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Citations for Thermo Fisher :
1701 - 1750 of 10000+ citations for 7' BROMO 2' 3' DIHYDRO 1'H SPIRO CYCLOPROPANE 1 4' ISOQUINOLINE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2019Quote: ... The pellet was resuspended 3 times using 7 mL Wheaton Dounce tissue grinder (Fisher Scientific) and centrifuged at 12,000 rpm at 4°C for 30 minutes (Sorvall SS-34 centrifuge rotor) ...
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bioRxiv - Genetics 2020Quote: ... 25 μL of media containing STS and CellEvent Caspase-3/7 Green Detection Reagent (ThermoFisher) at final treatment concentrations of 1 μM and 2.5 μM ...
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bioRxiv - Neuroscience 2022Quote: ... The electrodes were labelled with DiIC18(7) (1,1’-Dioctadecyl-3,3,3’,3’-Tetramethylindotricarbocyanine Iodide) (DiR) (Invitrogen) to enable post-mortem reconstruction of the electrode tracks in histological sections.
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bioRxiv - Immunology 2022Quote: ... Alexa Fluor 488 and caspase-3/7 activity detection dyes were purchased from Thermo Fisher, and Viobility™ 405/452 Fixable Dye from Miltenyi Biotec.
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bioRxiv - Microbiology 2023Quote: ... Apoptosis assays were perfomed using CellEvent Caspase 3/7 Green Detection Reagent (Thermo Fisher Scientific) and Live/Dead eFluor 660 (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were stained with CellEvent Caspase-3/7 Green Flow Cytometry Assay Kit (ThermoFisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... 7-Aminoactinomycin D (7-AAD) (Invitrogen) at 1:400 was used as a marker for dead cells ...
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bioRxiv - Molecular Biology 2022Quote: A concentration of 6 × 103 cells was loaded with 5 mM 4-amino-5-methylamino-2’,7’- difluorofluorescein diacetate (DAF-FM, Molecular Probes, Thermo Fisher, Sao Paulo, Brazil) after 72 h of treatment ...
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bioRxiv - Microbiology 2021Quote: ... siDDX42-4: 5’-AUCUCGAAUACCCUUUACG-3’ (ID:136410, Ambion®).
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bioRxiv - Microbiology 2023Quote: 3’(4-Hydroxyphenyl)-fluorescein (HPF; Molecular Probes, OR, USA) was used for detecting •OH production (Avci et al. ...
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bioRxiv - Microbiology 2024Quote: ... Propidium iodide (PI) and the pH sensitive 2’,7’-Bis-(2-Carboxyethyl)-5-(and-6)-Carboxyfluorescein (BCECF) (Invitrogen) were added to these at a final concentration of 100µM and 10µM respectively ...
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bioRxiv - Neuroscience 2020Quote: ... serum-free medium (DMEM:F12 at 2:1) supplemented with N2 (1% v/v; Invitrogen), 1mM L-glutamine ...
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bioRxiv - Neuroscience 2023Quote: ... supplemented with 2% B27 and 1% L-glutamine and 1% Pen-Strep (Life Technologies). Dissociated cortical neurons were infected with Control and Ncan shRNA AAVs at 7 days in vitro (DIV ...
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bioRxiv - Neuroscience 2023Quote: ... Alexa647 ICAM-2 at 1:100 and Live/Dead at 1:1000 (L34962, Invitrogen), for sorting of CD45neg ...
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bioRxiv - Neuroscience 2024Quote: ... supplemented with 2% B27 and 1% L-glutamine and 1% Pen-Strep (Life Technologies). Dissociated wildtype and Ncan KO cortical neurons were initially infected with Control and the two Ncan shRNA AAVs at 7 days in vitro (DIV ...
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bioRxiv - Biochemistry 2024Quote: ... Solutions were further diluted to 1 µM using 2:1 MeOH:CHCl3 (ThermoFisher Scientific, Australia) with 5 mM ammonium acetate (SigmaAldrich ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... #2: 5’-gatcccGATTACTCGGCCATGATCAAAgcttcctgtcacTTTGATCATGGCCGAGTAATCttt ttta-3’ and 5’-agcttaaaaaaGATTACTCGGCCATGATCAAAgtgacaggaagcTTTGATCATGGCCGAGTAATgg-3’ were purchased from Invitrogen. The DNA oligo pairs were annealed and inserted into pEntryCla12-chickU6 shuttle vector using BamHI/HindIII site.
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bioRxiv - Microbiology 2023Quote: ... niveus CO7 DNA ranging from 7 to 7 × 10−4 ng/µl was generated and quantified using a Nanodrop-8000 Spectrophotometer (Thermo Scientific, Wilmington DE, USA) (260/280 ratio of 1.89) ...
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bioRxiv - Immunology 2022Quote: ... for 4 h and the FOXP3 / Transcription Factor staining buffer set (Invitrogen) was used according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2019Quote: ... and 48 h post-transfection selected in 4 µg/ml puromycin (Invitrogen). Stable cell lines were maintained in DMEM (see ‘Cell lines and tissue culture conditions’ ...
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bioRxiv - Cancer Biology 2019Quote: ... and 48 h post-transfection selected with 4 µg/ml puromycin (Invitrogen). The clones were sorted by FACS and screened for target mRNA knockdown by Western blot or qPCR analysis (see ‘Western blot analysis and antibodies’ and ‘Quantitative real-time PCR (qPCR ...
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bioRxiv - Physiology 2021Quote: ... for 12-16 h at 4°C followed by 0.05% trypsin (Gibco) for 10-20 min at 37°C ...
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bioRxiv - Microbiology 2023Quote: ... After 4 h incubation with Protein AAgarose (20333, Thermo Scientific, Shanghai, China) at 4 °C ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were washed 2-3 times (200 rpm, 5 min at 4°C) in eBioscience™ Permeabilization buffer (250 µl/well) (Invitrogen) and resuspended in eBioscience™ Fixation/Permeabilization solution (Invitrogen ...
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Enzymatic RNA Biotinylation for Affinity Purification and Identification of RNA-protein InteractionsbioRxiv - Biochemistry 2020Quote: HeLa cell pellets (∼2×107) or MCF7 cell pellets (∼4×107) were lysed with 3 mL Mammalian Protein Extraction Reagent (Thermo Scientific), supplemented with 2X HALT protease inhibitor (Thermo Scientific) ...
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bioRxiv - Cell Biology 2021Quote: ... were transiently transfected into 8 × 104 U2OS 2-6-3 cells in 4-well chamber slides using Lipofectamine 2000 reagent (Invitrogen, 11668019) according to the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2022Quote: ... 3-4 mg total protein and 2 ug of KIF1C antibody or 10 ug mCherry antibody (Invitrogen mCherry Monoclonal Antibody (16D7)) were used ...
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The effects of caloric restriction on adipose tissue and metabolic health are sex- and age-dependentbioRxiv - Physiology 2023Quote: ... Tissues in formalin were fixed at 4°C for 2-3 days before being washed and stored in DPBS (Life Technologies). Tissues on dry ice were stored at −80°C prior to downstream analysis.
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bioRxiv - Microbiology 2020Quote: ... and resuspended in 0.1 ml of PBS supplemented with the lipophilic styryl membrane dye N-(3-triethylammoniumprpyl)-4-(p-diethylaminophenyl-hexatrienyl) pyridinium dibromide (FM4-64, Molecular Probes, Invitrogen; 10 μg.ml-1) (Pogliano et al. ...
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bioRxiv - Microbiology 2019Quote: ... 1/10 volume of 3 M Sodium Acetate (pH 5.2) and 1 μl of GlycoBlue (Life Technologies). The precipitate was pelleted by centrifugation (30 min ...
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bioRxiv - Microbiology 2020Quote: ... 1/10 volume of 3 M Sodium Acetate (pH 5.2) and 1 μl of GlycoBlue (Life Technologies). The precipitate was pelleted by centrifugation (30 min ...
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bioRxiv - Genetics 2023Quote: ... Induction 3 Medium (StemPro-34 complete media, 20 mM HEPES, 1% GlutaMAX, 1% Penicillin-Streptomycin (Life Technologies); 213 μg/mL 2-phosphate Ascorbic Acid (Sigma-Aldrich) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... into the packaging 293T cells (with molar ratio for Luc-T20: N: M/E: S as 3: 1: 1: 1) with LipofectamineTM 2000 (Thermo Fisher Scientific). At 24 and 48 hours post-transfection ...
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bioRxiv - Microbiology 2020Quote: ... MH96 was grown overnight (18 h) in 3 ml Luria Broth Base (Invitrogen) with shaking (250 rpm ...
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bioRxiv - Neuroscience 2021Quote: ... the culture medium was replaced 3 h after plating by Opti-MEM (Invitrogen) before the DNA mixture was added ...
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bioRxiv - Biochemistry 2022Quote: ... The cleared lysates were incubated for 2 h at 4°C with IgG-coupled Dynabeads (Dynabeads M-270 Epoxy; Invitrogen, Thermo Fisher Scientific, Basel, Switzerland). The beads were washes 5 times with lysis buffer and proteins were eluted in TEV buffer (50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Biochemistry 2022Quote: ... the cleared lysates were incubated for 2 h at 4°C with prewashed anti-c-myc MagBeads (Pierce Thermo Fisher Scientific, product number 88843). After five washes with lysis buffer ...
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bioRxiv - Microbiology 2023Quote: ... Cells from two separate transects were counted from a 4-well dish with inverted light microscope (Zeiss PrimoVert) after 2 h of settling (Nunc, Thermo Fisher Scientific, US). Depending on the culture density up to 1 L of sample was filtered through each (n = 5 per sampling day ...
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bioRxiv - Neuroscience 2021Quote: ... the sections were washed three times in PBS and incubated during 4 h in a blocking solution containing a secondary antibody to reveal TH (donkey anti-rabbit Alexa Fluor 594, Invitrogen A21207 lot 1890862 and 2145022 (1:400), RRID ...
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bioRxiv - Neuroscience 2021Quote: ... was added to halt the proteolysis and samples were centrifuged at 300xG for 10 minutes at 4°C and resuspended in a 1:1:10 solution of DNase:ovomucoid:Neurobasal media (Worthington:Worthington:Gibco). Cell suspensions were incubated with primary antibodies to CD11b and CD45 (table 1 ...
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bioRxiv - Biophysics 2023Quote: ... 4 μl of 1 μm FluoSpheres (1 to 800 dilution, 585/608 nm, ThermoFisher Scientific, F13083), were applied to the grid in the humidity chamber of the Leica EM GP plunger prior to the vitrification step ...
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bioRxiv - Molecular Biology 2022Quote: ... Cultures were passaged every 5 to 7 days with collagenase type IV (Invitrogen; 1 mg/mL). The identities of all parental hESC and hiPSC lines were confirmed by DNA fingerprinting and all cell lines were regularly tested to exclude mycoplasma contaminations using a PCR-based assay ...
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bioRxiv - Microbiology 2021Quote: ... 1% Penicillin/Streptomycin and 7% of heat-inactivated fetal bovine serum (FBS) (all from ThermoFisher Scientific). VeroE6/TMPRSS2 cells (NIBSC 100978 ...
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bioRxiv - Developmental Biology 2021Quote: ... 1 minute at 60°C)] on a QuantStudio 5 or QuantStudio 7 qPCR machine (Applied Biosystems). For qPCR assessments ...
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bioRxiv - Biochemistry 2023Quote: ... 1 mM DTT using ‘SnakeSkin’ dialysis tubing with a 7 kDa molecular weight cut-off (ThermoFisher). His6-tagged cleavage products as well as TEV protease were removed with Ni-NTA agarose resin (Qiagen ...
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bioRxiv - Cell Biology 2023Quote: ... Integrin αV was stained with a commercial antibody (ThermoFisher, USA, clone RMV-7, 1:1000 diluted). Labeling of primary antibodies was visualized with anti-mouse PE and anti-rat PE (both Southern Biotech ...
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bioRxiv - Synthetic Biology 2023Quote: ... Huh-7 and TCMK-1 cells were maintained in high glucose DMEM media (Gibco, 41966-029) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Immunology 2024Quote: ... Anti-CD49-PerCPefluor710 (TS2/7) and anti-PD-1-PeCy7 (eBioJ105) were supplied by Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2020Quote: ... we loaded the cells with 2’,7’-dichlorodihydrofluorescein diacetate (H2DCFDA, Molecular Probes®) and incubated at 37°C ...
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bioRxiv - Bioengineering 2021Quote: ... Samples were stained with 2’,7’-Dichlorofluorescein diacetate (10 µM) (Thermo Fisher, USA) for one hour ...