% charcoal-stripped FBS (Gibco), 2mM L-Glutamine ...
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bioRxiv - Cancer Biology 2023Quote: ... and primary OC-CAFs was assessed by plating 1.0 × 10^5 cells/mL in triplicates in 96-well flat bottom plates (Fisher Scientific) and growing for 7 days between cell passages before trypsinization ...
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bioRxiv - Bioengineering 2024Quote: ... NPCs were expanded into 10 cm plates in Basal Neural Maintenance Media (BNMM) (1:1 DMEM/F12 (#11039047 Life Technologies) + glutamine media/neurobasal media (#21103049 ...
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bioRxiv - Bioengineering 2024Quote: ... NPCs were expanded into 10 cm plates in Basal Neural Maintenance Media (BNMM) (1:1 DMEM/F12 (#11039047 Life Technologies) + glutamine media/neurobasal media (#21103049 ...
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bioRxiv - Cell Biology 2024Quote: ... Freshly harvested primary monocytes were plated at a density of 20-25 M per 10 cm plate in macrophage culture media containing: RPMI (11875119, ThermoFisher), 10% FBS (26140079 ...
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bioRxiv - Developmental Biology 2024Quote: ... L3-L4 well-fed animals were washed from a plate with M9 buffer and incubated for three hours in 10 μg/ml DiI solution (D282, ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... Biotinylated TACC3 Δ699-765 (50 µL of 10 µg mL−1, diluted in PBS) was immobilized on preblocked HBC Streptavidin plates (ThermoFisher) for 30 min at room temperature (RT) ...
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bioRxiv - Neuroscience 2020Quote: Lysates of worms were prepared by sonication in buffer consisting of 4% urea and 0.5% SDS with 1 x Halt™ Protease and Phosphatase inhibitor Cocktail (Thermo Fisher Scientific). HEK293 cell lysates were prepared using RIPA buffer consisting of 150 mM NaCl ...
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bioRxiv - Cell Biology 2021Quote: ... An aliquot of lysate was taken and diluted 1:5 in 8 M Urea from which protein concentration was determined by BCA assay (Thermo Fisher Scientific). Aliquots corresponding to 100 μg of protein were subsequently diluted in SDC buffer into a 96-well deep-well plate ...
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bioRxiv - Cancer Biology 2022Quote: ... Samples were resolved by electrophoresis at 150 V for 3 h at room temperature in Novex TBE-Urea Gels (EC6885BOX, Thermo Fisher Scientific) in 1x TBE buffer and cleaved and uncleaved products were imaged on ChemiDoc (Bio-Rad ...
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bioRxiv - Cell Biology 2021Quote: ... alkylated using iodoacetamide in 8M urea denaturation buffer (50mM Tris-HCl, pH 8) and digested overnight in 50mM ammonium bicarbonate using trypsin (Thermo Scientific #90057) at 37°C ...
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bioRxiv - Molecular Biology 2021Quote: ... followed by separation on 20% 7 M urea SDS-PAGE gel and immunoblot assay with anti-Biotin antibody (Thermo Fisher Scientific, 692033).
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bioRxiv - Microbiology 2022Quote: ... The RNA samples were then separated by 12% Urea-PAGE followed by gel extraction and subsequent reverse transcription using Superscript III reverse transcriptase (Invitrogen #18080–044). The cDNA was PCR amplified and sequenced on Illumina Hi-Seq 4000 ...
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bioRxiv - Biochemistry 2024Quote: ... an aliquot of the reaction mixture was pipetted out at the indicated time points and quenched by mixing with an equal volume of 2X TBE/Urea sample buffer (Thermo Fisher Scientific) and heating at 65 °C for 20 min ...
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bioRxiv - Molecular Biology 2024Quote: ... The purity of all newly synthesized RNAs was detected using UREA-polyacrylamide gel electrophoresis (PAGE) which was stained with SYBR™ Gold (Invitrogen, S11494). The gels were finally visualized on a ChemiDoc MP imaging system (Bio-Rad).
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bioRxiv - Genomics 2023Quote: ... samples (diluted in 2x Novex TBE-Urea sample buffer) and DNA ladders (Low Range and 100 bp or 1 kb Plus, Thermo Fisher Scientific) were boiled at 95°C for 5 minutes then were run on 10-15% TBE-Urea gels for 30-60 minutes at 70°C using a circulating water bath hooked up to the gel cassette ...
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bioRxiv - Biochemistry 2023Quote: ... The samples were separated on a 15% denaturing urea-PAGE and visualized via SYBR™ Gold Nucleic Acid Gel Stain (Invitrogen by Thermo Fisher).
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bioRxiv - Genetics 2023Quote: ... 5 µg of small RNA fraction was run on 15% TBE/Urea gel using Owl™ S4S sequencing gel system (Thermo Scientific) and small RNAs of 20- 35 nt were gel-purified for library construction (input) ...
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bioRxiv - Neuroscience 2020Quote: ... ELISA plates (Nunc, MaxiSorp, 96 well plates, 44-2404-21) were coated with 100ul human C3b at 3 μg/mL in PBS (covered ...
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bioRxiv - Cell Biology 2022Quote: ... 96 well microtiter plate Maxi-Sorp micro-titter plates (Nunc International ...
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bioRxiv - Neuroscience 2020Quote: ... The plate was then applied to a plate magnet (Invitrogen) until the solution cleared and the supernatant was removed without disturbing the beads ...
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bioRxiv - Microbiology 2021Quote: ... ELISA plates (96-well plates, Immunol 4HBX, Thermo Fisher Scientific) were coated with either spike receptor binding domain (S-RBD ...
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bioRxiv - Microbiology 2020Quote: ... ELISA plates (F96 maxisorp Nunc-immuno plates, Nunc, Roskilde, Denmark) were coated with 140 μL of a suspension containing formalin-inactivated Salmonella Typhimurium varietas Copenhagen strain DAB69 bacteria diluted in coating buffer to an optical density (OD ...
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bioRxiv - Microbiology 2020Quote: ... ELISA plates (F96 maxisorp Nunc-immuno plates, Nunc, Roskilde, Denmark) were coated with 140 μL of a suspension containing formalin-inactivated Salmonella Typhimurium varietas Copenhagen strain DAB69 bacteria diluted in coating buffer to an optical density (OD ...
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bioRxiv - Immunology 2022Quote: ... flat-bottomed 96-well plates (Nunc Cell-Culture Plates, ThermoFisher) were coated with 1 µg/ml anti-CD3 and 2 µg/ml anti-CD28 antibodies (BioLegend ...
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bioRxiv - Immunology 2022Quote: ... flat-bottomed 96-well plates (Nunc Cell-Culture Plates, ThermoFisher) were coated with 1 µg/ml anti-CD3 and 2 µg/ml anti-CD28 antibodies (BioLegend ...