Labshake search
Citations for Thermo Fisher :
1701 - 1750 of 1930 citations for N Nitroso Di N Propylamine D14 98% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Genomics 2023Quote: ... Cells were then plated onto Geltrex-coated surfaces at 1.5×105 cells/cm2 in the induction medium (DMEM/F12 supplemented with 1× N-2 (Thermo Fisher Scientific, cat# 17502048), 1× non-essential amino acids (Thermo Fisher Scientific ...
-
bioRxiv - Cell Biology 2023Quote: ... and anti-N-WASP (Cell Signalling #4848S; 1:100) followed by Alexa Fluor 568 conjugated secondary antibodies (Invitrogen; 1:1000 in blocking buffer). Actin tails were labeled with Alexa Fluor 488 phalloidin (Invitrogen ...
-
bioRxiv - Microbiology 2023Quote: ... The N concentrations were determined by dry combustion of a 3-4 mg sample with a Flash EA1112 elemental analyzer (Thermo Scientific, Rodano, Italy).
-
bioRxiv - Biochemistry 2022Quote: ... The peptides were separated at a flow rate of 300 nL/minute on C18 pre-column (Acclaim PepMap™ 100, 75 μm□×□2 cm, nanoViper, P/N 164946, ThermoFisher Scientific Incorporation) followed by analytical column (Acclaim PepMap™ RSLC C18 ...
-
bioRxiv - Cell Biology 2022Quote: Total RNA was extracted from the LV myocardium of 2-month-old male WT and Rbm20ΔRS mice (n = 3 per group) using TRIzol Reagent (Life Technologies, Waltham, MA, USA) and treated with DNase I (cat# D9905K ...
-
bioRxiv - Plant Biology 2023Quote: ... Equal amount of protein samples were electrophoresed on 10 % NuPAGE® Bis-Tris Gels in 1X MES [2-(N-morpholino) ethane sulfonic acid] SDS buffer (Invitrogen, Waltham, MA) and transferred to a nitrocellulose membrane (BioRad ...
-
bioRxiv - Molecular Biology 2023Quote: Total protein from muscle tissue samples (n = 6 for EarlyMT and n = 16 for LateMT) was extracted using an extraction buffer containing Tissue extraction Reagent I (FNN0071, Invitrogen, Waltham, Massachusetts, USA), HALT Protease and Phosphatase Inhibitor Cocktail (1:100 ...
-
bioRxiv - Biochemistry 2023Quote: ... Both pDEST-CMV-N-EGFP-wtG3BP2 and pDEST-CMV-N-EGFP-C73SG3BP2 plasmids were amplified and extracted using PureLink™ HiPure Plasmid Maxiprep Kit (Invitrogen™, K210007) before use.
-
bioRxiv - Molecular Biology 2023Quote: ... and mouse (n=6; kept on HFD for 28 weeks) livers were measured with the GeneChipTM miRNA 4.0 Array (Applied Biosystems, Foster City, US).
-
bioRxiv - Neuroscience 2023Quote: ... and maintained in N2B27 media (1:1 of DMEM F12 Glutamax [Thermo Fisher Scientific, 10565018] and Neurobasal media [Thermo Fisher Scientific, 12348017], 1X N-2 (Thermo Fisher Scientific ...
-
bioRxiv - Neuroscience 2024Quote: ... Neuronal differentiation was induced by culturing NGN2-NPC in neural differentiation medium (DMEM/F-12 + Glutamax, Gibco®; 1x N-2 supplement, Gibco®; 1x B27 supplement without vitamin A, Gibco®) supplemented with doxycycline (2µg/ml) ...
-
bioRxiv - Neuroscience 2024Quote: ... Real-time PCRs were performed in technical and independent experiment triplicates (n=3) with Power Sybr Green Master Mix (Life Technologies, Darmstadt, Germany) on a VIIA7 (Life Technologies ...
-
bioRxiv - Molecular Biology 2024Quote: ... 2008) N-terminally tagged with the V5 epitope was synthesized as a DNA string (GeneArt Strings DNA Fragments, Thermo Fisher Scientific, Schwerte, Germany) and cloned into pcDNA3.1 to obtain pcDNA3.1-V5-RHEB-Q64L ...
-
bioRxiv - Cancer Biology 2023Quote: ... Real-time PCR reactions were run in triplicates (n=3) in the 7500 Real-time PCR system by Applied Biosystems (Thermo Fisher Scientific) using power SYBR Green PCR master mix (Applied Biosystems ...
-
bioRxiv - Biochemistry 2024Quote: ... or 5 × 103 cells/mL (N. gruberi) in 100 µL of the indicated medium in white TC-treated 96-well plates (Thermo Scientific Nunc Microwell 136101). For assays under standard growth conditions (“+ glc”) ...
-
Genomic approach for conservation and the sustainable management of endangered species of the AmazonbioRxiv - Genomics 2020Quote: ... One microliter of amplification was mixed with 8.5 μL Hi-Di deionized formamide (Applied Biosystems), and 0.5 μL GeneScan 500 LIZ (Applied Biosystems ...
-
bioRxiv - Biophysics 2020Quote: ... probes were rinsed with DI water 3× and incubated with CellTracker Deep Red (C34565, Invitrogen) at room temperature for 30 minutes ...
-
bioRxiv - Molecular Biology 2020Quote: ... the reaction pellets were dried and resuspended in Hi-Di formamide (Applied Biosystems/Life Technologies).
-
bioRxiv - Molecular Biology 2020Quote: ... the reaction pellets were dried and resuspended in Hi-Di formamide (Applied Biosystems/Life Technologies).
-
bioRxiv - Biophysics 2020Quote: ... Di-4-ANEPPDHQ and Laurdan (6-dodecanyl-2-dimethylaminonaphtalene) were from Molecular Probes (Eugene, OR). L-α-phosphatidylcholine from chicken egg (EggPC) ...
-
bioRxiv - Physiology 2019Quote: ... Each gel included a migration standard of Di-I-labeled human LDL (L3482, ThermoFisher Scientific). Gels were run at 50V for 30 min ...
-
bioRxiv - Evolutionary Biology 2019Quote: ... was added to multiplexed product with Hi-Di™ Formamide (Thermo Fisher Scientific, Waltham, MA) to aid in denaturing ...
-
bioRxiv - Bioengineering 2022Quote: Voltage activity within human heart organoids was assessed using di-8-ANEPPS (Thermo Fisher Scientific). Di-8-ANEPPS was solubilized in DMSO per the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2023Quote: ... which were pre-loaded with 10 μL of Hi-Di™ formamide (ThermoFisher, Waltham, USA) and GeneScan™ -400HD ROX™ Size standard (ThermoFisher ...
-
bioRxiv - Immunology 2023Quote: ... Glycan samples were run with a LIZ 600 DNA ladder in Hi-Di formamide (ThermoFisher) on an ABI 3500xL DNA sequencer and analyzed with GlycanAssure Data Acquisition Software v.1.0 ...
-
bioRxiv - Physiology 2023Quote: Isolated atrial myocytes were stained with 4 µM di-4-ANEPPS for 10 minutes (Invitrogen) for membrane visualisation[3 ...
-
bioRxiv - Molecular Biology 2023Quote: ... One microliter of sample was diluted in 9 ul Hi-Di™ Formamide (Applied Biosystems) containing 0.25 ul GeneScan™ 600 LIZ™ Size Standard ...
-
bioRxiv - Cell Biology 2019Quote: ... First antibody incubation was done O/N at 4°C and the secondary HRP-coupled antibodies goat anti-Mouse IgG (H+L) (ThermoFisher scientific; 31430; 1:10,000) or polyclonal HRP-conjugated goat-anti-rabbit IgG (ThermoFisher scientific ...
-
bioRxiv - Molecular Biology 2021Quote: ... coupled to a Q Exactive hybrid mass spectrometer (Thermo) (N-terminal FLAG-tagged datasets) or an Orbitrap Fusion Lumos Tribrid (Thermo Fisher Scientific, Waltham, MA) mass spectrometer (C-terminal FLAG-tagged datasets) ...
-
bioRxiv - Microbiology 2020Quote: ... and resuspended in 0.1 ml of PBS supplemented with the lipophilic styryl membrane dye N-(3-triethylammoniumprpyl)-4-(p-diethylaminophenyl-hexatrienyl) pyridinium dibromide (FM4-64, Molecular Probes, Invitrogen; 10 μg.ml-1) (Pogliano et al. ...
-
bioRxiv - Physiology 2022Quote: Samples of frozen mouse liver were homogenized in lysis and extraction buffer (RIPA lysis and extraction buffer, catalog n° 89900, Thermofisher Scientific, Waltham, MA, USA) containing protease inhibitors (protease inhibitor cocktails tablets ...
-
bioRxiv - Physiology 2022Quote: ... equal amounts of the protein samples (20 μg of liver extracts) were resuspended in Laemmli sample buffer (catalog n°39000, Thermofisher Scientific, Waltham, MA, USA) and separated in an 4%–20% sodium dodecylsulfate (SDS ...
-
bioRxiv - Molecular Biology 2021Quote: ... the inserts of the entry vectors were recombined into the into the pDEST 5’ BirA*-FLAG-pcDNA5-FRT-TO destination vector (Invitrogen, cat n°12285-011) using LR-clonase (Invitrogen ...
-
bioRxiv - Molecular Biology 2021Quote: ... [30] at a concentration of 57.5 μM (5% of the suggested concentration in RPMI 1640 medium) and media were supplemented with 10% dialyzed fetal bovine serum (Invitrogen, cat n°26400-044). Cell populations were cultured for at least six population doublings for complete incorporation of the labelled L-Arg.
-
bioRxiv - Biophysics 2022Quote: ... 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride (EDC, Catalog No. 22980) and Sulfo-NHS (N-hydroxysulfosuccinimide, Catalog No. 24510) were purchased from Thermo Scientific (Waltham, MA, US). Phosphate-Buffered Saline (PBS ...
-
bioRxiv - Genomics 2020Quote: ... The qPCRs were run of both technical and biological replicates (n=3) using an ABI7500 sequence detection system (Applied Biosystems by Life Technologies, Darmstadt, Germany). Fold expression changes were determined using a standard 2−ΔΔCT method that compares CT (cycle threshold ...
-
bioRxiv - Developmental Biology 2019Quote: ... C- and N-terminal GFP-tagged Nubp1 and Nubp2 expression plasmids [32] were transfected using Lipofectamine® 3000 (Thermo Fisher Scientific, Waltham, MA, USA) and imaged using a Nikon C2 Confocal microscope.
-
bioRxiv - Physiology 2019Quote: ... Purified RNA (100 ng per sample) was labeled with the Whole-Transcript Sense Target Assay kit (Affymetrix, Santa Clara, CA, USA; P/N 900652) and hybridized to an Affymetrix Mouse Gene 1.0 arrays or 2.1 ST array plate (Affymetrix) ...
-
bioRxiv - Neuroscience 2019Quote: ... cycling synaptic vesicles were stained with the styryl dye N-(3-triethylammoniumpropyl)-4-(4-(dibutylamino) styryl) pyridium dibromide (FM1-43; Molecular Probes, Inc., Eugene, OR). For labeling of synaptic vesicles ...
-
bioRxiv - Biophysics 2021Quote: ... The silanized surfaces were incubated in 10 mg/mL sulfosuccinimidyl 4-(N-maleimidomethyl)cyclohexane-1-carboxylate (sulfo-SMCC, Thermo Fisher Scientific, Waltham, MA, USA) at room temperature for 30 min ...
-
bioRxiv - Neuroscience 2022Quote: The reverse transcription was performed with 500 ng of total RNA using the High-capacity cDNA reverse transcription kit (ThermoFisher Scientific, catalog n°4368814) according to the manufacturer’s instructions ...
-
bioRxiv - Bioengineering 2022Quote: ... A nanoViper Trap Column (C18, 3 μm particle size, 100 Å pore size, 20 mm × 75 μm ID; Thermo Fisher Scientific, P/N 164946) was used for desalting ...
-
bioRxiv - Bioengineering 2022Quote: ... Samples were run on a PepMap RSLC C18 column (2 μm particle size, 15 cm × 50 μm ID; Thermo Fisher Scientific, P/N ES901). A nanoViper Trap Column (C18 ...
-
bioRxiv - Biophysics 2022Quote: ... was spiked with 1% of Alexa 488-labeled HU-B mutant (containing an N-terminal Cys conjugated with Alexa Fluor™ 488 C5 Maleimide from Thermofisher; Cat no. A10254). The 488 nm laser was set at 100% for efficient bleaching and focused on a small circular region with a diameter of 1 μm ...
-
bioRxiv - Developmental Biology 2019Quote: ... The silicon device surfaces were functionalized with a previously described protocol using 3-mercaptopropyltrimethoxysilane (MPTMS) and N-gamma-maleimidobutyryloxysuccinimide ester (GMBS) NeutrAvidin-biotin chemistry (Thermo Fisher Scientific, Rockford, IL)[32 ...
-
bioRxiv - Developmental Biology 2020Quote: The retinas of juveniles (n = 6 retinas from 5 animals) and adults (n = 5 retinas from 4 animals) were dissected out and put in RNAlater (Ambion Inc., Austin, TX, USA). RNA extraction was performed using the RNeasy mini kit (Qiagen ...
-
bioRxiv - Microbiology 2020Quote: ... followed by incubation with rabbit antibodies against SFTSV-N as primary antibodies and HRP-conjugated recombinant protein A/G (Cat. No. 32490, Thermo Scientific, Rockford, IL, USA) as secondary antibodies ...
-
bioRxiv - Genetics 2020Quote: Guinea pig anti-zebrafish Rad21l1 polyclonal antibody production: An N-terminal fragment of Rad21l1 cDNA was amplified with Phusion DNA polymerase (Thermo Fisher Scientific, Catalog #: M0530L) using the following primers ...
-
bioRxiv - Plant Biology 2021Quote: ... C and N concentrations (mass based) of the samples (5 mg dry weight) were determined with Elemental analyzer (Flash EA 2000, Thermo Fisher Scientific, Bremen, Germany) in four biological replicates ...
-
bioRxiv - Cell Biology 2020Quote: ... n-dodecyl-β-D-maltoside and assessed for OxPhos complex content using a Native PAGE Novex Bis-Tris Gel system (Invitrogen, Carlsbad, CA, USA). The separated proteins were transferred to polyvinylidene fluoride membranes ...