Labshake search
Citations for Thermo Fisher :
1751 - 1800 of 1930 citations for N Nitroso Di N Propylamine D14 98% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Developmental Biology 2021Quote: ... for amplification of 100ng of cDNA template from N/D/S cells either treated with MS351 or vehicle using Power SYBR Green Master Mix (Thermo Fisher Scientific Cat. 4368577) qRT PCR protocol14 ...
-
bioRxiv - Cancer Biology 2022Quote: ... with or without N-ethyl-N-nitrourea 10μg/ml or 50μg/ml and treated 2h with 1mg/ml Collagenase type IV (Gibco by life technologies ref 17104-019).
-
bioRxiv - Neuroscience 2023Quote: ... for 2 h RT and incubated o/n with a primary antibody cocktail containing anti-GFP antibody (polyclonal chicken anti-GFP, 1:2000; #A10262, AB_2534023; Thermo Fisher Scientific, Rockford, IL, USA), anti-TH antibody (TH polyclonal rabbit anti-mouse ...
-
bioRxiv - Plant Biology 2022Quote: ... were collected 2 days post-infiltration and ground to a homogeneous powder in liquid nitrogen and resuspended in 1 mL of protein extraction buffer (1× PBS, 1% n-dodecyl- β-d-maltoside or DDM (Invitrogen, Carlsbad, CA, USA), and 0.1 tablet cOmplete™ protease inhibitor cocktail (Sigma-Aldrich ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... the cell layers are examined for cell morphology using a phase contrast microscope washed with media and then 10 uM of 2-(N-[7-nitrobenz-2-oxa-1, 3-diazol-4-yl] amino)-2-deoxyglucose (2-NBDG) (Molecular Probes-Invitrogen, CA, USA) was used to assess glucose uptake in the presence or absence of 100 nM insulin as the initiating step and incubated for 20 or 30 minutes ...
-
Development of monoclonal antibody-based blocking ELISA for detecting SARS-CoV-2 exposure in animalsbioRxiv - Microbiology 2023Quote: ... Cell culture supernatants containing specific anti-N mAb were harvested and concentrated using Pierce™ Saturated Ammonium Sulfate Solution (Thermo Fisher Scientific, Waltham, MA). Biotinylation of the mAb was performed using a Biotin Conjugation Kit by following the manufacturer’s instruction (Abcam ...
-
bioRxiv - Cancer Biology 2023Quote: Bulk RNA was isolated from TA muscles of five mice from each of the four treatment groups (n=20) using Trizol (ThermoFisher Scientific, Waltham, MA, USA) and established methods.32 A NanoDrop spectrophotometer was used to determine RNA purity ...
-
bioRxiv - Biophysics 2023Quote: Human neuroblastoma cell line (SK-N-BE) stored at -80°C in freezing medium (RPMI Gibco® supplemented with 20% FBS Gibco and 10% DMSO), were quickly defrosted at 37°C ...
-
bioRxiv - Cell Biology 2022Quote: ... 1:200 O/N at 4 °C) and Alexa Fluor 594-conjugated anti-mouse IgG antibody (#A11005, Invitrogen, 1:2000 for 2 hours RT). Nuclei staining was done in 0.5 μg/mL Hoechst 33324 (Sigma-Aldrich ...
-
bioRxiv - Immunology 2023Quote: ... Sequence-verified Cas9-gRNA-Puro plasmids and pMA donor plasmid were co-transfected into in-house C57BL6/N 6.0 ESC using Lipofectamine 2000 (Thermo Fisher Scientific, Waltham, MA, USA) prior to selection with 2 μg/ml puromycin for 48 hours ...
-
bioRxiv - Biochemistry 2023Quote: ... as three full experimental technical replicates (n=54) with ∼100 ng of peptides injected for each chromatographic run using a Dionex UltiMate 3000 uHPLC system (Thermo Fisher Scientific, Bremen, Germany). Solvent A was 1% CH3CN in 0.1% (v/v ...
-
bioRxiv - Plant Biology 2023Quote: ... 5,000 μg of dry powder was placed in tin capsules to quantify the total N and C concentrations using the FLASH 2000 Organic Elemental Analyzer (Thermo Fisher Scientific Villebon, France) and the 15N enrichment using the Delta V Advantage isotope ratio mass spectrometer (Thermo Fisher Scientific ...
-
bioRxiv - Cell Biology 2023Quote: A live/dead assay on the cell-laden GelMa constructs (n=6 per condition) was performed using a LIVE/DEADTM Cell Imaging kit (Invitrogen, Waltham, Massachusetts, United States) 144 h after Gel synthesis ...
-
bioRxiv - Cancer Biology 2023Quote: ... Whole-cell lysates were prepared from 5x106 cells treated with N-acetylcysteine (MilliporeSigma A7250) for 4 hours followed by stimulation/oxidation with 1 mM hydrogen peroxide (Fisher Scientific Canada, Mississauga ON) for 5 minutes ...
-
bioRxiv - Plant Biology 2023Quote: ... were collected 2 days post-infiltration and ground to a homogeneous powder in liquid nitrogen and resuspended in 1 mL of protein extraction buffer (1× PBS, 1% n-dodecyl β-d-maltoside or DDM (Invitrogen, Carlsbad, CA, USA), and 0.1 tablet cOmplete™ protease inhibitor cocktail (Sigma-Aldrich ...
-
bioRxiv - Microbiology 2023Quote: ... determined by preparing a slurry with 1 part soil to 2 parts deionized water and measuring (n=3) with an Orion Star A111 pH Meter (Thermo Scientific, Waltham, MA, USA), was 5.87 ± 0.42 in November 2020 and 6.28 ± 0.07 in April 2021.
-
bioRxiv - Biochemistry 2024Quote: ... or 5 × 103 cells/mL (N. gruberi) in 100 µL of the indicated medium in white TC-treated 96-well plates (Thermo Scientific Nunc Microwell 136101). For assays under standard growth conditions (“+ glc”) ...
-
bioRxiv - Biophysics 2019Quote: ... Samples were heated at 98°C and loaded onto 4-12% Bis-Tris gels (Invitrogen). Protein bands were then detected by Coomassie blue staining ...
-
bioRxiv - Cell Biology 2019Quote: ... at 98°C for 20 min using a Lab Vision Pretreatment Module™ (Thermo Scientific). After permeabilization with PBS/0.3% Triton-X100 for 5 min ...
-
bioRxiv - Microbiology 2022Quote: ... Ammonium acetate (≥98%, Optima LC/MS) was purchased from Fisher Scientific (Fair Lawn, NJ, USA). Water was purified by a Milli-Q system (resistivity 18.2 MΩ cm at 25°C ...
-
bioRxiv - Plant Biology 2020Quote: ... 0.02 mg/mL of poly (deoxyinosinic-deoxycytidylic) sodium salt [poly (dI-dC)] (Thermo Scientific, Cat. #20148E), 1 mM DTT and 10% glycerol ...
-
bioRxiv - Molecular Biology 2021Quote: ... Completed reactions were ethanol precipitated and re-suspended in deionized formamide (Hi-Di formamide, Thermo Fisher) with the G-ladders ...
-
bioRxiv - Cell Biology 2022Quote: ... Hearts were stained with voltage sensitive indicator di-4-ANNEPS (Thermo Fisher Scientific, Waltham, MA, USA), using 20 μL of stock solution (1 mg/mL of DMSO ...
-
bioRxiv - Genetics 2019Quote: ... Each gel included a migration standard comprised of Di-I labeled human LDL (L3482, ThermoFisher Scientific) that was diluted in cryoprotectant and stored in frozen aliquots (see recipes) ...
-
bioRxiv - Bioengineering 2019Quote: ... devices were washed twice with DI H2O and then stained with 1% silver nitrate (Acros Organics) in DI H2O under a UV lamp for 15 minutes ...
-
bioRxiv - Bioengineering 2019Quote: ... Devices were washed twice with DI H2O and then incubated in 5% sodium thiosulfate (Acros Organics) in DI H2O for 5 minutes ...
-
bioRxiv - Bioengineering 2022Quote: ... hydrogels were rehydrated with 1 mg ml-1 Sulfo-SANPAH diluted in DI water (22589, ThermoFisher) and irradiated with collimated UV light (λ = 365 nm ...
-
bioRxiv - Bioengineering 2022Quote: ... and DI water was degassed under vacuum in an ultrasonic cleaning bath (15-337-411, ThermoFisher). SDS ...
-
bioRxiv - Genomics 2023Quote: ... for 2 h and with 33 nM C12FDG (5-Dodecanoylaminofluorescein Di-β-D-Galactopyranoside; ThermoFisher D2893) for 1 h ...
-
bioRxiv - Physiology 2023Quote: ... were incubated for 20 minutes with 5 µM of Di-3-ANEPPDHQ (Thermo Fisher Scientific, USA) membrane dye ...
-
bioRxiv - Neuroscience 2024Quote: ... carbogen-bubbled ACSF with the addition of 15uL of 5mg/mL Di-4-ANNEPS (Thermofisher #D1199) in high purity ethyl alcohol (EtOh) ...
-
bioRxiv - Physiology 2023Quote: ... we stained the preparations by perfusing with 10 μM di-4-ANEPPS (Molecular Probes, Eugene, OR) for 10 min ...
-
bioRxiv - Microbiology 2020Quote: ... and resuspended in 0.1 ml of PBS supplemented with the lipophilic styryl membrane dye N-(3-triethylammoniumprpyl)-4-(p-diethylaminophenyl-hexatrienyl) pyridinium dibromide (FM4-64, Molecular Probes, Invitrogen; 10 μg.ml-1) (Pogliano et al. ...
-
bioRxiv - Neuroscience 2020Quote: ... were quantified in autaptic neuronal cultures using N-(3-triethylammoniumpropyl)-4-(4-(dibutyl amino) styryl) pyridinium dibromide (FM1-43, Thermo Fisher Scientific, Waltham, MA, USA), similarly as in our previous report (Kawano et al. ...
-
bioRxiv - Molecular Biology 2021Quote: ... the medium was replaced with 5 ml fresh medium and 50 or 100 μM 12-azidodo-decanoate (azidomyristate) (Click-IT™ Myristic Acid, Azide, ThermoFisher Scientific, Cat n° C10268) in dimethyl sulfoxide (DMSO ...
-
bioRxiv - Microbiology 2019Quote: ... with different spectral signatures selected for the detection of PfHRP2 and PAN-pLDH were washed with distilled water and activated with Sulfo-NHS (N-hydroxysulfosuccinimide) and EDC (1-ethyl-3-[3-dimethylaminopropyl] carbodiimide hydrochloride) (Pierce, Thermo Fisher Scientific Inc., Rockford, IL), both at 50 mg/mL ...
-
bioRxiv - Cancer Biology 2019Quote: ... Individual samples were then labeled with isobaric tags using commercially available Tandem Mass Tag™ 6-plex (TMTsixplex™) (Thermo Fisher Scientific, P/N 90061) or TMT11plex (TMT11plex™ ...
-
bioRxiv - Cell Biology 2019Quote: ... 2,7-Dichlorodihydrofluorescein diacetate (DCFH-DA),3,3’-dihexyloxacarbocyanine iodide [DiOC6(3)] and N-[4-[6-[(acetyloxy)methoxy]-2,7-difluoro-3-oxo-3H-xanthen-9-yl]-2-[2-[2-[bis[2-[(acetyloxy)methoxy]-2-oxoethyl]amino]-5-methylphenoxy]ethoxy]phenyl]-N-[2-[(acetyloxy)methoxy]-2-oxoethyl]-,(acetyloxy)methyl ester (Fluo-4 AM) were purchased from Molecular Probes (Invitrogen, Eugene, OR, USA). Agarose was obtained from Lonza (Walkersville ...
-
bioRxiv - Bioengineering 2021Quote: Each sample (n=5) was placed in a 2 mL centrifuge tube containing 320 μL of collagenase II (Gibco, Thermo Fisher Scientific Inc., MA, USA) solution at 37 °C ...
-
bioRxiv - Microbiology 2021Quote: ... All CAMP (Christie-Atkins-Munch-Peterson)-positive [22] bacterial isolates (n=58) were confirmed as GBS using a slide latex agglutination test (Streptex Latex Agglutination Test, ThermoFisher Scientific Inc., Waltham, MA, USA). GBS isolates were subcultured on blood agar to assess purity and inoculated on stab agars (SVA ...
-
bioRxiv - Neuroscience 2022Quote: ... consisting of DMEM/F12 GlutaMAX + 1X B-27 Plus Supplement + 1X N-2 Supplement + 100 U/ml 1% penicillin/ streptomycin (pen/strep; all Thermo Fisher Scientific, Waltham, MA, USA) + 20 ng/ml BDNF + 20 ng/ml GDNF + 20 ng/ml NGFb (all Peprotech ...
-
bioRxiv - Cancer Biology 2022Quote: ... collected as single cell suspensions using cell strainer and resuspended in sphere formation medium which was composed of 1:1 DMEM and F-12 nutrient mix supplemented with B-27 and N-2 (Thermo Fisher Scientific, Waltham, MA, USA). 1,000 cells were plated in each well of ultra-low attachment 6-well plates (Corning ...
-
bioRxiv - Genetics 2022Quote: ... Over the course of the following 4 days, the colonies were gradually transitioned to neural induction medium (NIM) (consisting of DMEM/F12, 1% N-2 supplement (Fisher Scientific, Cat #: 17-502-048), MEM non-essential amino acids (Fisher Scientific ...
-
bioRxiv - Microbiology 2022Quote: ... per well were distributed in Ibidi® µ-slide 8-well chambered coverslips and stained with 0.8 μM of the membrane specific fluorescent styryl dye N-(3-triethylammoniumpropyl)-4-(6-(4-(diethylamino) phenyl) hexatrienyl) pyridinium dibromide (FM4-64; Thermo Fisher Scientific, Waltham, MA, USA) in the presence of 0 μM (control) ...
-
bioRxiv - Microbiology 2021Quote: ... Mtb-SC and Mtb-AG-infected rabbit lungs (n=3 per group per time point) was isolated using TRIzol reagent (Life Technologies, Grand Island, NY, USA) as described earlier (5 ...
-
bioRxiv - Cancer Biology 2021Quote: ... RNA from each sample was used to generate amplified and biotinylated sense-strand cDNA from the entire expressed genome according to the Sensation Plus FFPE Amplification and WT Labeling Kit (P/N 703089, Rev.4 Thermo Fisher Scientific Inc., Life Technologies). GeneChip ST Arrays (GeneChip Human Gene 2.1 ST Array Plate ...
-
bioRxiv - Cell Biology 2019Quote: ... methoxy]-2-oxyethyl] amino]-5-methyl-phenoxy] ethoxy]phenyl-N-[2-[(acetyloxy) methoxy]-2-oxyethyl]-(acetyloxy) methyl ester (Fluo-4/AM) were from Molecular Probes (Invitrogen, Eugene, OR, USA). M ...
-
bioRxiv - Biochemistry 2021Quote: ... Enzyme assays were conducted in 0.5 mL containing 50 mM 2-(N-morpholino)ethanesulfonic acid (pH 6) and various concentrations of soluble starch (Acros Organics, No. AC424495000, Morris Plains, NJ). The assays also included 100 mM KCl ...
-
bioRxiv - Molecular Biology 2021Quote: ... and hNAA40 vectors served as templates to generate attB-flanked hNAA40 L(dTIS>CTC) and hNAA40S PCR products suitable for use in a Gateway® BP recombination reaction with a donor vector (pDONR221, Invitrogen, cat n°12536-017) thereby creating an entry clone ...
-
bioRxiv - Plant Biology 2019Quote: ... pollinated stigma was incubated in FM™ 4-64 Dye (N-3-Triethylammoniumpropyl-4-6-4-Diethylamino Phenyl Hexatrienyl Pyridinium Dibromide, Life Technologies T3166, 8.23 μM) for five minutes and subsequently washed in 1/2 Murashige and Skoog basal medium containing 10 % (w/v ...