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Citations for Thermo Fisher :
1601 - 1650 of 1930 citations for N Nitroso Di N Propylamine D14 98% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2023Quote: ... both diluted 1:50 in DMEM/F-12 or with 20 ug/mL recombinant truncated vitronectin (VTN-N) (Thermo Fischer Scientific, A31804) diluted in DMEM/F-12 (Gibco, 21331-020) for 1 hr at room temperature ...
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bioRxiv - Cancer Biology 2023Quote: ... Total RNA was isolated from MG63 lysates (n = 2 per treatment condition) using the PureLink RNA Mini kit (12183018A, Thermo Fisher Scientific) with DNase set (12185010 ...
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bioRxiv - Cell Biology 2023Quote: Liquid chromatography separation was performed at a flow rate of 300 nL/mL on a C18 pre-column (Acclaim PepMapTM 100, 75 μm×2 cm, nanoViper, P/N 164946, ThermoFisher Scientific Incorporation) followed by analytical column (Acclaim PepMapTM RSLC C18 ...
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bioRxiv - Cancer Biology 2023Quote: Wild-type and mutant (C139A) USP39 with a Flag-tag at its N-terminal were cloned into the pLenti6/V5 lentiviral vector (Invitrogen, California, USA). Specific shRNA oligonucleotides targeting USP39 were cloned into the pLL3.7 lentiviral vector (Addgene) ...
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bioRxiv - Genetics 2023Quote: ... The optimized and minimum required protocol for organoid hepatic isolation consisted of rinsing the fresh tissue in Phosphate Buffered Saline (PBS)/N-acetylcysteine (NAC) once and then transferring it to a tube filled with Advanced DMEM/F12 (Gibco; 12634-010), mincing the tissue into small fragments ...
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bioRxiv - Evolutionary Biology 2023Quote: ... into the packaging 293T cells (with molar ratio for Luc-T20: N: M/E: S as 3: 1: 1: 1) with LipofectamineTM 2000 (Thermo Fisher Scientific). At 24 and 48 hours post-transfection ...
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bioRxiv - Cell Biology 2023Quote: ... at a density of 107 cells in 19 ml of medium for dual-proteomics or at 1.5 x 104 cells per well in 96-well plates (Nunc, cat n° 164590) in 0.2 ml of medium for MTT ...
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bioRxiv - Genomics 2023Quote: ... coated dishes in N2 medium (high-glucose DMEM, 1% N-2, 2% B-27 and 1% penicillin/streptomycin, from Thermo Fisher Scientific) at a density of 2×105 cells/cm2 ...
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bioRxiv - Immunology 2022Quote: ... which was created via the in vitro T7-DNA-dependent RNA transcription of a linearized DNA molecule containing the target region of the N gene full-length by using MEGAscript T7 Transcription Kit (ThermoFisher, Cat# AM1334), and purified with MEGAclear Transcription Clean-Up Kit (ThermoFisher ...
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bioRxiv - Plant Biology 2023Quote: ... The roots were lyophilized in vacuo and analyzed for total N and 15N content using elemental analysis–isotope ratio mass spectrometry (Flash EA1112-DELTA V PLUS ConFlo III system, Thermo Fisher Scientific).
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bioRxiv - Microbiology 2023Quote: ... Infectious titers were determined by focus-forming unit assay whereby foci of infection were measured using anti-nucleocapsid (N) antibody (Invitrogen; MA1-7403). Where indicated ...
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bioRxiv - Cell Biology 2024Quote: ... Glucose was traced using 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (2-NBDG, Invitrogen, ThermoFisher, cat. #N13195) combined with 0,57 mg/mL 40kDa tetramethyl-rhodamine Dextran (Invitrogen ...
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bioRxiv - Neuroscience 2024Quote: A total of 12 WT and pink1 KO mice (N = 3 per sex/genotype) at age of three months were anesthetized and intracardially perfused with cold PBS (Invitrogen, 14190-235). Brains were removed and immediately flash-frozen in liquid nitrogen ...
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bioRxiv - Neuroscience 2024Quote: ... and granulocyte-macrophage colony-stimulating factor (GM-CSF, 10 ng/ml) to DMEM/F12 (HyClone™, SH30023.01) containing N-2 supplement (Gibco™, 17502048). This protocol facilitated the maturation process of the PMPs into microglia.
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bioRxiv - Molecular Biology 2024Quote: ... level was measured from the plasma of representative subsets of the groups (n=16) using an automated analyzer (Indiko Plus, Thermo Fisher, US).
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bioRxiv - Microbiology 2023Quote: ... The chip was activated with a freshly prepared solution of 130 mM 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) (Pierce PG82079) and 33 mM N-hydroxysulfosuccinimide (Sulfo-NHS) (ThermoFisher Scientific 24510) in 0.1 M MES pH 5.5 using the SFC ...
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bioRxiv - Microbiology 2023Quote: ... The chip was activated with a freshly prepared solution of 130 mM 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride (EDC) (Pierce PG82079) and 33 mM N-hydroxysulfosuccinimide (Sulfo-NHS) (ThermoFisher Scientific 24510) in 0.1 M MES pH 5.5 using the SFC ...
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bioRxiv - Molecular Biology 2023Quote: ... Biotinylation of Htz1(V126C) was carried out using N-[6-(Biotinamido)hexyl]-3’-(2’-pyridyldithio)propionamide (HPDP-Biotin) (Thermo Fisher cat. # 21341), which has a pyridyl disulfide moiety ...
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bioRxiv - Biochemistry 2023Quote: ... Dried samples were redissolved with 300 μL of 0.1% TFA in H2O and further fractionated using high-pH reversed-phase peptide fractionation kits (ThermoFisher, P/N 84868) following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... the co-localization of bacterial biomass and necrotic tissue was assessed by a pathologist in samples with high bacterial biomass (n = 21) using an EVOS M7000 microscope (Thermofisher MA, USA).
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bioRxiv - Plant Biology 2023Quote: ... L−1 of 3’-biotin-labeled primer probe purified by high-pressure liquid chromatography (HPLC) for 4-12 h at 42°C in Hybaid Shake ‘n’ Stack (Thermo Fisher Scientific) with gentle rotation ...
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bioRxiv - Microbiology 2024Quote: ... Peptides were loaded on a trapping column and eluted over a 75μm x 50cm analytical column (Thermo Fisher P/N ES-803) at 300 nL/min using a 1hr reverse phase gradient ...
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bioRxiv - Genetics 2023Quote: ... Total RNA was extracted from n = 3–4 biological replicates per sample using the PureLink RNA Mini Kit (Invitrogen, Waltham, MA, USA), and sample quality was evaluated using a TapeStation ...
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bioRxiv - Immunology 2023Quote: ... and activated with a mixture of 400 mM 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride and 100 mM N-hydroxysuccinimide (Thermo Fisher Scientific). The activated chip was lawned with goat anti-human IgG Fc (50 µg/mL ...
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bioRxiv - Immunology 2023Quote: ... and activated with a mixture of 400 mM 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride and 100 mM N-hydroxysuccinimide (Thermo Fisher Scientific). The activated chip was coupled directly to mAbs diluted to 10 µg/mL in 10 mM sodium acetate (pH 4.5 ...
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bioRxiv - Neuroscience 2024Quote: ... NPC were plated on poly- L-ornithine/laminin-coated flasks at 50’000 cells/cm2 in2µg/ml laminin-supplemented neural expansion medium (DMEM/F-12 + Glutamax, Gibco®; 1x N-2 supplement, Gibco®; 1x B27 supplement without vitamin A, Gibco® ...
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bioRxiv - Plant Biology 2024Quote: ... Contents of C and N were determined from 5 mg of tissue powder on a Flash 2000 Elemental Analyzer (Thermo Fisher Scientific), and contents of S were determined from 20 mg of tissue powder mixed with 5 mg of tungsten trioxide on an elemental PYRO cube analyzer (Elementar) ...
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bioRxiv - Neuroscience 2024Quote: ... Samples were first concentrated over an Acclaim PepMap trap column (5.0 µm C18-coated particles, 0.5 cm * 300 µm ID, ThermoFisher Scientific P/N 160454), then bound onto a PepSep XTREME column (1.5 µm C18-coated particles ...
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bioRxiv - Bioengineering 2024Quote: ... which in turns intercalates with nucleic acids and emits red fluorescence detectable by microscopy.38 Frozen tissue sections from ATA samples (n=4 per group) were stained with DHE (10µM; D1168, Invitrogen; Carlsbad, CA, USA) in a dark humidified incubator at 37◦C for 20 minutes ...
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bioRxiv - Biochemistry 2024Quote: ... hamster (Syrian Hamster Isoform X1 – UniProt: A0A1U8BWQ2) and macaque (Macaque – UniProt: F6SVR2) with an N-terminal cMYC-tag were ordered to GeneArt (Thermo Fisher Scientific) and cloned into a phCMV backbone (GeneBank ...
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bioRxiv - Biochemistry 2024Quote: ... with a replacement in the N-terminal domain loop 8 (139CQKRDGPH146 to 139AEAG142, designated as rA3GR8) was constructed in the pET-sumo vector (Thermo Fisher Scientific). This construct generated a SUMO fusion with an N-terminal 6xHis tag and a PreScission protease cleavage site ...
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bioRxiv - Biochemistry 2024Quote: ... and cloned into a pRK5-derived plasmid with a C- or N-terminal FLAG tag using the gateway vector conversion system (Invitrogen, cat # 11828029). All gene constructs were verified by DNA sequencing.
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bioRxiv - Cell Biology 2024Quote: ... cells were seeded onto coated 35 mm glass bottom dishes with a 20 mm glass aperture (Fisher Scientific D35-20-1.5-N). HEK293A cells were seeded onto dishes that had been coated with 10 µg ECL cell attachment matrix (Sigma 08-110 ...
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bioRxiv - Cell Biology 2024Quote: ... Antibody-mediated inhibition of hNR-EVs was performed with a commercial antibody that recognizes an extracellular topological domain of PLP1 at its N terminus (AA 36 to 85, Invitrogen, PA5-40788) incubating hNR-EVs with anti-PLP1 1.25 ug/ml for 15 min ...
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bioRxiv - Immunology 2024Quote: ... and SB28 RRV-IRF8 (100% transduced) cells were plated at 1x105 cells (n=3 per time point) and counted (Thermofisher Countess 3)at 24 and 48 hours post-seeding ...
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bioRxiv - Microbiology 2024Quote: ... and also cultured16 the fluid by spread-plating 20 microliters onto a pre-reduced tryptic soy 5% defibrinated sheep blood agar (80% N–10% H– 10% CO2, at 37°C; Thermo Fisher Scientific) using a variable-atmosphere anaerobic Whitley workstation A85 (540 plate capacity ...
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bioRxiv - Biochemistry 2024Quote: ... Dried samples were redissolved with 300 μL of 0.1% TFA in H2O and further fractionated using high-pH reversed-phase peptide fractionation kits (ThermoFisher, P/N 84868) following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2020Quote: ... 6-ketocholestanol or phloretin cells were incubated with di-8-ANEPPS (Thermo Fisher, D3167) at a final concentration of 2 μM on ice for 20 minutes (33 ...
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bioRxiv - Bioengineering 2022Quote: ... where 50 μL of 0.2 mM Fluorescein-Di-β-D-Galactopyranoside (FDG; Life Technologies) is added as the fluorogenic substrate ...
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bioRxiv - Physiology 2019Quote: TT’s were visualized by staining with di-4-ANEPPS (4 µmol/l. Invitrogen, UK) as described in detail previously 8,32,70 ...
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bioRxiv - Bioengineering 2019Quote: Droplets were created with deionized (DI) water (Type II, Harleco; Fisher Scientific, Hampton, NH) tinted with either yellow or green dye (Spice Supreme ...
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bioRxiv - Synthetic Biology 2020Quote: ... 3.3 nM of 5-chloromethylfluoresecein di-beta-D-galactopyranoside (CMFDG) (Invitrogen, Waltham, MA, USA) was added to the reaction solution ...
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bioRxiv - Physiology 2022Quote: ... UUT explants were loaded with the membrane voltage sensitive dye di-4-ANEPPS (Invitrogen) that exhibits depolarizing voltage dependent shifts in green emission (increased intensity ...
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bioRxiv - Cell Biology 2024Quote: ... When using the β-galactosidase stain C12FDG (5-Dodecanoylaminofluorescein Di-β-D-Galactopyranoside, ThermoFisher), cells were incubated with C12FDG dye for 2 hoursLat 37°C and washed twice before staining with surface marker antibodies ...
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bioRxiv - Biochemistry 2021Quote: The 17-mer peptide SDEVTVETTSVFRADFL was produced with a purity >98% by Thermo Fisher Scientific.
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bioRxiv - Neuroscience 2022Quote: ... the spent media was replaced by Neural Induction Medium containing 98% Neurobasal medium (Gibco) and 2% neural induction supplement (Gibco ...
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bioRxiv - Neuroscience 2022Quote: ... The cDNA was synthetized with 98 ng RNA using SuperScript III Reverse Transcriptase (Invitrogen) and random hexamer primers (Thermofisher Scientific) ...
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bioRxiv - Genetics 2021Quote: ... using a Leica M205C stereomicroscope (190-240X magnification) and collected directly into sterile 0.2 mL PCR flat-cap tubes containing Prep-n-GoTM Lysis buffer (ThermoFisher Scientific, Carlsbad, CA, USA). This was done via a sterile tungsten needle and water-soluble 3MTM adhesive ...
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bioRxiv - Microbiology 2022Quote: ... and expression clones with N-terminal fusion tags were produced simply by Gateway cloning (Gateway™ LR Clonase™ II Enzyme mix, Invitrogen, 11791020). The Lenti-NSP2 was constructed by using the destination vector ...
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bioRxiv - Neuroscience 2021Quote: ... and for bank vole cells NBM complete was switched to DMEM complete supplemented with 1% N-2 supplement (Gibco™ Thermo Fisher, USA). Mouse or bank vole glia cells were plated at 1×105 or 5×104 cells/well ...