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Citations for Thermo Fisher :
1801 - 1850 of 10000+ citations for 7' BROMO 2' 3' DIHYDRO 1'H SPIRO CYCLOPROPANE 1 4' ISOQUINOLINE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2019Quote: ... 3 PBS washes were performed and then 2 hours of incubation in CY-5 goat anti-rabbit (1:1000, Invitrogen, ThermoFisher Scientific, A10523) diluted in 2%NGS-PBS ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were rinsed (3 times) with 2 ml incubation buffer and resuspended with anti-mouse Alexa Fluor 647 secondary antibody (1:2000, Invitrogen, Carlsbad, CA, USA) for 60 minutes at RT ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were washed with cold 1× PBS-T for 3 times each for 10 min then incubated for 2 hours at RT with secondary antibodies (AF546-Ms: Invitrogen A11003, 1:500) diluted in 1× PBS-T ...
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bioRxiv - Neuroscience 2023Quote: ... in 1X PBS-0.3% Triton X-100 (0.3% PBST) before 2-3 day incubation with primary antibodies at 4C for PCP4 (1:250, Invitrogen Cat# PA5-52209, RRID:AB_2645298) or NECAB2 (1:250 ...
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bioRxiv - Developmental Biology 2024Quote: ... HaCaT cell transfection with a 75 pM siRNA mix (#2316-1, #2316-2, and #2316-3) was conducted using Lipofectamine RNAiMAX transfection reagent (Invitrogen, Carlsbad, CA, USA), following the manufacturer’s guidelines.
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bioRxiv - Bioengineering 2020Quote: ... DAPI ((4’,6-diamidino-2 phenylindole, Invitrogen) was added and samples were covered with coverslips ...
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bioRxiv - Immunology 2020Quote: ... and Fluo-4 AM (Invitrogen, 2 μM) probes for 30 min before fixation with 1% PFA for 4 hours ...
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bioRxiv - Developmental Biology 2022Quote: ... 4’,6-diamidino-2-phenylindole (DAPI, Invitrogen) was added to embryos (10 μg/mL in PBST ...
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bioRxiv - Genomics 2021Quote: ... 3.5× 10−4% 2-mercaptoethanol (Gibco, 21985023), 0.12% Nicotinamide (Calbiochem ...
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bioRxiv - Bioengineering 2022Quote: ... 4’,6-diamidino-2-phenylindole (DAPI; Invitrogen) allowed visualization of the nucleus (1:5000 dilution) ...
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bioRxiv - Molecular Biology 2021Quote: ... slides were washed thrice for 5 min in PBS before incubation with Alexa 594 goat-anti rabbit secondary antibody at 1:1,000 and Alexa Fluor 488 goat anti-human IgG(H+L) at 1:500 (both Invitrogen; in PBS) for 2 h at RT ...
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bioRxiv - Neuroscience 2019Quote: ... Sections were then incubated for 1 h using with secondary antibodies conjugated to Alex Fluor-546 or 647 of appropriate species (1:500, ThermoFisher Scientific). After several washes in PBS sections were mounted on glass slides using Fluoromount-G ...
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bioRxiv - Neuroscience 2019Quote: ... 1 mg of striatal lysates was pre-cleared for 1 h with 80 ul Protein G Sepharose coated beads (ThermoFisher Scientific), then centrifuged at 4°C for 5 min at 1000 rpm ...
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bioRxiv - Bioengineering 2021Quote: ... antibody at 5 μg mL−1 in BSA 1% m v−1 in PBS for 1 h at room temperature, then with anti-rabbit Alexa 568 (LifeTech, A11036) and Hoechst (Invitrogen, H3570) 1 μg mL−1 in BSA 1% m v-1 in PBS for 1 h ...
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bioRxiv - Microbiology 2020Quote: ... and incubated for 1 h at RT with a mixture of Alexa Fluor 594 conjugated anti-rabbit IgG (1:500; A11012; ThermoFisher, USA) and Alexa Fluor 488-conjugated anti-mouse IgG (1:500 ...
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bioRxiv - Genetics 2020Quote: ... then incubated with DAPI (1:5000) and Alexa Fluor 488-conjugated goat anti-rabbit IgG (H+L) secondary antibodies (1:200; Thermo Fisher). After incubation ...
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bioRxiv - Neuroscience 2020Quote: ... sections were washed 3 × 10 min with PBS and incubated for 1 h at room temperature with the secondary antibody (goat anti-rabbit Alexa Fluor 488, 1:1000; Invitrogen, Germany) diluted in blocking solution ...
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bioRxiv - Neuroscience 2021Quote: ... bound antibody was detected by incubation for 1 h with Alexa Fluor 546-IgGs or Alexa Fluor 488-IgGs (1:200 for both, Invitrogen, USA).
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bioRxiv - Cell Biology 2020Quote: ... cells were subsequently incubated with secondary antibodies for 1 h and nuclei visualized with 1 μg/ml DAPI (Invitrogen, D-1306). All incubations were performed at room temperature ...
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bioRxiv - Cancer Biology 2022Quote: ... sections were washed and incubated with for 1 h at room temperature with secondary antibodies goat anti-rabbit Alexa Flour 568 (1:1000, A11011; Life Technologies) for Nestin ...
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bioRxiv - Microbiology 2020Quote: ... followed by a 1 h incubation with a 1:3,000 dilution of goat anti-rabbit IgG coupled to HRP secondary antibodies (Thermo Fisher Scientific). Bands were detected by chemiluminescence using ECL Western blotting detection reagent (Amersham Bioscience ...
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bioRxiv - Biochemistry 2020Quote: ... 5 mM 2-mercaptoethanol and incubated for 1 h at 37 °C with 1 mM EDTA (ethylenediaminetetraacetic acid) and RNase A/T1 (ThermoFisher Scientific) (1:100) ...
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bioRxiv - Microbiology 2019Quote: ... A final washing step after 1 h incubation at TR was followed by addition of 100 µl 1-Step Slow TMB-ELISA substrate (Thermo Scientific) to each well and absorbance was measured after 10 min incubation at TR using the Bio-Rad Model 680 Microplate Reader (655 nm ...
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bioRxiv - Cell Biology 2019Quote: ... The membranes were washed for 15 min in TBST and then incubated for 1 h with HRP-conjugated secondary antibodies (1:5,000; Pierce Antibodies; Thermo Fisher Scientific) in TBST at RT ...
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bioRxiv - Cell Biology 2019Quote: ... in 0.5% BSA PBS at room temperature for 1 h prior to incubation with AlexaFluor 594 or 488 anti-rabbit secondary antibodies (1:5000, Molecular Probes) in 0.5% BSA PBS ...
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bioRxiv - Systems Biology 2019Quote: ... Alexa Fluor 488-conjugated goat anti-rabbit IgG and Alexa Fluor 647-conjugated goat anti-mouse IgG diluted in washing solution for 1 h (1:100, Thermo Fisher Scientific Inc. ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... and incubated in absence of light for 1 h with fluorescent goat anti-mouse (1:500, Alexa Fluor 488, Life Technologies) diluted in TBS with 3% BSA ...
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bioRxiv - Physiology 2021Quote: ... The muscle sections were then washed three times in PBS before being incubated for 1 h in the dark at room temperature with Alexa Fluor 488 IgG secondary antibody (A-11008, 1:500; ThermoFisher Scientific) diluted in blocking solution ...
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bioRxiv - Microbiology 2021Quote: ... diluted 1:3000 in 1% PBS-milk supplemented with 0.1% Tween-20 for 1 h at 37C and detected using 1-Step Ultra TMB-ELISA Substrate Solution (Thermo Fisher Scientific). Plates were read using a Cytation 5 imager (BioTek ...
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bioRxiv - Molecular Biology 2023Quote: ... The anti-FLAG antibody was detected using an anti-mouse Alexa 647 secondary antibody (Alexa 488 goat anti-mouse, 1:500 dilution, RT, 1 h, Invitrogen, #A11029). The images were captured with an InCell2000 imager.
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bioRxiv - Neuroscience 2022Quote: ... RRID: AB_2893433), tdTomato (SICGEN, 1:500, RRID: AB_2722750) donkey anti-goat IgG (H+L) Alexa Fluor 488 (Invitrogen, 1:1000, A11057), Cy3 donkey anti-rabbit IgG (H+L ...
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bioRxiv - Neuroscience 2023Quote: Age synchronized worms were washed thrice with 500 μl M9 buffer in 1.5 ml microfuge tube and incubated in dark for 1 h in 1 ml M9 containing 5 µl DiD dye (Vybrant™ DiD Cell-Labeling Solution, ThermoFisher) at ∼20-30 rpm ...
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bioRxiv - Cancer Biology 2023Quote: ... slides were washed with TBST and incubated with the required fluorescent-dye conjugated secondary antibody (1:1000) for 1 h at RT (Alexafluor, Invitrogen, Thermofisher). Slides were mounted with DAPI Pro-LongDiamond antifade mountant overnight and analyzed the next day ...
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bioRxiv - Cell Biology 2023Quote: ... HRP-conjugated secondary antibodies were used at 1:1000 at RT for 1 h with rotation (Anti-rabbit-HRP, Invitrogen, anti-Armenian Hamster-HRP, Thermo Fisher). Blots were imaged using an Amersham Imager 600 ...
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bioRxiv - Bioengineering 2022Quote: ... sections were incubated for 1 h at RT with goat anti-mouse Alexa 555-conjugated antibody (1:300; A21434, Life Technologies). After counterstaining with hematoxylin slides were mounted under coverslips in Prolong Gold anti-fade reagent (Life Technologies) ...
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bioRxiv - Cancer Biology 2023Quote: ... for 1 h (Western blot) or 30 min (MS) at 170 V in 1× NuPAGE MOPS SDS running buffer (Thermo Scientific). The Colloidal blue staining kit (Thermo Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were then incubated for 1 h at room temperature with anti-rabbit Alex Fluor 546 (1:1000, Thermo Fisher Scientific). Images were acquired using the Keyence BZ-X800.
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bioRxiv - Neuroscience 2023Quote: ... the tissues sections were washed with PBS and then incubated for 1 h at room temperature with the following secondary antibodies: anti-mouse Alexa Fluor 488 (1:600, ThermoFisher Scientific), anti-goat Alexa Fluor 555 (1:1000 ...
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bioRxiv - Cell Biology 2023Quote: ... multicilia were labeled with 100 nM SiR-tubulin for 1 h and small fluorescent latex beads with a diameter of 250 nm at 1:500 dilution (Invitrogen, F8809) were added to the culture medium ...
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bioRxiv - Neuroscience 2023Quote: ... for 1 h at room temperature and then exposed to antibodies specific for human PD-1 (ThermoFisher Scientific; 14-9969-82), Iba1 (Wako Chemicals ...
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bioRxiv - Plant Biology 2024Quote: ... the membrane was blocked with 5% bovine serum albumin in PBS with 0.05% Tween-20 for 1 h and then incubated with HRP-conjugated streptavidin (1:20000, Thermo Fisher Scientific) with shaking for 1 h at room temperature or 4°C overnight ...
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bioRxiv - Microbiology 2024Quote: ... Membranes were washed 3 times with TBS-T and incubated for 1 h at room temperature with an HRP-conjugated anti-mouse (dilution 1:50,000, Invitrogen, G-21040) or anti-rabbit (dilution 1:50,000 ...
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bioRxiv - Immunology 2021Quote: ... iBMDMs were incubated for 3 h in 50 μM of the mitochondrial uncoupler carbonyl cyanide 3-chlorophenylhydrazone (CCCP; ThermoFisher, M20036). Control samples included ...
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bioRxiv - Neuroscience 2022Quote: ... they were incubated for 2 h at RT with an Alexa Fluor 555 nm-conjugated goat anti-mouse secondary antibody (1:500; Molecular Probes, ThermoFisher Scientific cat#A21127; RRID: AB_141596) in blocking solution ...
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bioRxiv - Cell Biology 2020Quote: ... in a 3:1 ratio with 1X penicillin/streptomycin (Gibco; 15070) and 5% FBS (Gibco ...
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bioRxiv - Neuroscience 2021Quote: ... rat Taste receptor type 1 member 3 (Tas1r3, Rn00590759_g1, Applied Biosystems) and rat Taste receptor ...
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The tumour microenvironment shapes dendritic cell plasticity in a human organotypic melanoma culturebioRxiv - Immunology 2019Quote: ... in Fibroblast medium (3:1 DMEM: Ham’s F12 Nutrient Mixture, Gibco) supplemented with 10% FCS ...
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bioRxiv - Cancer Biology 2019Quote: ... Sections were counterstained with ToPro-3 (1:1000 dilution; Life Technologies).
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bioRxiv - Cell Biology 2020Quote: ... and washed 3 times with 1x PBS (Fisher Scientific, BP399-1).
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bioRxiv - Microbiology 2020Quote: ... and anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (1:5,000 dilution, Invitrogen). Secondary antibodies against rabbit and mouse IgG conjugated to IRDye 680LT or IRDye 800CW were obtained from Li-Cor (1:10,000 dilution) ...