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Citations for Thermo Fisher :
1801 - 1850 of 1930 citations for N Nitroso Di N Propylamine D14 98% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2022Quote: ... supernatant discarded and the cell pellet dissolved by suspension in 300 µL of N-PER™ Neuronal Protein Extraction Reagent (Thermo Scientific catalog number: 87792) lysis buffer ...
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bioRxiv - Microbiology 2022Quote: ... After staining of the transferred proteins with amido black the cleavage product was cut out for sequencing via N-terminal Edman degradation performed on a Procise Protein Sequencer 494C (Applied Biosystems, Foster City, United States).
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bioRxiv - Neuroscience 2022Quote: Total RNA was extracted from hiPSC-derived cortical neurons at DIV8 (Q5, Q6, n=3 each group) using the miRVana miRNA isolation kit (#AM1560, Ambion, Thermo Fisher Scientific, Waltham, MA, USA) in accordance to their manuals ...
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bioRxiv - Molecular Biology 2023Quote: ... qPCR of the cDNA was performed using a primer set for the N gene of RSV (45) and ACTB (Applied Biosystems, TaqMan Assay ID: Hs99999903_m1) as a housekeeping gene ...
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bioRxiv - Cell Biology 2023Quote: ... Expression constructs were made by LR reaction using the following destination vectors: pDEST15 (for N-terminal GST-fusion in E. coli, cat#11802014, Thermo Fisher Scientific, Waltham, MA, USA) and pcDNA-DEST53 (for N-terminal GFP fusion in mammalian cells ...
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bioRxiv - Cancer Biology 2023Quote: ... Surface CRT was detected using anti- CRT(N) or anti-CRT(Cmut) antibody (1:200 dilution) followed by staining with Alexa Fluor™ 647 conjugated secondary antibody (Invitrogen A21245, 1:2000 dilution). Total CRT in healthy donor platelets was stained with anti-CRT(N ...
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bioRxiv - Neuroscience 2023Quote: ... KSR medium was replaced by N2 medium (N2; DMEM/F12 GlutaMAX + 1X B-27 Plus Supplement + 1X N-2 Supplement + 100 U/ml pen/strep [all: Thermo Fisher Scientific, Waltham, MA, USA]), supplemented with 2i + 3i in 25% increments every two days until day 10 ...
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bioRxiv - Cell Biology 2022Quote: ... The protein band corresponding to Fragment 2 was cut out and subjected to N-terminal sequencing analysis (Matsudaira, 1987) on an automated protein sequencer (#Procise 491 cLc; Applied Biosystems, Foster City, CA, USA) using the standard pulsed-liquid program for PVDF-blotted proteins.
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bioRxiv - Bioengineering 2024Quote: A separate set of ATA samples (n=4 per group) were immersed in optimal cutting temperature compound (OCT; 4585, Thermo Fisher Scientific; Waltham, MA, USA) immediately after excision and frozen at 80◦C for fluorescent immunostaining ...
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bioRxiv - Cell Biology 2024Quote: ... Silencer® Select N°1 negative control siRNA or predesigned Silencer® Select siRNAs for ZNF827 (s45694, s45695, s45696) (Thermo Fisher Scientific, Waltham, MA, USA) were used for the transfection process ...
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bioRxiv - Systems Biology 2019Quote: ... The column was washed for a further 3 min with 98% acetonitrile (Optima grade, Fisher Scientific) with 0.1% formic acid and finally equilibrated with 0.1% B for 1.5 min ...
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bioRxiv - Neuroscience 2022Quote: ... Samples were denatured at 98°C for 15 min in 1x SDS sample buffer (Life Technologies) and separated via electrophoresis through 12% Tris-glycine polyacrylamide gels (Nupage ...
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bioRxiv - Plant Biology 2022Quote: The stock solutions for the GLVs (Z)-3-hexen-1-ol (Z3-HOL, 98%; Acros Organics) and (Z)-3-hexenyl acetate (Z3-HAC ...
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bioRxiv - Molecular Biology 2020Quote: ... The test was carried out at the optimized temperature determined as described above with four positive controls (1 pg pUC57-N DNA) and four negative controls (ddH2O) in a StepOne™ System (Applied Biosystems, Foster City, CA, USA).
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bioRxiv - Cancer Biology 2019Quote: Treated cell-pellets were lysed and digested using the commercially available Pierce™ Mass Spec Sample Prep Kit for Cultured Cells (Thermo Fisher Scientific, P/N 84840) following manufacturer’s instructions ...
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bioRxiv - Physiology 2020Quote: All 7 gills from each individual shrimp (5 ≤ N ≤ 8) were dissected under a magnifying lens with micro-scissors and homogenized in TRIzol reagent (Life Technologies, Thermo Fisher Scientific, Waltham, MA, USA) (1 ...
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bioRxiv - Immunology 2022Quote: RNA was extracted from BALB/cByJ x C57BL/6J F1 hybrid dissected spleens (n = 4) preserved in RNA later (Thermofisher, Cat. No. AM7020; Waltham, MA, USA). Briefly ...
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bioRxiv - Bioengineering 2022Quote: Untargeted lipidomics profiling (n = 3) was performed via high-resolution liquid chromatography with tandem mass spectrometry (Thermo Scientific QExactive Plus/HF Orbitrap HR-LC-MS/MS) at the Mass Spectrometry Core at Beth Israel Deaconess Medical Center in Boston ...
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bioRxiv - Systems Biology 2020Quote: ... Figure S5 shows the knockdown efficiency of siRNA transfections in each cell line with GAPDH siRNA compared to no siRNA (just N-TER transfection reagent) and negative control siRNA by Western blot (GAPDH antibody: Cell Signaling Technology, Cat#2118; Tubulin antibody: Thermo Fisher Scientific, Cat#62204). Experiments were performed 24hr after transfection.
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bioRxiv - Pathology 2023Quote: ... Xcc 8004 and Xe 85-10 strains were synthesized as gene fragments with appropriate BpiI sites (GeneArt gene synthesis by Thermo Scientific; codon-optimized for N. benthamiana) and subcloned as level 0 CDS1ns modules (in pAGM1287 ...
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bioRxiv - Physiology 2023Quote: ... The tissue or whole body samples were homogenized in 1 N HCl (100 µl per 10 tissues or whole body) manually using plastic pestles (Thermo Fisher Scientific Inc., Waltham, MA, USA). To enhance homogenization and solubilization ...
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bioRxiv - Neuroscience 2023Quote: ... pellets were reconstituted in 50[µl of 0.1[N NaOH and kept for protein quantification using a BCA protein assay kit (Thermo Scientific Pierce BCA Protein Assay Kit, PI23227). Dialysate concentrations are expressed as ng/ml and tissular concentrations as ng/mg of total proteins.
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bioRxiv - Microbiology 2020Quote: ... DNA samples containing the extension products were added to Hi-Di formamide (Applied Biosystems, Foster City, CA). The mixture was incubated for 5 min at 95°C ...
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bioRxiv - Molecular Biology 2019Quote: ... 20 ng of poly(dI-dC) were used for 100 ng of recombinant ERα (ThermoFisher, RP-310) and 1 µg for 10 µg of nuclear extracts ...
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bioRxiv - Cell Biology 2019Quote: Laurdan (6-dodecanoyl-2-dimethylaminonaphthalene; product D250) and di-4-ANEPPDHQ (product D36802) were purchased from ThermoFisher. Methyl-β-cyclodextrin (product C4555 ...
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bioRxiv - Immunology 2019Quote: ... FACS-Gal analysis was performed using warm hypotonic loading of fluorescein di β-D-galactopyranoside (Molecular Probes) on single cells as described 52 followed by immunophenotyping using relevant surface antigens as defined in Table S1 ...
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bioRxiv - Cancer Biology 2019Quote: ... then labelled with 1,1’-di-octa-decyl-3,3,3’,3’-tetra-methyl-indo-carbo-cya-nine perchlorate (DiI, ThermoFisher) and finally resuspended in a buffer containing 5% FBS in PBS ...
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bioRxiv - Physiology 2020Quote: ... DI/LC-MS/MS was done on an API4000 Qtrap® tandem mass spectrometry instrument (Applied Biosystems/MDS Analytical Technologies ...
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bioRxiv - Molecular Biology 2021Quote: ... and resuspended in Hi-Di formamide supplemented with R0X350 dye standards (1:8 ROXF) (Thermo Fisher Scientific). 1:3 and 1:15 dilutions of the samples in ROXF were analyzed by capillary electrophoresis (ELIM Biopharmaceuticals).
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bioRxiv - Cancer Biology 2022Quote: ... in the reverse phase using a preconcentration column (75 μm DI × 2 cm, 3 μm, Thermo Scientific) and an analytical column (Acclaim PepMap C18 ...
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bioRxiv - Cancer Biology 2022Quote: ... then disaggregated and labelled with 1,1’-di-octa-decyl-3,3,3’,3’-tetra-methyl-indo-carbo-cya-nine perchlorate (DiI, ThermoFisher) and finally resuspended in a buffer containing 5% FBS in PBS ...
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bioRxiv - Pathology 2023Quote: ... The c-di-GMP samples were separated by using an Syncronis C18 column (Thermo Fisher Scientific, USA) fitted with a 100- by 2.1 mm guard column with a flow rate of 0.2 ml/min ...
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bioRxiv - Biochemistry 2023Quote: ... Grids prepared with DI or DE substrates were loaded into a Talos Arctica electron microscope (Thermo Scientific) operated at 200 kV for automated data collection using Leginon 74 ...
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bioRxiv - Biochemistry 2023Quote: ... Samples were heated from 25-98°C in a QuantStudio Flex 12 RT-PCR machine (ThermoFisher Scientific). Measurements of fluorescence emission at 570nm were followed for each 0.3°C increment ...
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bioRxiv - Cancer Biology 2024Quote: ... heat-induced (98°C) antigen retrieval was performed with a citrate buffer (pH 6.0) (Thermo Fisher Scientific), and slides were incubated with Triton X-100 0.25% in PBS for 5 minutes ...
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bioRxiv - Molecular Biology 2021Quote: The human HCT 116 colon cancer cell line was kindly provided to us by the Johns Hopkins Sidney Kimmel Comprehensive Cancer Center (Baltimore, USA) and cultivated in McCoy’s medium (Gibco™, Thermo Fisher Scientific Inc., cat n°22330-021). K-562 (ATCC ...
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bioRxiv - Neuroscience 2023Quote: ... The original INNOTEST kit plate was replaced with a 96-well MicroWell™ MaxiSorp™ flat bottom plate coated with V5 antibody (Invitrogen P/N-0705; 1:10000) in carbonate buffer (NaHCO3 0.1 mM ...
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bioRxiv - Microbiology 2023Quote: ... The template for generating homology-directed repair DNA for gene tagging was constructed by inserting a P2A viral skip peptide in frame with a downstream blasticidin-S deaminase (BSD) or puromycin N-acetyl-transferase (puro) and TOPO cloned into a pCR4 backbone (Thermo Fisher, Waltham, MA, United States of America) 51 ...
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bioRxiv - Genetics 2020Quote: ... Germany) 1 μl of each PCR product was edited with Hi-Di Formamide (20 μl, ThermoFisher, Massachusetts, USA) and Gene Scan 500 ROX dye Size Standard (0.5 μl ...
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bioRxiv - Biophysics 2019Quote: ... 4-(2-(6-(dibutylamino)-2-naphthalenyl)ethenyl)-1-(3-sulfopropyl)-,hydroxide (di-4-ANEPPS) was purchased from Invitrogen. It was dissolved in ethanol and added to the dried lipid film at a 12:1 lipid:probe molar ratio ...
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bioRxiv - Genetics 2022Quote: ... can be measured in live cells by using 5-(pentafluorobenzoylamino) fluorescein Di-β-D-glucopyranoside (PFB-FDGlu; ThermoFisher) as a substrate that is taken up into acidic vesicles only ...
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bioRxiv - Developmental Biology 2019Quote: ... single cell suspensions were mixed with 100 μL prewarmed 2 mM fluorescein di-β-D-galactopyranoside (FDG) (Invitrogen) diluted in water and then incubated in a 37°C water bath for 1-2 minutes to permit substrate uptake ...
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bioRxiv - Cell Biology 2021Quote: ... Coverslips were rinsed 2 x in DI water before mounting onto 1 mm-thick glass slides (Thermo Scientific) using anti-fade mounting medium (Dako).
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bioRxiv - Immunology 2023Quote: ... Sucrose meals were created by passing a solution of DI water and UltraPure sucrose (Life Technologies, Carlsbad, CA) through a 0.2 μm filter ...
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bioRxiv - Cell Biology 2024Quote: ... 200 µl liquid PTNT medium containing 50 µM Di-4-ANEPPDHQ (ThermoFisher; stock solution 10 mM in EtOH) were added and plates were incubated for another 15 min at RT in the dark ...
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bioRxiv - Plant Biology 2020Quote: ... The limit and efficiency of each primer pair of both target and reference gene was allowed to measure accurate comparison of gene expression using the 2-ΔΔCT method for relative quantification (the Applied Biosystems User Bulletin No. 2 (P/N 4303859)) (Livak Schmittgen ...
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bioRxiv - Biochemistry 2020Quote: ... The mixture was then brought to 40 °C and 9 g of tetraethyl orthosilicate (98%, ACROS Organics #1577812500) was added ...
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bioRxiv - Biochemistry 2020Quote: ... the magnetic beads were resuspended in 10.0625 μL ROX/Hi-Di (0.0625 μL of ROX 350 ladder, Applied Biosystems, in 10 μL of Hi-Di formamide ...
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bioRxiv - Biochemistry 2020Quote: ... the magnetic beads were resuspended in 10.0625 μL ROX/Hi-Di (0.0625 μL of ROX 350 ladder [Applied Biosystems] in 10 μL of Hi-Di formamide [Applied Biosystems] ...
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bioRxiv - Cancer Biology 2020Quote: ... 20 fmol of labeled probe was bound to 0.55 pmol MYC:MAX or 0.06 pmol MAX:MAX dimers in the presence of 20 ng poly(dI-dC) (Thermo Fisher 20148E) in binding reaction buffer (15 mM Tris pH 7.9 ...