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Citations for Thermo Fisher :
1601 - 1650 of 10000+ citations for 7' BROMO 2' 3' DIHYDRO 1'H SPIRO CYCLOPROPANE 1 4' ISOQUINOLINE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... cover slips were incubated for 1 h at room temperature with mouse anti-mouse Keratin 14 (LL02, Thermo Fisher, MA5-11599, 1:100 – 1:10), rabbit anti-mouse Keratin 5 (BioLegend ...
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bioRxiv - Cell Biology 2020Quote: ... and TagRFP-T-VAPB(1-218)-eMagB or TagRFP-T-eMagB-PHOSBP (prey) and iRFP-P4C at a 3:2:1 ratio in OptiMEM-I (Thermo Fisher Scientific) (1:4 DNA ...
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bioRxiv - Cell Biology 2019Quote: ... RNA interference was done by transfecting 100 nM siRNA (TPD54 1: GUCCUACCUGUUACGCAAU, TPD54 2:CUCACGUUUGUAGAUGAAA, TPD54 3:CAUGUUAGCCCAUCAGAAU) with Lipofectamine 2000 (Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2020Quote: ... N-((6-(2,4-DNP)Amino)Hexanoyl)-1-(BODIPY™ FL C5)-2-Hexyl-Sn-Glycero-3-Phosphoethanolamine (PED-A1) and BODIPY™ FL C5 were purchased from Thermofisher scientific ...
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bioRxiv - Biochemistry 2021Quote: ... approximately 1 pmol injected onto a Acclaim PepMap 100 guard column (75 μm×2 cm C18, 3 μm particles, 100 Å; Thermo Scientific) and separated on a 50 cm PepMap RSLC C18 (75 μm×50 cm ...
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bioRxiv - Microbiology 2020Quote: ... incubating with primary antibodies (rabbit anti-β-actin, 1:1000, Thermo Fisher Scientific, PA1-183; mouse anti-claudin-2, 3:500, Invitrogen, 325600 ...
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bioRxiv - Bioengineering 2019Quote: ... medium was switched to a B27-based Retinal differentiation medium (BRDM) (DMEM/F12 (3:1) with 2% B27 (w/o vitamin A, ThermoFisher Scientific, USA), 1x NEAA and 1x AA) ...
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bioRxiv - Microbiology 2020Quote: ... were performed on an Ultimate 3000 RSLC nano instrument coupled to either a QExactive Plus (M#1, M#3) or an HF mass spectrometer (M#2; Thermo Fisher Scientific) as described previously.24 Tryptic peptides were trapped for 4 min on an Acclaim Pep Map 100 column (2 cm × 75 μm ...
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bioRxiv - Physiology 2023Quote: ... IALVs were then washed with PBS containing 0.1% Triton X-100 (PBST) 3 times and blocked for a minimum of 2 hr with Blockaid® (B-10710, ThermoFisher Scientific). IALVs were then stained with the corresponding primary antibodies in BlockAid® Solution ...
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bioRxiv - Biochemistry 2021Quote: ... 6,8-Difluoro-4-Methylumbelliferyl Phosphate (DiFMUP, #D22065) and 6,8-difluoro-7-hydroxy-4-methylcoumarin (DiFMU, #D6566) were purchased from Life Technologies.
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bioRxiv - Developmental Biology 2020Quote: Transfection mix was prepared by combining PEI and plasmid DNA (4:1 ratio; 4 µg PEI per 1 µg DNA) in Opti-MEM™ Reduced Serum Medium (Thermo Fisher Scientific) followed by brief vortexing ...
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bioRxiv - Developmental Biology 2021Quote: ... samples were blocked for 1 hour in 2 mg ml−1 BSA and 1% Goat Serum (Thermo Fisher) in 1X PBSTXD (1X PBS ...
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bioRxiv - Immunology 2024Quote: ... CD206-FITC (19.2, 1:100), CD11c-PE (3.9, 1:50) and CD14-PerCP-Cy5.5 (61D3, 1:200) from eBiosciences (ThermoFisher) were used.
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Retinal affectation in Huntington’s disease mouse models concurs with a local innate immune responsebioRxiv - Neuroscience 2023Quote: ... sections were washed and incubated for 2 h with secondary antibodies conjugated to Alexa-488 (1:2000; Molecular Probes, Thermo Fisher Scientific, Waltham, MA, USA). For the D7F7 antibody ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were incubated for 2 hours at room temperature with goat anti-chicken IgY (H+L) Alexa Fluor™ 488 (Invitrogen, A-11039, 1:1000) diluted in TBS with 10% Serum ...
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bioRxiv - Cell Biology 2020Quote: ... samples were incubated with the following secondary antibodies for 1 h: Alexa 546-conjugated goat anti-rabbit IgG (1:1,000, Thermo Fisher Scientific Cat# A-11010 ...
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bioRxiv - Developmental Biology 2020Quote: ... the cells were washed 3 times in PBS and incubated for 1 h in the dark with the following secondary antibodies: Alexa Fluor 594 (1:200, Cat# A11037, Invitrogen) or Alexa Fluor 488 (1:200 ...
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bioRxiv - Molecular Biology 2020Quote: ... reverse-transcribed at 50°C for 1 h ourwith SuperScript III and 1 mM of each dNTP (SuperScript III cDNA synthesis kit, Invitrogen) followed by 18-cycle PCR amplification.
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bioRxiv - Bioengineering 2019Quote: ... in 0.1% BSA in PBS) for 1 h, 37 °C, and then DAPI (1:1000, in 0.1% BSA in PBS) (Invitrogen, USA) was used for 5 min at room temperature to stain the nuclei ...
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bioRxiv - Cell Biology 2021Quote: ... and then incubated for 1 h at room temperature with Alexa 555-conjugated anti-rabbit IgG (1:100 dilution; ThermoFisher) in Can Get Signal immunostain (solution A) ...
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bioRxiv - Neuroscience 2021Quote: ... sections were incubated for 1 h at RT with the corresponding goat anti-rabbit Alexa Fluor 488 (1:250, ThermoFisher), donkey anti-rabbit Alexa Fluor 647 (1:250 ...
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bioRxiv - Neuroscience 2020Quote: ... sections were rinsed with PBST before 1 h incubation at room temperature with a secondary antibody conjugated to Alexa Fluor 488 (1:500; Invitrogen). After several washes ...
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bioRxiv - Neuroscience 2019Quote: ... The sections were then incubated for 1 h in Alexa 488 goat anti-chicken antibody (Life Technologies, #A11039; 1:500) in TBS containing 0.2% Triton X-100 and 2% normal goat serum ...
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bioRxiv - Neuroscience 2020Quote: ... cells were incubated for 1 h at room temperature with second antibodies conjugated to Alexa dyes (1:1000, Molecular Probes) and washed again ...
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bioRxiv - Neuroscience 2021Quote: ... and incubated for 1 h in 0.1 M PBS-Tx 0.2% containing 10% normal goat serum (1:100 ThermoFisher, Melbourne, Australia) to block non-specific binding ...
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bioRxiv - Cell Biology 2022Quote: ... tissues were incubated for 1 h at RT in darkness with the secondary antibodies (1/200; Alexa 488, Alexa 568 and Alexa 647; Invitrogen) and DAPI (4’,6-diamino-2-phenylindole ...
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bioRxiv - Biochemistry 2022Quote: ... The lysate is then clarified by centrifugation at 10,000 rev min−1 for 1 h using a Sorvall centrifuge (Thermo Scientific) followed by filtration via 0.45µm syringe filters ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were washed with PBS and incubated for 1 h at room temperature with AlexaFluor antibodies (Invitrogen, dilution 1:300) and Hoechst 33342 (Invitrogen ...
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bioRxiv - Genomics 2022Quote: ... for 1 h at 37°C and then diluted 1:20 in 50 μg/mL yeast tRNA (cat #Am7119, Invitrogen). A known concentration of plasmid DNA used to prepare the virus was also diluted 1:200 in 50 μg/mL yeast tRNA and processed in parallel ...
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bioRxiv - Biophysics 2022Quote: ... The lysate was then clarified by centrifugation at 10,000 rev min−1 for 1 h using a Sorvall centrifuge (Thermo Scientific) followed by filtration via 0.45μm syringe filters ...
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bioRxiv - Cell Biology 2022Quote: ... and incubated for 1 h at RT with Cy5-conjugated goat anti-rabbit antibody (1:250, #A10523, Thermo Fisher Scientific) diluted in blocking buffer ...
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bioRxiv - Neuroscience 2021Quote: ... all wells were processed for 1 h with appropriate fluorescent secondary antibodies (Alexa Fluor 594 donkey anti-rabbit (1: 1,500, Life Technologies); Alexa Fluor 488 donkey anti-chicken (1:500 ...
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bioRxiv - Cell Biology 2022Quote: ... followed by washing with Tween-20/PBS and 1 h incubation with HRP-conjugated goat anti-mouse IgG at 1:40 000 (Cat# A16066, Invitrogen) in 100 µl per well of Tween-20/PBS ...
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bioRxiv - Bioengineering 2022Quote: ... Alexa Fluor 488 goat anti mouse IgG (H+ highly cross adsorbed secondary anti body, Hoechst 3334333422 (1(1 mg/mL)mg/mL) were purchased from ThermoFisher Scientific ...
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bioRxiv - Biochemistry 2020Quote: ... and then for 1 h with a 1/10 000 dilution of anti-mouse– horseradish peroxidase conjugate (Thermo Fisher Scientific). His-tagged proteins bound to the membrane by virtue of its interaction with lipid ...
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bioRxiv - Cell Biology 2019Quote: ... and blocked for 1 h with PBS with 1% w/v bovine serum albumin (BSA) Fraction V (ThermoFisher, Waltham, MA) (58) ...
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bioRxiv - Molecular Biology 2020Quote: ... Samples were washed in PBS before incubation with mouse anti-BrdU (B44, Becton Dickinson 347580, 1:200) for 1 h and subsequently with antimouse IgG AlexaFluor 488 (ThermoFisher) for 1 h ...
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bioRxiv - Evolutionary Biology 2021Quote: ... and 1 h with the secondary antibody which was donkey anti–mouse coupled to Alexa Fluor 488 (Invitrogen, 1:500). Alternatively ...
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bioRxiv - Immunology 2021Quote: ... plasmids in the presence of pPPE36 (1 μg) or control plasmid (1 μg) for 24 h using Lipofectamine 2000 reagent (Invitrogen). And then cells were treated with TNF-α (20 ng/ml ...
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bioRxiv - Cell Biology 2022Quote: ... sections were washed and incubated for 1 h with Alexa Fluor conjugated secondary antibodies (1:1000 in blocking solution; Invitrogen). Following extensive washing ...
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bioRxiv - Cell Biology 2022Quote: ... cells were plated and 24 h later transfected with 1:1 ratio (DNA plasmid:Lipofectamine) using Lipofectamine 3000 reagent (ThermoFisher Scientific) following manufacturer recommendations ...
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bioRxiv - Immunology 2022Quote: ... The sections were then incubated for 1 h at RT with the corresponding fluorescent secondary antibody diluted in staining buffer (1:1000: Invitrogen), the cell nuclei were stained with Hoechst (10 µg/mL ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were washed three times in 1× PBSt and incubated for 1 h at room temperature with secondary antibodies labeled with Alexa fluorophores (Invitrogen) diluted in 1× PBSt (1:500) ...
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bioRxiv - Microbiology 2022Quote: ... The plates were washed three times with PBS with 0.05% Tween 20 and then incubated for 1 h at RT with horseradish peroxidase (HRP)-conjugated anti-mouse IgG (1:5,000, Cat# 62-6520, ThermoFisher Scientific), IgG1 (1:5,000 ...
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bioRxiv - Neuroscience 2023Quote: ... the sections were incubated for 1 h at room temperature with AlexaFluor-546-conjugated donkey anti-rabbit IgG (1:500, Invitrogen). Sections were then mounted using vector anti-fading mounting medium ...
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bioRxiv - Cancer Biology 2023Quote: ... slides were washed with TBST and incubated with the required fluorescent-dye conjugated secondary antibody (1:1000) for 1 h at RT (Alexafluor, Invitrogen, Thermofisher). Slides were mounted with DAPI Pro-LongDiamond antifade mountant overnight and analyzed the next day ...
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bioRxiv - Cell Biology 2023Quote: ... HRP-conjugated secondary antibodies were used at 1:1000 at RT for 1 h with rotation (Anti-rabbit-HRP, Invitrogen, anti-Armenian Hamster-HRP ...
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bioRxiv - Biochemistry 2023Quote: ... membranes were incubated at RT for 1 h with 1:1000 dilution of horseradish-peroxidase conjugated rabbit anti-guinea pig IgG secondary antibody (Invitrogen), or with 1:2500 dilution of horseradish-peroxidase conjugated donkey anti-mouse IgG secondary antibody (Jackson ImmunoResearch ...
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bioRxiv - Cell Biology 2022Quote: ... After washing for 20 minutes in PBS–BSA the samples were incubated for 1 h at room temperature with a goat anti-mouse secondary antibody conjugated with Alexa Fluor 488 (1:800, Invitrogen). DNA was visualized by incubation of 3–4 min in Hoechst 33342 dye (1µg/ml) ...
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bioRxiv - Microbiology 2023Quote: ... followed by a 1 h incubation in 1:5,000 goat α-rabbit HRP conjugated antibody (Thermo Fisher Scientific, Waltham, MA) in PBS ...