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Citations for Thermo Fisher :
1601 - 1650 of 10000+ citations for SARS CoV 2 Spike Glycoprotein S1 Sheep Fc Tag HEK293 Biotin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2021Quote: ... The lysate was cleared by centrifugation and incubated for 2 hours at 4°C with Tag specific magnetic beads (Dynabeads™ M-280 Streptavidin/Invitrogen for immunoprecipitation of Avitag-ZFP462 and Avitag-GLP ...
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bioRxiv - Molecular Biology 2021Quote: ... Gene fragments encoding multiple (2 to 9) HA-tags were designed in silico and ordered using GeneArt Gene Synthesis service from Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... Washed cells were incubated with PBS2+ containing 2 mg mL−1 Pierce Premium Grade Sulfo-NHS-SS-Biotin (PG82077; Thermo Scientific) for 30 min on ice ...
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bioRxiv - Genomics 2022Quote: ... resuspended in ice cold PBS+BE (1 mg/mL BSA + 2 mM EDTA) and incubated for an hour at +4°C with Dynabeads Biotin Binder (Thermo Fisher). Cells were repeatedly washed with ice-cold sterile PBS while on magnet ...
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bioRxiv - Cancer Biology 2021Quote: ... and incubated in click reaction buffer (10 mM sodium-l-ascorbate, 20 μM biotin azide [Life Technologies], and 2 mM CuSO4) for 2h at room temperature ...
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bioRxiv - Cancer Biology 2023Quote: ... and incubated in click reaction buffer (10 mM sodium-l-ascorbate, 20 µM biotin azide [Life Technologies], and 2 mM CuSO4) at RT for 2 hours on a rotator ...
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bioRxiv - Immunology 2019Quote: ... whole stomachs were finely minced in disassociation buffer (2% FCS in Hank’s Balanced Salt Solution without calcium and magnesium (Life Technologies) and 5 mM EDTA ...
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bioRxiv - Immunology 2021Quote: Released tissue cells were stained in aliquots of 1 ×106 cells/100 μL of PBS with 2% fetal calf serum (FCS, GIBCO) and 0.1% NaN3 for 30min on ice ...
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bioRxiv - Immunology 2021Quote: ... NK cells were then expanded for 6 days with hIL-15 50ng/ml (Miltenyi #130-095-765) in RPMI 20% FCS supplemented with L-glutamine (2 mM; Gibco), penicillin/streptomycin (100 μg/mL ...
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bioRxiv - Immunology 2022Quote: ... F-actin staining was performed at 4°C overnight in PBS 1x containing 0,1% TritonX100 2% FCS with Phalloidin AF405plus (Life technologies). Samples were washed in PBS 1x and mounted with Prolong Antifade Gold mounting medium (Life technologies) ...
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bioRxiv - Developmental Biology 2021Quote: ... 4° C following by re-suspending the cells in 0.5-1 of ml cell isolation media (2% FCS, DPBS:HBSS=1:1 and 1mM SYTOX Blue Dead Cell stain (ThermoFisher Scientific). Cells were imaged before FACS and after FACS to confirm successful purification of GFP+ cells.
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bioRxiv - Immunology 2020Quote: ... gradient centrifugation and resuspended at 1-2 x 106 cells/ml in RPMI-10% FCS (RPMI 1640 w/L-glutamine (Cellgro Gibco) supplemented with 10% FCS (Cellgro Gibco) ...
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bioRxiv - Immunology 2021Quote: ... mice were directly perfused with PBS/ 2 % FCS/ 1 mM EDTA and liver digest medium (ThermoFisher Scientific/ Gibco, Waltham, Massachusetts) through the vena cava ...
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bioRxiv - Microbiology 2021Quote: ... The plate was incubated for approximately 5 min before the reaction was stopped with the addition of 2 M H2SO4 and the A450 was measured (Multiskan FC, ThermoFisher). A standard curve was established using serial dilutions of lysate from PA11 ...
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bioRxiv - Immunology 2022Quote: ... 2020) were cultured in R10 medium (RPMI1640 [Gibco Life Technologies, 31870-025], 10% FCS, 2 mM glutamate [Gibco Life Technologies, 25030-024] ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Content of two ampules containing the cell culture was resuspended in Hank’s solution supplemented with 2% FCS (fetal calf serum) (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... After two washes of the tissue with plating medium (DMEM containing 10% FCS, 2 mM glutamine, 50 mg/ml penicillin/streptomycin, Invitrogen), it was triturated in plating medium and subsequently cells counted ...
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bioRxiv - Microbiology 2023Quote: ... Cells were transfected with plasmids expressing bRSV N-GFP and P in DMEM supplemented with 2% FCS.16 h post transfection medium was replaced with Leibovitz’s L-15 medium without phenol red (Gibco, ThermoFisher Scientific), and imaged live with a Leica TCS SP8 confocal microscope using a 63× oil immersion objective ...
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bioRxiv - Synthetic Biology 2023Quote: ... cells were washed with PBS and resuspended in 200 µl buffer (PBS supplemented with 2% FCS) and analyzed via Attune NxT Flow Cytometer (ThermoFisher). For LIVE/DEAD analysis all cells were gated and viable cells were quantified as indicated in Figure 4 via FlowJo (Version 10.9.0 ...
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bioRxiv - Bioengineering 2023Quote: ... cells in each well were firstly washed with flow cytometry staining buffer: 3% FCS with 2 mM EDTA (15575020; Invitrogen) in PBS− ...
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bioRxiv - Genetics 2023Quote: ... resuspended in 800 μL of PBS supplemented with 2 % FCS and analyzed on Attune NxT Flow Cytometer (Thermo Fisher Scientific).
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bioRxiv - Immunology 2024Quote: ... Enzymes were quenched with PBS with 2mM EDTA and 2% FCS on ice and then red blood cells were lysed with room-temperature ACK lysis buffer (Gibco) and passed through a 100μm cell strainer.
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bioRxiv - Immunology 2023Quote: ASCs (FVS780-CD20-CD3-CD14-CD16-CD19+CD27hiCD38hi) were sort-purified from human peripheral blood mononuclear cells into culture media (10% FCS, 2 mM L-glutamine (Gibco), 100 units/mL Penicillin and 100 ng/mL Streptomycin (Sigma-Aldrich) ...
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bioRxiv - Molecular Biology 2020Quote: ... ERCC spike in mix at 1 in 20Mio dilution (Ambion #4456740). Plates were sealed (Thermo Fisher Scientific #AB0626 ...
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bioRxiv - Genomics 2019Quote: ... ERCC RNA spike-in Mix which contains 92 transcripts (Thermo Fisher) was added and processed in parallel with poly-A RNA ...
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bioRxiv - Cell Biology 2021Quote: ... Libraries included ERCC ExFold RNA spike-in mixes (Thermo Fisher Scientific) to assess the platform dynamic range ...
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bioRxiv - Cell Biology 2021Quote: ... External RNA Controls Consortium (ERCC) spike-in control from Invitrogen (#4456740) was added to each sample to minimize external sources of variability ...
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bioRxiv - Developmental Biology 2021Quote: ... We also added the ERCC spike-in mix (Thermo Fisher, 4456740) to the Lysis Mix ...
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bioRxiv - Immunology 2021Quote: ... Biotinylated Spike and RBD were expressed in Expi293F cells (Thermofisher Scientific). Supernatants were harvested after 7 days and purified ...
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bioRxiv - Immunology 2021Quote: ... Spike protein was conjugated to Alexafluor 647 microscale labeling kit (Invitrogen). The fluorescently labelled spike protein was incubated with the pooled B cells at a concentration of 0.5 µg/ml for 30 mins on ice ...
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bioRxiv - Neuroscience 2022Quote: ... except that ERCC ExFold RNA Spike-In Mixes (ThermoFisher cat. 4465739) were added according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2022Quote: ... The full Delta-Omicron recombinant spike was cloned into pcDNA6 (Invitrogen). Point mutations were introduced by overlap extension PCR ...
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bioRxiv - Genetics 2022Quote: ... An ERCC RNA Spike-In Mix kit (ThermoFisher Scientific cat. 4456740) was used to normalize total RNA prior to library preparation ...
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bioRxiv - Cell Biology 2023Quote: ... 1:6,000,000 dilution of ERCC RNA spike-in mix (Ambion – 4456740), 1 mM dNTPs (NEB - N0446S) ...
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bioRxiv - Molecular Biology 2023Quote: ... and we added 2μl of 1x LP PFP spikes (Thermo Fisher). Detailed descriptions of the spike-in controls can be found in the exRNAQC study (Consortium et al. ...
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bioRxiv - Genomics 2023Quote: ... with the following exception: ERCC spike-ins (ThermoFisher Scientific, Waltham, MA) were added to the RNA after the extraction procedure ...
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bioRxiv - Genetics 2023Quote: ... The External RNA Controls Consortium spike-in (ERCC, Ambion, Austin, TX) was added to the samples to allow for normalization in accordance with the core facility’s standard operating procedure but was not used in our downstream analyses ...
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bioRxiv - Microbiology 2023Quote: ... codon-optimized spike expression plasmid using Lipofectamine 2000 (Invitrogen™ 11668019) following manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... anti-S-tag (Thermo Scientific, #MA1-981), anti-TMEM43 (Abcam ...
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bioRxiv - Microbiology 2019Quote: ... we used Dynabeads™ His-Tag (Invitrogen) for Ni-affinity purification ...
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bioRxiv - Bioengineering 2022Quote: 6x-His tag antibodies (Invitrogen, MA1-21315) were coated at 2 µg/mL in PBS onto 96-well half- area high binding plates (Corning ...
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bioRxiv - Biochemistry 2020Quote: ... mouse anti-V5-Tag (46-0705, Invitrogen), mouse anti-Myc-Tag (9B11 ...
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bioRxiv - Neuroscience 2020Quote: ... tag from pcDNA3.1-nV5-DEST vector (Invitrogen). To generate AP2M1 or LRRK2 single or multiple mutants ...
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bioRxiv - Microbiology 2022Quote: ... anti-FLAG tag (Thermo Fisher (PA1-984B)).
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bioRxiv - Microbiology 2021Quote: ... or DYKDDDDK Tag Monoclonal Antibody (FG4R) (Invitrogen) (1 ...
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bioRxiv - Biochemistry 2021Quote: ... and V5-tag (R96025) from Life Technologies.
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bioRxiv - Cell Biology 2019Quote: ... mouse Flag-Tag monclonal antibody(Thermo Fisher, WB 1:1000 dilution ...
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bioRxiv - Microbiology 2020Quote: ... anti-KDDDDK Tag antibody (Invitrogen, MA1-91878), Monoclonal antibody anti-GAPDH produced in mouse (SIGMA ...
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bioRxiv - Cell Biology 2020Quote: ... 6x-His Tag antibody is from ThermoFisher Scientific (MA1-21315-D550) ...
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bioRxiv - Biochemistry 2021Quote: ... mouse anti-His Tag (Invitrogen MA1-21315). Secondary antibody used are ...